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International Journal of Trend in Scientific Research and Development (IJTSRD)
Volume 7 Issue 2, March-April 2023 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470
@ IJTSRD | Unique Paper ID – IJTSRD53877 | Volume – 7 | Issue – 2 | March-April 2023 Page 35
Development of Sorghum Noodles Fortification with
Moringa (Moringa Oleifera) Leaf Powder
Muskan Bhavesh Patel1
, Dhanya Joseph2
1
MSc Student, Department of Food Technology, 2
Assistant Professor, Department of Food Technology,
1,2
Parul Institute of Applied Science, Parul University, Waghodia, Vadodara, Gujarat, India
ABSTRACT
The study of noodles from sorghum flour, rice flour, and, Moringa
oleifera leaf powder development was carried out at the Department
of Food Technology, Parul Institute of Applied Sciences, Parul
University, Vadodara Gujarat, India. The cereal grain sorghum has a
number of agronomic benefits, contains substances that are good for
your health, and is gluten-free. One of the most beneficial tropical
trees is Moringa Oleifera, which offers vital nutrients and
antioxidants to treat vitamin and mineral deficiencies, support a
healthy cardiovascular system, help to promote normal blood sugar
levels, neutralize free radicals, improve eyesight, mental clarity, and
bone strength, and may be helpful in cases of malnutrition, general
weakness, lactating mothers, menopause, depression, and
osteoporosis. Moringa is a multi-beneficial plant with high nutritional
value, making it an excellent protein supplement for malnutrition and
animal feed. Although Moringa oleifera leaves are high in nutrients,
the addition of saponins may increase the bitter taste and unpleasant
smell.
This study set out to create noodles using moringa leaf powder,
sorghum flour, and rice flour and to assess their sensory acceptance,
antinutritional content and proximate composition. The findings
revealed in moisture (7.62), total ash (5.48), protein (6.80), fat (1.86),
carbohydrate (78.24), energy (358.9), calcium (242.4) (Ca), and
potassium (353.5) content, the total number of E. coli 0157, and coli
form. In order to guarantee global food security and meet nutritional
needs, the concentration of moringa plant powder must be limited.
Moringa Oleifera leaves powder affected, macronutrient, and
minerals content in wet noodle products. It is recommended that
concentrations of 5%, 10%, and 15% should not exceed 5% because
it affects the organoleptic of the product.
How to cite this paper: Muskan
Bhavesh Patel | Dhanya Joseph
"Development of Sorghum Noodles
Fortification with Moringa (Moringa
Oleifera) Leaf
Powder" Published
in International
Journal of Trend in
Scientific Research
and Development
(ijtsrd), ISSN: 2456-
6470, Volume-7 |
Issue-2, April 2023, pp.35-40, URL:
www.ijtsrd.com/papers/ijtsrd53877.pdf
Copyright © 2023 by author (s) and
International Journal of Trend in
Scientific Research and Development
Journal. This is an
Open Access article
distributed under the
terms of the Creative Commons
Attribution License (CC BY 4.0)
(http://creativecommons.org/licenses/by/4.0)
KEYWORDS: noodles, sorghum
(jowar), Moringa oleifera (drumstick
tree) leaf powder
1. INTRODUCTION
Noodles are a widely consumed food item in many
parts of the world. In many Asian nations, noodles
have been considered as a staple food. In general, the
benefit of instant noodles is that they are simple to
prepare, delicious, and convenient to serve. It also has
a lower cost and a longer shelf life (Ebtihal et al.,
2019). Noodles can be made from a variety of flour
such as rice, wheat, maize, finger millet (ragi),
sorghum (jowar), and semolina using an extruder by
adding water and flavorings such as egg and spice.
Noodle consumption has been increasing, but the low
fat and protein content limits its nutritional value. To
improve the overall nutritional profile of noodles we,
use sorghum flour, rice flour, and Moringa oleifera
leaf powder.
The sorghum crop is a vital food-security cereal crop
in many places of Africa, Asia, and the semi-arid
tropics around the world (FAO/WHO, 2007). In terms
of production, Sorghum is the African continent's
second most important cereal. This is because
Africa's average sorghum production is around 20
million tonnes mt per year (FAO, 2019; Taylor, 2003;
Collar, 2019). As a gluten-free grain, sorghum lacks
viscoelastic properties. Protein (kafirin) derived from
sorghum is more challenging to hydrate than wheat
gluten protein. Sorghum increased starch content led
IJTSRD53877
International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470
@ IJTSRD | Unique Paper ID – IJTSRD53877 | Volume – 7 | Issue – 2 | March-April 2023 Page 36
to a high gelatinization temperature for starch and a
low water-holding capacity Gluten protein is often
required in fine-dried noodles to strengthen the
noodle's resistance to breaking during the drying
process (Arendt and Zannini, 2013). Sorghum's
nutritional value is distinguished by its protein level,
which ranges from 7 to 16% with an average of 11%
(Taylor and Anyango, 2011). Cysteine was found in
sorghum protein (Kafirin). When sorghum is cooked -
wet, kafirin cross-linking via disulfide bonding
occurs. As a result, sorghum grain protein is difficult
to digest (Duodu et al., 2003).
Moringa oleifera, also known as the drumstick tree, is
a Moringaceae family member and is also known as
the "Miracle Tree." (Mahato et al, 2022). Being a
native of north-western India, it has received
widespread recognition in ethnomedicine as well as
the Ayurvedic and Unani medical systems. (Milla et
al, 2021). According to Saifu (2015), the moringa tree
leaf is used as a famine food to ensure food security.
