Describe how specific nucleosome positioning can promote transcriptional activation, or, alternatively, prevent it. If you wanted to precisely position a nucleosome on a given stretch of DNA, how could you do it? Describe how specific nucleosome positioning can promote transcriptional activation, or, alternatively, prevent it. If you wanted to precisely position a nucleosome on a given stretch of DNA, how could you do it? Solution Answer: Eukaryotes often possess complex euchromatin & heterochromatic organization with histone protein coiled around DNA so that a variety of enzymes may modify structure finally causes either gene expression by deacetylation or demethylation. Sometimes, high-uninterrupted gene expression may lead to due to methylation or epigenetic changes so that there are multiple level are there to exhibit gene regulation. DNA unwinding and template formation for coding mRNA from histone proteins; Eukaryotic cells possess increased levels of DNA binding proteins as transcriptional activators and gene repressors as well. Specific nucleosome positioning can promote transcriptional activation, or, alternatively, prevent it becuase the close proximity of DNA to either exit or entry points may have high likelihood to uncoil finally to undergo transcription from the DNA stretch using protein-nucleosomal remodeling complexes. The energy is used in the form of ATP to promote \"disruption of histone-DNA association\" finally promote gene expression. If you wanted to precisely position a nucleosome on a given stretch of DNA, it is crucial to ensue the accessibility of a binding site on \"histone-DNA binding point\" where it is the most critical point for \"activator or repressor\" to bind. We can change these positions on nucleosomes, using “DNA-binding proteins” to promote either transcription or repress transcription. Histone Acetylation only promotes gene expression but histone methylation inhibits gene expression During transcription regulation synergy can also achieved by binding one factor that change the structure of the chromatin thereby another binding site is going to be available more easily and readily or less readily. Initially, “transcription activating factor” is going to recruit a coactivator, which is followed by a histone acetyltransferase by acetylation associate with pulling chromatin apart. Regulatory domains of most activators interact with TFIID (transcription factor complexes) TFIIC, and TFIID to initiate transcription. Later repressor is going to recruit a corepressor, which is followed by a histone deacetylase that enable to squish the chromatin together. Synergy enable switching the genes on and off based on the DNA binding specificity finally promote integration of different transcription possible signals..