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Seminar -I
Recent advances in seed
technological aspects of biofuel
plant species.
By
Annaiah Gollar
Pgs14agr6546
• Introduction
• Biofuel crops
• Recent advances in biofuel crop
production
• Case studies
• Conclusion
Content
3
• BIOFUELS are liquid or gaseous fuels
produced from biomass resources and used in
place of, or in addition to, diesel, petrol or
other fossil fuels for transport, stationary,
portable and other applications.
• The term biofuel is used here to mean any
liquid fuel made from plant material.
• A biofuel is a fuel that is produced through
contemporary biological processes.
• Biofuels can be derived directly from plants, or
indirectly from agricultural, commercial, domestic,
and/or industrial wastes.
WHAT ARE BIOFUELS?
4
• Commercial utilization of tree borne oilseeds as
biofuel resources is one of the urgent requirements to
cope up with the shortage of fuel.
• Biofuels are drawing increasing attention
worldwide.
• Biofuels include relatively familiar ones, such as
ethanol made from sugar cane or diesel-like fuel
made from soybean oil, to less familiar fuels crops.
WHAT ARE BIOFUELS?
5
• CATEGORIES
• First generation biofuels (Bioalcohols,
Biodiesel, Vegetable oil, Bioethers, Biogas)
• Second generation biofuels (advanced
biofuels like biohydrogen, biomethanol)
• Third generation biofuels (micro-
organisms like algae)
WHAT ARE BIOFUELS?
6
WHY BIOFUELS?
• SUSTAINABILITY
• POLLUTION THREAT
• REDUCTION OF GREEN HOUSE GAS EMISSIONS
• REGIONAL (RURAL) DEVELOPMENT
• SOCIAL STRUCTURE & AGRICULTURE
IMPORTANCE OF BIODIESEL
• Environment friendly
• Clean burning
• Renewable fuel
• No engine modification
• Increase in engine life
• Biodegradable and non-toxic
• Easy to handle and store
Biofuel production(1000 barrels /day)
Country 2008 2009 2010 2011 2012
North
America
666.479 769.693 916.244 1004.719 975.958
India 5.200 3.016 2.413 8.048 7.238
World wide 1477.321 1634.905 1886.392 1951.800 1901.348
Biofuel crops
• Jatropha (Jatropha curcas)
• Pongamia (Pongamia pinnata)
• Simarouba (Simarouba glauca)
• Mahua (Mhua indica)
• Neem (Azadirachta indica)
• And other oil seed crops
Jatropha (Jatropha curcas)
• Jatropha curcas is considered to have
originated in Latin America,.
• It belongs to family Euphorbiaceae.
• Jatropha is one of the important biofuel crop.
• It is reported to grow in marginal areas.
• ‘jatropha seed oil’is very important.
• Jatropha oil is an important emerging, low-
cost and smokeless alternative of petrol, diesel
• For reducing the dependence on crude oil and
to achieve energy independence by the year
2012, jatropha has been promoted under the
National Biodiesel Mission in India.
Pongamia (Pongamia pinnata)
• It is also known as Karanj
• It belongs to family Fabaceae
• It is native to humid and subtropical
environments,
• Karanj can grow on most soil types ranging
from stony to sandy to clay
• Seeds of Pongamia have about 25-30 per cent
oil
• Most of the physical and chemical properties
of the oil is almost similar to those of the
diesel
• Pongamia oil is also used as a fuel for cooking
and lamps, as a lubricant, water-paint binder,
pesticide, etc...
Simarouba (Simarouba glauca)
• It is native to Central America.
• It belongs to family Simaroubaceae.
• It is evergreen multipurpose tree.
• It grows well even on marginal land under
water stress .
• This tree was first introduced to india by
NBPGR in Amaravathi (MH) research station
in 1966 and UAS Banglore in 1986.
• It is mainly propagated through seed .
• Seed have short viability of 2-3 months.
• Fresh seeds have germination about 60%.
Mahua (Mahua indica)
• It is commonly known as mahua (Hippe).
• It belongs to family sapotaceae.
• It is medium size large size deciduous tree.
• It grows well under marginal soils.
• It is drought tolerant and frost hardy plant.
