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Biological help for the human race
Biological help for the human race
BiOWay™ Super Microbe Functionality
Microbe Description Function
Pediococcus
acidilactici
Produces
Lactic acid &
Pediocins to:
inhibit growth of gram negative bacteria;
Vibrio cholerae, Salmonella sp., Pseudomonas sp., E.coli, Clamphylobacter etc.
inhibit growth of gram positive bacteria:
Clostridium botulinum,Clostridium perfringens,Staphylococcus aureus, Listeria monocytogens
Pediococcus
pentosaceus
Produces
Lactic acid &
Pediocins to:
inhibit growth of gram negative bacteria:
Vibrio cholerae, Salmonella sp., Pseudomonas sp., E.coli, Clamphylobacter etc.
and inhibit growth of gram positive bacteria as:
Clostridium botulinum, Clostridium perfringens, Staphylococcus aureus, Listeria monocytogens
Bacillus
amyloliquefaciens
Produces
Enzymes to:
break organic matter into smaller molecules:
Amylase, Lipase, Protease, Peptidase, Sucrase
Produces:
Iturins
(Antifungal)
to inhibit growth of fungi including:
Fusarium, Collectotricum, Rhizoctonia, Aspergillus, Phytopthera
Pichia farinosa Yeast rarely
found in
nature
creates Toxins Killer to inhibit the growth of yeasts and neutralizes a wide range of toxins:
Aflatoxins, Mycotoxins, Endotoxins, Exotoxins etc
Dekkera anomala Spoilage Beer
Yeast:
ferments carbohydrate, starch and sugar into Acetic acid without alcohol and produces
enzymes:
Cellulase, Hemicellulase, Xylanase, Cellubioase, Amylase, Pectinase, Lignase, Arabinase etc
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Man use many kinds of chemical in daily life
Putting our health and live into Danger
Environment becomes toxic
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“BIOWAY™ Enzymes and Microbes”
Effective Bio-products from Nature
Researched, Developed and Manufactured
In Thailand
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MANGROVES & BIOWAY MICROBES
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What is BIOWAY™?
BIOWAY™ is a revolutionary combination of human and
environmentally friendly active micro-organisms creating
a powerful cocktail of beneficial enzymes, super
catalyzing enzymes and co-factors with remarkable ability
to fully digest a wide range of compounds and sanitize the
environment.
It is a natural 100% organic, bio-degradable culture
without harsh chemicals or residues.
BIOWAY™ rapidly digests fats, protein, carbohydrate,
cellulose, and a wide range of pollutants leaving the
environment in natural balance.
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The power of BIOWAY™ is a newly discovered naturally occurring enzyme
known as ‘Super Catalase’.
This cultured protein creates a Kinetic Rate Increase for all other enzymes
of 1,000’s to 100,000’s of thousands of times their normal rate of catalysis.
For example:
This ability to enhance natural biochemical reaction speed makes BIOWAY
effective in many applications where previously no solution existed.
Combined with the broad spectrum enzyme formulation, Pediocins, Iturins
and toxins killer, our Kinetic Rate Enhancement makes BIOWAY products
much more effective than any other technology in a wide rage of
applications.
Enzyme Type Natural Kinetic Rate BIOWAY Rate
Enhancement
Lipase 5,000 / sec 10M / sec
Protease 25-30K / sec 1 Billion+ / sec
The World’s Most Powerful
Enzyme Technology
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Rapidly Degrade & Fully Digest
Non-Living Organic Matter
Anti-Microbial Anti-Fungal
Neutralises Toxins
Core Capabilities
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Applications
Environmental Odor Control
Waste Water Treatment
Waste Management
Soil Remediation
Ground Water Remediation
Biogas Production
Agricultural Animal Health / Feed Supplement
Cropping
Cleaning Cleaning / Grease Removal
Air-conditioning Cooling Towers
Cosmetic Soap, Shampoo, Deodorant, Facial Exfoliator, Body Firming Lotion
Consumer Products Compost Aid, Bin Wash, Septic Aid, Waste Digester, Pond & Tank
Treatment, Drain Cleaner
Human Health Pharmacological, Health Supplements
BIOWAY™ is currently used in a broad range of applications:
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BIOWAY™’ is superior performance across its many applications can be summarised as follows:
1. Very High Speed
‘Super Catalase’ the proprietary enzyme in BIOWAY™ makes products formulated with our
technology thousands of times faster than what was previously possible.
2. Broad Functionality
BIOWAY™’s 4 core capabilities of rapidly breaking down organic waste,
anti-microbial, anti-fungal and toxins neutralisation, with the speed enhancement makes
BIOWAY™ a truly unique technology.
3. Broad Spectrum
BIOWAY™ is formulated to created a very diverse group of catabolic hydrolysing enzymes. This
provides an ability to rapidly and fully digest all non-living matter that is organic in origin.
4. Broad Operative Range
BIOWAY™ due to its kinetic rate enhancement technology is able to operate in a much broader
range of environmental conditions than previously available technologies.
What Makes BIOWAY™ so unique?
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Consists of group of Beneficial
Microorganisms
Highly concentrate, Effective Acting
Mainly 5 microorganisms
3 Bacterias
2 Yeasts
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BIOWAY™ Microbe Functionality
Microbe Description Function
Pediococcus
acidilactici
Produces
Lactic acid &
Pediocins to:
inhibit growth of gram negative bacteria;
Vibrio cholerae, Salmonella sp., Pseudomonas sp., E.coli, Clamphylobacter etc.
inhibit growth of gram positive bacteria:
Clostridium botulinum, Clostridium perfringens, Staphylococcus aureus, Listeria monocytogens
Pediococcus
pentosaceus
Produces
Lactic acid &
Pediocins to:
inhibit growth of gram negative bacteria:
Vibrio cholerae, Salmonella sp., Pseudomonas sp., E.coli, Clamphylobacter etc.
and inhibit growth of gram positive bacteria as:
Clostridium botulinum, Clostridium perfringens, Staphylococcus aureus, Listeria monocytogens
Bacillus
amyloliquefaciens
Produces
Enzymes to:
break organic matter into smaller molecules:
Amylase, Lipase, Protease, Peptidase, Sucrase
Produces:
Iturins
(Antifungal)
to inhibit growth of fungi including:
Fusarium, Collectotricum, Rhizoctonia, Alternaria, Aspergillus, Phytopthera
Pichia farinosa Yeast rarely
found in
nature
creates Toxins Killer to inhibit the growth of yeasts and neutralizes a wide range of
toxins:
Aflatoxins, Mycotoxins, Endotoxins, etc
Dekkera anomala Spoilage
Beer Yeast:
ferments carbohydrate, starch and sugar into Acetic acid without alcohol and produces
enzymes:
Cellulase, Hemicellulase, Xylanase, Cellubioase, Amylase, Pectinase, Lignase, Arabinase etc
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Digest Organic
matter
Lactic acid
Bacteriocin
(Pediocins)
Gram-negative
- Salmonella
- E. coli
- Pseudomonas
- Vibrio cholerae
Gram-Positive
- Clostridium botulinum
- Clostridium perfiengens
- Staphylococcus aureus
- Listeria monocytogens
1. Pediococcus acidilactici Lactic Acid Bacteria [5.0x107 cfu/g]
 produce Lactic acid and Pediocins
 inhibit growth of gram negative bacteria as: Vibrio cholerae,
E. coli, Salmonella sp. etc.
 and inhibit growth of gram positive bacteria as: Clostridium botulinum,
Clostridium perfiengens, Staphylococcus aureus etc.
