Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

Europass CV 3PAmarish Sharma 2014-Denmark


Published on

  • Be the first to comment

  • Be the first to like this

Europass CV 3PAmarish Sharma 2014-Denmark

  1. 1. © European Union, 2002-2013 | Page 1 / 3 Amarish Kumar Sharma Norrebrogade 51, st 0207, Copenhagen 2200, Denmark. 0551-650 5550 (India) +45 71 36 92 93 (Copenhagen) amarish.sharma Danish Work Permit ( Person ID: 2913541) Sex : Male | Date of birth: 04/07/1980 | Nationality : Indian PROJECTS & ASSIGNMENTS PROFILE I am a biotech professional having over 6 years of research & academic experience.My experience highlights my strength in drug discovery & development program for diabetes, animal cell culture, in-vitro target focussed assay development & standardization and High Throughput Screening. I was also engaged in University teaching & research extensively. Multi-tasking, Can do attitude, risk taking ability, good communication skill and hard work are my strength. August 2010- October 2014 September’2009-April’2010 Assistant Professor Lovely Professional University, Punjab, INDIA | Research Scientist Biocon Ltd, Bangalore, India | Research Scientist March’ 2008-May’2009 August 2007- January 2008 Connexios Life Sciences, Bangalore, INDIA | Research Intern Nicholas Piramal Research Centre, Mumbai, India | PROFESSIONAL EXPERIENCE ▪ Assay development & Standardization for efficacy (Biosimilars/Bioequivalence studies). ▪ Toxicity studies (Toxicogenomics, ADME/Tox), its standardization/optimization. ▪ High-throughput screening (HTS) of NCE’s(Natural/Synthetic therapeutic molecules) using mammalian cell lines(L6,C2C12,H9C2,3T3-L1,3T3-A31,HepG2,HEK293,CHO-K1). ▪ Target focussed projects handled:  Standardization of the diabetic in-vitro assay model which deals with the comparative study of the efficacy of different Insulin analogues (Bio-similar), through assessing the percentage glucose drop using 3T3-L1 cell line.  Standardization of Mitogenic or cell proliferation assay model for the investigation of Mitogenic property of different Insulin analogues (Bio-similar) on induced diabetic model (hyperglycaemic condition) using 3T3-L1 cell line. Research & Development | Drug discovery program
  2. 2. PROFESSIONAL EDUCATION  Investigated the effect of Hepatocyte Nuclear Factor-4α phosphorylation and its transactivation and assessing AMPK activation in Hepatocytes on Type 2 Diabetes assay model.  Assessed the effect of cationic amphiphilic drugs induced Phospholipidosis and their in-vitro high throughput screening (HTS) in CHO-K1 cell line using the fluorescent-labelled phospholipid probe.  Standardized the bio-assay for the analysis of Nephrotoxicity (ADME/Tox) in HEK-293 cell lines and in-vitro screening of the NCE’s (New Chemical Entities) on induced Type 2 Diabetic assay model.  Investigated the relationship between IMTG (Intramuscular triglycerides) and Insulin resistance in Obese Type 2 Diabetic in-vitro model on C2C12 cell line followed by high throughput screening of new chemical entities for its respective efficacy.  Investigated the effect of HSD11b1 mediated Endoplasmic Reticulum stress (ADME/Tox) on Type 2 Diabetic invitro model using C2C12 cell line.  Studied the expression of CPT1A gene and ECHS1 gene which play a major role in accumulation of fat in the liver and is associated with insulin resistance and type 2 diabetes mellitus followed by high throughput in-vitro screening of new chemical entity for its efficacy.  University teaching of subjects namely Stem Cell Technology & Animal Biotechnology, Administrative responsibilities (Pedagogy development, Training, workshop & seminar organization).  Guided & co-ordinated graduate and post-graduate interns in project work concerned to Heavy metal Bioremediation and Food Bio-preservatives. POST-GRADUATE INTERNSHIP: August 2007 - January 2008 (6 months) Project title: ▪ “Bioactivity guided fractionation and in-vitro screening of MHCO2 isolated from marine bacteria showing Anti-Diabetic Activity ". ▪ Investigated the anti-hyperglycaemic effect of different plant metabolic extracts and extracts derived from marine source for their efficacy in glucose uptake in skeletal muscles through an in-vitro assay model using radio-labelled glucose and L6 cell line (skeletal muscles isolated from Rat),at Department of Biotechnology August 2006-December 2007 Master of Technology (M.Tech) in Biotechnology Bundelkhand University, INDIA July 2002 - July 2006 Bachelor of Technology (B.Tech) in Biotechnology Bundelkhand University, INDIA Research & Development | Drug discovery program
  3. 3. © European Union, 2002-2013 | Page 3 / 3  Expertise in Animal Cell Culture; Cell line Maintenance & Cryopreservation.  Cell line characterization and validation.  Assay development for efficacy (Biosimilars/Bioequivalence studies).  Toxicity studies (Toxicogenomics, ADME/Tox).  High-throughput screening (HTS).  RNA isolation, cDNA preparation and quantitative RT-PCR.  SDS-PAGE, Western blotting, and fluorescent imaging techniques.  Experience in working with radioisotopes.  Handled In-vivo experiments (Islets isolation, Pancreas, Mouse model).  Experience with data analysis and graphing software.  Interpret and report data to the drug discovery teams.  Prepare and maintain accurate laboratory records (SOPs).  Experience in handling HPLC and Mass Spectrometry.  TLC, Column Chromatography, End point Thermo cycler, Agarose gel electrophoresis. PERSONAL SKILLS Mother tongue Hindi Other language(s) UNDERSTANDING SPEAKING WRITING Listening Reading Spoken interaction Spoken production English C1/2 C1/2 C1/2 C1/2 C1/2 Replace with name of language certificate. Enter level if known. Danish A1/2 A1/2 A1/2 A1/2 A1/2 Replace with name of language certificate. Enter level if known. Levels: A1/2: Basic user - B1/2: Independent user - C1/2 Proficient user Common European Framework of Reference for Languages Computer skills ▪ Good command of Microsoft Office™ tools ▪ Good command on statistical tools such as Graph pad prism & PLA, for analysing data. Presentations/Conferences Certifications Memberships  Poster Presentation- "Control of Cataract by increasing the activity of enzyme Polyol Dehydrogenase" : International Conference On Biomedical Research and Therapeutic Medicine, Bundelkhand University, India January 8, 2005;Authors: Amarish Sharma, Saurabh Verma  Poster presentation on “ELISA” in All India Engineering Project Contest, organized by Institute Of Engineering & Technology, Bundelkhand University, Jhansi, INDIA on March 21st, 2005.  Participated in National Sensitization Programme on Biotechnology held at D.D.U. Gorakhpur University, Gorakhpur, U.P, INDIA on 6th March, 2005.  Workshop on Chromatography & Mass Spectrometry-Certificate of Attendance ,Indian Institute of Chromatography and Mass Spectrometry LLP License 100108 March 2014  Summer Training at Post Graduate Department of Microbiology, King George Medical University,Lucknow-226003 License 1374/05 ; DOI: 07.09.05 September 2005  Faculty Development Programme on "Stem Cell Technology”, Lovely Professional University License 3652 June 2013  Faculty Development Programme on “Immunology and Immune-technology, Lovely Professional University License 1615 July 2011  MEMBER OF HELP AGE INTERNATIONAL CHARITABLE TRUST  MEMBER OF INDIAN SOCIETY FOR RADIATION BIOLOGY (Applied) Research & Development | Drug discovery program