• It is normally propagated through seeds
• Seeds are used for manufacture of biofuel ,
washing soaps , lubricants etc…
Neem(Azadirachta indica)
• It belongs to family Meliaceae.
• It is typically grown in tropical and
semitropical region.
• It is noted for its drought tolerance.
• It is medicinally important crop.
• Neem seed oil is used in manufacture of
medicines and biofuels.
Oil content of biofuel crops .
Plant name Botanical name Oil content %
Jatropa Jatropa curcas 25-30
Pongamia Pongamia pinnata 27-30
Neem Azadirachta indica 20-25
Simarouba Simarouba gluaca 60-75
Mahua Mahua indica 30-35
General seed characters
• Almost biofuels crop seeds are recalcitrant in
nature .
• Seeds are medium to big size.
• Almost seeds have physical dormancy.
• These seeds are mostly affected by rancidity.
Constraints of biofuel crop
• No private sectors involved in production of
seedling
• In fact limited public sector institution involved
• Lack of unavailability of elite seedlings .
• No proper standard method of seed sowing
• No proper seed treatment
• Poor nursery management
• Less interest in micropropagation
• Confusion about harvest indices
• Storage problem
• Seed orientation
• Seed treatment
• Nursery management
• Management aspects
• Harvest index
• Micro propagation
Advances in seed aspects
22
Seed orientation
• Seed orientation
It is one such technique where the seeds are
sown in respect to genetic structural
component viz...embryo and storage tissue.
• It has effective influence on production of elite
seedlings.
• Their is need for standard orientation of
biofuel seeds for elite seedling production.
Study on Seed Orientation for Production Quality Seedlings in
Bio fuel Tree Species
Mariappan, et al.2014
Study on Seed Orientation for Production Quality Seedlings in
Bio fuel Tree Species
Mariappan, et al.2014,
Study on Seed Orientation for Production Quality Seedlings in
Bio fuel Tree Species
Mariappan, et al.2014
m
Study on Seed Orientation for Production Quality Seedlings in
Bio fuel Tree Species
Mariappan, et al.2014
m
Study on Seed Orientation for Production Quality Seedlings in
Bio fuel Tree Species
Mariappan, et al.2014
m
Seed treatment
• Dormancy breaking mechanism
• Seed pelleting .
• Seed priming.
• Seed hardening.
EFFECT OF PLANT GROWTH REGULATORS ON SEED
GERMINATION OF OIL YIELDING PLANT SIMAROUBA GLAUCA .
Patil and Gaikwad.2011
Influence of dormancy breaking
mechanisms on simarouba seeds
(Simarouba glauca DC.)
Komala et al. 2015
Influence of dormancy breaking mechanisms on simarouba
seeds (Simarouba glauca DC.)
Treatments Germination %
T1 Control (with seed coat) 77.75
T2 Complete seed coat removal 41.75
T3 Seeds are hammered with wooden
piece to break seed coat
42.00
T4 Dipping of the seeds in hot water of
600C for 15 sec.
62.75
T5 Dipping the seeds in conc. H2SO4 for
15 sec.
66.50
T6 Rubbing the seeds with sand paper 63.50
T7Seeds are oven dried at 400 C for hours 20.00
T8 Seeds are tied in cloth bag and
watered regularly to keep them wet.
57.75
Mean 54
CD at 5% 1.42
Effect of organic seed pelleting on seed storability and
quality seedling production in biofuel tree species
Influence of botanical seed pelleting on storability of Jatropha seeds
Srimathi et al.2013
Effect of organic seed pelleting on seed storability and
quality seedling production in biofuel tree species
Influence of botanical seed pelleting on storability of pungamia seeds.
Srimathi et al.2013
Effect of liquid biofertilizers on enhancement of germination in stored
seeds of Pongamia pinnata
Influence of liquid biofertilizers on stored seeds of Pongamia pinnata
Mariappan et al.2014
T1 (Azospirillum 0.5%), T2 (Azospirillum 1.0%), T3 (Azospirillum 1.5%),
T4 (Phosphobacterium 0.5%), T5 (Phosphobacterium 1.0%) and
T6 (Phosphobacterium 1.5%).
Nursery management
• Poor establishment of seedlings
• Uneven and unpredictable fruiting of trees
ex ,Pongamia
• There is a phenomenon known as dormancy
• Inadequate availability of quality planting material
• Almost seeds are recalcitrant in nature.
