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Fungi
Characteristics of fungi
 Diverse group of chemo heterotrophs
 Over 100,000 fungal species identified
 Only about 100 are human or animal
pathogens
 Saprophytes – Digest dead organic matter
 Parasites –Obtain nutrients from tissues of
organisms
 Most are aerobes or facultative anaerobes
The Characteristics of Fungi
• Heterotrophy - 'other food'
* Saprophytes or saprobes - feed on dead
tissues or organic waste (decomposers)
* Symbionts - mutually beneficial
relationship between a fungus and another
organism
* Parasites - feeding on living tissue of a
host.
• Parasites that cause disease are called
pathogens.
Morphology of fungi
• Fungi are eukaryotic protista; differ from bacteria and
other prokaryotes.
1. Cell walls containing chitin (rigidity & support),
mannan & other polysaccharides
2. Cytoplasmic membrane contains ergosterols
3. Possess true nuclei with nuclear membrane & paired
chromosomes
4. Cytoplasmic contents include mitochondria and
endoplasmic reticulum
5. Divide asexually, sexually or by both
6. Unicellular or multicellular
7. Most fungi are obligate or facultative aerobes
Morphology of fungi
• Body form
* unicellular
* filamentous (tube-like
strands called hypha
(singular) or hyphae
(plural)
* mycelium = aggregate
of hyphae
* sclerotium = hardened
mass of mycelium that
generally serves as an
overwintering stage.
* multicellular, such as
mycelial cords,
rhizomorphs, and fruit
bodies (mushrooms)
mycelium
fruiting bodies
both are
composed
of hyphae
Hyphae
• Elongation of apical cell
produces a tubular,
thread like structure
called hypha.
• Hyphae may be septate
or nonseptate.
• Hard wall of chitin
• Crosswalls may form
compartments (± cells)
• Multinucleate
• Grow at tips
Hyphal growth
• Hyphae grow from their tips
• Mycelium = extensive, feeding web of hyphae
• Mycelia are the ecologically active bodies of
fungi
This wall is rigid Only the tip wall is plastic and stretches
Modifications of hyphae
Mycelium
• Mass of branching intertwined hyphae
a. Vegetative Mycelium- hyphae that
penetrate the supporting medium and absorb
nutrients
b. Aerial Mycelium- hyphae projects above
the surface of medium and bear the
reproductive structure called conidia.
Classification of fungi
1.Morphological classification
2.Systematic classification
Morphological classification
1. Yeasts
2. Yeast-like fungi
3. Filamentous fungi (molds)
4. Dimorphic fungi
Yeasts
• These occur in the form of round or oval
bodies which reproduce by an asexual
process called budding in which the cell
develops a protuberance which enlarges and
eventually separates from the parent cell.
• Yeasts colonies resemble bacterial colonies
in appearance and in consistency
• Examples are- Saccharomyces cerevisiae,
Cryptococcus neoformans
Yeast-Like
• Yeast like fungi grow partly as yeast and
partly as elongated cells resembling hyphae.
The latter form a pseudomycelium.
Example: Candida albicans
Molds or Filamentous Fungi
• The basic morphological elements of
filamentous fungi are long branching filaments or
hyphae, which intertwine to produce a mass of
filaments or mycelium
• Colonies are strongly adherent to the medium and
unlike most bacterial colonies cannot be emulsified
in water
• The surface of these colonies may be velvety,
powdery, or may show a cottony aerial mycelium.
• Reproduce by the formation of different types of
spores
• Example: Dermatophytes, Aspergillus, Penicillium,
Mucor, Rhizopus
Dimorphic Fungi
• These are fungi which exhibit a yeast form
in the host tissue and in vitro at 370C on
enriched media and mycelial form in vitro
at 250C
• Examples:
• Histoplasma capsulatum
• Blastomyces dermatitidis
• Coccidioides immitis
• Paracoccidoides brasiliesis
• Penicillium marneffei
• Sporothrix schenckii
Histoplasma capsulatum -
Dimorphism
• Filamentous mold in environment
-Thin septate hyphae, microconidia, and
tuberculate macroconidia (8-14 µm)
• Budding yeast (2-4 µm) in tissue
-Dimorphic transition is thermally
dependent and reversible (25°C 37°C).
