SlideShare a Scribd company logo
1 of 1
Download to read offline
If 0.150 mL of 10^-7.5 dilution yielded an average of 256 colonies/plate, calculate the number of
bacteria per mL of the original culture. Show all calculations.
Solution
The number of colony-forming units (CFU) = [no of cells]/ [volume of dilution x volume of
diluted sample added to the plate]
Now CFU per ml of dilution is 256 colonies divided by 0.150 ml plated = 1706.7 colony-
forming units/ml of dilution.
Dividing this number by the 10-7.5 dilution = 1706.7 / 10-7.5 = 1706.7 x 107.5 colony-forming
units/ml of original culture sample

More Related Content

More from kesav24

Considering the cultures used to inoculate each medium in this labora.pdf
Considering the cultures used to inoculate each medium in this labora.pdfConsidering the cultures used to inoculate each medium in this labora.pdf
Considering the cultures used to inoculate each medium in this labora.pdf
kesav24
 
wo alternative investment proposals are under consideration for a va.pdf
wo alternative investment proposals are under consideration for a va.pdfwo alternative investment proposals are under consideration for a va.pdf
wo alternative investment proposals are under consideration for a va.pdf
kesav24
 
You are searching for new species that are highly tolerant to acidic.pdf
You are searching for new species that are highly tolerant to acidic.pdfYou are searching for new species that are highly tolerant to acidic.pdf
You are searching for new species that are highly tolerant to acidic.pdf
kesav24
 

More from kesav24 (20)

Consider this experiment I have 20-sided die where each face has a .pdf
Consider this experiment I have 20-sided die where each face has a .pdfConsider this experiment I have 20-sided die where each face has a .pdf
Consider this experiment I have 20-sided die where each face has a .pdf
 
congenital malformations. why do you think they occurSolution.pdf
congenital malformations. why do you think they occurSolution.pdfcongenital malformations. why do you think they occurSolution.pdf
congenital malformations. why do you think they occurSolution.pdf
 
Considering the cultures used to inoculate each medium in this labora.pdf
Considering the cultures used to inoculate each medium in this labora.pdfConsidering the cultures used to inoculate each medium in this labora.pdf
Considering the cultures used to inoculate each medium in this labora.pdf
 
Are the transfermations linear Are they an isomorphism WhySol.pdf
Are the transfermations linear Are they an isomorphism WhySol.pdfAre the transfermations linear Are they an isomorphism WhySol.pdf
Are the transfermations linear Are they an isomorphism WhySol.pdf
 
bessavillabessavillabessavillabessavillaSoluti.pdf
bessavillabessavillabessavillabessavillaSoluti.pdfbessavillabessavillabessavillabessavillaSoluti.pdf
bessavillabessavillabessavillabessavillaSoluti.pdf
 
ciny Univer Laboratory es and crossing over assortment of independent.pdf
ciny Univer Laboratory es and crossing over assortment of independent.pdfciny Univer Laboratory es and crossing over assortment of independent.pdf
ciny Univer Laboratory es and crossing over assortment of independent.pdf
 
A population of rabbits quadruples every 2 years. If the initial num.pdf
A population of rabbits quadruples every 2 years. If the initial num.pdfA population of rabbits quadruples every 2 years. If the initial num.pdf
A population of rabbits quadruples every 2 years. If the initial num.pdf
 
7. describe and correct the error. Describe and correct the error. .pdf
7. describe and correct the error. Describe and correct the error.  .pdf7. describe and correct the error. Describe and correct the error.  .pdf
7. describe and correct the error. Describe and correct the error. .pdf
 
3. Where would you expect to find fenestrated capillaries (More tha.pdf
3. Where would you expect to find fenestrated capillaries (More tha.pdf3. Where would you expect to find fenestrated capillaries (More tha.pdf
3. Where would you expect to find fenestrated capillaries (More tha.pdf
 
Assembly LanguageReverse an array.Use a loop with indirect or i.pdf
Assembly LanguageReverse an array.Use a loop with indirect or i.pdfAssembly LanguageReverse an array.Use a loop with indirect or i.pdf
Assembly LanguageReverse an array.Use a loop with indirect or i.pdf
 
wo alternative investment proposals are under consideration for a va.pdf
wo alternative investment proposals are under consideration for a va.pdfwo alternative investment proposals are under consideration for a va.pdf
wo alternative investment proposals are under consideration for a va.pdf
 
