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Phylogenetics:
Classification of
Life
ppt. by Robin D. Seamon
1
HOOK VIDEO: COSMOS-
“Some Things Molecules do”
2 TYPES OF CELLS
Prokaryotes
• Oldest life forms
• Cell wall
• Cell membrane
• cytoplasm
• single chromosome: circular
strand of DNA
• Reproduction: binary fission
Eukaryotes
• (Plants, fungi, some protists have
cell wall)
• Cell membrane
• Cytoplasm
• DNA inside a membrane-bound
nucleus
• Organelles to perform metabolic
and reproductive processes
• Reproduction: asexual or sexual
reproduction
2
VIDEO: How we think complex organisms
evolved endosymbiosis (5:40)
REVIEW
3 Domains:
Prokaryote: no nucleus or organelles
Eukaryotes: nucleus & membrane-bound organelles
VIDEO: How we think complex cells evolved (5:30)
3
NEW There are 3
Domains
K
C
O
F
S
G
P
RANKING
SYSTEM
4
5
CC video: comparative
anatomy (10 min)
Kingdom
Archae-
bacteria
H I S T O R Y
Aristotle: (Greek philosopher 384-322 CE)
classified animals based on method of
reproduction
6
Taxonomy: group or categorize organisms
• Before 1700, there were multiple long names for new
species with no rules in naming process
Carl Linnaeus (Swedish) 1735
grouped organisms by shared physical characteristics
• wrote Systems Naturae
3 Kingdoms: 1. Mineral
2. Vegetable
3. Animal
7
8
Binomial nomenclature
• Linneaus’ 2-name system
• Scientific name = species name
• Genus species
• First part – genus - capitalized
• Second part - species identifier
• Both italicized or underlined
Today naming is
regulated by
Nomenclature
Codes allowing
names to be
divided into ranks:
Binomial
nomenclature
(genus & species)
1740’s
R A N K I N G
S Y S T E M
9CC Video: #19 Taxonomy(12 min)
• 1859 Darwin insisted classification should
reflect genetic relatedness
• 1900 Emil Willi Henning: grouped organisms by
inferred evolutionary relatedness
• Homologous structures; inherited
from common ancestors, so were
related
Phylogenetic taxonomy
10
• Cladistic system: 1960’s scientists started using
DNA sequences to determine common ancestry
IGNORING RANKS
• This new field is making changes & revisions in
classification everyday.
• International Code of Phylogenetic
Nomenclature (PhyloCode) is currently under
development
Molecular Phylogenetics
C L A D E S Y S T E M
11
12
Cladistics
Uses derived character
• A feature that evolved only within a group
• Used to establish evolutionary relationships
13
MODERN SYSTEM
Three-Domain System
• Compare ribosomal RNA
• All cells have ribosomes
Bacteria, Archaea, Eukarya
• With Six-Kingdoms as subgroups
14
• 1990’s: New Grouping based on new discoveries
in bacteria metabolism
1. Archaea
2. Bacteria
3. Eukaryota
D O M A I N S
Separated
these 2
Includes Protists
15
CC Video: #35 Archaea, Bacteria, Protists (12 min)
Prokayotes have evolved
longer & are more flexible in
their biochemistry, therefore
they can live in more
extreme environments than
eukaryotes
--Many, but not all are
autotrophic (make their own
sugars)
• phototropic organisms
• chemotrophic organisms
Eukaryotes perform
metabolism in the organelles
of the cells
-- All but plants are
heterotrophic
• Chloroplasts:
photosynthesis
• Mitochondria:
cellular respiration
PROKARYOTES EUKARYOTES
16
REVIEW
17
• REPRODUCTION: binary fission
• TRANSPORT: through the membrane
Archaea & Bacteria
18
Archaea- ancient group of prokaryotic
organisms that are still present today --