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Digest Organic
Matter
Lactic acid
Bacteriocin
(Pediocins)
Gram-negative
- Salmonella
- E. coli
- Pseudomonas
- Vibrio cholerae
Gram-Positive
- Clostridium botulinum
- Clostridium perfiengens
- Staphylococcus aureus
- Listeria monocytogens
2. Pediococcus pentosaceus Lactic Acid Bacteria [4.0x107 cfu/g]
 produce Lactic acid and Pediocins
 inhibit growth of gram negative bacteria as: Vibrio cholera, E. coli, Salmonella sp.
etc.
 and inhibit growth of gram positive bacteria as: Clostridium botulinum,
Clostridium perfiengens, Staphylococcus aureus etc.
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Mode of Action
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Digest
Organic
mater
Enzymes
- Lipase
- Amylase
- Protease
- Peptidase
- Sucrase
3. Bacillus amyloliquefaciens [6.2x107 cfu/g]
Bacteria that produces Iturins (Antifungal Agent) to inhibit growth of
fungi as: Aspergillus, Fusarium, Candida, Phytopthera Collectotricum,
Rhizoctonia etc. and produces enzymes as: Amylase, Lipase, Protease,
Peptidase, Sucrase
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Produce
Toxins
Killer
Aflatoxins
Mycotoxins
Endotoxins
4. Pichia farinosa [8.2x107 cfu/g]
Rarely yeast found in the nature, produces Toxins Killer to
inhibit the growth of yeasts and neutralizes toxins as:
aflatoxins, mycotoxins and endotoxins
Denature
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Ferment
Starch
Sugar
Acetic acid
- Cellulase
- Hemicellulase
- Xylanase
- Cellubioase
- Amylase
- Pectinase
- Lignase
- Arabinase
5. Dekkera anomala [7.0x107 cfu/g]
Spoilage Beer Yeast- ferments carbohydrate, starch and sugar
into Acetic Acid without alcohol and produces enzymes as:
Cellulase, Cellubioase, Pectinase, Lignase, Amylase,
Hemicellulase and Xylanase
Enzymes
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Work effectively in..
Eliminate unpleasant odor
Reduce and inhibit harmful bacteria
Digest organic matters effectively
Toxins killer (Detoxification)
Truly Natural
Save Energy
Chemical free
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Bioway working parameters
Parameter Range Benefit
Alkalinity pH 1.5 – 9.6 Wide range of operative
conditions
Oxygen Content 0.5ppm + Facultative Anaerobe /
Aerobic
Salinity Up to 200,000 ppm Effective in Saline
conditions
Temperature 0-65°C Wide temperature range
Chlorine Up to 3ppm Operative in moderate
chlorine
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• Japan Food Research Center Foundation:
Tested on toxicity level (LD50). There were no abnormal symptom to any essential organs and Zero
dead animal, the 21,000 mg. per kilogram of weight has been fed. (LD50 limit > 21,000 mg/kg BW)
• Thailand Institute of Scientific and Technological Research (TISTR) :
Tested on the Acute Oral Toxicity Level (LD50). After ad libitum dosing 14 days, all rats appear normal
and zero mortality. Necropsy finding at the termination showed no gross pathological lesions.
• National Institute of Health, Department of Medical Sciences, Ministry of Public Health of
Thailand:
Detection and Isolation of Microorganisms > result > Pathogenic Bacteria and Fungi were not found.
• Overseas Merchandise Inspection Company :
Tests show bactericide effect against the prevention and growth of Salmonella bacteria
• Neotron and LCFA :
Test result of Aflatoxins and Insecticides contamination
• Laboratory Biosafety Manual, Global Health Security, World Health Organization, Geneva 2003 :
This group classification is BIO SAFETY LEVEL 1 (no or very low individual and community risk)
Product Safety
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ANIMAL HUSBANDRY
(poultry, swine & ruminant farms)
• Reduces death & intestinal diseases
• Treat food poisoning diarrhea
• Eliminates bad odor from manure, waste water &
garbage
Existing Problems
Diarrhea & intestinal pain from food poisoning bacteria:
Clostridium botulinum Clostridium perfringens
Vibrio cholerae Vibro parahaemolyticus
Staphylococcus aureus Escherichia coli
Salmonella typhi Listeria monocytogens
Campylobacter Jejuni/coli Aeromonas hydrophila
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Benefit
• Replace using of Antibiotics, Toxins Binders, Yacca,
Enzymes, Insecticides etc.
• Reduce Feces, Better Digestion & Absorption
• Increase Protein, Carbohydrate, Fat Digestibility
• Better FCR & ADG, Increase sexual ability
• Reduces death & GI diseases especially chicks, weaning piglets,
veal
• Treat food poisoning bacteria.
• Eliminates bad odor from manure and waste water &
Speed up decay process
• Decrease houseflies, Control flies & mosquitoes hatch
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• Increase Methane in Biogas tank
• Improve surrounding for animals and neighbors
• Decrease cost, More Profit
• Safe for users, consumers
• Minimize chemical usage
• Decrease water BOD, COD, Turbidity
• Control blooming of Blue Green Algae
• Reduce accumulate of toxic substances in soil
and water
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Reduce Urine Smell, Livestock’s house
By mix with feed:
• use 0.5-1 kg/ton feed
Or mix in water spray
hang 1 kg in 20 tons
water tank every
week
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Control Diarrhea, Intestinal pain from food
poisoning bacteria
Apply 1 gm / 10 kg body weight.
Diarrhea will stop within 15 minutes.
Better healthy, significantly decrease urine
& feces smell and prevent diarrhea &
intestine diseases dead.
mix 0.5 gm / 10 kg body weight
(50 ppm) with feed daily.
ANIMAL FARM
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Manure no smell in Livestock’s house
Poultry farms
Swine Farms
Ruminant Farms
control hatching of
housefly eggs,
larva, chrysalis &
speed up the
decay process
By mixing with feed:
• use 500-1,000 gm/ton feed
• use 1,000-2,000 gm/ton feed for extra security
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Tested Result- Biogas
Pig Manure Substrate in Anaerobic Digester Units:
(4 tested units, 4 control units)
Biogas Increase Up to Decrease Down to
CH4 35-40% 60-65% - -
NH3 - - 2-3% 0.1%
H2S - - 0.5-1.0% 0.01%
CO2 - - 50-55% 30-35%
Bio-gas Production
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Proven Results:
Smell Reduced (90%) after 15 minutes
Mix 1 kg in 3,000 liter of water
pour or spray cover 100 cubic meters waste volume
or 400-1000 square meters space every 3-7 days.
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Waste water from farm
Use 2 kg. per 1,000 cubic meters, by hanging 1 kg BIOWAY at the
water inlet. Change every month.
