When does the centrosome duplicate? In parallel to chromosome segregation in mitosis In parallel with DNA replication in S-phase In G1 In G2 In an unregulated manner Solution When does the centrosome duplicate? Answer: In parallel with DNA replication in S-phase The centrosome appears to initiate cell division and Centrosome duplication is controlled by centriole replication.In centrosome duplication the mother and daughter centriole duplicates themselves.This process take place during the S-phase, at the same time as when the cell duplicates its DNA..