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1 
1.2 
Nutrition and 
Biomolecules 
Food Chemistry
2 
Functions of food 
1. Energy source – 90% used to make 
ATP 
2. Materials Source - for growth, repair, 
defence 
3. Continuity – replacement of cells and 
reproduction 
4. Metabolism - maintaining cells at 
high level of chemical efficiency
3 
• Metabolism - all reactions in living body 
• Anabolism building up of substances – input 
of energy required 
• E.g. photosynthesis, DNA replication, RNA 
formation, muscle building 
• Catabolism – breaking down of complex 
molecules – e.g. digestion 
• Enzymes protein biological catalysts, 
catalyse reactions [speed up – not used up in] 
• Respiration – release of energy from food
4 
Protein 
• Chemical elements C, H, O, N also P and 
S 
• Biomolecular sub-units – Amino Acids [20] 
• Linked by peptide bonds 
• One or more chains 
• Differ in sequence and number of a.a.’s 
• Made in ribosomes 
• Sources meat, fish, eggs, milk, cheese, 
pulses
5
6 
Roles 
• Structural 
• Keratin in hair, skin – protects 
• Myosin in muscle 
• Metabolic 
• Enzymes – protein biological catalysts
7 
Other roles 
• Antibodies 
• Haemoglobin 
• Chlorophyll 
• Insulin 
• Collagen
Shapes 
• Globular or Fibrous 
• Amino acids join together at angles 
• so as the protein forms it becomes folded 
• It can form a long fibre 
• Or it can become a ball 
• The function of a protein (enzyme) depends on 
its shape 
• If we heat much above 60oC or shake it 
become denatured i.e. It loses its function 
8
9 
Carbohydrate 
Chemical elements C, H, O [ H:O ratio 2:1 ] 
Monosaccharides [1 sugar unit] 
Glucose [C6H12O6], fructose, galactose 
Disaccharides [2 sugar units] 
Maltose, sucrose [C12H22O11], lactose 
Polysaccharides [many sugar units] 
Starch [plants], glycogen [animals]
10 
Types of carbohydrates 
Sugar 
• Found in honey, jam, sweets , fizzy drinks, 
alcohol. 
• [Most processed foods have sugar added] 
• Provides energy [quickly]
11 
Carbohydrates continued 
Starch 
• Found in potatoes, flour, bread, pasta, 
rice, cakes etc. 
• Provides energy [ more slowly over a 
longer period] 
• Test for with iodine which goes from 
brown to blue/black if starch is present.
12 
Carbohydrates continued 
Fibre [cellulose] 
• Found in whole-grain cereals [e.g. 
Weetabix, Shredded Wheat etc.] 
and vegetables. 
• Function to retain water and prevent 
constipation and also cancer of the 
bowel.
13 
Carbohydrate Roles 
Structural 
• Cellulose – plant cell walls – glued together 
to make rigid structure 
• Chitin – cell walls of fungi, insect cuticles 
Metabolic 
• Energy source – respiration of glucose 
• Energy store 
– Starch in plants 
– Glycogen in animals
14 
Lipids [fats] 
• Chemical Elements C, H, O 
• Glycerol + 3 fatty acids 
• Fat solid at RTP 
• Oil liquid at RTP 
• Lipids rich in saturated fatty acids [found 
mainly in animal fats] are associated with 
heart disease 
• Sources – meat, milk, butter, cheese, 
olives, maize, sunflower seeds
15 
Fatty Acid 
Glycerol Fatty Acid 
Fatty Acid 
Phospholipid 
Glycerol 
Fatty Acid 
Fatty Acid 
Lipid 
PO4
16 
Roles 
Structural 
• Phospholipid forms plasmalemma 
• Insulates organs e.g.kidney 
• Cuticle of leaves 
Metabolic 
• Storage of excess carbohydrate 
• Energy by respiration 
• Hormones – some are lipids 
• Stores fat soluble vitamins e.g. D
17 
Other 
• Heat Energy store – twice energy of 
carbohydrates per gram 
• insulation – sub-cutaneous layer 
• Protection – pads on feet and hands 
• Waterproofing – sebum on hair and skin 
• Storage – adipose tissue
18 
Vitamins 
• Non-protein substance required in tiny 
amounts for healthy metabolism 
• Vitamin C [Ascorbic Acid] 
• Water soluble 
• Found in fresh fruit [citrus] and 
vegetables 
• Deficiency disease [caused by lack of] – 
scurvy – bleeding gums and poor wound 
healing
19
20
21 
• Vitamin D [caliciferol] 
• Fat soluble 
• Source – dairy products, 
liver, fish oils etc. 
