SlideShare a Scribd company logo
1 of 1
Download to read offline
List and briefly define the types of cuts made by restrition enzymes.
Solution
Restriction enzymes are conventionally classified into four types on the beginning of
1) subunit conformation
2)cleavage position
3)sequence specificity and
4) Cofactor requirements.
Type I enzymes are multisubunit, multiplex, incorporation restriction-and-moderation enzymes
that cut DNA at random far from their identification sequences.
Type II enzymes cut DNA at elucidate positions adjacent to or within their identification
progression.
Type III enzymes are also huge compound restriction-and-moderation enzymes. They cleave
surface of their identification progression and necessitate two such progression in opposite
alignment within the same DNA molecule to effectuate cleavage; they infrequently give absolute
digests.
Type IV enzymes accept improvement, representative methylated DNA and are illustrate by the
McrBC and Mrr structure of E. coli.

More Related Content

Similar to List and briefly define the types of cuts made by restrition enzymes.pdf

Restriction enzymes d.sirohi
Restriction enzymes  d.sirohiRestriction enzymes  d.sirohi
Restriction enzymes d.sirohi
D. Sirohi
 

Similar to List and briefly define the types of cuts made by restrition enzymes.pdf (20)

ENZYMES USED IN GENETIC ENGINEERING
ENZYMES USED IN GENETIC ENGINEERINGENZYMES USED IN GENETIC ENGINEERING
ENZYMES USED IN GENETIC ENGINEERING
 
ppt.pptx
ppt.pptxppt.pptx
ppt.pptx
 
Restriction enzymes by devendra kumar
Restriction enzymes by devendra kumarRestriction enzymes by devendra kumar
Restriction enzymes by devendra kumar
 
restriction endonucleases.pptx
restriction endonucleases.pptxrestriction endonucleases.pptx
restriction endonucleases.pptx
 
Unit 2 Gene Cloning.pdf
Unit 2 Gene Cloning.pdfUnit 2 Gene Cloning.pdf
Unit 2 Gene Cloning.pdf
 
Fsqa 234.17
Fsqa 234.17Fsqa 234.17
Fsqa 234.17
 
genetic in stability and its role in cancer
genetic in stability and its role in cancer genetic in stability and its role in cancer
genetic in stability and its role in cancer
 
Restriction Modification Enzymes
Restriction Modification EnzymesRestriction Modification Enzymes
Restriction Modification Enzymes
 
restrictionendonucleases-170328152703.pdf
restrictionendonucleases-170328152703.pdfrestrictionendonucleases-170328152703.pdf
restrictionendonucleases-170328152703.pdf
 
Restriction endonucleases
Restriction endonucleasesRestriction endonucleases
Restriction endonucleases
 
Restriction enzymes
Restriction enzymesRestriction enzymes
Restriction enzymes
 
ENZYMES IN GENETIC ENGINEERING.ppt
ENZYMES IN GENETIC ENGINEERING.pptENZYMES IN GENETIC ENGINEERING.ppt
ENZYMES IN GENETIC ENGINEERING.ppt
 
Restriction enzymes treatment of DNA
Restriction enzymes treatment of DNARestriction enzymes treatment of DNA
Restriction enzymes treatment of DNA
 
Topoisomerases.PPTX nsjskskksnsbsbbsjsjl
Topoisomerases.PPTX nsjskskksnsbsbbsjsjlTopoisomerases.PPTX nsjskskksnsbsbbsjsjl
Topoisomerases.PPTX nsjskskksnsbsbbsjsjl
 
Restriction enzymes
Restriction enzymesRestriction enzymes
Restriction enzymes
 
Recombinant dna technology physics
Recombinant dna technology physicsRecombinant dna technology physics
Recombinant dna technology physics
 
Microbial genetics notes
Microbial genetics notesMicrobial genetics notes
Microbial genetics notes
 
Enzymes involved in rDNA technology.pptx
Enzymes involved in rDNA technology.pptxEnzymes involved in rDNA technology.pptx
Enzymes involved in rDNA technology.pptx
 
Types of restriction enzymes
Types of restriction enzymesTypes of restriction enzymes
Types of restriction enzymes
 
Restriction enzymes d.sirohi
Restriction enzymes  d.sirohiRestriction enzymes  d.sirohi
Restriction enzymes d.sirohi
 