The moringa tree leaf, according to the NRC, contains
essential nutrients such as protein, amino acids,
carbohydrates, vitamins, minerals, and organic acid
.(Oduro et al. and Madukwe (2013) According to
reports, the moringa tree leaf also has antibacterial
and anti-inflammatory properties. Product
development using moringa leaf powder has not yet
been properly customized and commercialized in
developing countries such as Ethiopia, specifically
Hawassa, despite the fact that moringa provides
multiple benefits such as alleviating malnutrition and
food insecurity, which are common problems in
developing countries.
Moringa has a wide range of nutritional advantages
that are determined by factors like genetic make-up,
environmental factors, and manufacturing techniques.
For an animal to establish immunity(for the synthesis
of antibodies and cells), proteins, minerals (zinc,
copper, and iron), and vitamins (A and E) are also
required. In order to combat infections, these
nutrients also assist organs and tissues in
communicating with one another. Moringa oleifera is
recognized as a versatile plant because of its range of
applications. Excellent sources of calcium, iron, and
vitamins A, B, and C can be found in its leaves.
The leaves of the Moringa plant are said to be
particularly nutrient-dense since they have quantities
of vitamin A, vitamin C, iron, calcium, and potassium
that are comparable to those found in carrot, orange,
spinach, and banana. Based on a comparison of its
amino acid profiles with the FAO/WHO/UNO
reference protein for children, it is likewise a good
source of protein. High antioxidant activity levels
found in moringa leaves have been linked to cancer
chemoprevention, protein oxidation-reduction, and
lipid peroxidation inhibition.
The main purpose of this study is to assess the
acceptability of noodles made with 70% sorghum
flour, 10% rice flour, and 5% moringa (Moringa
oleifera) leaf powder as well as other ingredients. The
assessment was carried out among various samples of
noodles (T0, T1, and T2) containing different
amounts of Moringa oleifera leaf powder (5%, 10%,
and 15%). To complete this noodles development
experiment, chemical test, organoleptic test, mineral
test, and microbiological test are.
2. Materials and Methods:
2.1. Materials:
Sorghum flour, rice flour, and salt were purchased
from the local market in Vadodara, Gujarat, India.
Garlic and Ginger were purchased from the local
market in Vadodara, Gujarat, India. After that garlic
powder and ginger powder prepare in the Parul
Institute of Applied Science Laboratory. however
Moringa oleifera leaf powder was purchased from
Amazon. Sorghum flour, rice flour, and moringa leaf
powder were sieved into fine flour of uniform
particles. The sorghum flour, rice flour, and moringa
powder were later packed in a polyethylene bag and
finally stored at room temperature. The sorghum
flour, rice flour, and moringa leaf powder were then
mixed according to the formulation.
2.2. Methods:
Sorghum Noodle Preparation
Noodles were prepared by using 70% sorghum flour,
and 10% rice flour, with different proportions of
Moringa oleifera leaf powder (5%, 10%, and 15%).
Here, after samples are referred to as T0, T1, and T2
respectively. in the prepared sample T0 ,70%
sorghum flour is used. Initially, for T1 and T2
samples rice flour and sorghum flour were mixed. In
a large mixing bowl, combine the rice flour, sorghum
flour, moringa leaf powder, ginger powder and garlic
powder, and salt. Mix it well and slowly add the
water, a little at a time, and mix the ingredients with
your hands until you get dough that is smooth and
elastic. If the dough is too dry, add a little more water.
If it is too wet, add a little more flour. Wrap the
dough in plastic wrap and let it rest for 15 minutes.
Cut the dough into small pieces that will fit into the
hand extruder. Place the dough into the hand extruder
and turn the handle to push the dough through the
extruder. The dough will come out in the shape of
noodles. Finally, the extruded product (noodles) was
cut into comparable sizes. Than it dried, packed, and
stored at room temperature until cooked and served
for sensory evaluation.
International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470
@ IJTSRD | Unique Paper ID – IJTSRD53877 | Volume – 7 | Issue – 2 | March-April 2023 Page 37
Sorghum flour (70%) + rice flour (10%)
Mixing all raw materials together
(Ginger powder + garlic powder + Moringa oleifera
leaf powder + salt)
Dough making (flour + oil +water)
Dough resting (15 minutes)
Cut the dough into small pieces
Extrusion (hand extruder)
Drying (sun dry-15 hours)
Packaging of noodles
2.3. Flow chart for the processing of noodles
Formulation of sorghum noodles:
Ingredients T0 T1 T2
Sorghum flour 70g 60g 60g
Rice flour 10g 17g 12g
Moringa leaf powder 5g 10g 15g
Ginger powder 5g 5g 5g
Garlic powder 5g 5g 5g
Salt 3g 3g 3g
2.4. Cooking Noodles:
Noodle samples were cooked for 10-15 minutes in
salted boiling water at 85°C in a small stainless steel
thick-bottomed saucepan occasionally stirred with a
wooden kitchen spoon to prevent sticking, then
strained, rinsed, and washed with cooled running
water. Before testing noodles, it was strained from the
cooking water and placed in a plastic airtight
container for evaluation.