Extremphiles- types of archaea that live in very
extreme places
Some examples:
• Halophile: live in extreme salty
conditions
• Thermophile: live in extremely hot
conditions
• Methanogen: metabolize such that
they use acidic conditions (sulfur)
and release methane
PROKARYOTE:
No nucleus or
organelles
DNA in
cytoplasm
Binary fission
Kingdom Archaebacteria
19
Hiller Lake, Australia
Halophilic archaea & bacteria live in this salty
lake BACK
Under-ocean
hot vent
20
Yellowstone hot vent, Wyoming
thermophilic archaea & bacteria live in this
hot vent BACK
21
Methanogenic archaea
BACK
22
PROKARYOTE:
No nucleus or
organelles
DNA in
cytoplasm
Binary fission
Bacteria- ancient group of prokaryotes
that are still present today
Kingdom Eubacteria
23
Bacteria in our world:
• Diseases
• Nitrogen-fixing
• Digestion
• Make vitamins
• Food
production
Diseases Caused by Bacteria
Disease Transmission Symptoms Treatment
Strep throat
(Streptococcus)
Inhale or
ingest through
mouth
Fever, sore throat,
swollen neck glands
Antibiotic
Tuberculosis Inhale Fatigue, fever, night
sweats, cough, weight
loss, chest pain
Antibiotic
Tetanus Puncture
wound
Stiff jaw, muscle
spasms, paralysis
Open and clean wound,
antibiotic; give antitoxin
Lyme disease Bite of
infected tick
Rash at site of bite,
chills, body aches,
joint swelling
Antibiotic
Dental
cavities (caries)
Bacteria
in mouth
Destruction of tooth
enamel, toothache
Remove and fill the
destroyed area of tooth
Diptheria Inhale or
close contact
Sore throat, fever,
heart or breathing
failure
Vaccination to
prevent, antibiotics
24
Eukaryota- group of organisms whose cells
contain a nucleus and membrane-bound
organelles
ALL THE REST
4 Kingdoms
Phylogenetic tree
25
PROKARYOTES EUKARYOTES
Archaebacteria
(Archaea)
Eubacteria
(Bacteria)
Kingdom Protista-
(microscopic
organisms)
Kingdom Plantae-
Kingdom Fungi
Kingdom Animalia
26
DOMAIN
BACTERIA
DOMAIN
ARCHAEA DOMAIN
BACTERIA
REVIEW
6 Kingdoms
PLANT KINGDOM
specific organelles
(autotrophs)
• Large central
vacuole
• No lysosome,
but the recycling
can happen in
the vacuoles
• Plastids
• Chloroplasts
• Chromoplasts
• Leukoplasts
ANIMAL KINGDOM
specific organelles
(heterotrophs)
• Smaller vacuoles
• lysosomes
FUNGI KINGDOM
closer related to
animal than plant
(heterotrophic)
• No lysosome, but
the recycling can
happen in the
vacuoles
PROTIST- microscopic organisms of a huge variety; some more related
to plant metabolism (autotrophic), others more related to
heterotrophs (fungi/animal)
MICRO
MACRO
COMPARISONS of EUKARYOTES
27
REVIEW (have nucleus &
organelles)
Protists- eukaryotic microscopic organisms;
Kingdom Protista
• Most are single-celled, though some are
multi-celled; some are colonial
• Some are more related to plant metabolism
(autotrophic), others more related to
heterotrophs (fungi/animal)
• Asexual reproduction: mitosis
• Transport: through membrane
KINGDOM PROTISTA
H I S T O R Y O F C L A S S I F I C A T I O N
1735
Linnaeus
1866
Haeckel
1925
Chatton
1938
Copeland
1969
Whittaker
1990
Woesse
1998
Cavalier-
Smith
2 Kingdoms 3 Kingdoms 2 Empires 4 Kingdoms 5 Kingdoms 3 Domains 6 Kingdoms
Mineral Prokaryote Monera Monera Bacteria Bacteria
Protist Protist Protist Archaea Protist
Eukaryote Eukaryote Chromista
Vegetable Plant Plant Plant Plant
Fungi Fungi
Animal Animal Animal Animal Animal
To apply
Darwin to
Microscopic
organisms
To show
having
nucleus or
not
To show
evolutionary
history of
life
