This study aimed to prepare and evaluate aloe gel beads for use in dermal formulations. Aloe gel beads were created by extruding an aloe gel-sodium alginate mixture through a syringe into a calcium chloride solution, forming beads. The beads and a conventional aloe gel were used to prepare novel and conventional lotions. The lotions were evaluated for characteristics like morphology, pH, rheology, stability, and skin sensitivity. Results indicated the novel lotion containing aloe gel beads had better stability and rheological properties compared to the conventional lotion. Therefore, the formulation containing aloe gel beads could provide more effective therapeutic benefits than normal lotions.

1. Journal of Global Biosciences, Vol. 2(6), 2013, pp. 206-216
*AMADEEPSINGH BAL, TASNEEM ARA, ABDUL SAMIEH DEVA and SANJAY SHARMA
*Sri Sai College of Pharmacy Badhani Pathankot Punjab
Presented by
P. ANUJA
M.PHARM (THIRD SEM)
C.L.BAID METHA COLLEGE OF
PHARMACY
1

2. CONTENT
 ABSTRACT
 INTRODUCTION
 CHEMICAL COMPOSITION
 POTENTIAL CLINICAL BENEFITS OF ALOE
VERA
 MATERIALS AND METHOD
 EVALUATION PARAMETERS
 RESULT AND DISSCUSION
 CONCLUSION
 REFERENCES 2

3. ABSTRACT
 This work has been regulated regarding preparation and
evaluation of Aloe Gel Beads.
 the morphological and rheological properties of the aloe
gel beads was observed.
 An attempt was made to prepare and evaluate the dermal
formulation of Aloe Gel Beads.
 Such dermal preparation may provide potential beneficial
effects as compared to conventional available dermal
preparation with less or no side
effect.
3

4. INTRODUCTION
 Aloe vera is a semi-tropical ,ever green perennial plant. Aloe
vera can be found in a range of health care and cosmetic
products such as hand lotions, shampoos, cosmetics, beverages
and dietary supplements.
 Aloe vera gel is used for its emollient and wound healing
activities.
 Several biological activities have been reported for aloe
polysaccharides, including antiviral, antibacterial, anti-tumor,
wound healing properties.
 Aloe vera gel having the property to enhance the permeation of
several drug molecule.so it is used as a permeation enhancer in
several formulation.
4

5. CHEMICAL COMPOSITION
Aloe gel
99% water Other ingredients
5

6. CHEMICAL COMPOUND BENEFITS
polysaccharide emollient
glucomannan moisturizer
Acemannan accelerates wound healing
bradykinase, cellulase, carboxypeptidase, catalase,
amylase and an oxidase. Magnesium lactate
prevent itching
salicylic acid and other antiprostaglandin relieve inflammation
vitamins A, C, E, B1(thiamine),B3(niacin),
B2 (riboflavin), choline and folic acid.,
antioxidant
saponine(3%) cleansing and antiseptic
properties
anthraquinone glycosides (aloin, aloe-emodin and
barbaloin)
laxatives
6

7. PHYSICAL PROPERTIES OF ALOE GEL
Colour clear yellow/green
liquid
PH 3.5-4.7
Refractive index 1.3340-1.3355
Specific gravity 1.0030-1.0070
7

8. POTENTIAL CLINICAL BENEFITS
OF ALOE GEL
8

9. Burn treatment Antibacterial
Anti-inflammatorywound Healing
Anti diabetes Other Activities
AntifungalAntiviral
Gastrointestinal
function and ulcers
Antineoplastic
9

10. Wound Healing
Clinical studies have shown that the gel preparations accelerate wound healing. In vivo
studies have demonstrated that the gel promotes wound healing by directly stimulating
the activity of macrophages and fibroblasts
Anti-inflammatory
Fresh gel significantly reduced acute inflammation in rats (carrageenin-induced paw
oedema), The gel appears to exert its anti inflammatory
activity through bradykinase activity and thromboxane B2 and prostaglandin F2
inhibition.
Burn treatment
Aloe vera gel has been used for the treatment of radiation burns. the fresh gel was more
effective than the cream. Complete healing was observed, after treatment with fresh gel,
for radiation burns.
Antibacterial
Aloe vera gel is bacteriostatic or bactericidal against a variety of common wound-
infecting bacteria like Staphylococcus aureus,, E.coli,. Aloe-emodin
also inhibits the growth of Helicobacter pylori in a dose-dependent fashion.
10

11. Antiviral
Aloe vera can be used in the treatment of HIV-AIDS. This is attributed to the anti-viral and
immuno-modulating properties of acemannan.
Antifungal
Growth of a yeast candidia albicans is somewhat inhibited by a processed Aloe vera gel
preparation.
Antineoplastic
Lecitin from the leaves of Aloe vera have haemoagglutinating properties and fresh
preparations also promoted growth of normal human cells in culture but inhibited tumor
cell growth.
Gastrointestinal function and ulcers
Aloe vera gel is being used for internal inflammatory conditions. aloctin inhibited the
volume of gastric juice, acid and pepsin in rats. And cure ulcer induced by indomethacin.
Aloe gel may have a therapeutic effect in inflammatory bowel Disease in human.
Anti diabetes
Evidence for anti-glycation properties of Aloe vera can be found in studies with diabetics,
both animal and human Aloe vera assists in reducing blood glucose to more normal levels
specifically in type I and type II diabetics, not in non-diabetics
11