2.5. Chemical Composition Analysis:
The samples underwent chemical quality evaluation
using hot air oven for drying, and moisture was
measured. The incineration method was used to
calculate the total ash, The Roese-Gottlieb method
was used to determine fat, while the Kjeldahl method
was used to determine protein. Carbohydrate was
determined by FAO chapter-2, Methods of food
analysis, 2003, energy was determined by FAO
chapter-3: Calculation of the energy content of food
energy conversion factors, 2003
2.6. Organoleptic Quality analysis procedure:
The most common method for measuring food
preferences is a questionnaire of manufactured foods
or food categories, with a hedonic scale used to rate
the degree of liking. The hedonic scale is an
organoleptic quality rating scale on which the judge
expresses his level of liking. The overall tests were
carried out on a 9-point Hedonic scale. The
organoleptic Hedonic scale was used in the following
ratings: 1) Dislike extremely, 2) Dislike moderately,
3) Dislike moderately, 4) Dislike slightly, 5) Neither
like nor dislike, 6) Likely slightly, 7) Like
moderately, 8) Like extremely, 9) Like extremely.
This test was conducted by semi-trained panellists
who were teachers at Parul University's Department
of Food Technology in Vadodara, Gujarat.
2.7. Microbiology analysis procedure:
Microbiological tests such as the total coliform count
(TCC) and E. coli count were carried out in
accordance with Indian standard procedures for the
inspection of dairy products.
2.8. Mineral testing:
Mineral detection in food and feed can assist in
determining the appropriate daily portion and
supplement level. The amount of ash in a food is a
measure of the total amount of minerals present,
whereas mineral content is the amount of specific
inorganic components present in a food, such as Ca,
Na, K, and Cl. Nutritional labeling quality,
microbiological stability, nutrition, and processing all
rely on it.
Minerals like potassium (K) and calcium (Ca)
determine by ASE/ SOP/ 005/19 flame photometry
method.
3. Results and Discussion:
3.1. Organoleptic analysis of different
formulation of noodles:
Flavor
Taste is very important in evaluating food products.
The tongue is the human organ responsible for
assessing taste which has receptors that can detect
different flavors in food ingredients. The presence of
the tongue can detect the taste of food products
because the tongue has a good response to flavors in
food such as sour, sweet, salty, and bitter. Taste is
regarded as an important factor in deciding whether to
accept or reject a food or beverage product. A
concentration affects the taste value of wet noodles.
Making wet noodles with 5% Moringa oleifera leaf
powder, 70% sorghum flour, and 10% rice flour
yields the highest test value and is the most preferred
proportion. The taste value is known based on the
results of the analysis. The use of Moringa oleifera
leaf powder at 15% yields a very low yield, implying
International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470
@ IJTSRD | Unique Paper ID – IJTSRD53877 | Volume – 7 | Issue – 2 | March-April 2023 Page 38
that the panelists dislike the product. As more
Moringa oleifera leaf powder is added to the dough,
the taste becomes slightly bitter. Thus, increasing the
concentration of Moringa oleifera leaf powder affects
taste preference, i.e. panelists dislike the taste. The
increase in Moringa oleifera leaf powder
concentration imparts an aftertaste to the noodle
product.
Aroma:
A scoring scale can be used to assess the aroma of a
food or beverage product. The aroma of the noodles
changes due to the addition of Moringa oleifera leaf
powder. This aroma appears because Moringa
oleifera leaves contain compounds that evaporate
easily, so when added to the noodle mixture, it
evaporates and can be detected by a panelist. Moringa
oleifera leaves contain compounds that are highly
volatile (easily evaporated). Volatile compounds can
easily enter the olfactory system (olfactory).
Aromatic compounds are found in all plants or fruits
and are frequently used as flavoring agents in
manufactured foods and beverages. According to the
test, panelists tended to prefer the aroma of the
noodles when treated with Moringa oleifera leaf
powder at the lowest concentration of 5%, as opposed
to when noodles were produced as a control, which
had the greatest aroma preference rating. Test
panelists, however, did not enjoy the aroma of the
noodles after the concentration of Moringa oleifera
leaf powder was increased by 10% and 15%.
Color:
Color is a vision in food or beverage products that the
eye can detect quickly and directly (eyes). The colour
of food and drink creates an attractive impression.
This means that there is a connection or a difference
in the colour value of the resulting noodle product.
Moringa oleifera leaf powder had a significant impact
on each treatment. The test value decreased as the
concentration of Moringa oleifera leaf powder (%
used) increased. The treatment with the least amount
of Moringa oleifera leaf powder produced the highest
test value for the color. The more Moringa oleifera
leaf powder is used in the dough, the less appealing
the colour of the noodles. The resulting colour is
becoming more intense.
Texture:
The texture of the resulting noodles is valued
differently. This means that there is a connection or a
difference in the texture value of the resulting noodle
product. The higher the concentration of Moringa
oleifera leaf powder used in the dough, the more
texture the noodle product has. Panelists preferred the
addition of 5% Moringa oleifera leaf powder
concentration over 10% and 15% additions.
3.2. Chemical composition of various noodles preparation:
Chemical composition of formulated noodles samples is presented in table 3.2.1 Moisture, total ash, protein, fat,
carbohydrate, sugar and energy.