More from eyezoneamritsar

How visual information is sent from the eye to the rest of the brain.pdf
How visual information is sent from the eye to the rest of the brain.pdfHow visual information is sent from the eye to the rest of the brain.pdf
How visual information is sent from the eye to the rest of the brain.pdf
eyezoneamritsar
 
Describe the major interactions among organisms in a food web.So.pdf
Describe the major interactions among organisms in a food web.So.pdfDescribe the major interactions among organisms in a food web.So.pdf
Describe the major interactions among organisms in a food web.So.pdf
eyezoneamritsar
 
Why are tourist destinations attempting to attract more visitors out.pdf
Why are tourist destinations attempting to attract more visitors out.pdfWhy are tourist destinations attempting to attract more visitors out.pdf
Why are tourist destinations attempting to attract more visitors out.pdf
eyezoneamritsar
 
High School Blues Evan and Alexia had been happily married for seven.pdf
High School Blues Evan and Alexia had been happily married for seven.pdfHigh School Blues Evan and Alexia had been happily married for seven.pdf
High School Blues Evan and Alexia had been happily married for seven.pdf
eyezoneamritsar
 
C# I need assitance on my code.. Im getting an error message Us.pdf
C# I need assitance on my code.. Im getting an error message Us.pdfC# I need assitance on my code.. Im getting an error message Us.pdf
C# I need assitance on my code.. Im getting an error message Us.pdf
eyezoneamritsar
 

More from eyezoneamritsar (20)

Identify 3 cell components that are shared in common between prokary.pdf
Identify 3 cell components that are shared in common between prokary.pdfIdentify 3 cell components that are shared in common between prokary.pdf
Identify 3 cell components that are shared in common between prokary.pdf
 
How many pairs of wires are in a standard Ethernet cableDefend or.pdf
How many pairs of wires are in a standard Ethernet cableDefend or.pdfHow many pairs of wires are in a standard Ethernet cableDefend or.pdf
How many pairs of wires are in a standard Ethernet cableDefend or.pdf
 
Fst can be used to measure the number of migrants per gegenetration .pdf
Fst can be used to measure the number of migrants per gegenetration .pdfFst can be used to measure the number of migrants per gegenetration .pdf
Fst can be used to measure the number of migrants per gegenetration .pdf
 
How visual information is sent from the eye to the rest of the brain.pdf
How visual information is sent from the eye to the rest of the brain.pdfHow visual information is sent from the eye to the rest of the brain.pdf
How visual information is sent from the eye to the rest of the brain.pdf
 
For coherently demodulated BPSK, what should EbN0 be in dB so as to.pdf
For coherently demodulated BPSK, what should EbN0 be in dB so as to.pdfFor coherently demodulated BPSK, what should EbN0 be in dB so as to.pdf
For coherently demodulated BPSK, what should EbN0 be in dB so as to.pdf
 
Cisco Systems The Supply Chain Story11. Study the networked suppl.pdf
Cisco Systems The Supply Chain Story11. Study the networked suppl.pdfCisco Systems The Supply Chain Story11. Study the networked suppl.pdf
Cisco Systems The Supply Chain Story11. Study the networked suppl.pdf
 
Describe four threats to high data availability and at least one mea.pdf
Describe four threats to high data availability and at least one mea.pdfDescribe four threats to high data availability and at least one mea.pdf
Describe four threats to high data availability and at least one mea.pdf
 
Describe the major interactions among organisms in a food web.So.pdf
Describe the major interactions among organisms in a food web.So.pdfDescribe the major interactions among organisms in a food web.So.pdf
Describe the major interactions among organisms in a food web.So.pdf
 
Consider the following circle of radius 5 centered at the origin. Fi.pdf
Consider the following circle of radius 5 centered at the origin.  Fi.pdfConsider the following circle of radius 5 centered at the origin.  Fi.pdf
Consider the following circle of radius 5 centered at the origin. Fi.pdf
 
Write one Conditional Signal Assignment VHDL code statement in the b.pdf
Write one Conditional Signal Assignment VHDL code statement in the b.pdfWrite one Conditional Signal Assignment VHDL code statement in the b.pdf
Write one Conditional Signal Assignment VHDL code statement in the b.pdf
 
