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Biological Diversty and its definition
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The aim of this work was to find an alternative to chemical fungicides currently used in the control plant pathogenic fungi Rhizoctoniasolani ,ColletotrichummusaeandFusariumoxysporum,. The antifungal activity of the methanol extracts of six medicinal plants used in native medicine in Sri Lanka is reported.All plant extracts were screened for their fungistatic, fungicidal activities and minimum inhibitory dilution (MID) against above fungi. The media amended with methanol and recommended fungicide for respective fungal strain were consider as negative and positive control respectively.Results showed that radial growth in all the three tested organisms was significantly impaired (p<0.05) by the addition of the extracts in the culture medium used. The test fungi differed in their reaction to the different extracts but on the whole, growth inhibition increased with the concentration of each extract. The most active extracts, shows a marked effect of the 20% methanol extracts from sweet flag with inhibition values of 91%, 86% and 84 % for F. oxysporum,R. solani and C.muceawhereas those from wild basil inhibited the growth of the same pathogens by 89%, 84% and 74%.The results showed minimal inhibitory concentrations (MIC) were 5 % (v/v) for sweet flag and wild basil and 20% (v/v) for all other plant crude extracts. Out of six plants extract screened, wild basil and sweet flag showed more than 80% fungal inhibition after 6 hour immersion and other extracts could not exceed 60% inhibition after any exposure time. The study revealed that methanol crude extract of sweet flag and wild basil exhibit strong fungistatic and fungicidal activities against tested fungi. These results support the potential use of these plant extracts in the management of diseases caused by tested plant pathogenic fungi.
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