1. Technology
• “Potential New
Diagnostics, Prognostics, Therapeutics for
Cancer and Heart Disease via Micro RNA miR-
21” – Shawn Lupold
• microRNA: miR-21 is expressed at increased
levels in many diseases including cancer, heart
disease, etc.
• Overexpression of miR-21 imparts proliferative
and oncologic properties to cells.

2. Business Plan
• KP Diagnostics, Inc. plans to license the
technology from Johns Hopkins Technology
Transfer
• It initially plans to provide contract research
services for cancer diagnostics.
• Its future goals include developing diagnostic
kits for cancer detection.

3. miR-21 and Cancer
The list of diseases in which overexpression of miRNA-21 has been found:
• -Breast Cancer
• -Lung Cancer
• -Colon Cancer
• -Prostate Cancer
• -Pancreatic Cancer
• -Liver Cancer
• -Stomach Cancer
• -Brain Cancer
• -Chronic Lymphocytic Leukemia
• -Diffuse Large B-Cell Lymphoma
• -Hodgkin Lymphoma
• -Heart Disease

4. RT- PCR Diagnostic Testing
• We plan to provide contract tests using real time Reverse Transcriptase –
PCR to quantitate miR-21 in a specific cancer.
• Quantitative by Real time RT-PCR can be performed as relative
measurements, comparing the expression of miR-21 with that of a
presumed constantly expressed reference gene.

5. RT-PCR Diagnostic Testing

6. Microarray
KP diagnostics also plans to invest in DNA microarray technology
for diagnostic testing services.
• Step 1: Isolate miR-21
• Step 2: Reverse Transcriptase – to construct cDNA
• Step3: coupling with fluorescent dyes
• Step 4 : Hybridization
• Step 5: Scanning
• Step 6: Normalization and Analysis

7. References
• http://www.jhttonline.jhu.edu/TechnologyDet
ail.aspx?TechID=BFBCC053-CFAE-454C-9DF3-
BFEB437D0A45&JHURef=C10627
• http://www.tataa.com/files/documents/qpcr_
review.pdf