Constituents Units Results T0
Moisture % 7.62
Total ash % 5.48
Fat g/100g 1.86
Protein g/100g 6.80
Carbohydrate g/100g 78.24
Sugar g/100g 0.0
Energy Kcal/100g 356.9
Table 3.2.1 Chemical composition of various noodles preparation
International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470
@ IJTSRD | Unique Paper ID – IJTSRD53877 | Volume – 7 | Issue – 2 | March-April 2023 Page 39
Graph 3.2.1 Chemical composition of various noodles preparation
3.3. Microbiological quality assessments of noodles:
3.3.1. Coliform count:
Coliform bacteria are classified as rod-shaped, Gram-negative, nonspore-forming, motile, or nonmotile bacteria.
When cultured at 35–37°C, these bacteria may ferment lactose and produce acid and gas. They are frequently
used as a gauge for the cleanliness of food and water.
3.3.2. E.coli count:
E. coli bacteria typically live in the intestines of healthy humans and animals. Most E. coli strains are either
innocuous or very briefly cause diarrhea. However, other strains, including E. coli O157:H, can cause vomiting,
severe stomach pains, and bloody diarrhea.
E. coli can be spread by contaminated food or drink, particularly raw vegetables and undercooked ground beef.
E. coli O157:H7 infections in healthy persons often resolve within a week. A life-threatening kind of renal
failure is more likely to affect young kids and older adults.
Microbial parameters units Results (T0)
E.coli count Cfu per g absent
Coliform count Per 25 g Absent
Table 3.3 Microbiological quality assessments of noodles
3.4. Mineral composition of various noodles preparation:
The proper daily portion and the amount of supplements required can be determined by checking for minerals in
diet and feed. Ash content is a measurement of the total amount of minerals in a food, whereas mineral content is
the quantity of individual inorganic ingredients like Ca, Na, K, and Cl that are present in a food. Nutritional
labeling, quality, microbiological stability, nutrition, and processing all depend on it.
mineral units Results
Potassium (as K) Mg/100g 353.3
Calcium (as Ca) Mg/100g 232.4
Table 3.4 Mineral composition of various noodles preparation
4. Conclusions:
A higher concentration of Moringa oleifera leaf
powder has a significant impact on the sensory
properties of noodle. A higher concentration of
moringa leaf powder reduces sensory acceptability
while increasing antinutritional content, so it should
be considered in product development. Moringa
leaves, with a maximum usage limit of 5%, have the
potential to increase essential nutrients and minerals
in processed food.
5. References:
[1] Y. C. Huang and H. M. Lai, “Noodle quality
affected by different cereal starches,” Journal of
Food Engineering, vol. 97, no. 2, pp. 135–143,
2010
[2] M. Li, K. X. Zhu, X. N. Guo, K. Brijs, and H.
M. Zhou, “Natural additives in wheat-based
pasta and noodle products: opportunities for
enhanced nutritional and functional properties,”
International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470
@ IJTSRD | Unique Paper ID – IJTSRD53877 | Volume – 7 | Issue – 2 | March-April 2023 Page 40
Comprehensive Reviews in Food Science and
Food Safety, vol. 13, no. 4, pp. 347–357, 2014.
[3] C. M. Tudorica, V. Kuri, and C. S. Brennan,
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[4] J. M. M. Engels and E. Goettsch, Konso
Agriculture and Its Plant Genetic Resources,
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[5] Adi, A. C., Rachmah, Q. & Arimbi, A. N.
(2019) The acceptance and nutritional value of
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food insecure area. Preventive Nutrition and
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https://doi.org/10.3746/pnf.2019.24.4.387
[6] Alemayehu, D., Desse, G., Abegaz, K.,
Desalegn, B. B., & Getahun, D. (2016).
Proximate, Mineral Composition and Sensory
Acceptability of Home Made Noodles from
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https://doi.org/10.5923/j.food.20160603.02
[7] Olorunsogo, S.T., Adebayo, S E., Orhevba,
B.A., & Awoyinka, T.B. (2019).