Before After
Reduce BOD in water BOD (Biological Oxygen Demand)
BOD more organic waste and more
bacteria in water, more oxygen needed,
more electricity needed = waste water
BODorganic waste has been
eliminated and less oxygen needed
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Air Quality Emission
Benefits
1. Rapid reduction of odor emissions “Green air quality”
2. Elimination of odors molecules upon contact
3. Effective on wide range of VOC’s
4. Effective in high volume air stream
5. Simple continuous application
6. Decrease scum accumulate in smoke stack and decrease cleansing cost
7. Safe to use
8. Highly cost effective
Eliminate and control wide range of
volatile organic odors including
- mercaptans,
- amines,
- ammonia,
- hydrogen sulfide
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Odor Control – Air Quality Emission
•Dry wind output of 24,000 m3 per hour (900mm diameter, 3 meter stack
long)
•BIOWAY has been applied via a 1,000 psi, low volume misting system into
the stack.
•BIOWAY was mixed into solution and applied at 100 gm into a 200 L
reservoir (500 ppm).
The solution was then delivered via 8 nozzles in spiral
formation along the stack at 6.25 L of solution per
nozzle per hour.
The usage of BIOWAY is 50 gm per hour.
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Results
Independent sampling by SGS was taken on the 3rd of April 2008, prior to
application of Biowish. The following 10 minutes of application further sampling
was taken.
Odor Control – Air Quality Emission
ppm mg/m3
Prior 15.10 34.58
Treated 3.92 (74.0%) 8.98 (74.0%)
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Carbon Credit
Minimize several pollutants e.g. acid rain and nitrous oxide
Clean technologies, clean air quality emission
The Carbon Credit is this new currency and one Carbon
Credit is equal to one Ton of CO2 and is called a CO2e
(CO2 equivalent).
Costs are between US $10 - 40 per credit.
ภัทรา ศรีสุทธิวรกุล Research 3 สถาบันสิ่งแวดล้อมไทย
โทร 02-5033333 ต่อ 518 โทรสาร 02-5044826-8 e-mail: pattra@tei.or.th
Kyoto Protocol 2004 Air quality emission
Lowering carbon footprint on a voluntary basis
= measure of the impact human activities have
on the environment in terms of the amount
of greenhouse gases produced, measured
in units of carbon dioxide
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CPF- Sriracha, Chonburi
Wet Scrubber Water Quality Improvement
Before using BIOWAY enzymes and microbes
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Odor control and waste water in Wet Scrubber no.1 at CPF–
Sriracha, Chonburi, on May 2008 by added BIOWAY.
Wet Scrubber no. 2 had not been added, to be used for
compare the result.
-Dilute 1,000 grams BIOWAY into 50 L water
-Pour into the wet scrubber 80 tons
-Stir with stick for mixed enzymes and microbes with water
inside the pond
Odor Control – Wet Scrubber No.1
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Odor Control – Wet Scrubber No.1 & 2
Wet
Scrubber
no.1
Water
Color after
using
BIOWAY
Wet Scrubber no.2
Water Color
Results
After One month, the water taken out from the wet scrubber no.1 &
no. 2. By visual observation, the water color in no.1 is more
clear, less polluted and much better in unpleasant odor
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Water Scrubber Treatment
CHAROEN POKPHAND FEEDMILL
(CP Group) – Bangkok
• 80,000 L Water Scrubber
• BIOWAY added to reservoir
• Dosage 50ppm
• Water sample taken after 30
days
• Extended water life
• Reduced odour &
environmental emissions.
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Water treatment, Generate fresh clean air
Cooling tower system
Solve problem in cooling tower system:
- Eliminate blue green algae
- Inhibit Legionaire & Shigella Fungi Contamination
- Digest Bio-film sticker on pipeline, AHU system
- Eliminate unpleasant smell in central system
- Increase low temperature exchange and more cooler air flow by
decreasing shield guard from Bio-film
- Decrease electricity power usage sustainable
Inhibit Legionaire & Shigella Fungi
Contamination in cooling system which
cause of respiratory diseases
Certify from clean fresh air in building
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- Effective at very low dosages
- Proven ability to rapidly reduce:
- BOD (Biological Oxygen Demand)
- COD (Chemical Oxygen Demand),
- TDS (Total Dissolved Solids)
- Turbidity & SS (Suspended Solids)
- TKN (Total Nitrogen, including Ammonia, Nitrate, Nitrite)
- Phosphorous
- Oils & Grease,
- Faecal Coliforms
- Many chemical pollutants
- Removes Blue Green Algae
Water Treatment Outcomes
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BIOWAY™ Enhancement
Enzymatic
Efficiency
Microbial
Load
Organic
Load
Dissolved
O2
Aeration
Requiremen
t
Sludge
Volume
Current
Operations
Low High Slow
Removal
Low High High
BIOWAY™
Enhanceme
nt
Very High Low Rapid
Removal
High Low Low
BIOWAY™ improves WWTP operations across many key parameters through a
substantial improvement in enzymatic efficiency
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WWTP Potential Results
Typical expected results from Industrial/sewage treatment
• Increase Dissolved Oxygen (DO) from 2ppm to 4-6ppm
• Reduce electricity consumption for aeration of 50-60%
• Sludge reduction 80-90%
• No requirement for downstream disinfection/sanitisation
• Anaerobic – 70-80% reduction in BOD5 in 3 days
• Aerobic – 90-99% reduction in BOD5 in 3 days
• Simplified operations
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Key Benefits:
1. Increased operational stability, more predicable results,
simplified plant operations
2. Improved Environmental outcomes
3. Reduced operating costs
4. Capacity increases / Potential reduced capex
5. Suitable for all existing treatment plants and processes
(excluding RO)
Waste Water Treatment
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Improved Operations
• Wide operative conditions of BIOWAY microbes reduce
plant variability
• Odour
• Sanitation
• Speed of catalysis
• Reduced risk of Biomass washout or loss
• Process simplification after implementation
• Predictability ultimately justifies reduced monitoring
• Chemicals management
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Improved Environmental Outcomes
• Odour Control
• Reduced sludge to landfill / incineration
• Bio-solids processing – improved quality (pathogens, herbicides,
pesticides, metals)
• Reduced sludge transportation energy costs
• Reduced electricity usage
• Improved biogas generation
• Reduced chemical usage
• Improved final discharge, (TKN, P)
• Improved emissions from tertiary ponds (NO2, NO3)
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Reduced Operating Costs
• Electricity
• Chemical usage
• Sludge disposal
• Sludge transport
• Labour
• Capex for capacity increases
• Potential Green House Gas credit
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Example – Industrial WWTP
• The following data is from the initial 14 days of dosing BIOWAY™
0
100
200
300
400
500
600
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
mg/l
BOD5 - EQ1 Inffluent
Prior to
BiOWiSH
Post
BiOWiSH
0
200
400
600
800
1000
1200
1400
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
COD - EQ1 Inffluent Prior to
BiOWiSH
Post
BiOWiSH
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Da Phuoc Landfill - Vietnam
• Operated by Vietnam Waste
• 3,500 tons per day MSW
• Leachate pond treatment trial
• Issues:
Odour, Discharge standards
Date 7/09/2008 07/22/2008 8/01/2008 08/21/2008
Temperature (0
C) 28.2 32.4 28.1 30.5
pH 8.18 8.97 9.38 9.04
Conductivity: 8820 6930 4710 2890
COD (mg/l) 3360 1800 920 750
NH3-N (mg/l) x 20 5 30
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Waste water treatment plant
Before treat BIOWAY
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Waste water treatment plant
After treat BIOWAY
Decrease Excess Sludge
Before 42 bags/wk, After 2 bags/wk
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Waste water treatment
Eliminate waste water smell
Eliminate blue green algae
Reduce electricity consumption
and pump maintenance
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Day 1 before add BIOWAY
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1 Week after added BIOWAY
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Grease trap digest in 2 weeks
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Air quality analysis was conducted on garbage fermenting tanks at the On Nut Garbage Disposal
Plant operated by the Department of Public Cleaning of Bangkok Metropolitan Administration.
