EuFMD OS20

1. Figure 1. (A) Foot-and-mouth disease virus (FMDV) has seven distinct serotypes named O, A, C,
Asia1, and Southern African Territories 1 to 3. Each serotype is divided into several topotypes and
lineages by genetic characterization. (B) FMDV have been circulating in Southeast Asia such as
Vietnam, Laos, Myanmar, Thailand for a long time, and viral genetic substitutions would be
accumulated by selection pressure of transmission across hosts and immunity induced by infection
or vaccination. It is important to collect and analyze the same genotype virus that has already
occurred in Southeast Asia in order to cope with FMD in Korea in the near future.
ENHANCED DIAGNOSIS EFFICACY OF A NEWLY DEVELOPLED
ELISA KIT FOR FMDV IN POOL 1 REGION
BooMi La1, Jeong-won Lee1, Soyoon Ryoo1, Da-Rae Lim1, DongSook Lee1, HyunJi Lee1, Nguyen Dang Tho2, Thanh Long To2,
Bounlom Douangngeun3, Watthana Theppangna3, Seng Bunnary4, Sothyra Tum4, Jae-Myung Kim1, Sang-Ho Cha*,1
1 Animal and Plant Quarantine Agency, Ministry of Agriculture, Food and Rural affairs, Republic of Korea
2 National Center for Veterinary diagnostics, Department of Animal Health, Ministry of Agriculture and Rural Development, Vietnam
3 National Animal Health Laboratory, Department of Livestock and Fisheries, Ministry of Agriculture and Forestry, LAO PDR
4 National Animal Health and Production Research Institute,General Directorate of Animal Health and Production, Kingdom of Cambodia
References
Conclusion
The APQA ELISA kit had better sensitivity and specificity than Pirbright ELISA kit. Besides, the
APQA ELISA kit is quick and simple by saving approximately 2hrs, being compared with the
Pirbright ELISA kit. Conclusively, the APQA ELISA kit are expected to make a significant
contribution to control and prevention of FMD in POOL 1 region.
ResultsIntroduction
Foot-and-Mouth disease (FMD) is a highly contagious viral disease in the cloven-hoofed animals
such as pig and cattle. FMDV has been highly prevalent in most of Southeast Asia countries
including Vietnam, Laos, Myanmar and Cambodia of POOL 1 region, which Republic of Korea
belongs to. As FMD outbreaks caused by FMDV virus serotype O and A occurred throughout
POOL1 region, it is important to identify serotypes from the outbreaks for better efficiency in
further characterization of FMDV and prompt control measures in action. In this study, FMDVs
were isolated from clinical samples of FMD outbreaks in 2015-2019 of Vietnam (n=9), Laos (n=12),
Myanmar (n=1), and Cambodia (n=30) through the international collaborative research projects
and subjected to performance comparison between OIE reference ELISA kit (Pirbright kit) and
APQA ELISA kit newly developed from our laboratory.
Materials and Methods
Vesicle, epithelium or saliva samples
:Myanmar (n=1), Laos (n=12), Vietnam (n =9), Cambodia (n=30)
Molecular Devices SpectraMax M2 Microplate Reader w/ Softmax
Table1. FMDV isolated from Vietnam, Laos, Cambodia, Myanmar in 2015-2019
Figure 2. Results of APQA and Pirbright Ag ELISA
In most O type FMDV samples, APQAAg ELISA showed better sensitivity than Pirbright Ag
ELISA. In the case of A type FMDV sample, APQA was better in Laos(F), but Pirbright, which
has two Mab strips, showed higher sensitivity in the second Mab strip in Cambodia samples(B).
Meanwhile, non-specific A strip(B) in Pirbright ELISA were observed positive for O type samples.
A
B
C
D
E
F
G
H
This research was supported by the Research of Animal and Plant Quarantine Agency, Ministry of
Agriculture, Food and Rural Affairs (MAFRA), Republic of Korea.
References:
VP. Le et al., 2016. BMC Vet Res 12:269
VP. Le et al., 2010. Vet. Microbiol. 45:220-229
A B
Pirbright Ag ELISA APQAAg ELISA
Myanmar 2016 MYA16 cattle O/ME-SA/Ind-2001d
19CAM2 cattle
19CAM3 cattle
19CAM4 cattle
19CAM5 cattle
19CAM7 cattle
19CAM8 cattle
19CAM9 cattle
19CAM11 cattle
19CAM12 cattle
19CAM13 cattle
19CAM16 cattle A/ASIA/SEA97
19CAM19 cattle A/ASIA/SEA97
19CAM20 cattle A/ASIA/SEA97
19CAM22 cattle
19CAM24 cattle
19CAM25 cattle
19CAM26 cattle
19CAM27 cattle
19CAM28 cattle
19CAM29 cattle
19CAM30 cattle A/ASIA/SEA97
19CAM31 cattle
19CAM32 cattle
19CAM33 cattle
19CAM34 cattle
19CAM35 cattle
19CAM36 cattle
19CAM37 cattle
19CAM41 cattle
19CAM46 cattle A/ASIA/SEA97
O/ME-SA/PanAsia
O/ME-SA/PanAsia
Cambodia
2018-2019
O/ME-SA/PanAsia
Location SAMPLE SPECIES Genotype
19VN2 cattle
19VN8 buffalo
19VN12 pig
19VN16 pig
19VN20 pig
19VN21 pig
19VN31 pig
19VN34 pig
19VN35 pig
LA1 buffalo
LA3 Cow
LA4 Cow
LA5 Cow
LA9 Cow O/SEA/Mya-98
LA11 Cow O/ME-SA/Ind-2001d
LA17 Cow
LA19 Cow
LA20 Cow
LA21 Cow
LA22 Cow A/ASIA/Sea97
19LA5 Cow O/ME-SA/PanAsia
Vietnam
2018-2019
O/ME-SA/PanAsia
Laos
2015-2018
O/ME-SA/Ind-2001d
O/ME-SA/PanAsia
Location SAMPLE SPECIES Genotype
O/SEA/Mya-98