Effect of growing media on seed germination and vigour in biofuel tree
species
Effect of media on germination behaviour of Jatropha curcas
Mariappan et al.2014
Effect of growing media on seed germination and vigour in biofuel tree
species
Effect of media on germination behaviour of Pongamia pinnata
Mariappan et al.2014
Influence of various potting mixtures
on germination and root length of tree
species in vertisols of northern
Karnataka
Devaranavadgi et al., (2010)
Devaranavadgi et al., 2010
Influence of nursery mixture compositions on simarouba seeds
(Simarouba glauca )
Komala et al.2015
Harvest index
• Fruit colour is considered as an easily
identifiable visual index of seed maturation
• Standardization of fruit colour is very
important for biofuel crops .
• It would helps seed collector to collect ground
quality seeds.
Evaluation of fruit colour as harvest index for selection of
quality seeds in biofuel crops
Fresh fruit colour on Pongamia pinnata fruit morphology, seed and seedling quality
characters.
Srimathi et al. 2013
Evaluation of fruit colour as harvest index for selection of
quality seeds in biofuel crops
Fresh fruit colour on Simarouba glauca fruit morphology, seed and seedling quality
characters.
Srimathi et al. 2013
Evaluation of fruit colour as harvest index for selection of
quality seeds in biofuel crops
Fresh fruit colour on Azardirachta indica fruit morphology, seed and seedling
quality characters .
Srimathi et al. 2013
Evaluation of fruit colour as harvest index for selection of
quality seeds in biofuel crops
Fresh fruit colour on Madhuca longifolia fruit morphology, seed and seedling
quality characters .
Srimathi et al. 2013
Physiological Changes in the Seeds of Jatropha curcas . at
Different Stages of Fruit Maturity
Seed germination, moisture content, total sugars, reducing sugars, non-reducing
sugars, total phenols and oil content in the seeds at different stages of capsule
maturity in J. Curcas
Suheel and Sheikh 2015
Micro propagation
• All biofuels are conventially propagated through seeds .
• Major constrain is viability of seed is less .
• Plants produced at nursery are polygamodioecious
nature.
• Grafting is recommended but it is labour intensive.
• To exploit oil at max. Extent it is necessary to select
best genotype.
• To meet out demand fuel in the near future.
IN VITRO REGENERATION FROM SHOOT TIP AND NODAL EXPLANTS OF
SIMAROUBA GLAUCA , A PROMISING BIODIESEL TREE
Effect of BAP and NAA on multiple shoot induction from shoot tip and nodal
explants of S.glauca
Shukla and Padmaja 2013
IN VITRO REGENERATION FROM SHOOT TIP AND NODAL EXPLANTS OF
SIMAROUBA GLAUCA , A PROMISING BIODIESEL TREE
Effect of different concentrations of auxins on rooting of in vitro-raised shoots in
S.glauca after 4 weeks in culture.
• 4 weeks in culture
Shukla and Padmaja 2013
In vitro clonal propagation of physic nut (Jatropha curcas )
Effect of different concentration of BA , NAA and IBA in MS medium on
multiple shoot regeneration through axillary nodes of Jatropha curcas
Shrivastava and Banerjee 2008
In vitro clonal propagation of physic nut (Jatropha curcas )
Effect of NAA and IBA in ½ MS Media on in vitro Rooting of Shoots and %
survival rates in the field
Shrivastava and Banerjee2008
Management aspects
• Monoculture
• Inter cultivation
• Hedge cropping
Relationships among Jatropha curcas seed yield and
vegetative plant components under different
management and cropping systems in Indonesia
Juliana et al.2015
Relationships among Jatropha curcas seed yield and
vegetative plant components under different
management and cropping systems in Indonesia
Juliana et al.2015
Mean seed yield of jatropha shrubs in intercropping system
with combination of root barrier and mulch treatments
Relationships among Jatropha curcas seed yield and
vegetative plant components under different
management and cropping systems in Indonesia
Juliana et al.2015
Mean seed yield and plant components of jatropha shrubs in hedge system with
combination of plant spacing of 10 cm or 30 cm and pruning treatment.