• Made by skin in sunlight 
• Deficiency disease 
• – rickets in children – bowed legs 
• - Osteomalacia in adults 
• Needed to absorb Ca from gut and 
also tooth and bone formation
22 
Minerals 
• Soluble inorganic salts that contain 
essential elements 
• 6 macro major – need in large amounts 
– C, H, O, N, P and S 
• 5 micro small amounts needed 
– Na, K, Ca, Mg and Cl 
• Trace Elements only tiny amounts needed 
(mg) – Fe, Cu and Zn
23 
Examples of required minerals 
Plants 
• Ca – make middle lamella 
• Mg – make chlorophyll 
Animals 
• Ca – bones and teeth 
• Fe – Haemoglobin to transport O2
24 
Roles 
• Present in compounds that make hard 
parts of bodies 
• Present in compounds that form soft 
tissues 
• Maintain correct fluid concentration of 
cell and body fluids
25 
Water 
• Transport medium for blood cells etc. 
• Excellent solvent – good medium for 
chemical processes 
• Strong cohesion [ sticking to itself] and 
adhesion [sticking to other substances] 
– transpiration stream 
• Temperature stable – needs a lot of 
heat to change temperature
26 
• Participant in metabolic reactions 
– Anabolism – removes water 
– Catabolism – adds water 
– Photosynthesis – uses 
– Respiration – produces 
• Participant in movement into and out of 
cells – medium for movement 
• Control of cell shape – osmosis causes 
turgor pressure – guard cell opening 
• Component of cytoplasm
27 
Hormones as regulators of 
metabolic activity 
• Are signal that activate specific 
metabolic programs in their target 
cells by 
a) Influencing transport of materials 
across membranes 
b) Activating or inhibiting enzymes 
c) Activating or inhibiting genes and 
thus influence protein synthesis
28 
Metabolism influenced by 
hormones 
Insulin 
• Uses 
1.Uptake of amino acids and 
glucose by living cells 
2.Protein synthesis 
3.Lipid formation
29 
Balanced Diet 
• One that contains the correct amounts of 
carbohydrates, proteins, fats, vitamins, 
minerals and water. 
• Depends on age, the sex of the individual 
and the activities being undertaken by the 
individual. 
• Teenagers should intake 12000 kJ for 
boys and 9600 kJ for girls per day
30
31
32 
Dieting 
• Most people who go on a diet do so 
because they are overweight. 
• It usually means that the intake and 
variety of food is reduced drastically. 
• This is bad because it does not give a 
balanced diet and the weight lost is 
usually put back on very quickly. 
• To lose weight you must reduce the 
amount of fatty foods and eat more fruit 
and vegetables.
• Undernourished 
• Not enough food 
• At its worst starvation 
• Over 1/3 of the worlds population is 
undernourished 
• Malnourished 
• The wrong proportions of the various food types 
• Too much fat leads to obesity, coronary heart 
disease 
• Too much sugar can lead to diabetes 
33
34 
How to loose weight 
• Reduce fat intake 
• Reduce alcohol intake 
• Increase vegetable, fruit and cereal 
intake 
• Increase exercise
35 
Food Tests
36 
Fats 
Brown Paper Test 
• Rub some fat [butter] on brown paper 
• Rub water on another piece 
• Dry paper 
• If a translucent spot stays its a fat. 
• [translucent means lets light through 
but you can’t see through it]
37 
Starch 
Iodine Test 
• Add some iodine solution 
[brown] to the suspected starch 
e.g. bread and potato. 
• If it goes from brown to 
blue/black then starch is present
38 
Proteins 
• Place sample in test tube [albumen] 
• Add sodium hydroxide solution and mix 
• [caution sodium hydroxide solution is 
caustic - burns skin] 
• add a few drops of copper sulphate 
solution. 
