Cellulose is a long chain of linked sugar molecules that gives wood its remarkable strength. It is the main component of plant cell walls, and the basic building block for many textiles and for paper. Cotton is the purest natural form of cellulose. In the laboratory, ash less filter paper is a source of nearly pure cellulose.

1. Detection of Cellulose in
Milk
F a r h a n g H a j i H m a i d
I R A Q - K u r d i s t a n - s u l a y m a n i y a h
+ 9 6 4 7 7 0 1 4 2 0 9 6 2
F a r h a n g p d k . h a m i d @ g m a i l . c o m
5 / 2 0 / 2 0 1 6
Farhang Hamid
Cellulose isalongchainof linkedsugarmoleculesthatgiveswoodits
remarkable strength.Itisthe maincomponentof plantcell walls,and
the basic buildingblockformanytextiles andforpaper.Cottonisthe
purestnatural formof cellulose.Inthe laboratory, ashless filterpaperis
a source of nearlypure cellulose.

2. Detection of Cellulose in Milk
‫حليب‬ ‫في‬ ‫سليولوز‬ ‫تقدير‬
Cellulose in milk gives blue color with Iodine – Zinc Chloride reagent.
Cellulose is an organic compound with the formula (C6H10O5) n, a polysaccharide consisting of a linear chain of
several hundred to many thousands of β(1→4) linked D-glucose units Cellulose is an important structural
component of the primary cell wall of green plants, many forms of algae and the oomycetes. Some species of
bacteria secrete it to form biofilms. Cellulose is the most abundant organic polymer on Earth. The cellulose content
of cotton fiber is 90%, that of wood is 40–50% and that of dried hemp is approximately 57%.
Cellulose is mainly used to produce paperboard and paper. Smaller quantities are converted into a wide variety of
derivative products such as cellophane and rayon. Conversion of cellulose from energy crops into biofuels such as
cellulosic ethanol is under investigation as an alternative fuel source. Cellulose for industrial use is mainly obtained
from wood pulp and cotton.
Some animals, particularly ruminants and termites, can digest cellulose with the help of symbiotic micro-organisms
that live in their guts, such as Tri chonympha. In humans, cellulose acts as a hydrophilic bulking agent for feces and
is often referred to as a "dietary fiber".
Reagent: Iodine-Zinc Chloride reagent: Dissolve 20.0 gm ZnCl2 in 8.5 ml water and when cool,
introduce the iodine solution (3.0 gm potassium iodide and 1.5 gm iodine in 60 ml water) drop
by drop until iodine begins to precipitate.
Procedure:
-1 Take about 10.0 gm of milk in a 100 ml beaker.
-2 Add 50 ml of hot water and stirred thoroughly for about 2 minutes.
-3 Pour the mixture on a nylon cloth and wash the residue with 50 ml of hot water twice.
-4 Scrape the residue with a spatula and place it in a spotting plate.
-5 Stain a part of residue with Iodine-Zinc Chloride reagent and another part with iodine solution.
-6 Development of blue color in Iodine-Zinc Chloride reagent and absence of blue color in Iodine
Solution confirms presence of cellulose.
Note: The method is also applicable to milk products like curd, rabri and evaporated milk.