29
1969 Whittaker: 5 Kingdoms
Protists
Monera
Plants
Animals
Fungi
Prokaryotes:
Eubacteria &
Archeobacteria
Eukaryotes
Absorb & photosynthesize
Asexual Reproduction
Absorb & photosynthesize
Asexual & Sexual Reproduction
Photosynthesize
Asexual & Sexual Reproduction
Non-mobile
Ingest
Asexual & Sexual Reproduction
mobile
Absorb
Spores: asexual Reproduction
Non-mobile
30
1990 Woese: 3 Domains
Eukaryotes
Plants
Animals
Fungi
Protists
Photosynthesize
Asexual & Sexual
Reproduction
Non-mobile
Absorb
Spores: asexual
Reproduction
Non-mobile
Archaea CBacteria
Monera Monera
Absorb & photosynethsize
Aerobic respiration
Asexual & Sexual Reproduction
Various metabolism:
photosynthesis, fermentation,
Methanogenesis respiration
Asexual Reproduction
Various metabolism:
photosynthesis, fermentation,
Anaerobic & aerobic respiration
Asexual Reproduction
Ingest
Asexual & Sexual
Reproduction
mobile
NO
NUCLEUS
NO
NUCLEUS
NUCLEUS
DOMAIN DOMAIN DOMAIN
31
NOTES LAB
1990 Woese: 3 Domains
Eukaryotes
Plants
Animals
Fungi
Protists
Photosynthesize
Asexual & Sexual
Reproduction
Non-mobile
Absorb
Spores: Asexual
Reproduction
Non-mobile
Archaea CBacteria
Monera Monera
Absorb & photosynethsize
Aerobic respiration
Asexual & Sexual Reproduction
Various metabolism:
photosynthesis, fermentation,
Methanogenesis respiration
Asexual Reproduction
Various metabolism:
photosynthesis, fermentation,
Anaerobic & aerobic respiration
Asexual Reproduction
Ingest
Asexual & Sexual
Reproduction
mobile
NO
NUCLEUS
NO
NUCLEUS
NUCLEUS
DOMAIN DOMAIN DOMAIN
32
Protist
Fungi
Tree of life Web Project
Tolweb.org
33
34

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Phylogenetics: Classification Notes

  • 1. Phylogenetics: Classification of Life ppt. by Robin D. Seamon 1 HOOK VIDEO: COSMOS- “Some Things Molecules do”
  • 2. 2 TYPES OF CELLS Prokaryotes • Oldest life forms • Cell wall • Cell membrane • cytoplasm • single chromosome: circular strand of DNA • Reproduction: binary fission Eukaryotes • (Plants, fungi, some protists have cell wall) • Cell membrane • Cytoplasm • DNA inside a membrane-bound nucleus • Organelles to perform metabolic and reproductive processes • Reproduction: asexual or sexual reproduction 2 VIDEO: How we think complex organisms evolved endosymbiosis (5:40) REVIEW
  • 3. 3 Domains: Prokaryote: no nucleus or organelles Eukaryotes: nucleus & membrane-bound organelles VIDEO: How we think complex cells evolved (5:30) 3
  • 4. NEW There are 3 Domains K C O F S G P RANKING SYSTEM 4
  • 5. 5 CC video: comparative anatomy (10 min) Kingdom Archae- bacteria
  • 6. H I S T O R Y Aristotle: (Greek philosopher 384-322 CE) classified animals based on method of reproduction 6
  • 7. Taxonomy: group or categorize organisms • Before 1700, there were multiple long names for new species with no rules in naming process Carl Linnaeus (Swedish) 1735 grouped organisms by shared physical characteristics • wrote Systems Naturae 3 Kingdoms: 1. Mineral 2. Vegetable 3. Animal 7
  • 8. 8 Binomial nomenclature • Linneaus’ 2-name system • Scientific name = species name • Genus species • First part – genus - capitalized • Second part - species identifier • Both italicized or underlined
  • 9. Today naming is regulated by Nomenclature Codes allowing names to be divided into ranks: Binomial nomenclature (genus & species) 1740’s R A N K I N G S Y S T E M 9CC Video: #19 Taxonomy(12 min)