• If it turn from blue to purple/violet then 
protein is present
39 
Reducing Sugars 
• Place some glucose in a test tube 
• Dissolve in water 
• Add Benedict’s Solution [or Fehling’s 
solution A +B and mix] which is blue 
• Place in a hot water bath at <100oC 
for 3 minutes 
• If it turns red/orange then glucose [ a 
Reducing Sugar] present
1. Give 4 functions of food 
2. Test for starch 
3. Elements present in proteins 
4. Test for protein 
5. Two elements with ratio 2:1 in carbohydrates 
6. Test for reducing sugars 
7. Name a water [fat] soluble vitamin, its source 
and a disease caused by a lack of it. 
8. What are these types of diseases called? 
40
9. Test for fats 
10.Give the uses of roughage. 
11.What is a balanced diet? 
12.What does malnourished mean? 
13.What is a fat composed of? 
14.Fat / oil 
15.Saturated – source and difference 
16.Macro, micro and trace elements. 
17.What is a trace element? 
18. two examples of minerals needed by (a) 
plants and (b) animals and what each does 
41
Answers 
1. Give 4 functions of food [4] energy / material 
source / continuity / metabolism 
2. Test for starch [4] add iodine / starch present 
brown / to blue-black / absent stays brown 
3. Elements present in proteins [6] C, H, O, N, S, 
P 
4. Test for protein [3] add biuret reagent / blue to 
lilac if present 
5. Elements in carbohydrates with ratio [4] C,H,O 
/ 2H : 1O 42
6. Test for reducing sugars [4] Benedict’s / heat 
gently / blue / to red pptte 
7. Name a water [fat] soluble vitamin, its source 
and a disease caused by a lack of it. [3] C, 
citrus fruits, scurvy 
8. What are these types of diseases called? [1] 
deficiency 
43
9. Test for fats [3] brown paper / permanent / 
translucent spot 
10.Give the uses of roughage [3] prevent 
constipation / eases digestive transit / absorbs 
water / prevents cancer 
11.What is a balanced diet? [2] All nutrients / in 
correct amounts 
12.What does malnourished mean? [3] Too much 
or too little / of one or more / food types 
13.What is a fat composed of? [3] Glycerol / 3 / 
fatty acids 
44
14.Difference between fat and oil [3] fat solid / 
oil liquid / room conditions 
15.Saturated – source and difference [2] animals 
/ forms cholesterol 
16.List [7] (a) Macro, C H O N S P (b) micro Na K 
Ca Mg and Cl (c) trace elements Fe, Cu, Zn. 
17.What is a trace element? [2] Only needed / in 
tiny amounts 
18. Two examples of minerals needed by [8] (a) 
plants Mg, Chlorophyll Ca Pectate and (b) 
animals Fe Haemoglobin Ca Bones and teeth45

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1 2 nutrition_and_biomolecules v2

  • 1. 1 1.2 Nutrition and Biomolecules Food Chemistry
  • 2. 2 Functions of food 1. Energy source – 90% used to make ATP 2. Materials Source - for growth, repair, defence 3. Continuity – replacement of cells and reproduction 4. Metabolism - maintaining cells at high level of chemical efficiency
  • 3. 3 • Metabolism - all reactions in living body • Anabolism building up of substances – input of energy required • E.g. photosynthesis, DNA replication, RNA formation, muscle building • Catabolism – breaking down of complex molecules – e.g. digestion • Enzymes protein biological catalysts, catalyse reactions [speed up – not used up in] • Respiration – release of energy from food
  • 4. 4 Protein • Chemical elements C, H, O, N also P and S • Biomolecular sub-units – Amino Acids [20] • Linked by peptide bonds • One or more chains • Differ in sequence and number of a.a.’s • Made in ribosomes • Sources meat, fish, eggs, milk, cheese, pulses
  • 5. 5
  • 6. 6 Roles • Structural • Keratin in hair, skin – protects • Myosin in muscle • Metabolic • Enzymes – protein biological catalysts
  • 7. 7 Other roles • Antibodies • Haemoglobin • Chlorophyll • Insulin • Collagen
  • 8. Shapes • Globular or Fibrous • Amino acids join together at angles • so as the protein forms it becomes folded • It can form a long fibre • Or it can become a ball • The function of a protein (enzyme) depends on its shape • If we heat much above 60oC or shake it become denatured i.e. It loses its function 8
  • 9. 9 Carbohydrate Chemical elements C, H, O [ H:O ratio 2:1 ] Monosaccharides [1 sugar unit] Glucose [C6H12O6], fructose, galactose Disaccharides [2 sugar units] Maltose, sucrose [C12H22O11], lactose Polysaccharides [many sugar units] Starch [plants], glycogen [animals]
  • 10. 10 Types of carbohydrates Sugar • Found in honey, jam, sweets , fizzy drinks, alcohol. • [Most processed foods have sugar added] • Provides energy [quickly]
  • 11. 11 Carbohydrates continued Starch • Found in potatoes, flour, bread, pasta, rice, cakes etc. • Provides energy [ more slowly over a longer period] • Test for with iodine which goes from brown to blue/black if starch is present.
