056 CHROMATOGRAPHY 
Chromatography is a method of separation of substances based on 
their different mobility in a given stationary support. 
MATERIALS REQUIRED: 
Black, red and blue ink 
Chalk piece (used for writing on Blackboard) 
A lid about 6 cm diameter from a bottle or jar 
A glass tumbler 
A strip of blotting paper or filter paper 
A stick from broom or a small twig
Ink spot 
water 
chalk 
Mix a few drops of black, red and blue ink. 
Place a few drops of this ink on a chalk about 5mm from the 
thick end. Dry the chalk in air and place in the lid containing 
water.
The ink spot should not be in direct contact with the 
water. After some time water rises up the chalk and 
the different colours are separated as bands
Take a strip of blotting paper and place a small drop of the mixed ink on it 
about 1cm from the end. Dip the strip in water and fold and rest the other end 
on a stick on the glass. Water level should be below the ink dot 
As the water rises on the paper, the colours of the ink mixture are dispersed 
as distinct bands

056 chromatography

  • 1.
    056 CHROMATOGRAPHY Chromatographyis a method of separation of substances based on their different mobility in a given stationary support. MATERIALS REQUIRED: Black, red and blue ink Chalk piece (used for writing on Blackboard) A lid about 6 cm diameter from a bottle or jar A glass tumbler A strip of blotting paper or filter paper A stick from broom or a small twig
  • 2.
    Ink spot water chalk Mix a few drops of black, red and blue ink. Place a few drops of this ink on a chalk about 5mm from the thick end. Dry the chalk in air and place in the lid containing water.
  • 3.
    The ink spotshould not be in direct contact with the water. After some time water rises up the chalk and the different colours are separated as bands
  • 4.
    Take a stripof blotting paper and place a small drop of the mixed ink on it about 1cm from the end. Dip the strip in water and fold and rest the other end on a stick on the glass. Water level should be below the ink dot As the water rises on the paper, the colours of the ink mixture are dispersed as distinct bands