Why are tourist destinations attempting to attract more visitors out.pdf
Why are tourist destinations attempting to attract more visitors out.pdfWhy are tourist destinations attempting to attract more visitors out.pdf
Why are tourist destinations attempting to attract more visitors out.pdf
 
Which pair of words are synonymsA ephemeral--evanescentB euphon.pdf
Which pair of words are synonymsA ephemeral--evanescentB euphon.pdfWhich pair of words are synonymsA ephemeral--evanescentB euphon.pdf
Which pair of words are synonymsA ephemeral--evanescentB euphon.pdf
 
Which of the following embryonic materials is matched with a correct.pdf
Which of the following embryonic materials is matched with a correct.pdfWhich of the following embryonic materials is matched with a correct.pdf
Which of the following embryonic materials is matched with a correct.pdf
 
We used 3 files with each lab project in Vivado. What do we call the.pdf
We used 3 files with each lab project in Vivado.  What do we call the.pdfWe used 3 files with each lab project in Vivado.  What do we call the.pdf
We used 3 files with each lab project in Vivado. What do we call the.pdf
 
To determine the distance between short and black on chromosome II th.pdf
To determine the distance between short and black on chromosome II th.pdfTo determine the distance between short and black on chromosome II th.pdf
To determine the distance between short and black on chromosome II th.pdf
 
High School Blues Evan and Alexia had been happily married for seven.pdf
High School Blues Evan and Alexia had been happily married for seven.pdfHigh School Blues Evan and Alexia had been happily married for seven.pdf
High School Blues Evan and Alexia had been happily married for seven.pdf
 
Suppose within your Web browser, you click on a link to obtain a Webp.pdf
Suppose within your Web browser, you click on a link to obtain a Webp.pdfSuppose within your Web browser, you click on a link to obtain a Webp.pdf
Suppose within your Web browser, you click on a link to obtain a Webp.pdf
 
Supports cukaryotic cellsSolutionEukaryotes are the organisms h.pdf
Supports cukaryotic cellsSolutionEukaryotes are the organisms h.pdfSupports cukaryotic cellsSolutionEukaryotes are the organisms h.pdf
Supports cukaryotic cellsSolutionEukaryotes are the organisms h.pdf
 
C# I need assitance on my code.. Im getting an error message Us.pdf
C# I need assitance on my code.. Im getting an error message Us.pdfC# I need assitance on my code.. Im getting an error message Us.pdf
C# I need assitance on my code.. Im getting an error message Us.pdf
 
Briefly define and describe what IASB and IFRS are.SolutionIAS.pdf
Briefly define and describe what IASB and IFRS are.SolutionIAS.pdfBriefly define and describe what IASB and IFRS are.SolutionIAS.pdf
Briefly define and describe what IASB and IFRS are.SolutionIAS.pdf
 

Recently uploaded

Salient Features of India constitution especially power and functions
Salient Features of India constitution especially power and functionsSalient Features of India constitution especially power and functions
Salient Features of India constitution especially power and functions
KarakKing
 

Recently uploaded (20)

This PowerPoint helps students to consider the concept of infinity.
This PowerPoint helps students to consider the concept of infinity.This PowerPoint helps students to consider the concept of infinity.
This PowerPoint helps students to consider the concept of infinity.
 
ComPTIA Overview | Comptia Security+ Book SY0-701
ComPTIA Overview | Comptia Security+ Book SY0-701ComPTIA Overview | Comptia Security+ Book SY0-701
ComPTIA Overview | Comptia Security+ Book SY0-701
 
FSB Advising Checklist - Orientation 2024
FSB Advising Checklist - Orientation 2024FSB Advising Checklist - Orientation 2024
FSB Advising Checklist - Orientation 2024
 
HMCS Max Bernays Pre-Deployment Brief (May 2024).pptx
HMCS Max Bernays Pre-Deployment Brief (May 2024).pptxHMCS Max Bernays Pre-Deployment Brief (May 2024).pptx
HMCS Max Bernays Pre-Deployment Brief (May 2024).pptx
 
On National Teacher Day, meet the 2024-25 Kenan Fellows
On National Teacher Day, meet the 2024-25 Kenan FellowsOn National Teacher Day, meet the 2024-25 Kenan Fellows
On National Teacher Day, meet the 2024-25 Kenan Fellows
 
Graduate Outcomes Presentation Slides - English
Graduate Outcomes Presentation Slides - EnglishGraduate Outcomes Presentation Slides - English
Graduate Outcomes Presentation Slides - English
 