Systematic classification
• Based on sexual spores formation:
4 classes
1. Zygomycetes
2. Ascomycetes reproduce sexually
3. Basidiomycetes
4. Deuteromycetes (fungi imperfectii)
Zygomycetes
• Lower fungi
• Broad, nonseptate hyphae
• Asexual spores
Sporangiospores: present
within a swollen sac- like
structure called Sporangium
Examples: Rhizopus, Absidia,
Mucor
Ascomycetes
• Sexual spores called
ascospores are
present within a sac
like structure called
Ascus.
• Each ascus has 4 to
8 ascospores
• Includes both yeasts
and filamentous fungi
Ascomycetes
• Narrow, septate hyphae
• Asexual spores are called conidia
borne on conidiophore
• Examples: Penicillium, Aspergillus
Basidiomycetes
• Sexual fusion results in the formation of
a club shaped organ called base or
basidium which bearspores called
basidiospores
• Examples: Cryptococcus neoformans,
mushrooms
Deuteromycetes or Fungi
imperfectii
• Group of fungi whose sexual phases are
not identified
• Grow as molds as well as yeasts
• Most fungi of medical importance
belong to this class
• Examples: Coccidioides immitis,
Paracoccidioides brasiliensis, Candida
albicans
Reproduction and sporulation
• Types of fungal spores
1.Sexual spores
2.Asexual spores
Sexualspores
• Sexual spore is formed by fusion of
cells and meiosis as in all forms of
higher life
• Ascospores
– Ascus
– Ascocarp
• Basidiospores
• Zygospores
Asexual spores
These spores are produced by mitosis
1.Vegetative spores
2.Aerial spores
Vegetative spores
• Blastospores: These
are formed by budding
from parent cell, as in
yeasts
• Arthrospores –
formed by
segmentation &
condensation of
hyphae
• Chlamydospores –
thick walled resting
spores developed by
rounding up and
Aerial spores
1. Conidiospores
Spores borne externally on
sides or tips of hyphae are
called conidiospores or
simply conidia
2. Microconidia- conidia are
small and single
3. Macroconidia- conidia are
large
4. Sporangiospores- spores
forms Within the
sporangiophores.
• Microconidia - Small,
single celled
• Macroconidia – Large
and septate and are
often multicellular
Pictures of fungi on LPCBmount
Aspergillus Penicillium
THANK YOU

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FUNGI FULL NEW.pdf

  • 2. Characteristics of fungi  Diverse group of chemo heterotrophs  Over 100,000 fungal species identified  Only about 100 are human or animal pathogens  Saprophytes – Digest dead organic matter  Parasites –Obtain nutrients from tissues of organisms  Most are aerobes or facultative anaerobes
  • 3. The Characteristics of Fungi • Heterotrophy - 'other food' * Saprophytes or saprobes - feed on dead tissues or organic waste (decomposers) * Symbionts - mutually beneficial relationship between a fungus and another organism * Parasites - feeding on living tissue of a host. • Parasites that cause disease are called pathogens.
  • 4. Morphology of fungi • Fungi are eukaryotic protista; differ from bacteria and other prokaryotes. 1. Cell walls containing chitin (rigidity & support), mannan & other polysaccharides 2. Cytoplasmic membrane contains ergosterols 3. Possess true nuclei with nuclear membrane & paired chromosomes 4. Cytoplasmic contents include mitochondria and endoplasmic reticulum 5. Divide asexually, sexually or by both 6. Unicellular or multicellular 7. Most fungi are obligate or facultative aerobes
  • 5.
  • 6. Morphology of fungi • Body form * unicellular * filamentous (tube-like strands called hypha (singular) or hyphae (plural) * mycelium = aggregate of hyphae * sclerotium = hardened mass of mycelium that generally serves as an overwintering stage. * multicellular, such as mycelial cords, rhizomorphs, and fruit bodies (mushrooms)
  • 8. Hyphae • Elongation of apical cell produces a tubular, thread like structure called hypha. • Hyphae may be septate or nonseptate. • Hard wall of chitin • Crosswalls may form compartments (± cells) • Multinucleate • Grow at tips
  • 9. Hyphal growth • Hyphae grow from their tips • Mycelium = extensive, feeding web of hyphae • Mycelia are the ecologically active bodies of fungi This wall is rigid Only the tip wall is plastic and stretches
  • 11. Mycelium • Mass of branching intertwined hyphae a. Vegetative Mycelium- hyphae that penetrate the supporting medium and absorb nutrients b. Aerial Mycelium- hyphae projects above the surface of medium and bear the reproductive structure called conidia.