  • 10. • 1859 Darwin insisted classification should reflect genetic relatedness • 1900 Emil Willi Henning: grouped organisms by inferred evolutionary relatedness • Homologous structures; inherited from common ancestors, so were related Phylogenetic taxonomy 10
  • 11. • Cladistic system: 1960’s scientists started using DNA sequences to determine common ancestry IGNORING RANKS • This new field is making changes & revisions in classification everyday. • International Code of Phylogenetic Nomenclature (PhyloCode) is currently under development Molecular Phylogenetics C L A D E S Y S T E M 11
  • 12. 12 Cladistics Uses derived character • A feature that evolved only within a group • Used to establish evolutionary relationships
  • 13. 13 MODERN SYSTEM Three-Domain System • Compare ribosomal RNA • All cells have ribosomes Bacteria, Archaea, Eukarya • With Six-Kingdoms as subgroups
  • 14. 14
  • 15. • 1990’s: New Grouping based on new discoveries in bacteria metabolism 1. Archaea 2. Bacteria 3. Eukaryota D O M A I N S Separated these 2 Includes Protists 15 CC Video: #35 Archaea, Bacteria, Protists (12 min)
  • 16. Prokayotes have evolved longer & are more flexible in their biochemistry, therefore they can live in more extreme environments than eukaryotes --Many, but not all are autotrophic (make their own sugars) • phototropic organisms • chemotrophic organisms Eukaryotes perform metabolism in the organelles of the cells -- All but plants are heterotrophic • Chloroplasts: photosynthesis • Mitochondria: cellular respiration PROKARYOTES EUKARYOTES 16 REVIEW
  • 17. 17 • REPRODUCTION: binary fission • TRANSPORT: through the membrane Archaea & Bacteria
  • 18. 18 Archaea- ancient group of prokaryotic organisms that are still present today -- Extremphiles- types of archaea that live in very extreme places Some examples: • Halophile: live in extreme salty conditions • Thermophile: live in extremely hot conditions • Methanogen: metabolize such that they use acidic conditions (sulfur) and release methane PROKARYOTE: No nucleus or organelles DNA in cytoplasm Binary fission Kingdom Archaebacteria
  • 19. 19 Hiller Lake, Australia Halophilic archaea & bacteria live in this salty lake BACK
  • 20. Under-ocean hot vent 20 Yellowstone hot vent, Wyoming thermophilic archaea & bacteria live in this hot vent BACK
  • 22. 22 PROKARYOTE: No nucleus or organelles DNA in cytoplasm Binary fission Bacteria- ancient group of prokaryotes that are still present today Kingdom Eubacteria
  • 23. 23 Bacteria in our world: • Diseases • Nitrogen-fixing • Digestion • Make vitamins • Food production Diseases Caused by Bacteria Disease Transmission Symptoms Treatment Strep throat (Streptococcus) Inhale or ingest through mouth Fever, sore throat, swollen neck glands Antibiotic Tuberculosis Inhale Fatigue, fever, night sweats, cough, weight loss, chest pain Antibiotic Tetanus Puncture wound Stiff jaw, muscle spasms, paralysis Open and clean wound, antibiotic; give antitoxin Lyme disease Bite of infected tick Rash at site of bite, chills, body aches, joint swelling Antibiotic Dental cavities (caries) Bacteria in mouth Destruction of tooth enamel, toothache Remove and fill the destroyed area of tooth Diptheria Inhale or close contact Sore throat, fever, heart or breathing failure Vaccination to prevent, antibiotics
  • 24. 24 Eukaryota- group of organisms whose cells contain a nucleus and membrane-bound organelles ALL THE REST 4 Kingdoms