Sampling was undertaken prior to application of Enzymes and microbes and then again 10
minutes following the application
Odor Control – Garbage and Leachate
Parameter Sampling Method
Hydrogen Sulfide Impinger / Titration
Methyl Mercaptan Sampling Bag / G.C.
Total VOC’s Photo Ionization Detector
Bangkok Metropolitan, Ongnoot Dumping Site
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BIOWAY enzymes and microbes were mixed into solution at 1,000 ppm (100gm to
100L of water) and the solution was sprayed to the surface of the fermentation tank.
Odor Control – Garbage and Leachate
Index % Reduction
Hydrogen Sulfide 97.4%
Methyl Mercaptan 94.0%
Total VOC’s 74.5%
Samples were taken for
measurement of Hydrogen
Sulfide, Ammonia and Odor
Values directly prior to
treatment and at 10 and 40
minutes.
Index Prior to
Treatment
(ppm)
Treated with
BIOWAY (ppm)
Hydrogen Sulfide 12.330 0.319
Methyl Mercaptan 2.01 0.12
Total VOC’s 3,756 956
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Se Jing Shan District Sewage Station in Beijing China by the Chinese National Centre for
Environmental Analysis and Measurements.
The purpose of the sampling was to assess the effectiveness of BIOWAY enzymes and microbes in
reducing odour emissions from both solids and liquid sewage streams through treatment
operations as a potential treatment to address fugitive emissions to surrounding residential areas.
Odor Control – Sewage
Test Sampling
H2S NH3 Odor value
Liquid Waste
  
Prior,
10 min, 40 min
Solid Waste    Prior 40 min
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Odor Control – Sewage
Liquid Stream:
BIOWAY enzymes&microbes was mixed into solution at 500gm to 10L of water.
This solution was poured directly into the liquid waste in a tanker vehicle at a
final dosage of 10 ppm
Sample
H2S (mg/m3) NH3 (mg/m3)
Odor Value
(odor unit / DT)
Before 15.8 35.4 412
10 minutes 11.5 31.1 250
40 minutes 4.49 (71.6%) 6.15 (82.6%) 119 (71.1%)
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Curative and preventive against soil – borne
diseases caused by :
Pseudomonas sp. Pythium sp.
Xanthomonas sp. Rhizoctonia sp.
Erwenia sp. Sclerotium sp.
Phytophthora sp. Alternaria sp.
Pseudospora sp. Fusarium sp.
- Diseases caused by the above on fruit trees,
vegetable, ornamentals, turf grass and field crops
AGRICULTURE
Existing Problems
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• Curative and preventive of soil-borne
plant disease
Volume of area 1 Hectare
Direction for use 2.0 kg mix in 3000 L. water (let it be 12-24
hours and filter water spray to cover all
leaves)
Day Everymonth.
• Soil treatment for seed germination and planting
Volume of area 100 Square meter.
Direction for use 100 g mix in 100 L. water spread cover area
Day Every 15 – 30 days.
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Controlled
points
Using BIOWAY No BIOWAY Remark
Power
consumption
In oxidation
pond
Reduce electricity 30-
50% (switch on the
oxidation pump
reduced from 24 hours
to 12-18 hours, or less)
Electricity
consumption
100% (switch on
the oxidation pump
from 24 hours)
BIOWAY microbes
are facultative-with
oxygen or without
(start with oxidation
to speed up the work)
Chemical
usage
No chemical usage Use chemical BIOWAY Microbes help
reduce chemical
usage and protect
environment
Antibiotic
usage in last
Pond before
releasing out
No antibiotic needed,
e.g. chlorine, phenol
Necessary to use
chlorine, phenol
BIOWAY microbes
produce Pediocins
That have antiseptic
properties.
Maintenance
cost
Reduce maintenance
cost 30-70%
Normal
maintenance cost
Save cost and time
Compare electricity consumption using BIOWAY in Waster
Water Treatment
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Compare electricity consumption using Biowish in Waster
Water Treatment
Controlled
points
Using BIOWAY No BIOWAY Remark
Grease pump
out cost
No need to take out grease
floating on the surface and
safe cost in pumping grease
/ sludge out if use the
product continuously
Regularly need to take
out grease floating on
the surface and safe cost
in pumping grease /
sludge out
BIOWAY microbes can
effectively digest grease
and fat. If use
continuously, problem of
excess grease and oil will
fade away.
Technical
System
controlling
No technical technique or
expert needed to take care
of the system
Need support in term of
technical technique or
expert to take care of
the system
Budgeting 1st month: product cost
around 70% of the normal
electricity consumption
Following months: product
cost around 30-50% of the
normal electricity
consumption.
Electricity expense reduces
30-50% of normal monthly
expense. No chemical
required.
Manpower 1 person
required
Electricity cost: 100%
monthly expense
Added chemicals: 50%
of monthly electricity
cost
Manpower: require 2-3
persons
These expenses are
estimated from the
quantity of waste water
flow into the system in
cubic meters/day. The
variation is possible
following the practical
system, oxidation pump,
type of chemicals use,
professional fee and
number of manpower
needed.
Biological help for the human race
CHARACTERISTICS BIOWAY LOCAL EM Bacillus subtilis
Content and purity
Purely 5 strains of
Micro Organism
Undetectable
Only one species of
Bacillus sp
Formulation
Alive in Dry cell
form
Spore forming Spore Forming
Declarable
At least
1 X108 cfu/gm
Can not declared, normally
lower than
1 X105 cfu/gm
Takes 2 days of
Incubation and
normally found at
1 X106-7cfu/gm
Mutation possibility
Least possibility of
mutation
Extremely high mutation
because of many steps
of locally incubation without
reliable process
Moderate mutant
occurring by locally
incubation
Storage and handling
condition
Under room
temperature and
conditions,
guarantee shelf
Life is 3 years
Shorter shelf life and
Recommend under dark
condition only
Under room
temperature and
conditions, guarantee
shelf life is lower than
2 years
Effectiveness
Recognized
by many
organization
on high and
superior efficacy
Tend to decline and
suspected continuous
because of locally incubation
Tend to decline and
suspected continuous
because of locally
incubation
Comparison between Biowish and
others (1)
Biological help for the human race
Comparison between Biowish and
others (2)
CHARACTERISTICS BIOWAY LOCAL EM Bacillus subtilis
TOLERANCE pH
Wider at
1. 5 – 9.6
Preferred alkaline condition Alkaline only
Temperature
Wider at
0 – 65° C
Dysfunction upper 45° C Dysfunction upper 45° C
Salinity
Wider at
0 – 200 ppt
Unable to figure out because
of variable content
Shorter at 0–15 ppt
Antibiotic
Up to 50 ppm of
Antibiotic
substances
Unable to figure out because
of variable content
Lower than 30 ppm in
general
Copper Up to 5 ppm
Unable to figure out because
of variable content
Lower than 5 ppm in
general
Cost of application
Cheaper per
applications and
results
Cheaper per unit price but
high cost for application and
investment for efficacy
Higher in all aspects of
comparison

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Bioway full eng.