  • 12. Classification of fungi 1.Morphological classification 2.Systematic classification
  • 13. Morphological classification 1. Yeasts 2. Yeast-like fungi 3. Filamentous fungi (molds) 4. Dimorphic fungi
  • 14. Yeasts • These occur in the form of round or oval bodies which reproduce by an asexual process called budding in which the cell develops a protuberance which enlarges and eventually separates from the parent cell. • Yeasts colonies resemble bacterial colonies in appearance and in consistency • Examples are- Saccharomyces cerevisiae, Cryptococcus neoformans
  • 15.
  • 16.
  • 17. Yeast-Like • Yeast like fungi grow partly as yeast and partly as elongated cells resembling hyphae. The latter form a pseudomycelium. Example: Candida albicans
  • 18. Molds or Filamentous Fungi • The basic morphological elements of filamentous fungi are long branching filaments or hyphae, which intertwine to produce a mass of filaments or mycelium • Colonies are strongly adherent to the medium and unlike most bacterial colonies cannot be emulsified in water • The surface of these colonies may be velvety, powdery, or may show a cottony aerial mycelium. • Reproduce by the formation of different types of spores • Example: Dermatophytes, Aspergillus, Penicillium, Mucor, Rhizopus
  • 19.
  • 20. Dimorphic Fungi • These are fungi which exhibit a yeast form in the host tissue and in vitro at 370C on enriched media and mycelial form in vitro at 250C • Examples: • Histoplasma capsulatum • Blastomyces dermatitidis • Coccidioides immitis • Paracoccidoides brasiliesis • Penicillium marneffei • Sporothrix schenckii
  • 21. Histoplasma capsulatum - Dimorphism • Filamentous mold in environment -Thin septate hyphae, microconidia, and tuberculate macroconidia (8-14 µm) • Budding yeast (2-4 µm) in tissue -Dimorphic transition is thermally dependent and reversible (25°C 37°C).
  • 22. Systematic classification • Based on sexual spores formation: 4 classes 1. Zygomycetes 2. Ascomycetes reproduce sexually 3. Basidiomycetes 4. Deuteromycetes (fungi imperfectii)
  • 23. Zygomycetes • Lower fungi • Broad, nonseptate hyphae • Asexual spores Sporangiospores: present within a swollen sac- like structure called Sporangium Examples: Rhizopus, Absidia, Mucor
  • 24. Ascomycetes • Sexual spores called ascospores are present within a sac like structure called Ascus. • Each ascus has 4 to 8 ascospores • Includes both yeasts and filamentous fungi
  • 25. Ascomycetes • Narrow, septate hyphae • Asexual spores are called conidia borne on conidiophore • Examples: Penicillium, Aspergillus
  • 26. Basidiomycetes • Sexual fusion results in the formation of a club shaped organ called base or basidium which bearspores called basidiospores • Examples: Cryptococcus neoformans, mushrooms
  • 27. Deuteromycetes or Fungi imperfectii • Group of fungi whose sexual phases are not identified • Grow as molds as well as yeasts • Most fungi of medical importance belong to this class • Examples: Coccidioides immitis, Paracoccidioides brasiliensis, Candida albicans
  • 28. Reproduction and sporulation • Types of fungal spores 1.Sexual spores 2.Asexual spores
  • 29. Sexualspores • Sexual spore is formed by fusion of cells and meiosis as in all forms of higher life • Ascospores – Ascus – Ascocarp • Basidiospores • Zygospores
  • 30. Asexual spores These spores are produced by mitosis 1.Vegetative spores 2.Aerial spores
  • 31. Vegetative spores • Blastospores: These are formed by budding from parent cell, as in yeasts • Arthrospores – formed by segmentation & condensation of hyphae • Chlamydospores – thick walled resting spores developed by rounding up and
  • 32. Aerial spores 1. Conidiospores Spores borne externally on sides or tips of hyphae are called conidiospores or simply conidia 2. Microconidia- conidia are small and single 3. Macroconidia- conidia are large 4. Sporangiospores- spores forms Within the sporangiophores.
  • 33. • Microconidia - Small, single celled • Macroconidia – Large and septate and are often multicellular
  • 34. Pictures of fungi on LPCBmount Aspergillus Penicillium
  • 35.