List and briefly define the types of cuts made by restrition enzymes. Solution Restriction enzymes are conventionally classified into four types on the beginning of 1) subunit conformation 2)cleavage position 3)sequence specificity and 4) Cofactor requirements. Type I enzymes are multisubunit, multiplex, incorporation restriction-and-moderation enzymes that cut DNA at random far from their identification sequences. Type II enzymes cut DNA at elucidate positions adjacent to or within their identification progression. Type III enzymes are also huge compound restriction-and-moderation enzymes. They cleave surface of their identification progression and necessitate two such progression in opposite alignment within the same DNA molecule to effectuate cleavage; they infrequently give absolute digests. Type IV enzymes accept improvement, representative methylated DNA and are illustrate by the McrBC and Mrr structure of E. coli..