  • 26. PROKARYOTES EUKARYOTES Archaebacteria (Archaea) Eubacteria (Bacteria) Kingdom Protista- (microscopic organisms) Kingdom Plantae- Kingdom Fungi Kingdom Animalia 26 DOMAIN BACTERIA DOMAIN ARCHAEA DOMAIN BACTERIA REVIEW 6 Kingdoms
  • 27. PLANT KINGDOM specific organelles (autotrophs) • Large central vacuole • No lysosome, but the recycling can happen in the vacuoles • Plastids • Chloroplasts • Chromoplasts • Leukoplasts ANIMAL KINGDOM specific organelles (heterotrophs) • Smaller vacuoles • lysosomes FUNGI KINGDOM closer related to animal than plant (heterotrophic) • No lysosome, but the recycling can happen in the vacuoles PROTIST- microscopic organisms of a huge variety; some more related to plant metabolism (autotrophic), others more related to heterotrophs (fungi/animal) MICRO MACRO COMPARISONS of EUKARYOTES 27 REVIEW (have nucleus & organelles)
  • 28. Protists- eukaryotic microscopic organisms; Kingdom Protista • Most are single-celled, though some are multi-celled; some are colonial • Some are more related to plant metabolism (autotrophic), others more related to heterotrophs (fungi/animal) • Asexual reproduction: mitosis • Transport: through membrane KINGDOM PROTISTA
  • 29. H I S T O R Y O F C L A S S I F I C A T I O N 1735 Linnaeus 1866 Haeckel 1925 Chatton 1938 Copeland 1969 Whittaker 1990 Woesse 1998 Cavalier- Smith 2 Kingdoms 3 Kingdoms 2 Empires 4 Kingdoms 5 Kingdoms 3 Domains 6 Kingdoms Mineral Prokaryote Monera Monera Bacteria Bacteria Protist Protist Protist Archaea Protist Eukaryote Eukaryote Chromista Vegetable Plant Plant Plant Plant Fungi Fungi Animal Animal Animal Animal Animal To apply Darwin to Microscopic organisms To show having nucleus or not To show evolutionary history of life 29
  • 30. 1969 Whittaker: 5 Kingdoms Protists Monera Plants Animals Fungi Prokaryotes: Eubacteria & Archeobacteria Eukaryotes Absorb & photosynthesize Asexual Reproduction Absorb & photosynthesize Asexual & Sexual Reproduction Photosynthesize Asexual & Sexual Reproduction Non-mobile Ingest Asexual & Sexual Reproduction mobile Absorb Spores: asexual Reproduction Non-mobile 30
  • 31. 1990 Woese: 3 Domains Eukaryotes Plants Animals Fungi Protists Photosynthesize Asexual & Sexual Reproduction Non-mobile Absorb Spores: asexual Reproduction Non-mobile Archaea CBacteria Monera Monera Absorb & photosynethsize Aerobic respiration Asexual & Sexual Reproduction Various metabolism: photosynthesis, fermentation, Methanogenesis respiration Asexual Reproduction Various metabolism: photosynthesis, fermentation, Anaerobic & aerobic respiration Asexual Reproduction Ingest Asexual & Sexual Reproduction mobile NO NUCLEUS NO NUCLEUS NUCLEUS DOMAIN DOMAIN DOMAIN 31 NOTES LAB
  • 32. 1990 Woese: 3 Domains Eukaryotes Plants Animals Fungi Protists Photosynthesize Asexual & Sexual Reproduction Non-mobile Absorb Spores: Asexual Reproduction Non-mobile Archaea CBacteria Monera Monera Absorb & photosynethsize Aerobic respiration Asexual & Sexual Reproduction Various metabolism: photosynthesis, fermentation, Methanogenesis respiration Asexual Reproduction Various metabolism: photosynthesis, fermentation, Anaerobic & aerobic respiration Asexual Reproduction Ingest Asexual & Sexual Reproduction mobile NO NUCLEUS NO NUCLEUS NUCLEUS DOMAIN DOMAIN DOMAIN 32
  • 33. Protist Fungi Tree of life Web Project Tolweb.org 33
  • 34. 34