Conclusion
ííííí
THANK YOU 62

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Bio Annaiah.pptx

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  • 2. Seminar -I Recent advances in seed technological aspects of biofuel plant species. By Annaiah Gollar Pgs14agr6546
  • 3. • Introduction • Biofuel crops • Recent advances in biofuel crop production • Case studies • Conclusion Content 3
  • 4. • BIOFUELS are liquid or gaseous fuels produced from biomass resources and used in place of, or in addition to, diesel, petrol or other fossil fuels for transport, stationary, portable and other applications. • The term biofuel is used here to mean any liquid fuel made from plant material. • A biofuel is a fuel that is produced through contemporary biological processes. • Biofuels can be derived directly from plants, or indirectly from agricultural, commercial, domestic, and/or industrial wastes. WHAT ARE BIOFUELS? 4
  • 5. • Commercial utilization of tree borne oilseeds as biofuel resources is one of the urgent requirements to cope up with the shortage of fuel. • Biofuels are drawing increasing attention worldwide. • Biofuels include relatively familiar ones, such as ethanol made from sugar cane or diesel-like fuel made from soybean oil, to less familiar fuels crops. WHAT ARE BIOFUELS? 5
  • 6. • CATEGORIES • First generation biofuels (Bioalcohols, Biodiesel, Vegetable oil, Bioethers, Biogas) • Second generation biofuels (advanced biofuels like biohydrogen, biomethanol) • Third generation biofuels (micro- organisms like algae) WHAT ARE BIOFUELS? 6
  • 7. WHY BIOFUELS? • SUSTAINABILITY • POLLUTION THREAT • REDUCTION OF GREEN HOUSE GAS EMISSIONS • REGIONAL (RURAL) DEVELOPMENT • SOCIAL STRUCTURE & AGRICULTURE
  • 8. IMPORTANCE OF BIODIESEL • Environment friendly • Clean burning • Renewable fuel • No engine modification • Increase in engine life • Biodegradable and non-toxic • Easy to handle and store
  • 9. Biofuel production(1000 barrels /day) Country 2008 2009 2010 2011 2012 North America 666.479 769.693 916.244 1004.719 975.958 India 5.200 3.016 2.413 8.048 7.238 World wide 1477.321 1634.905 1886.392 1951.800 1901.348
  • 10. Biofuel crops • Jatropha (Jatropha curcas) • Pongamia (Pongamia pinnata) • Simarouba (Simarouba glauca) • Mahua (Mhua indica) • Neem (Azadirachta indica) • And other oil seed crops
  • 11. Jatropha (Jatropha curcas) • Jatropha curcas is considered to have originated in Latin America,. • It belongs to family Euphorbiaceae. • Jatropha is one of the important biofuel crop. • It is reported to grow in marginal areas. • ‘jatropha seed oil’is very important.
  • 12. • Jatropha oil is an important emerging, low- cost and smokeless alternative of petrol, diesel • For reducing the dependence on crude oil and to achieve energy independence by the year 2012, jatropha has been promoted under the National Biodiesel Mission in India.
  • 13. Pongamia (Pongamia pinnata) • It is also known as Karanj • It belongs to family Fabaceae • It is native to humid and subtropical environments, • Karanj can grow on most soil types ranging from stony to sandy to clay
  • 14. • Seeds of Pongamia have about 25-30 per cent oil • Most of the physical and chemical properties of the oil is almost similar to those of the diesel • Pongamia oil is also used as a fuel for cooking and lamps, as a lubricant, water-paint binder, pesticide, etc...
  • 15. Simarouba (Simarouba glauca) • It is native to Central America. • It belongs to family Simaroubaceae. • It is evergreen multipurpose tree. • It grows well even on marginal land under water stress . • This tree was first introduced to india by NBPGR in Amaravathi (MH) research station in 1966 and UAS Banglore in 1986.
  • 16. • It is mainly propagated through seed . • Seed have short viability of 2-3 months. • Fresh seeds have germination about 60%.
  • 17. Mahua (Mahua indica) • It is commonly known as mahua (Hippe). • It belongs to family sapotaceae. • It is medium size large size deciduous tree. • It grows well under marginal soils. • It is drought tolerant and frost hardy plant. • It is normally propagated through seeds • Seeds are used for manufacture of biofuel , washing soaps , lubricants etc…
  • 18. Neem(Azadirachta indica) • It belongs to family Meliaceae. • It is typically grown in tropical and semitropical region. • It is noted for its drought tolerance. • It is medicinally important crop. • Neem seed oil is used in manufacture of medicines and biofuels.