  • 12. 12 Carbohydrates continued Fibre [cellulose] • Found in whole-grain cereals [e.g. Weetabix, Shredded Wheat etc.] and vegetables. • Function to retain water and prevent constipation and also cancer of the bowel.
  • 13. 13 Carbohydrate Roles Structural • Cellulose – plant cell walls – glued together to make rigid structure • Chitin – cell walls of fungi, insect cuticles Metabolic • Energy source – respiration of glucose • Energy store – Starch in plants – Glycogen in animals
  • 14. 14 Lipids [fats] • Chemical Elements C, H, O • Glycerol + 3 fatty acids • Fat solid at RTP • Oil liquid at RTP • Lipids rich in saturated fatty acids [found mainly in animal fats] are associated with heart disease • Sources – meat, milk, butter, cheese, olives, maize, sunflower seeds
  • 15. 15 Fatty Acid Glycerol Fatty Acid Fatty Acid Phospholipid Glycerol Fatty Acid Fatty Acid Lipid PO4
  • 16. 16 Roles Structural • Phospholipid forms plasmalemma • Insulates organs e.g.kidney • Cuticle of leaves Metabolic • Storage of excess carbohydrate • Energy by respiration • Hormones – some are lipids • Stores fat soluble vitamins e.g. D
  • 17. 17 Other • Heat Energy store – twice energy of carbohydrates per gram • insulation – sub-cutaneous layer • Protection – pads on feet and hands • Waterproofing – sebum on hair and skin • Storage – adipose tissue
  • 18. 18 Vitamins • Non-protein substance required in tiny amounts for healthy metabolism • Vitamin C [Ascorbic Acid] • Water soluble • Found in fresh fruit [citrus] and vegetables • Deficiency disease [caused by lack of] – scurvy – bleeding gums and poor wound healing
  • 19. 19
  • 20. 20
  • 21. 21 • Vitamin D [caliciferol] • Fat soluble • Source – dairy products, liver, fish oils etc. • Made by skin in sunlight • Deficiency disease • – rickets in children – bowed legs • - Osteomalacia in adults • Needed to absorb Ca from gut and also tooth and bone formation
  • 22. 22 Minerals • Soluble inorganic salts that contain essential elements • 6 macro major – need in large amounts – C, H, O, N, P and S • 5 micro small amounts needed – Na, K, Ca, Mg and Cl • Trace Elements only tiny amounts needed (mg) – Fe, Cu and Zn
  • 23. 23 Examples of required minerals Plants • Ca – make middle lamella • Mg – make chlorophyll Animals • Ca – bones and teeth • Fe – Haemoglobin to transport O2
  • 24. 24 Roles • Present in compounds that make hard parts of bodies • Present in compounds that form soft tissues • Maintain correct fluid concentration of cell and body fluids
  • 25. 25 Water • Transport medium for blood cells etc. • Excellent solvent – good medium for chemical processes • Strong cohesion [ sticking to itself] and adhesion [sticking to other substances] – transpiration stream • Temperature stable – needs a lot of heat to change temperature
  • 26. 26 • Participant in metabolic reactions – Anabolism – removes water – Catabolism – adds water – Photosynthesis – uses – Respiration – produces • Participant in movement into and out of cells – medium for movement • Control of cell shape – osmosis causes turgor pressure – guard cell opening • Component of cytoplasm
  • 27. 27 Hormones as regulators of metabolic activity • Are signal that activate specific metabolic programs in their target cells by a) Influencing transport of materials across membranes b) Activating or inhibiting enzymes c) Activating or inhibiting genes and thus influence protein synthesis
  • 28. 28 Metabolism influenced by hormones Insulin • Uses 1.Uptake of amino acids and glucose by living cells 2.Protein synthesis 3.Lipid formation
  • 29. 29 Balanced Diet • One that contains the correct amounts of carbohydrates, proteins, fats, vitamins, minerals and water. • Depends on age, the sex of the individual and the activities being undertaken by the individual. • Teenagers should intake 12000 kJ for boys and 9600 kJ for girls per day
  • 30. 30
  • 31. 31
  • 32. 32 Dieting • Most people who go on a diet do so because they are overweight. • It usually means that the intake and variety of food is reduced drastically. • This is bad because it does not give a balanced diet and the weight lost is usually put back on very quickly. • To lose weight you must reduce the amount of fatty foods and eat more fruit and vegetables.