  • 1. Biological help for the human race
  • 2. Biological help for the human race BiOWay™ Super Microbe Functionality Microbe Description Function Pediococcus acidilactici Produces Lactic acid & Pediocins to: inhibit growth of gram negative bacteria; Vibrio cholerae, Salmonella sp., Pseudomonas sp., E.coli, Clamphylobacter etc. inhibit growth of gram positive bacteria: Clostridium botulinum,Clostridium perfringens,Staphylococcus aureus, Listeria monocytogens Pediococcus pentosaceus Produces Lactic acid & Pediocins to: inhibit growth of gram negative bacteria: Vibrio cholerae, Salmonella sp., Pseudomonas sp., E.coli, Clamphylobacter etc. and inhibit growth of gram positive bacteria as: Clostridium botulinum, Clostridium perfringens, Staphylococcus aureus, Listeria monocytogens Bacillus amyloliquefaciens Produces Enzymes to: break organic matter into smaller molecules: Amylase, Lipase, Protease, Peptidase, Sucrase Produces: Iturins (Antifungal) to inhibit growth of fungi including: Fusarium, Collectotricum, Rhizoctonia, Aspergillus, Phytopthera Pichia farinosa Yeast rarely found in nature creates Toxins Killer to inhibit the growth of yeasts and neutralizes a wide range of toxins: Aflatoxins, Mycotoxins, Endotoxins, Exotoxins etc Dekkera anomala Spoilage Beer Yeast: ferments carbohydrate, starch and sugar into Acetic acid without alcohol and produces enzymes: Cellulase, Hemicellulase, Xylanase, Cellubioase, Amylase, Pectinase, Lignase, Arabinase etc
  • 3. Biological help for the human race Man use many kinds of chemical in daily life Putting our health and live into Danger Environment becomes toxic
  • 4. Biological help for the human race “BIOWAY™ Enzymes and Microbes” Effective Bio-products from Nature Researched, Developed and Manufactured In Thailand
  • 5. Biological help for the human race MANGROVES & BIOWAY MICROBES
  • 6. Biological help for the human race What is BIOWAY™? BIOWAY™ is a revolutionary combination of human and environmentally friendly active micro-organisms creating a powerful cocktail of beneficial enzymes, super catalyzing enzymes and co-factors with remarkable ability to fully digest a wide range of compounds and sanitize the environment. It is a natural 100% organic, bio-degradable culture without harsh chemicals or residues. BIOWAY™ rapidly digests fats, protein, carbohydrate, cellulose, and a wide range of pollutants leaving the environment in natural balance.
  • 7. Biological help for the human race The power of BIOWAY™ is a newly discovered naturally occurring enzyme known as ‘Super Catalase’. This cultured protein creates a Kinetic Rate Increase for all other enzymes of 1,000’s to 100,000’s of thousands of times their normal rate of catalysis. For example: This ability to enhance natural biochemical reaction speed makes BIOWAY effective in many applications where previously no solution existed. Combined with the broad spectrum enzyme formulation, Pediocins, Iturins and toxins killer, our Kinetic Rate Enhancement makes BIOWAY products much more effective than any other technology in a wide rage of applications. Enzyme Type Natural Kinetic Rate BIOWAY Rate Enhancement Lipase 5,000 / sec 10M / sec Protease 25-30K / sec 1 Billion+ / sec The World’s Most Powerful Enzyme Technology
  • 8. Biological help for the human race Rapidly Degrade & Fully Digest Non-Living Organic Matter Anti-Microbial Anti-Fungal Neutralises Toxins Core Capabilities
  • 9. Biological help for the human race Applications Environmental Odor Control Waste Water Treatment Waste Management Soil Remediation Ground Water Remediation Biogas Production Agricultural Animal Health / Feed Supplement Cropping Cleaning Cleaning / Grease Removal Air-conditioning Cooling Towers Cosmetic Soap, Shampoo, Deodorant, Facial Exfoliator, Body Firming Lotion Consumer Products Compost Aid, Bin Wash, Septic Aid, Waste Digester, Pond & Tank Treatment, Drain Cleaner Human Health Pharmacological, Health Supplements BIOWAY™ is currently used in a broad range of applications:
  • 10. Biological help for the human race BIOWAY™’ is superior performance across its many applications can be summarised as follows: 1. Very High Speed ‘Super Catalase’ the proprietary enzyme in BIOWAY™ makes products formulated with our technology thousands of times faster than what was previously possible. 2. Broad Functionality BIOWAY™’s 4 core capabilities of rapidly breaking down organic waste, anti-microbial, anti-fungal and toxins neutralisation, with the speed enhancement makes BIOWAY™ a truly unique technology. 3. Broad Spectrum BIOWAY™ is formulated to created a very diverse group of catabolic hydrolysing enzymes. This provides an ability to rapidly and fully digest all non-living matter that is organic in origin. 4. Broad Operative Range BIOWAY™ due to its kinetic rate enhancement technology is able to operate in a much broader range of environmental conditions than previously available technologies. What Makes BIOWAY™ so unique?
  • 11. Biological help for the human race Consists of group of Beneficial Microorganisms Highly concentrate, Effective Acting Mainly 5 microorganisms 3 Bacterias 2 Yeasts
  • 12. Biological help for the human race BIOWAY™ Microbe Functionality Microbe Description Function Pediococcus acidilactici Produces Lactic acid & Pediocins to: inhibit growth of gram negative bacteria; Vibrio cholerae, Salmonella sp., Pseudomonas sp., E.coli, Clamphylobacter etc. inhibit growth of gram positive bacteria: Clostridium botulinum, Clostridium perfringens, Staphylococcus aureus, Listeria monocytogens Pediococcus pentosaceus Produces Lactic acid & Pediocins to: inhibit growth of gram negative bacteria: Vibrio cholerae, Salmonella sp., Pseudomonas sp., E.coli, Clamphylobacter etc. and inhibit growth of gram positive bacteria as: Clostridium botulinum, Clostridium perfringens, Staphylococcus aureus, Listeria monocytogens Bacillus amyloliquefaciens Produces Enzymes to: break organic matter into smaller molecules: Amylase, Lipase, Protease, Peptidase, Sucrase Produces: Iturins (Antifungal) to inhibit growth of fungi including: Fusarium, Collectotricum, Rhizoctonia, Alternaria, Aspergillus, Phytopthera Pichia farinosa Yeast rarely found in nature creates Toxins Killer to inhibit the growth of yeasts and neutralizes a wide range of toxins: Aflatoxins, Mycotoxins, Endotoxins, etc Dekkera anomala Spoilage Beer Yeast: ferments carbohydrate, starch and sugar into Acetic acid without alcohol and produces enzymes: Cellulase, Hemicellulase, Xylanase, Cellubioase, Amylase, Pectinase, Lignase, Arabinase etc
  • 13. Biological help for the human race Digest Organic matter Lactic acid Bacteriocin (Pediocins) Gram-negative - Salmonella - E. coli - Pseudomonas - Vibrio cholerae Gram-Positive - Clostridium botulinum - Clostridium perfiengens - Staphylococcus aureus - Listeria monocytogens 1. Pediococcus acidilactici Lactic Acid Bacteria [5.0x107 cfu/g]  produce Lactic acid and Pediocins  inhibit growth of gram negative bacteria as: Vibrio cholerae, E. coli, Salmonella sp. etc.  and inhibit growth of gram positive bacteria as: Clostridium botulinum, Clostridium perfiengens, Staphylococcus aureus etc.
