Skin is the most alive organ in our body. It plays many significant roles such as protection of inner organs from external stresses and damages including UV irradiation, bacterial, fungal and viral infections, direct contacts, etc. Appropriate hydration of outer layer of skin is very important item to maintain integrity and barrier functions of a healthy skin. If skin becomes dehydrate it is prone to many damages such as roughness, wrinkles, easy erosions, infections, itching and lack of shine and natural beauty. Alpha-glucan oligosaccharide is a glucose oligomer exhibiting a degree of polymerization ranging from 2 to 10. It is use in cosmetic product as an emollient and conditioning agent. It is a saccharine (sugar molecule) that has water-binding abilities. The objective of this study was to evaluate the in vivo moisturizing efficacy of alpha-glucan oligosaccharide components used in syndet bar in healthy female subjects.

1. EVALUATION OF THE IN VIVO MOISTURIZING
EFFICACY OF ALPHA GLUCAN
OLIGOSACCHARIDE USED IN SYNDET BAR
Mohammad Baghaei PharmD1, Mohammad Reza Nateghi MD1,
Hamid Reza Zolfaghari MD1, Amir Houshang Ehsani MD2, Behrooz Barikbin MD3
1. Department of R&D, Facedoux Dermocosmetic Co., Tehran, Iran
2. Department of Dermatology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3. Assistant Professor of Dermatology, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2. Cleansing …
 The art of cleansing has progressed immensely
over several thousand years…
 Cleansers in dermatology and cosmetic
The Greek physician Galen (130-200 AD) and the Iranian eighth
century chemist Gabiribne Hayyan were the first to have written
about the use of soap as a body-cleansing agent.
Gabiribne Hayyan

3. Cleanser Composition
Table 1: The constituents of skin cleansers
The type and amount of surfactant in a
cleansing agent has a bearing on its drying and
irritancy potential.

4. Evolution of modern day cleansers
Cleansers can be divided into three basic types:
 Soaps
 Syndet bars
 Combars

5. Dermatologic Syndet bars
(synthetic detergent)
• Dermatologic Syndet (synthetic detergent) bars
have a non-soap synthetic surfactant such as
fatty acid isothionates, sulfosuccinic acid
esters as their principal ingredient.
Syndets have a neutral or slightly acidic pH
and are less irritating to skin and do not form
soap scum layer.

6. Cleansing and skin barrier function
• Day-to-day challenges, such as changes in
humidity, temperature, and daily cleansing, can affect skin’s
barrier properties and its ability to hold water.
Hyper-hydration occurring during regular
wash and after extended water exposure is
Because surfactant binding
followed by shrinking as water
reduces the skin’s ability to
evaporates, leading to drying stresses.
retain water, skin often returns
Swelling facilitates the penetration of
to a state of lower hydration
surfactants into deeper layers, possibly
after washing.
leading to a biochemical response such as
irritation and itch.
Surfactant binding to SC proteins can cause
significant protein denaturation, leading to
barrier damage.

7. The Ecoflora – a microbiological defense barrier
• Mary Marples (The Ecology of the human skin, 1965)

8. Saprophytic flora and pathogenic flora
• The barrier between saprophytic flora and
pathogenic flora is often difficult to define.

9. Probiotic, Prebiotic
Probiotic : Refers to living microorganisms, generally bacteria, used in
foodstuffs in order to enhance the quality of the
intestinal flora. In man, bifidus probiotics aid
digestion and are said to improve the immune
defense system.
Prebiotic : Non-digestible food ingredient that
has a beneficial effect on health by selectively
stimulating the growth and/or metabolic
activity of one or a limited number of intestinal
& Skin bacteria.

10. BIOECOLIA
(Alpha-glucan oligosaccharide)
Its bioselectivity as a substrate will enable it to
restrict the growth of pathogenic flora.
It is use in cosmetic product as an emollient
and conditioning agent
It is a saccharine (sugar molecule) that has
water-binding abilities.

12. Our study …
• The objective of our study was to evaluate the
in vivo moisturizing efficacy of alpha-glucan
oligosaccharide components used in syndet
bar in healthy female subjects.

13. Methods & Materials
128 healthy female subjects, between 18-65
years of age, having a dry skin, were randomly
selected into case and treated groups for this
clinical trial study.
Two types of syndet bar composed of Tensianol
815 (as control) and Tensianol plus alphaglucan oligosaccharide (as experiment) were
used.
The syndet bars were applied twice a day as a
hand washing by the case and treated
groups, over a sixty-day period. The
moisturizing effect was evaluated by
corneometry at T0, T+2 days, T+5 days and
T+60 days.
Data was analyzed by paired and two-tailed t-test.

14. Results
Mean age of participants was 36.8±10.5
The mean level of skin hydration measured by
corneometry was 35.8±5.7 in treated group
and 35.9±5.5 in control group at beginning of
the study (T0).
The mean level of skin hydration at T+2 and T+5 days after
application of syndet bar was 36.1±7.0 and 37.3±10.1 in treated
skin and 35.4±6.7 and 35.9±5.1 in control group respectively.
In the treated group, there was no significant difference between baseline measurement (T0)
and T+2 and T+5 days measurement (P<0.05). Also there was no significant difference between
baseline measurement and T+2 and T+6 days measurement in the control group (P>0.05).
Besides, there was no significant difference between treated skin and control skin at T+2 and
T+5 days measurement (P<0.05).

15. Results…
Long-term application of syndet bar showed that the mean
level of skin hydration in the treated skin at T+60 days was
40.3 8.9 and in the control skin was 35.2 6.4.
There was significant difference between baseline measurements (T0) and T+60
days in treated groups (P<0.05) but no significant different was seen in control
group at T+60 days.
A significant difference was seen
between treated group and control
group at T+60 days of measurement
(P<0.05).

17. Conclusion
The study showed that application of a syndet bar composed of
alpha-glucan oligosaccharide component was not able to improve
moisturizing of skin in short-term usage but it is able to improve
moisturizing long-term usage (approximately 14.5%). No
unwanted or adverse event has been reported by the subjects
during the study time course.
It is able to improve moisturizing long-term usage
(approximately 14.5%)

18. It is my pleasure introduce you a new application in
dermatological syndet bar field :
It is prebiotic syndet bar

23. Do widzenia