You are searching for new species that are highly tolerant to acidic.pdf
You are searching for new species that are highly tolerant to acidic.pdfYou are searching for new species that are highly tolerant to acidic.pdf
You are searching for new species that are highly tolerant to acidic.pdf
 
Why must some nutrients be absorbed by active transport rather than .pdf
Why must some nutrients be absorbed by active transport rather than .pdfWhy must some nutrients be absorbed by active transport rather than .pdf
Why must some nutrients be absorbed by active transport rather than .pdf
 
Who first discovered the properties of equalitySolutionEqual.pdf
Who first discovered the properties of equalitySolutionEqual.pdfWho first discovered the properties of equalitySolutionEqual.pdf
Who first discovered the properties of equalitySolutionEqual.pdf
 
What happens when a bacterium is placed in a HYPOTONIC solution Wha.pdf
What happens when a bacterium is placed in a HYPOTONIC solution Wha.pdfWhat happens when a bacterium is placed in a HYPOTONIC solution Wha.pdf
What happens when a bacterium is placed in a HYPOTONIC solution Wha.pdf
 
Why does the stack use a FILO (first in last out) schemeSolutio.pdf
Why does the stack use a FILO (first in last out) schemeSolutio.pdfWhy does the stack use a FILO (first in last out) schemeSolutio.pdf
Why does the stack use a FILO (first in last out) schemeSolutio.pdf
 
Trace the path followed by a sperm and by and egg from the area of t.pdf
Trace the path followed by a sperm and by and egg from the area of t.pdfTrace the path followed by a sperm and by and egg from the area of t.pdf
Trace the path followed by a sperm and by and egg from the area of t.pdf
 
The potential distribution of organism can be influenced by all of th.pdf
The potential distribution of organism can be influenced by all of th.pdfThe potential distribution of organism can be influenced by all of th.pdf
The potential distribution of organism can be influenced by all of th.pdf
 
The function f(x,y)= -x^4-256x+y^3-75y+9 has two critical points. En.pdf
The function f(x,y)= -x^4-256x+y^3-75y+9 has two critical points. En.pdfThe function f(x,y)= -x^4-256x+y^3-75y+9 has two critical points. En.pdf
The function f(x,y)= -x^4-256x+y^3-75y+9 has two critical points. En.pdf
 
1. What are telomeres What distinct mechanisms protect the ends of .pdf
1. What are telomeres What distinct mechanisms protect the ends of .pdf1. What are telomeres What distinct mechanisms protect the ends of .pdf
1. What are telomeres What distinct mechanisms protect the ends of .pdf
 

Recently uploaded

會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文
會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文
會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文
中 央社
 
會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽
會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽
會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽
中 央社
 
SURVEY I created for uni project research
SURVEY I created for uni project researchSURVEY I created for uni project research
SURVEY I created for uni project research
CaitlinCummins3
 

Recently uploaded (20)

Trauma-Informed Leadership - Five Practical Principles
Trauma-Informed Leadership - Five Practical PrinciplesTrauma-Informed Leadership - Five Practical Principles
Trauma-Informed Leadership - Five Practical Principles
 
MOOD STABLIZERS DRUGS.pptx
MOOD     STABLIZERS           DRUGS.pptxMOOD     STABLIZERS           DRUGS.pptx
MOOD STABLIZERS DRUGS.pptx
 
An Overview of the Odoo 17 Knowledge App
An Overview of the Odoo 17 Knowledge AppAn Overview of the Odoo 17 Knowledge App
An Overview of the Odoo 17 Knowledge App
 
Graduate Outcomes Presentation Slides - English (v3).pptx
Graduate Outcomes Presentation Slides - English (v3).pptxGraduate Outcomes Presentation Slides - English (v3).pptx
Graduate Outcomes Presentation Slides - English (v3).pptx
 
會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文
會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文
會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文會考英文
 
diagnosting testing bsc 2nd sem.pptx....
diagnosting testing bsc 2nd sem.pptx....diagnosting testing bsc 2nd sem.pptx....
diagnosting testing bsc 2nd sem.pptx....
 