  • 19. Oil content of biofuel crops . Plant name Botanical name Oil content % Jatropa Jatropa curcas 25-30 Pongamia Pongamia pinnata 27-30 Neem Azadirachta indica 20-25 Simarouba Simarouba gluaca 60-75 Mahua Mahua indica 30-35
  • 20. General seed characters • Almost biofuels crop seeds are recalcitrant in nature . • Seeds are medium to big size. • Almost seeds have physical dormancy. • These seeds are mostly affected by rancidity.
  • 21. Constraints of biofuel crop • No private sectors involved in production of seedling • In fact limited public sector institution involved • Lack of unavailability of elite seedlings . • No proper standard method of seed sowing • No proper seed treatment • Poor nursery management • Less interest in micropropagation • Confusion about harvest indices • Storage problem
  • 22. • Seed orientation • Seed treatment • Nursery management • Management aspects • Harvest index • Micro propagation Advances in seed aspects 22
  • 23. Seed orientation • Seed orientation It is one such technique where the seeds are sown in respect to genetic structural component viz...embryo and storage tissue. • It has effective influence on production of elite seedlings. • Their is need for standard orientation of biofuel seeds for elite seedling production.
  • 24. Study on Seed Orientation for Production Quality Seedlings in Bio fuel Tree Species Mariappan, et al.2014
  • 25. Study on Seed Orientation for Production Quality Seedlings in Bio fuel Tree Species Mariappan, et al.2014,
  • 26. Study on Seed Orientation for Production Quality Seedlings in Bio fuel Tree Species Mariappan, et al.2014 m
  • 27. Study on Seed Orientation for Production Quality Seedlings in Bio fuel Tree Species Mariappan, et al.2014 m
  • 28. Study on Seed Orientation for Production Quality Seedlings in Bio fuel Tree Species Mariappan, et al.2014 m
  • 29. Seed treatment • Dormancy breaking mechanism • Seed pelleting . • Seed priming. • Seed hardening.
  • 30. EFFECT OF PLANT GROWTH REGULATORS ON SEED GERMINATION OF OIL YIELDING PLANT SIMAROUBA GLAUCA . Patil and Gaikwad.2011
  • 31. Influence of dormancy breaking mechanisms on simarouba seeds (Simarouba glauca DC.) Komala et al. 2015
  • 32. Influence of dormancy breaking mechanisms on simarouba seeds (Simarouba glauca DC.) Treatments Germination % T1 Control (with seed coat) 77.75 T2 Complete seed coat removal 41.75 T3 Seeds are hammered with wooden piece to break seed coat 42.00 T4 Dipping of the seeds in hot water of 600C for 15 sec. 62.75 T5 Dipping the seeds in conc. H2SO4 for 15 sec. 66.50 T6 Rubbing the seeds with sand paper 63.50 T7Seeds are oven dried at 400 C for hours 20.00 T8 Seeds are tied in cloth bag and watered regularly to keep them wet. 57.75 Mean 54 CD at 5% 1.42
  • 33. Effect of organic seed pelleting on seed storability and quality seedling production in biofuel tree species Influence of botanical seed pelleting on storability of Jatropha seeds Srimathi et al.2013
  • 34. Effect of organic seed pelleting on seed storability and quality seedling production in biofuel tree species Influence of botanical seed pelleting on storability of pungamia seeds. Srimathi et al.2013
  • 35. Effect of liquid biofertilizers on enhancement of germination in stored seeds of Pongamia pinnata Influence of liquid biofertilizers on stored seeds of Pongamia pinnata Mariappan et al.2014 T1 (Azospirillum 0.5%), T2 (Azospirillum 1.0%), T3 (Azospirillum 1.5%), T4 (Phosphobacterium 0.5%), T5 (Phosphobacterium 1.0%) and T6 (Phosphobacterium 1.5%).
  • 36. Nursery management • Poor establishment of seedlings • Uneven and unpredictable fruiting of trees ex ,Pongamia • There is a phenomenon known as dormancy • Inadequate availability of quality planting material • Almost seeds are recalcitrant in nature.