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produced from blends of sweet potato, soybean
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[8] https://www.researchgate.net/publication/36629
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%20of%20higher,different%20effect%20on%2
0the%20sensory%20parameter%20of%20noodl
es
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al_chemical_and_sensory_quality_of_noodles_
fortification_with_anchovy_Stolephorus_sp_flo
u
[11] https://pdfs.semanticscholar.org/e1ab/7fb3000a
8303b18bfe368e22e39ee18fc759.pdf#:~:text=
Noodles%20are%20foods%20that%20are%20o
ften%20consumed%20by,or%20smaller%20co
ncentrations%20of%20Moringa%20oleifera%2
0leaf%20powder
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[13] Nikita Vilas Teke, Karuna Wasudeo Patil,
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322072285
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Int.Res.J.Nutr. 1(1):6-9

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Development of Sorghum Noodles Fortification with Moringa Moringa Oleifera Leaf Powder

  • 1. International Journal of Trend in Scientific Research and Development (IJTSRD) Volume 7 Issue 2, March-April 2023 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470 @ IJTSRD | Unique Paper ID – IJTSRD53877 | Volume – 7 | Issue – 2 | March-April 2023 Page 35 Development of Sorghum Noodles Fortification with Moringa (Moringa Oleifera) Leaf Powder Muskan Bhavesh Patel1 , Dhanya Joseph2 1 MSc Student, Department of Food Technology, 2 Assistant Professor, Department of Food Technology, 1,2 Parul Institute of Applied Science, Parul University, Waghodia, Vadodara, Gujarat, India ABSTRACT The study of noodles from sorghum flour, rice flour, and, Moringa oleifera leaf powder development was carried out at the Department of Food Technology, Parul Institute of Applied Sciences, Parul University, Vadodara Gujarat, India. The cereal grain sorghum has a number of agronomic benefits, contains substances that are good for your health, and is gluten-free. One of the most beneficial tropical trees is Moringa Oleifera, which offers vital nutrients and antioxidants to treat vitamin and mineral deficiencies, support a healthy cardiovascular system, help to promote normal blood sugar levels, neutralize free radicals, improve eyesight, mental clarity, and bone strength, and may be helpful in cases of malnutrition, general weakness, lactating mothers, menopause, depression, and osteoporosis. Moringa is a multi-beneficial plant with high nutritional value, making it an excellent protein supplement for malnutrition and animal feed. Although Moringa oleifera leaves are high in nutrients, the addition of saponins may increase the bitter taste and unpleasant smell. This study set out to create noodles using moringa leaf powder, sorghum flour, and rice flour and to assess their sensory acceptance, antinutritional content and proximate composition. The findings revealed in moisture (7.62), total ash (5.48), protein (6.80), fat (1.86), carbohydrate (78.24), energy (358.9), calcium (242.4) (Ca), and potassium (353.5) content, the total number of E. coli 0157, and coli form. In order to guarantee global food security and meet nutritional needs, the concentration of moringa plant powder must be limited. Moringa Oleifera leaves powder affected, macronutrient, and minerals content in wet noodle products. It is recommended that concentrations of 5%, 10%, and 15% should not exceed 5% because it affects the organoleptic of the product. How to cite this paper: Muskan Bhavesh Patel | Dhanya Joseph "Development of Sorghum Noodles Fortification with Moringa (Moringa Oleifera) Leaf Powder" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456- 6470, Volume-7 | Issue-2, April 2023, pp.35-40, URL: www.ijtsrd.com/papers/ijtsrd53877.pdf Copyright © 2023 by author (s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0) KEYWORDS: noodles, sorghum (jowar), Moringa oleifera (drumstick tree) leaf powder 1. INTRODUCTION Noodles are a widely consumed food item in many parts of the world. In many Asian nations, noodles have been considered as a staple food. In general, the benefit of instant noodles is that they are simple to prepare, delicious, and convenient to serve. It also has a lower cost and a longer shelf life (Ebtihal et al., 2019). Noodles can be made from a variety of flour such as rice, wheat, maize, finger millet (ragi), sorghum (jowar), and semolina using an extruder by adding water and flavorings such as egg and spice. Noodle consumption has been increasing, but the low fat and protein content limits its nutritional value. To improve the overall nutritional profile of noodles we, use sorghum flour, rice flour, and Moringa oleifera leaf powder. The sorghum crop is a vital food-security cereal crop in many places of Africa, Asia, and the semi-arid tropics around the world (FAO/WHO, 2007). In terms of production, Sorghum is the African continent's second most important cereal. This is because Africa's average sorghum production is around 20 million tonnes mt per year (FAO, 2019; Taylor, 2003; Collar, 2019). As a gluten-free grain, sorghum lacks viscoelastic properties. Protein (kafirin) derived from sorghum is more challenging to hydrate than wheat gluten protein. Sorghum increased starch content led IJTSRD53877
