Considering the cultures used to inoculate each medium in this laboratory, how many different
microbial types should you expect to see in/on each medium? Which medium was most difficult
for you to transfer from? which medium was most difficult for you to inoculate? Explain your
difficulties. What is the purpose of flaming in the aseptic techniques? Provide 3 (three) reasons
why the use of aseptic techniques is essential when handling microbial cultures. Methoos of
isolation What is a microbial colony? Can a pure culture by prepared from a mixd broth or
mixed agar slant? Explain.
Solution
Answers are :
When the cultures are inoculated onto the surface of the medium, different colonies are
obtained, based on the mother culture we have chosen colonies are obtained. Until unless it is not
contaminated so.
Preparation of agar slants is difficult to transfer, and inoculate, because it needs to place
correct angle while solidifying, less surface area. Though among the medium we can say some
difficult but not so rather difficult. Nutrient broth is also difficult to transfer because more prone
of contamination and determination of the bacterial growth is also difficult.
Purpose of flaming during aseptic transfer :- Flaming causes direct burning of the cultures
samples which stick onto the inoculating loop to avoid from contamination, which technique is
called Incineration i.e., Flame sterilization.
Aseptic techniques are safe transfer of culture onto the fresh medium this is very essential
because :-
To avoid contamination to grow unwanted cultures and spoiling the medium components.
To know the perfect study of the unknown characteristics, contamination shouldn’t be seen .
To avoid infecting the person who handling the sample, some may cause severe infections and
death of the investigator.
To obtain the pure culture techniques to study the characteristics of the cultures.
Microbial colony means, group of the similar cells which shows same characteristic features ,
which obtained on the surface of the solid agar medium. These are varied in shapes and surface
but shows same morphological and physiological characteristics.
Yes, we can prepare pure culture from mixed sample either broth or solid agar, in case of
broth prepare serial dilution of the sample to obtain the pure cultures. In case of the solid slant
prepare quadrant streaking on the fresh agar plate..
Measures of Central Tendency: Mean, Median and Mode
Considering the cultures used to inoculate each medium in this labora.pdf
1. Considering the cultures used to inoculate each medium in this laboratory, how many different
microbial types should you expect to see in/on each medium? Which medium was most difficult
for you to transfer from? which medium was most difficult for you to inoculate? Explain your
difficulties. What is the purpose of flaming in the aseptic techniques? Provide 3 (three) reasons
why the use of aseptic techniques is essential when handling microbial cultures. Methoos of
isolation What is a microbial colony? Can a pure culture by prepared from a mixd broth or
mixed agar slant? Explain.
Solution
Answers are :
When the cultures are inoculated onto the surface of the medium, different colonies are
obtained, based on the mother culture we have chosen colonies are obtained. Until unless it is not
contaminated so.
Preparation of agar slants is difficult to transfer, and inoculate, because it needs to place
correct angle while solidifying, less surface area. Though among the medium we can say some
difficult but not so rather difficult. Nutrient broth is also difficult to transfer because more prone
of contamination and determination of the bacterial growth is also difficult.
Purpose of flaming during aseptic transfer :- Flaming causes direct burning of the cultures
samples which stick onto the inoculating loop to avoid from contamination, which technique is
called Incineration i.e., Flame sterilization.
Aseptic techniques are safe transfer of culture onto the fresh medium this is very essential
because :-
To avoid contamination to grow unwanted cultures and spoiling the medium components.
To know the perfect study of the unknown characteristics, contamination shouldn’t be seen .
To avoid infecting the person who handling the sample, some may cause severe infections and
death of the investigator.
To obtain the pure culture techniques to study the characteristics of the cultures.
Microbial colony means, group of the similar cells which shows same characteristic features ,
which obtained on the surface of the solid agar medium. These are varied in shapes and surface
but shows same morphological and physiological characteristics.
Yes, we can prepare pure culture from mixed sample either broth or solid agar, in case of
broth prepare serial dilution of the sample to obtain the pure cultures. In case of the solid slant
prepare quadrant streaking on the fresh agar plate.