  • 14. Biological help for the human race Digest Organic Matter Lactic acid Bacteriocin (Pediocins) Gram-negative - Salmonella - E. coli - Pseudomonas - Vibrio cholerae Gram-Positive - Clostridium botulinum - Clostridium perfiengens - Staphylococcus aureus - Listeria monocytogens 2. Pediococcus pentosaceus Lactic Acid Bacteria [4.0x107 cfu/g]  produce Lactic acid and Pediocins  inhibit growth of gram negative bacteria as: Vibrio cholera, E. coli, Salmonella sp. etc.  and inhibit growth of gram positive bacteria as: Clostridium botulinum, Clostridium perfiengens, Staphylococcus aureus etc.
  • 15. Biological help for the human race Mode of Action
  • 16. Biological help for the human race Digest Organic mater Enzymes - Lipase - Amylase - Protease - Peptidase - Sucrase 3. Bacillus amyloliquefaciens [6.2x107 cfu/g] Bacteria that produces Iturins (Antifungal Agent) to inhibit growth of fungi as: Aspergillus, Fusarium, Candida, Phytopthera Collectotricum, Rhizoctonia etc. and produces enzymes as: Amylase, Lipase, Protease, Peptidase, Sucrase
  • 17. Biological help for the human race Produce Toxins Killer Aflatoxins Mycotoxins Endotoxins 4. Pichia farinosa [8.2x107 cfu/g] Rarely yeast found in the nature, produces Toxins Killer to inhibit the growth of yeasts and neutralizes toxins as: aflatoxins, mycotoxins and endotoxins Denature
  • 18. Biological help for the human race Ferment Starch Sugar Acetic acid - Cellulase - Hemicellulase - Xylanase - Cellubioase - Amylase - Pectinase - Lignase - Arabinase 5. Dekkera anomala [7.0x107 cfu/g] Spoilage Beer Yeast- ferments carbohydrate, starch and sugar into Acetic Acid without alcohol and produces enzymes as: Cellulase, Cellubioase, Pectinase, Lignase, Amylase, Hemicellulase and Xylanase Enzymes
  • 19. Biological help for the human race Work effectively in.. Eliminate unpleasant odor Reduce and inhibit harmful bacteria Digest organic matters effectively Toxins killer (Detoxification) Truly Natural Save Energy Chemical free
  • 20. Biological help for the human race Bioway working parameters Parameter Range Benefit Alkalinity pH 1.5 – 9.6 Wide range of operative conditions Oxygen Content 0.5ppm + Facultative Anaerobe / Aerobic Salinity Up to 200,000 ppm Effective in Saline conditions Temperature 0-65°C Wide temperature range Chlorine Up to 3ppm Operative in moderate chlorine
  • 21. Biological help for the human race • Japan Food Research Center Foundation: Tested on toxicity level (LD50). There were no abnormal symptom to any essential organs and Zero dead animal, the 21,000 mg. per kilogram of weight has been fed. (LD50 limit > 21,000 mg/kg BW) • Thailand Institute of Scientific and Technological Research (TISTR) : Tested on the Acute Oral Toxicity Level (LD50). After ad libitum dosing 14 days, all rats appear normal and zero mortality. Necropsy finding at the termination showed no gross pathological lesions. • National Institute of Health, Department of Medical Sciences, Ministry of Public Health of Thailand: Detection and Isolation of Microorganisms > result > Pathogenic Bacteria and Fungi were not found. • Overseas Merchandise Inspection Company : Tests show bactericide effect against the prevention and growth of Salmonella bacteria • Neotron and LCFA : Test result of Aflatoxins and Insecticides contamination • Laboratory Biosafety Manual, Global Health Security, World Health Organization, Geneva 2003 : This group classification is BIO SAFETY LEVEL 1 (no or very low individual and community risk) Product Safety
  • 22. Biological help for the human race ANIMAL HUSBANDRY (poultry, swine & ruminant farms) • Reduces death & intestinal diseases • Treat food poisoning diarrhea • Eliminates bad odor from manure, waste water & garbage Existing Problems Diarrhea & intestinal pain from food poisoning bacteria: Clostridium botulinum Clostridium perfringens Vibrio cholerae Vibro parahaemolyticus Staphylococcus aureus Escherichia coli Salmonella typhi Listeria monocytogens Campylobacter Jejuni/coli Aeromonas hydrophila
  • 23. Biological help for the human race Benefit • Replace using of Antibiotics, Toxins Binders, Yacca, Enzymes, Insecticides etc. • Reduce Feces, Better Digestion & Absorption • Increase Protein, Carbohydrate, Fat Digestibility • Better FCR & ADG, Increase sexual ability • Reduces death & GI diseases especially chicks, weaning piglets, veal • Treat food poisoning bacteria. • Eliminates bad odor from manure and waste water & Speed up decay process • Decrease houseflies, Control flies & mosquitoes hatch
  • 24. Biological help for the human race • Increase Methane in Biogas tank • Improve surrounding for animals and neighbors • Decrease cost, More Profit • Safe for users, consumers • Minimize chemical usage • Decrease water BOD, COD, Turbidity • Control blooming of Blue Green Algae • Reduce accumulate of toxic substances in soil and water
  • 25. Biological help for the human race Reduce Urine Smell, Livestock’s house By mix with feed: • use 0.5-1 kg/ton feed Or mix in water spray hang 1 kg in 20 tons water tank every week
  • 26. Biological help for the human race Control Diarrhea, Intestinal pain from food poisoning bacteria Apply 1 gm / 10 kg body weight. Diarrhea will stop within 15 minutes. Better healthy, significantly decrease urine & feces smell and prevent diarrhea & intestine diseases dead. mix 0.5 gm / 10 kg body weight (50 ppm) with feed daily. ANIMAL FARM
  • 27. Biological help for the human race Manure no smell in Livestock’s house Poultry farms Swine Farms Ruminant Farms control hatching of housefly eggs, larva, chrysalis & speed up the decay process By mixing with feed: • use 500-1,000 gm/ton feed • use 1,000-2,000 gm/ton feed for extra security
  • 28. Biological help for the human race Tested Result- Biogas Pig Manure Substrate in Anaerobic Digester Units: (4 tested units, 4 control units) Biogas Increase Up to Decrease Down to CH4 35-40% 60-65% - - NH3 - - 2-3% 0.1% H2S - - 0.5-1.0% 0.01% CO2 - - 50-55% 30-35% Bio-gas Production
  • 29. Biological help for the human race Proven Results: Smell Reduced (90%) after 15 minutes Mix 1 kg in 3,000 liter of water pour or spray cover 100 cubic meters waste volume or 400-1000 square meters space every 3-7 days.