SOC 101 Demonstration of Learning Presentation
SOC 101 Demonstration of Learning PresentationSOC 101 Demonstration of Learning Presentation
SOC 101 Demonstration of Learning Presentation
 
How to Create and Manage Wizard in Odoo 17
How to Create and Manage Wizard in Odoo 17How to Create and Manage Wizard in Odoo 17
How to Create and Manage Wizard in Odoo 17
 
Application orientated numerical on hev.ppt
Application orientated numerical on hev.pptApplication orientated numerical on hev.ppt
Application orientated numerical on hev.ppt
 
Google Gemini An AI Revolution in Education.pptx
Google Gemini An AI Revolution in Education.pptxGoogle Gemini An AI Revolution in Education.pptx
Google Gemini An AI Revolution in Education.pptx
 
Key note speaker Neum_Admir Softic_ENG.pdf
Key note speaker Neum_Admir Softic_ENG.pdfKey note speaker Neum_Admir Softic_ENG.pdf
Key note speaker Neum_Admir Softic_ENG.pdf
 
Explore beautiful and ugly buildings. Mathematics helps us create beautiful d...
Explore beautiful and ugly buildings. Mathematics helps us create beautiful d...Explore beautiful and ugly buildings. Mathematics helps us create beautiful d...
Explore beautiful and ugly buildings. Mathematics helps us create beautiful d...
 
Basic Civil Engineering first year Notes- Chapter 4 Building.pptx
Basic Civil Engineering first year Notes- Chapter 4 Building.pptxBasic Civil Engineering first year Notes- Chapter 4 Building.pptx
Basic Civil Engineering first year Notes- Chapter 4 Building.pptx
 
How to Manage Global Discount in Odoo 17 POS
How to Manage Global Discount in Odoo 17 POSHow to Manage Global Discount in Odoo 17 POS
How to Manage Global Discount in Odoo 17 POS
 
Unit-V; Pricing (Pharma Marketing Management).pptx
Unit-V; Pricing (Pharma Marketing Management).pptxUnit-V; Pricing (Pharma Marketing Management).pptx
Unit-V; Pricing (Pharma Marketing Management).pptx
 
Salient Features of India constitution especially power and functions
Salient Features of India constitution especially power and functionsSalient Features of India constitution especially power and functions
Salient Features of India constitution especially power and functions
 
Making communications land - Are they received and understood as intended? we...
Making communications land - Are they received and understood as intended? we...Making communications land - Are they received and understood as intended? we...
Making communications land - Are they received and understood as intended? we...
 
Kodo Millet PPT made by Ghanshyam bairwa college of Agriculture kumher bhara...
Kodo Millet  PPT made by Ghanshyam bairwa college of Agriculture kumher bhara...Kodo Millet  PPT made by Ghanshyam bairwa college of Agriculture kumher bhara...
Kodo Millet PPT made by Ghanshyam bairwa college of Agriculture kumher bhara...
 
ICT role in 21st century education and it's challenges.
ICT role in 21st century education and it's challenges.ICT role in 21st century education and it's challenges.
ICT role in 21st century education and it's challenges.
 
General Principles of Intellectual Property: Concepts of Intellectual Proper...
General Principles of Intellectual Property: Concepts of Intellectual  Proper...General Principles of Intellectual Property: Concepts of Intellectual  Proper...
General Principles of Intellectual Property: Concepts of Intellectual Proper...
 

List and briefly define the types of cuts made by restrition enzymes.pdf

  • 1. List and briefly define the types of cuts made by restrition enzymes. Solution Restriction enzymes are conventionally classified into four types on the beginning of 1) subunit conformation 2)cleavage position 3)sequence specificity and 4) Cofactor requirements. Type I enzymes are multisubunit, multiplex, incorporation restriction-and-moderation enzymes that cut DNA at random far from their identification sequences. Type II enzymes cut DNA at elucidate positions adjacent to or within their identification progression. Type III enzymes are also huge compound restriction-and-moderation enzymes. They cleave surface of their identification progression and necessitate two such progression in opposite alignment within the same DNA molecule to effectuate cleavage; they infrequently give absolute digests. Type IV enzymes accept improvement, representative methylated DNA and are illustrate by the McrBC and Mrr structure of E. coli.