Supporting Newcomer Multilingual Learners
Supporting Newcomer  Multilingual LearnersSupporting Newcomer  Multilingual Learners
Supporting Newcomer Multilingual Learners
 
Observing-Correct-Grammar-in-Making-Definitions.pptx
Observing-Correct-Grammar-in-Making-Definitions.pptxObserving-Correct-Grammar-in-Making-Definitions.pptx
Observing-Correct-Grammar-in-Making-Definitions.pptx
 
AIM of Education-Teachers Training-2024.ppt
AIM of Education-Teachers Training-2024.pptAIM of Education-Teachers Training-2024.ppt
AIM of Education-Teachers Training-2024.ppt
 
DEMONSTRATION LESSON IN ENGLISH 4 MATATAG CURRICULUM
DEMONSTRATION LESSON IN ENGLISH 4 MATATAG CURRICULUMDEMONSTRATION LESSON IN ENGLISH 4 MATATAG CURRICULUM
DEMONSTRATION LESSON IN ENGLISH 4 MATATAG CURRICULUM
 
male presentation...pdf.................
male presentation...pdf.................male presentation...pdf.................
male presentation...pdf.................
 
Major project report on Tata Motors and its marketing strategies
Major project report on Tata Motors and its marketing strategiesMajor project report on Tata Motors and its marketing strategies
Major project report on Tata Motors and its marketing strategies
 
TỔNG HỢP HƠN 100 ĐỀ THI THỬ TỐT NGHIỆP THPT TOÁN 2024 - TỪ CÁC TRƯỜNG, TRƯỜNG...
TỔNG HỢP HƠN 100 ĐỀ THI THỬ TỐT NGHIỆP THPT TOÁN 2024 - TỪ CÁC TRƯỜNG, TRƯỜNG...TỔNG HỢP HƠN 100 ĐỀ THI THỬ TỐT NGHIỆP THPT TOÁN 2024 - TỪ CÁC TRƯỜNG, TRƯỜNG...
TỔNG HỢP HƠN 100 ĐỀ THI THỬ TỐT NGHIỆP THPT TOÁN 2024 - TỪ CÁC TRƯỜNG, TRƯỜNG...
 
OS-operating systems- ch05 (CPU Scheduling) ...
OS-operating systems- ch05 (CPU Scheduling) ...OS-operating systems- ch05 (CPU Scheduling) ...
OS-operating systems- ch05 (CPU Scheduling) ...
 
Book Review of Run For Your Life Powerpoint
Book Review of Run For Your Life PowerpointBook Review of Run For Your Life Powerpoint
Book Review of Run For Your Life Powerpoint
 
會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽
會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽
會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽會考英聽
 
SURVEY I created for uni project research
SURVEY I created for uni project researchSURVEY I created for uni project research
SURVEY I created for uni project research
 
UChicago CMSC 23320 - The Best Commit Messages of 2024
UChicago CMSC 23320 - The Best Commit Messages of 2024UChicago CMSC 23320 - The Best Commit Messages of 2024
UChicago CMSC 23320 - The Best Commit Messages of 2024
 
Basic Civil Engineering notes on Transportation Engineering & Modes of Transport
Basic Civil Engineering notes on Transportation Engineering & Modes of TransportBasic Civil Engineering notes on Transportation Engineering & Modes of Transport
Basic Civil Engineering notes on Transportation Engineering & Modes of Transport
 
FICTIONAL SALESMAN/SALESMAN SNSW 2024.pdf
FICTIONAL SALESMAN/SALESMAN SNSW 2024.pdfFICTIONAL SALESMAN/SALESMAN SNSW 2024.pdf
FICTIONAL SALESMAN/SALESMAN SNSW 2024.pdf
 

If 0.150 mL of 10^-7.5 dilution yielded an average of 256 coloniesp.pdf

  • 1. If 0.150 mL of 10^-7.5 dilution yielded an average of 256 colonies/plate, calculate the number of bacteria per mL of the original culture. Show all calculations. Solution The number of colony-forming units (CFU) = [no of cells]/ [volume of dilution x volume of diluted sample added to the plate] Now CFU per ml of dilution is 256 colonies divided by 0.150 ml plated = 1706.7 colony- forming units/ml of dilution. Dividing this number by the 10-7.5 dilution = 1706.7 / 10-7.5 = 1706.7 x 107.5 colony-forming units/ml of original culture sample