  • 30. Biological help for the human race Waste water from farm Use 2 kg. per 1,000 cubic meters, by hanging 1 kg BIOWAY at the water inlet. Change every month. Before After Reduce BOD in water BOD (Biological Oxygen Demand) BOD more organic waste and more bacteria in water, more oxygen needed, more electricity needed = waste water BODorganic waste has been eliminated and less oxygen needed
  • 31. Biological help for the human race Air Quality Emission Benefits 1. Rapid reduction of odor emissions “Green air quality” 2. Elimination of odors molecules upon contact 3. Effective on wide range of VOC’s 4. Effective in high volume air stream 5. Simple continuous application 6. Decrease scum accumulate in smoke stack and decrease cleansing cost 7. Safe to use 8. Highly cost effective Eliminate and control wide range of volatile organic odors including - mercaptans, - amines, - ammonia, - hydrogen sulfide
  • 32. Biological help for the human race Odor Control – Air Quality Emission •Dry wind output of 24,000 m3 per hour (900mm diameter, 3 meter stack long) •BIOWAY has been applied via a 1,000 psi, low volume misting system into the stack. •BIOWAY was mixed into solution and applied at 100 gm into a 200 L reservoir (500 ppm). The solution was then delivered via 8 nozzles in spiral formation along the stack at 6.25 L of solution per nozzle per hour. The usage of BIOWAY is 50 gm per hour.
  • 33. Biological help for the human race Results Independent sampling by SGS was taken on the 3rd of April 2008, prior to application of Biowish. The following 10 minutes of application further sampling was taken. Odor Control – Air Quality Emission ppm mg/m3 Prior 15.10 34.58 Treated 3.92 (74.0%) 8.98 (74.0%)
  • 34. Biological help for the human race Carbon Credit Minimize several pollutants e.g. acid rain and nitrous oxide Clean technologies, clean air quality emission The Carbon Credit is this new currency and one Carbon Credit is equal to one Ton of CO2 and is called a CO2e (CO2 equivalent). Costs are between US $10 - 40 per credit. ภัทรา ศรีสุทธิวรกุล Research 3 สถาบันสิ่งแวดล้อมไทย โทร 02-5033333 ต่อ 518 โทรสาร 02-5044826-8 e-mail: pattra@tei.or.th Kyoto Protocol 2004 Air quality emission Lowering carbon footprint on a voluntary basis = measure of the impact human activities have on the environment in terms of the amount of greenhouse gases produced, measured in units of carbon dioxide
  • 35. Biological help for the human race CPF- Sriracha, Chonburi Wet Scrubber Water Quality Improvement Before using BIOWAY enzymes and microbes
  • 36. Biological help for the human race Odor control and waste water in Wet Scrubber no.1 at CPF– Sriracha, Chonburi, on May 2008 by added BIOWAY. Wet Scrubber no. 2 had not been added, to be used for compare the result. -Dilute 1,000 grams BIOWAY into 50 L water -Pour into the wet scrubber 80 tons -Stir with stick for mixed enzymes and microbes with water inside the pond Odor Control – Wet Scrubber No.1
  • 37. Biological help for the human race Odor Control – Wet Scrubber No.1 & 2 Wet Scrubber no.1 Water Color after using BIOWAY Wet Scrubber no.2 Water Color Results After One month, the water taken out from the wet scrubber no.1 & no. 2. By visual observation, the water color in no.1 is more clear, less polluted and much better in unpleasant odor
  • 38. Biological help for the human race Water Scrubber Treatment CHAROEN POKPHAND FEEDMILL (CP Group) – Bangkok • 80,000 L Water Scrubber • BIOWAY added to reservoir • Dosage 50ppm • Water sample taken after 30 days • Extended water life • Reduced odour & environmental emissions.
  • 39. Biological help for the human race Water treatment, Generate fresh clean air Cooling tower system Solve problem in cooling tower system: - Eliminate blue green algae - Inhibit Legionaire & Shigella Fungi Contamination - Digest Bio-film sticker on pipeline, AHU system - Eliminate unpleasant smell in central system - Increase low temperature exchange and more cooler air flow by decreasing shield guard from Bio-film - Decrease electricity power usage sustainable Inhibit Legionaire & Shigella Fungi Contamination in cooling system which cause of respiratory diseases Certify from clean fresh air in building
  • 40. Biological help for the human race - Effective at very low dosages - Proven ability to rapidly reduce: - BOD (Biological Oxygen Demand) - COD (Chemical Oxygen Demand), - TDS (Total Dissolved Solids) - Turbidity & SS (Suspended Solids) - TKN (Total Nitrogen, including Ammonia, Nitrate, Nitrite) - Phosphorous - Oils & Grease, - Faecal Coliforms - Many chemical pollutants - Removes Blue Green Algae Water Treatment Outcomes
  • 41. Biological help for the human race BIOWAY™ Enhancement Enzymatic Efficiency Microbial Load Organic Load Dissolved O2 Aeration Requiremen t Sludge Volume Current Operations Low High Slow Removal Low High High BIOWAY™ Enhanceme nt Very High Low Rapid Removal High Low Low BIOWAY™ improves WWTP operations across many key parameters through a substantial improvement in enzymatic efficiency
  • 42. Biological help for the human race WWTP Potential Results Typical expected results from Industrial/sewage treatment • Increase Dissolved Oxygen (DO) from 2ppm to 4-6ppm • Reduce electricity consumption for aeration of 50-60% • Sludge reduction 80-90% • No requirement for downstream disinfection/sanitisation • Anaerobic – 70-80% reduction in BOD5 in 3 days • Aerobic – 90-99% reduction in BOD5 in 3 days • Simplified operations
  • 43. Biological help for the human race Key Benefits: 1. Increased operational stability, more predicable results, simplified plant operations 2. Improved Environmental outcomes 3. Reduced operating costs 4. Capacity increases / Potential reduced capex 5. Suitable for all existing treatment plants and processes (excluding RO) Waste Water Treatment
  • 44. Biological help for the human race Improved Operations • Wide operative conditions of BIOWAY microbes reduce plant variability • Odour • Sanitation • Speed of catalysis • Reduced risk of Biomass washout or loss • Process simplification after implementation • Predictability ultimately justifies reduced monitoring • Chemicals management
  • 45. Biological help for the human race Improved Environmental Outcomes • Odour Control • Reduced sludge to landfill / incineration • Bio-solids processing – improved quality (pathogens, herbicides, pesticides, metals) • Reduced sludge transportation energy costs • Reduced electricity usage • Improved biogas generation • Reduced chemical usage • Improved final discharge, (TKN, P) • Improved emissions from tertiary ponds (NO2, NO3)
  • 46. Biological help for the human race Reduced Operating Costs • Electricity • Chemical usage • Sludge disposal • Sludge transport • Labour • Capex for capacity increases • Potential Green House Gas credit
  • 47. Biological help for the human race Example – Industrial WWTP • The following data is from the initial 14 days of dosing BIOWAY™ 0 100 200 300 400 500 600 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 mg/l BOD5 - EQ1 Inffluent Prior to BiOWiSH Post BiOWiSH 0 200 400 600 800 1000 1200 1400 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 COD - EQ1 Inffluent Prior to BiOWiSH Post BiOWiSH
  • 48. Biological help for the human race Da Phuoc Landfill - Vietnam • Operated by Vietnam Waste • 3,500 tons per day MSW • Leachate pond treatment trial • Issues: Odour, Discharge standards Date 7/09/2008 07/22/2008 8/01/2008 08/21/2008 Temperature (0 C) 28.2 32.4 28.1 30.5 pH 8.18 8.97 9.38 9.04 Conductivity: 8820 6930 4710 2890 COD (mg/l) 3360 1800 920 750 NH3-N (mg/l) x 20 5 30
  • 49. Biological help for the human race Waste water treatment plant Before treat BIOWAY