  • 2. International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470 @ IJTSRD | Unique Paper ID – IJTSRD53877 | Volume – 7 | Issue – 2 | March-April 2023 Page 36 to a high gelatinization temperature for starch and a low water-holding capacity Gluten protein is often required in fine-dried noodles to strengthen the noodle's resistance to breaking during the drying process (Arendt and Zannini, 2013). Sorghum's nutritional value is distinguished by its protein level, which ranges from 7 to 16% with an average of 11% (Taylor and Anyango, 2011). Cysteine was found in sorghum protein (Kafirin). When sorghum is cooked - wet, kafirin cross-linking via disulfide bonding occurs. As a result, sorghum grain protein is difficult to digest (Duodu et al., 2003). Moringa oleifera, also known as the drumstick tree, is a Moringaceae family member and is also known as the "Miracle Tree." (Mahato et al, 2022). Being a native of north-western India, it has received widespread recognition in ethnomedicine as well as the Ayurvedic and Unani medical systems. (Milla et al, 2021). According to Saifu (2015), the moringa tree leaf is used as a famine food to ensure food security. The moringa tree leaf, according to the NRC, contains essential nutrients such as protein, amino acids, carbohydrates, vitamins, minerals, and organic acid .(Oduro et al. and Madukwe (2013) According to reports, the moringa tree leaf also has antibacterial and anti-inflammatory properties. Product development using moringa leaf powder has not yet been properly customized and commercialized in developing countries such as Ethiopia, specifically Hawassa, despite the fact that moringa provides multiple benefits such as alleviating malnutrition and food insecurity, which are common problems in developing countries. Moringa has a wide range of nutritional advantages that are determined by factors like genetic make-up, environmental factors, and manufacturing techniques. For an animal to establish immunity(for the synthesis of antibodies and cells), proteins, minerals (zinc, copper, and iron), and vitamins (A and E) are also required. In order to combat infections, these nutrients also assist organs and tissues in communicating with one another. Moringa oleifera is recognized as a versatile plant because of its range of applications. Excellent sources of calcium, iron, and vitamins A, B, and C can be found in its leaves. The leaves of the Moringa plant are said to be particularly nutrient-dense since they have quantities of vitamin A, vitamin C, iron, calcium, and potassium that are comparable to those found in carrot, orange, spinach, and banana. Based on a comparison of its amino acid profiles with the FAO/WHO/UNO reference protein for children, it is likewise a good source of protein. High antioxidant activity levels found in moringa leaves have been linked to cancer chemoprevention, protein oxidation-reduction, and lipid peroxidation inhibition. The main purpose of this study is to assess the acceptability of noodles made with 70% sorghum flour, 10% rice flour, and 5% moringa (Moringa oleifera) leaf powder as well as other ingredients. The assessment was carried out among various samples of noodles (T0, T1, and T2) containing different amounts of Moringa oleifera leaf powder (5%, 10%, and 15%). To complete this noodles development experiment, chemical test, organoleptic test, mineral test, and microbiological test are. 2. Materials and Methods: 2.1. Materials: Sorghum flour, rice flour, and salt were purchased from the local market in Vadodara, Gujarat, India. Garlic and Ginger were purchased from the local market in Vadodara, Gujarat, India. After that garlic powder and ginger powder prepare in the Parul Institute of Applied Science Laboratory. however Moringa oleifera leaf powder was purchased from Amazon. Sorghum flour, rice flour, and moringa leaf powder were sieved into fine flour of uniform particles. The sorghum flour, rice flour, and moringa powder were later packed in a polyethylene bag and finally stored at room temperature. The sorghum flour, rice flour, and moringa leaf powder were then mixed according to the formulation. 2.2. Methods: Sorghum Noodle Preparation Noodles were prepared by using 70% sorghum flour, and 10% rice flour, with different proportions of Moringa oleifera leaf powder (5%, 10%, and 15%). Here, after samples are referred to as T0, T1, and T2 respectively. in the prepared sample T0 ,70% sorghum flour is used. Initially, for T1 and T2 samples rice flour and sorghum flour were mixed. In a large mixing bowl, combine the rice flour, sorghum flour, moringa leaf powder, ginger powder and garlic powder, and salt. Mix it well and slowly add the water, a little at a time, and mix the ingredients with your hands until you get dough that is smooth and elastic. If the dough is too dry, add a little more water. If it is too wet, add a little more flour. Wrap the dough in plastic wrap and let it rest for 15 minutes. Cut the dough into small pieces that will fit into the hand extruder. Place the dough into the hand extruder and turn the handle to push the dough through the extruder. The dough will come out in the shape of noodles. Finally, the extruded product (noodles) was cut into comparable sizes. Than it dried, packed, and stored at room temperature until cooked and served for sensory evaluation.
  • 3. International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470 @ IJTSRD | Unique Paper ID – IJTSRD53877 | Volume – 7 | Issue – 2 | March-April 2023 Page 37 Sorghum flour (70%) + rice flour (10%) Mixing all raw materials together (Ginger powder + garlic powder + Moringa oleifera leaf powder + salt) Dough making (flour + oil +water) Dough resting (15 minutes) Cut the dough into small pieces Extrusion (hand extruder) Drying (sun dry-15 hours) Packaging of noodles 2.3. Flow chart for the processing of noodles Formulation of sorghum noodles: Ingredients T0 T1 T2 Sorghum flour 70g 60g 60g Rice flour 10g 17g 12g Moringa leaf powder 5g 10g 15g Ginger powder 5g 5g 5g Garlic powder 5g 5g 5g Salt 3g 3g 3g 2.4. Cooking Noodles: Noodle samples were cooked for 10-15 minutes in salted boiling water at 85°C in a small stainless steel thick-bottomed saucepan occasionally stirred with a wooden kitchen spoon to prevent sticking, then strained, rinsed, and washed with cooled running water. Before testing noodles, it was strained from the cooking water and placed in a plastic airtight container for evaluation. 