12. 12

13. Wash a healthy aloe vera leaf under
running water.
Using a knife, cut the serrated edges
off the leaf
Make a lengthwise incision in the
middle of the leaf to peel off the skin.
Place knife under the rind & slide it
along the length of the leaf to remove
the skin.
Repeat the same for the other half of
the skin.
 Scoop out the pulp & blend it for
30sec
Transfer the gel into an airtight
container.
13

14. Clean the leaf with water
chop off the serrated ends
with a knife.
Peel off the upper skin of
the leaf.
 Place the leaf over a bowl
&using multiple short strokes,
scrape out the gel.
Transfer the collected gel
into an air tight container
 store it in refrigerator.
14

15. GEL STABILISATION
Upon cutting , When gel exposed to air, it rapidly oxidizes,
decomposes and looses much of its biological activities.
Various methods are available for gel stabilization. Like
 heat treatment,
cold processing
15

16. Heat treatment
 In the heat treatment processing, sterilization is achieved by
subjecting the aloe liquid to heat.
 A. Vera gel essentially remains intact when the gel is heated at
65◦C for periods less than 15 minutes. After heat treatment, the
juice is flash cooled to 5 °C or below within 15 sec to preserve
biological activity.
 Extended heating of Aloe vera gel denatures the amino acids,
deactivates the enzymes and breaks the lengths of the
polysaccharide chains
16

17. cold processing
 cold processing is the superior method to ensure and
protect the activity and integrity of Aloe vera’s
constituents.
 In this method there is no application of heat and enzymes
(glucose oxidase and catalase )are used to inhibit growth
of aerobic organisms within the gel.
 Other sterilization steps reported in the cold processing
include exposing the gel to ultraviolet light followed by
micron filtration
17

18. ALOE VERA IN COSMETICS
Aloe vera ha been added in many of the cosmetics, because
of its rejuvenating action.
This action is achieved by following steps.
Hydration of skin by polysaccharide (moisturizer)
Absorption of aloe into the skin
Stimulate fibroblast replication in faster rate.
Fibroblast produce collagen and elastin fiber.
Because of this the skin becomes more elastic and less
wrinkled.
18

19.  Aloe produce smoother skin by sticking superficial
flaking epidermal cell
 It prevent liver spot(ageing skin) by interfering
the enzyme that produces melanin
19

20. PREPRATION OF GEL BEADS
300mg (SA) 10ml(dt) + 2g (A.gel)
dissolved
15min
(1000rpm)
mixture
Extruded via
syringe(no 18)
5% cacl2 sol
Aloe gel beads in
solution
filtration
0.45mm
gentle agitation
(37º)
(5min)
Aloe gel bead
20

21. Prepration of lotion containing
beads and aloe gel
 Novel lotion:
suspending 2gm of bead in 15ml calamine lotion.
 Conventional lotion:
incorporating 2gm of aloe gel in 15ml of calamine
lotion
21

22. Prepared aloe gel beads
22

23. EVALUATION OF GEL BEADS
 Morphology of gel beads
 Crushing property
 pH
 Rheological studies
 Stability study
 Sensitivity of lotion:
23

24. Morphology of gel beads
The mean diameter of dried beads were observed using
optical microscopy.
24

25. Crushing property
50gm beads of aloe gel were pressed over butter paper to
observe the crushing of
beads to access its handling during application of lotion.
25

26. pH
PH of 15ml of lotion was determined by using pH meter
and colour is observed with naked eye to observe the
compatibility with skin.
26

27. Rheological studies
 Both lotion were allowed to stand for 24hrs and
sedimentation ratio was calculated by using standard
formula .
 redispersability of lotion(15 ml) observed after 24hrs
by its flocculation .
 Viscosity of both lotions was measured by Brookfield
viscometer.
 Cracking property for 5ml of each lotion was
determined by keeping them at refrigerated
temperature ( -4 degree centigrade) for 24hrs and
separation of solid and liquid phase were observed.
27

28. Stability study
Stability study of both lotions is carried out in terms of
physical evaluation by naked eye at room temperature.
28

29. Sensitivity of lotion
Six volunteers (25-30 yrs), after applying the both lotions on
forearm for 20 min, observed for any irritation or edema
formation
29

30. RESULT AND DISSCUSION
30

31. CONCLUSION
 Both novel and conventional lotion were evaluated for
physical and rheological studies
 Based on the result of the studies, novel lotion containing
aloe gel beads shows better stability and rheological
property.
 So this formulation gives better therapeutic activity as
compared to normal conventional lotion.
 Overall study revealed that as compared to conventional
lotion, the prepared novel lotion is more stable and
effective
31

32. REFERENCE
 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC35511
17/
 https://www.researchgate.net/publication/288718153_
Novel_preparation_and_evaluation_of_lotion_contain
ing_aloe_gel_beads
 https://www.fabhow.com/how-to-make-pure-aloe-
vera-gel.html
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