  • 50. Biological help for the human race Waste water treatment plant After treat BIOWAY Decrease Excess Sludge Before 42 bags/wk, After 2 bags/wk
  • 51. Biological help for the human race Waste water treatment Eliminate waste water smell Eliminate blue green algae Reduce electricity consumption and pump maintenance
  • 52. Biological help for the human race Day 1 before add BIOWAY
  • 53. Biological help for the human race 1 Week after added BIOWAY
  • 54. Biological help for the human race Grease trap digest in 2 weeks
  • 55. Biological help for the human race Air quality analysis was conducted on garbage fermenting tanks at the On Nut Garbage Disposal Plant operated by the Department of Public Cleaning of Bangkok Metropolitan Administration. Sampling was undertaken prior to application of Enzymes and microbes and then again 10 minutes following the application Odor Control – Garbage and Leachate Parameter Sampling Method Hydrogen Sulfide Impinger / Titration Methyl Mercaptan Sampling Bag / G.C. Total VOC’s Photo Ionization Detector Bangkok Metropolitan, Ongnoot Dumping Site
  • 56. Biological help for the human race BIOWAY enzymes and microbes were mixed into solution at 1,000 ppm (100gm to 100L of water) and the solution was sprayed to the surface of the fermentation tank. Odor Control – Garbage and Leachate Index % Reduction Hydrogen Sulfide 97.4% Methyl Mercaptan 94.0% Total VOC’s 74.5% Samples were taken for measurement of Hydrogen Sulfide, Ammonia and Odor Values directly prior to treatment and at 10 and 40 minutes. Index Prior to Treatment (ppm) Treated with BIOWAY (ppm) Hydrogen Sulfide 12.330 0.319 Methyl Mercaptan 2.01 0.12 Total VOC’s 3,756 956
  • 57. Biological help for the human race Se Jing Shan District Sewage Station in Beijing China by the Chinese National Centre for Environmental Analysis and Measurements. The purpose of the sampling was to assess the effectiveness of BIOWAY enzymes and microbes in reducing odour emissions from both solids and liquid sewage streams through treatment operations as a potential treatment to address fugitive emissions to surrounding residential areas. Odor Control – Sewage Test Sampling H2S NH3 Odor value Liquid Waste    Prior, 10 min, 40 min Solid Waste    Prior 40 min
  • 58. Biological help for the human race Odor Control – Sewage Liquid Stream: BIOWAY enzymes&microbes was mixed into solution at 500gm to 10L of water. This solution was poured directly into the liquid waste in a tanker vehicle at a final dosage of 10 ppm Sample H2S (mg/m3) NH3 (mg/m3) Odor Value (odor unit / DT) Before 15.8 35.4 412 10 minutes 11.5 31.1 250 40 minutes 4.49 (71.6%) 6.15 (82.6%) 119 (71.1%)
  • 59. Biological help for the human race
  • 60. Biological help for the human race Curative and preventive against soil – borne diseases caused by : Pseudomonas sp. Pythium sp. Xanthomonas sp. Rhizoctonia sp. Erwenia sp. Sclerotium sp. Phytophthora sp. Alternaria sp. Pseudospora sp. Fusarium sp. - Diseases caused by the above on fruit trees, vegetable, ornamentals, turf grass and field crops AGRICULTURE Existing Problems
  • 61. Biological help for the human race • Curative and preventive of soil-borne plant disease Volume of area 1 Hectare Direction for use 2.0 kg mix in 3000 L. water (let it be 12-24 hours and filter water spray to cover all leaves) Day Everymonth. • Soil treatment for seed germination and planting Volume of area 100 Square meter. Direction for use 100 g mix in 100 L. water spread cover area Day Every 15 – 30 days.
  • 62. Biological help for the human race Controlled points Using BIOWAY No BIOWAY Remark Power consumption In oxidation pond Reduce electricity 30- 50% (switch on the oxidation pump reduced from 24 hours to 12-18 hours, or less) Electricity consumption 100% (switch on the oxidation pump from 24 hours) BIOWAY microbes are facultative-with oxygen or without (start with oxidation to speed up the work) Chemical usage No chemical usage Use chemical BIOWAY Microbes help reduce chemical usage and protect environment Antibiotic usage in last Pond before releasing out No antibiotic needed, e.g. chlorine, phenol Necessary to use chlorine, phenol BIOWAY microbes produce Pediocins That have antiseptic properties. Maintenance cost Reduce maintenance cost 30-70% Normal maintenance cost Save cost and time Compare electricity consumption using BIOWAY in Waster Water Treatment
  • 63. Biological help for the human race Compare electricity consumption using Biowish in Waster Water Treatment Controlled points Using BIOWAY No BIOWAY Remark Grease pump out cost No need to take out grease floating on the surface and safe cost in pumping grease / sludge out if use the product continuously Regularly need to take out grease floating on the surface and safe cost in pumping grease / sludge out BIOWAY microbes can effectively digest grease and fat. If use continuously, problem of excess grease and oil will fade away. Technical System controlling No technical technique or expert needed to take care of the system Need support in term of technical technique or expert to take care of the system Budgeting 1st month: product cost around 70% of the normal electricity consumption Following months: product cost around 30-50% of the normal electricity consumption. Electricity expense reduces 30-50% of normal monthly expense. No chemical required. Manpower 1 person required Electricity cost: 100% monthly expense Added chemicals: 50% of monthly electricity cost Manpower: require 2-3 persons These expenses are estimated from the quantity of waste water flow into the system in cubic meters/day. The variation is possible following the practical system, oxidation pump, type of chemicals use, professional fee and number of manpower needed.
  • 64. Biological help for the human race CHARACTERISTICS BIOWAY LOCAL EM Bacillus subtilis Content and purity Purely 5 strains of Micro Organism Undetectable Only one species of Bacillus sp Formulation Alive in Dry cell form Spore forming Spore Forming Declarable At least 1 X108 cfu/gm Can not declared, normally lower than 1 X105 cfu/gm Takes 2 days of Incubation and normally found at 1 X106-7cfu/gm Mutation possibility Least possibility of mutation Extremely high mutation because of many steps of locally incubation without reliable process Moderate mutant occurring by locally incubation Storage and handling condition Under room temperature and conditions, guarantee shelf Life is 3 years Shorter shelf life and Recommend under dark condition only Under room temperature and conditions, guarantee shelf life is lower than 2 years Effectiveness Recognized by many organization on high and superior efficacy Tend to decline and suspected continuous because of locally incubation Tend to decline and suspected continuous because of locally incubation Comparison between Biowish and others (1)
  • 65. Biological help for the human race Comparison between Biowish and others (2) CHARACTERISTICS BIOWAY LOCAL EM Bacillus subtilis TOLERANCE pH Wider at 1. 5 – 9.6 Preferred alkaline condition Alkaline only Temperature Wider at 0 – 65° C Dysfunction upper 45° C Dysfunction upper 45° C Salinity Wider at 0 – 200 ppt Unable to figure out because of variable content Shorter at 0–15 ppt Antibiotic Up to 50 ppm of Antibiotic substances Unable to figure out because of variable content Lower than 30 ppm in general Copper Up to 5 ppm Unable to figure out because of variable content Lower than 5 ppm in general Cost of application Cheaper per applications and results Cheaper per unit price but high cost for application and investment for efficacy Higher in all aspects of comparison