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Isolation Technique ,[object Object],[object Object],[object Object],[object Object]
Mixed Culture from Raw Poultry
Streak Plate Isolation Principle ,[object Object],[object Object],[object Object],[object Object]
The Goal -Isolated Colonies to Start Pure Cultures
Can an isolated colony be considered pure? ,[object Object],[object Object],[object Object],[object Object],[object Object]
Any special considerations? ,[object Object],[object Object],[object Object],[object Object],[object Object]
Microbes will surprise you each chance they get !
Isolation Requires Aseptic Technique
Isolation Requires Aseptic Technique ,[object Object],[object Object],[object Object],[object Object],[object Object]
Streaking   the Quadrants
Quadrant 1-  Streak with broad narrow strokes in the upper half of the first quarter of the plate.
Incinerate and cool the loop between the quadrants
Quadrant 2 –  Rotate the plate, enter the previous streak mark one or two times and then streak the upper portion of the second quarter of the plate with broad strokes.
Incinerate and cool the loop between the quadrants
Quadrant 3 –  Rotate the plate, enter quadrant 2 one or two times and then streak with shorter more separated strokes from the top of the quadrant to the center.
Incinerate and cool the loop between the quadrants
Quadrant 4 –  Enter quadrant 3 and then streak with broad S-shaped motions through the center of the plate.
Streaking the Quadrants
Isolated Colonies
Culturing and Isolation Techniques ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
Colony Morphology
Inoculation of a Broth Culture ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Compare your inoculated broth tube to the un-inoculated control tube to determine the amount of turbidity.  The more turbid the broth The higher the bacterial count per mL of broth.
Inoculating an Agar Slant ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Streak Plates ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Streak Plates
Pour Plating for Colony Counts ,[object Object],[object Object],[object Object],[object Object]
Pour Plating for Colony Counts ,[object Object],[object Object],[object Object],[object Object]
Calculating CFU from dilution plating results   ,[object Object],[object Object],[object Object],The initial dilution is calculated As follows:  So the initial dilution is  1/10 or 0.1 or 10 -1 .
[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object]
[object Object],Therefore, the “Dilution factor for our example is:  10 6  and the Total Colony Forming Units (CFUs) for this sample is:

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Bls 206 lecture 3