  • 33. • Undernourished • Not enough food • At its worst starvation • Over 1/3 of the worlds population is undernourished • Malnourished • The wrong proportions of the various food types • Too much fat leads to obesity, coronary heart disease • Too much sugar can lead to diabetes 33
  • 34. 34 How to loose weight • Reduce fat intake • Reduce alcohol intake • Increase vegetable, fruit and cereal intake • Increase exercise
  • 36. 36 Fats Brown Paper Test • Rub some fat [butter] on brown paper • Rub water on another piece • Dry paper • If a translucent spot stays its a fat. • [translucent means lets light through but you can’t see through it]
  • 37. 37 Starch Iodine Test • Add some iodine solution [brown] to the suspected starch e.g. bread and potato. • If it goes from brown to blue/black then starch is present
  • 38. 38 Proteins • Place sample in test tube [albumen] • Add sodium hydroxide solution and mix • [caution sodium hydroxide solution is caustic - burns skin] • add a few drops of copper sulphate solution. • If it turn from blue to purple/violet then protein is present
  • 39. 39 Reducing Sugars • Place some glucose in a test tube • Dissolve in water • Add Benedict’s Solution [or Fehling’s solution A +B and mix] which is blue • Place in a hot water bath at <100oC for 3 minutes • If it turns red/orange then glucose [ a Reducing Sugar] present
  • 40. 1. Give 4 functions of food 2. Test for starch 3. Elements present in proteins 4. Test for protein 5. Two elements with ratio 2:1 in carbohydrates 6. Test for reducing sugars 7. Name a water [fat] soluble vitamin, its source and a disease caused by a lack of it. 8. What are these types of diseases called? 40
  • 41. 9. Test for fats 10.Give the uses of roughage. 11.What is a balanced diet? 12.What does malnourished mean? 13.What is a fat composed of? 14.Fat / oil 15.Saturated – source and difference 16.Macro, micro and trace elements. 17.What is a trace element? 18. two examples of minerals needed by (a) plants and (b) animals and what each does 41
  • 42. Answers 1. Give 4 functions of food [4] energy / material source / continuity / metabolism 2. Test for starch [4] add iodine / starch present brown / to blue-black / absent stays brown 3. Elements present in proteins [6] C, H, O, N, S, P 4. Test for protein [3] add biuret reagent / blue to lilac if present 5. Elements in carbohydrates with ratio [4] C,H,O / 2H : 1O 42
  • 43. 6. Test for reducing sugars [4] Benedict’s / heat gently / blue / to red pptte 7. Name a water [fat] soluble vitamin, its source and a disease caused by a lack of it. [3] C, citrus fruits, scurvy 8. What are these types of diseases called? [1] deficiency 43
  • 44. 9. Test for fats [3] brown paper / permanent / translucent spot 10.Give the uses of roughage [3] prevent constipation / eases digestive transit / absorbs water / prevents cancer 11.What is a balanced diet? [2] All nutrients / in correct amounts 12.What does malnourished mean? [3] Too much or too little / of one or more / food types 13.What is a fat composed of? [3] Glycerol / 3 / fatty acids 44
  • 45. 14.Difference between fat and oil [3] fat solid / oil liquid / room conditions 15.Saturated – source and difference [2] animals / forms cholesterol 16.List [7] (a) Macro, C H O N S P (b) micro Na K Ca Mg and Cl (c) trace elements Fe, Cu, Zn. 17.What is a trace element? [2] Only needed / in tiny amounts 18. Two examples of minerals needed by [8] (a) plants Mg, Chlorophyll Ca Pectate and (b) animals Fe Haemoglobin Ca Bones and teeth45