2.5. Chemical Composition Analysis: The samples underwent chemical quality evaluation using hot air oven for drying, and moisture was measured. The incineration method was used to calculate the total ash, The Roese-Gottlieb method was used to determine fat, while the Kjeldahl method was used to determine protein. Carbohydrate was determined by FAO chapter-2, Methods of food analysis, 2003, energy was determined by FAO chapter-3: Calculation of the energy content of food energy conversion factors, 2003 2.6. Organoleptic Quality analysis procedure: The most common method for measuring food preferences is a questionnaire of manufactured foods or food categories, with a hedonic scale used to rate the degree of liking. The hedonic scale is an organoleptic quality rating scale on which the judge expresses his level of liking. The overall tests were carried out on a 9-point Hedonic scale. The organoleptic Hedonic scale was used in the following ratings: 1) Dislike extremely, 2) Dislike moderately, 3) Dislike moderately, 4) Dislike slightly, 5) Neither like nor dislike, 6) Likely slightly, 7) Like moderately, 8) Like extremely, 9) Like extremely. This test was conducted by semi-trained panellists who were teachers at Parul University's Department of Food Technology in Vadodara, Gujarat. 2.7. Microbiology analysis procedure: Microbiological tests such as the total coliform count (TCC) and E. coli count were carried out in accordance with Indian standard procedures for the inspection of dairy products. 2.8. Mineral testing: Mineral detection in food and feed can assist in determining the appropriate daily portion and supplement level. The amount of ash in a food is a measure of the total amount of minerals present, whereas mineral content is the amount of specific inorganic components present in a food, such as Ca, Na, K, and Cl. Nutritional labeling quality, microbiological stability, nutrition, and processing all rely on it. Minerals like potassium (K) and calcium (Ca) determine by ASE/ SOP/ 005/19 flame photometry method. 3. Results and Discussion: 3.1. Organoleptic analysis of different formulation of noodles: Flavor Taste is very important in evaluating food products. The tongue is the human organ responsible for assessing taste which has receptors that can detect different flavors in food ingredients. The presence of the tongue can detect the taste of food products because the tongue has a good response to flavors in food such as sour, sweet, salty, and bitter. Taste is regarded as an important factor in deciding whether to accept or reject a food or beverage product. A concentration affects the taste value of wet noodles. Making wet noodles with 5% Moringa oleifera leaf powder, 70% sorghum flour, and 10% rice flour yields the highest test value and is the most preferred proportion. The taste value is known based on the results of the analysis. The use of Moringa oleifera leaf powder at 15% yields a very low yield, implying
  • 4. International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470 @ IJTSRD | Unique Paper ID – IJTSRD53877 | Volume – 7 | Issue – 2 | March-April 2023 Page 38 that the panelists dislike the product. As more Moringa oleifera leaf powder is added to the dough, the taste becomes slightly bitter. Thus, increasing the concentration of Moringa oleifera leaf powder affects taste preference, i.e. panelists dislike the taste. The increase in Moringa oleifera leaf powder concentration imparts an aftertaste to the noodle product. Aroma: A scoring scale can be used to assess the aroma of a food or beverage product. The aroma of the noodles changes due to the addition of Moringa oleifera leaf powder. This aroma appears because Moringa oleifera leaves contain compounds that evaporate easily, so when added to the noodle mixture, it evaporates and can be detected by a panelist. Moringa oleifera leaves contain compounds that are highly volatile (easily evaporated). Volatile compounds can easily enter the olfactory system (olfactory). Aromatic compounds are found in all plants or fruits and are frequently used as flavoring agents in manufactured foods and beverages. According to the test, panelists tended to prefer the aroma of the noodles when treated with Moringa oleifera leaf powder at the lowest concentration of 5%, as opposed to when noodles were produced as a control, which had the greatest aroma preference rating. Test panelists, however, did not enjoy the aroma of the noodles after the concentration of Moringa oleifera leaf powder was increased by 10% and 15%. Color: Color is a vision in food or beverage products that the eye can detect quickly and directly (eyes). The colour of food and drink creates an attractive impression. This means that there is a connection or a difference in the colour value of the resulting noodle product. Moringa oleifera leaf powder had a significant impact on each treatment. The test value decreased as the concentration of Moringa oleifera leaf powder (% used) increased. The treatment with the least amount of Moringa oleifera leaf powder produced the highest test value for the color. The more Moringa oleifera leaf powder is used in the dough, the less appealing the colour of the noodles. The resulting colour is becoming more intense. Texture: The texture of the resulting noodles is valued differently. This means that there is a connection or a difference in the texture value of the resulting noodle product. The higher the concentration of Moringa oleifera leaf powder used in the dough, the more texture the noodle product has. Panelists preferred the addition of 5% Moringa oleifera leaf powder concentration over 10% and 15% additions. 3.2. Chemical composition of various noodles preparation: Chemical composition of formulated noodles samples is presented in table 3.2.1 Moisture, total ash, protein, fat, carbohydrate, sugar and energy. Constituents Units Results T0 Moisture % 7.62 Total ash % 5.48 Fat g/100g 1.86 Protein g/100g 6.80 Carbohydrate g/100g 78.24 Sugar g/100g 0.0 Energy Kcal/100g 356.9 Table 3.2.1 Chemical composition of various noodles preparation