  • 37. Effect of growing media on seed germination and vigour in biofuel tree species Effect of media on germination behaviour of Jatropha curcas Mariappan et al.2014
  • 38. Effect of growing media on seed germination and vigour in biofuel tree species Effect of media on germination behaviour of Pongamia pinnata Mariappan et al.2014
  • 39. Influence of various potting mixtures on germination and root length of tree species in vertisols of northern Karnataka Devaranavadgi et al., (2010)
  • 41. Influence of nursery mixture compositions on simarouba seeds (Simarouba glauca ) Komala et al.2015
  • 42. Harvest index • Fruit colour is considered as an easily identifiable visual index of seed maturation • Standardization of fruit colour is very important for biofuel crops . • It would helps seed collector to collect ground quality seeds.
  • 43. Evaluation of fruit colour as harvest index for selection of quality seeds in biofuel crops Fresh fruit colour on Pongamia pinnata fruit morphology, seed and seedling quality characters. Srimathi et al. 2013
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  • 45. Evaluation of fruit colour as harvest index for selection of quality seeds in biofuel crops Fresh fruit colour on Simarouba glauca fruit morphology, seed and seedling quality characters. Srimathi et al. 2013
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  • 47. Evaluation of fruit colour as harvest index for selection of quality seeds in biofuel crops Fresh fruit colour on Azardirachta indica fruit morphology, seed and seedling quality characters . Srimathi et al. 2013
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  • 49. Evaluation of fruit colour as harvest index for selection of quality seeds in biofuel crops Fresh fruit colour on Madhuca longifolia fruit morphology, seed and seedling quality characters . Srimathi et al. 2013
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  • 51. Physiological Changes in the Seeds of Jatropha curcas . at Different Stages of Fruit Maturity Seed germination, moisture content, total sugars, reducing sugars, non-reducing sugars, total phenols and oil content in the seeds at different stages of capsule maturity in J. Curcas Suheel and Sheikh 2015
  • 52. Micro propagation • All biofuels are conventially propagated through seeds . • Major constrain is viability of seed is less . • Plants produced at nursery are polygamodioecious nature. • Grafting is recommended but it is labour intensive. • To exploit oil at max. Extent it is necessary to select best genotype. • To meet out demand fuel in the near future.
  • 53. IN VITRO REGENERATION FROM SHOOT TIP AND NODAL EXPLANTS OF SIMAROUBA GLAUCA , A PROMISING BIODIESEL TREE Effect of BAP and NAA on multiple shoot induction from shoot tip and nodal explants of S.glauca Shukla and Padmaja 2013
  • 54. IN VITRO REGENERATION FROM SHOOT TIP AND NODAL EXPLANTS OF SIMAROUBA GLAUCA , A PROMISING BIODIESEL TREE Effect of different concentrations of auxins on rooting of in vitro-raised shoots in S.glauca after 4 weeks in culture. • 4 weeks in culture Shukla and Padmaja 2013
  • 55. In vitro clonal propagation of physic nut (Jatropha curcas ) Effect of different concentration of BA , NAA and IBA in MS medium on multiple shoot regeneration through axillary nodes of Jatropha curcas Shrivastava and Banerjee 2008
  • 56. In vitro clonal propagation of physic nut (Jatropha curcas ) Effect of NAA and IBA in ½ MS Media on in vitro Rooting of Shoots and % survival rates in the field Shrivastava and Banerjee2008
  • 57. Management aspects • Monoculture • Inter cultivation • Hedge cropping
  • 58. Relationships among Jatropha curcas seed yield and vegetative plant components under different management and cropping systems in Indonesia Juliana et al.2015
  • 59. Relationships among Jatropha curcas seed yield and vegetative plant components under different management and cropping systems in Indonesia Juliana et al.2015 Mean seed yield of jatropha shrubs in intercropping system with combination of root barrier and mulch treatments
  • 60. Relationships among Jatropha curcas seed yield and vegetative plant components under different management and cropping systems in Indonesia Juliana et al.2015 Mean seed yield and plant components of jatropha shrubs in hedge system with combination of plant spacing of 10 cm or 30 cm and pruning treatment.