  • 5. International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470 @ IJTSRD | Unique Paper ID – IJTSRD53877 | Volume – 7 | Issue – 2 | March-April 2023 Page 39 Graph 3.2.1 Chemical composition of various noodles preparation 3.3. Microbiological quality assessments of noodles: 3.3.1. Coliform count: Coliform bacteria are classified as rod-shaped, Gram-negative, nonspore-forming, motile, or nonmotile bacteria. When cultured at 35–37°C, these bacteria may ferment lactose and produce acid and gas. They are frequently used as a gauge for the cleanliness of food and water. 3.3.2. E.coli count: E. coli bacteria typically live in the intestines of healthy humans and animals. Most E. coli strains are either innocuous or very briefly cause diarrhea. However, other strains, including E. coli O157:H, can cause vomiting, severe stomach pains, and bloody diarrhea. E. coli can be spread by contaminated food or drink, particularly raw vegetables and undercooked ground beef. E. coli O157:H7 infections in healthy persons often resolve within a week. A life-threatening kind of renal failure is more likely to affect young kids and older adults. Microbial parameters units Results (T0) E.coli count Cfu per g absent Coliform count Per 25 g Absent Table 3.3 Microbiological quality assessments of noodles 3.4. Mineral composition of various noodles preparation: The proper daily portion and the amount of supplements required can be determined by checking for minerals in diet and feed. Ash content is a measurement of the total amount of minerals in a food, whereas mineral content is the quantity of individual inorganic ingredients like Ca, Na, K, and Cl that are present in a food. Nutritional labeling, quality, microbiological stability, nutrition, and processing all depend on it. mineral units Results Potassium (as K) Mg/100g 353.3 Calcium (as Ca) Mg/100g 232.4 Table 3.4 Mineral composition of various noodles preparation 4. Conclusions: A higher concentration of Moringa oleifera leaf powder has a significant impact on the sensory properties of noodle. A higher concentration of moringa leaf powder reduces sensory acceptability while increasing antinutritional content, so it should be considered in product development. Moringa leaves, with a maximum usage limit of 5%, have the potential to increase essential nutrients and minerals in processed food. 5. References: [1] Y. C. Huang and H. M. Lai, “Noodle quality affected by different cereal starches,” Journal of Food Engineering, vol. 97, no. 2, pp. 135–143, 2010 [2] M. Li, K. X. Zhu, X. N. Guo, K. Brijs, and H. M. Zhou, “Natural additives in wheat-based pasta and noodle products: opportunities for enhanced nutritional and functional properties,”
  • 6. International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470 @ IJTSRD | Unique Paper ID – IJTSRD53877 | Volume – 7 | Issue – 2 | March-April 2023 Page 40 Comprehensive Reviews in Food Science and Food Safety, vol. 13, no. 4, pp. 347–357, 2014. [3] C. M. Tudorica, V. Kuri, and C. S. Brennan, “Nutritional and physicochemical characteristics of dietary fiber enriched pasta,” Journal of Agricultural and Food Chemistry, vol. 50, no. 2, pp. 347–356, 2002. [4] J. M. M. Engels and E. Goettsch, Konso Agriculture and Its Plant Genetic Resources, Plant Genetic Resources of Ethiopia Cambridge University Press, Cambridge, 1991. file:///D:/downlods/Proximate_Composition_A ntinutritional_Co%20(9).pdf [5] Adi, A. C., Rachmah, Q. & Arimbi, A. N. (2019) The acceptance and nutritional value of crispy noodles supplemented with moringa oleifera as a functional snack for children in a food insecure area. Preventive Nutrition and Food Science, 24(4), 387–392. https://doi.org/10.3746/pnf.2019.24.4.387 [6] Alemayehu, D., Desse, G., Abegaz, K., Desalegn, B. B., & Getahun, D. (2016). Proximate, Mineral Composition and Sensory Acceptability of Home Made Noodles from Stinging Nettle (Urtica simensis) Leaves and Wheat Flour Blends International Journal of Food Science and Nutrition Engineering, 6(3), 55–61. https://doi.org/10.5923/j.food.20160603.02 [7] Olorunsogo, S.T., Adebayo, S E., Orhevba, B.A., & Awoyinka, T.B. (2019). Physicochemical properties of instant noodles produced from blends of sweet potato, soybean and corn flour. Food Research, 3(5), 391–399. https://doi.org/10.26656/fr.2017.3(5).304 [8] https://www.researchgate.net/publication/36629 8297_Fortification_of_Moringa_oleifera_Flour _on_Quality_of_Wet_Noodle#:~:text=The%20 results%20showed%20that%20the%20addition %20of%20higher,different%20effect%20on%2 0the%20sensory%20parameter%20of%20noodl es [9] Fu, B.X. Asian noodles: history, classification, raw materials, and processing. Food Research International 41: 888-902 (2008) http://ppaspk.org/index.php/PPAS- B/article/view/339/280 [10] researchgate.net/publication/359928197_Physic al_chemical_and_sensory_quality_of_noodles_ fortification_with_anchovy_Stolephorus_sp_flo u [11] https://pdfs.semanticscholar.org/e1ab/7fb3000a 8303b18bfe368e22e39ee18fc759.pdf#:~:text= Noodles%20are%20foods%20that%20are%20o ften%20consumed%20by,or%20smaller%20co ncentrations%20of%20Moringa%20oleifera%2 0leaf%20powder [12] Fei Xu, Nhlanhla Mtelisi Dube, Han, Renyong Zhao, Yuanhui Wang, Jie Chen, (2021).The effect of Zimbabwean tannin-free sorghum flour substitution on fine dried noodles quality characteristics Journal of Cereal Science. [13] Nikita Vilas Teke, Karuna Wasudeo Patil, Hemangi Jayram Gavit (2023) Formulation of healthy cookies incorporated with orange peel powder and Moringa oleifera leaf powder. sciencedirect.com/science/article/pii/S2214785 322072285 [14] Oyeyinka, A. T., & Oyeyinka, S. A. (2018). Moringa oleifera as a food fortificant: Recent trends and prospects. Journal of the Saudi Society of Agricultural Sciences, 17(2), 127– 136. https://doi.org/10.1016/j.jssas.2016.02.002 [15] Prayitno, S. A., & Rahim, A. R. (2021). The Proportion of Moringa and Cassava Leaves on the Chemical and Sensory Properties of Chicken Nuggets Journal of Physics: Conference Series, 1764(1). https://doi.org/10.1088/1742- 6596/1764/1/012032. [16] Sutrisno Adi Prayitno, Nur Agustin Mardiana, Nurthalita Alifia Rochma (2021) Sensory Evaluation of Wet Noodle Products Added with Moringa Oleifera Flour with Different Concentrations. journal.umg.ac.id/index.php/kontribusia/article/ view/2738/1727 [17] Chandra S., Pradeep D., Arti., Anjana and LP Shinde. (2019). Significance of Moringa Noodles for Increasing Breast Milk. Int.Res.J.Nutr. 1(1):6-9