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MARCO
LOBREGATMarketing & Communication
Strategist
Food & Tourism Advocate
Hypersocial Entrepreneur
facebook.com/marc
o.lobregat
@ministryshrooms @mlobregat
Comprehensive Training
forOysterMushroomGrowing
Objective
• Knowledge about Mushrooms and Health
Benefits.
• Know the step by step Mushroom Culture
and Propagation, Bagging, Harvesting,
Processing and Marketing
• Appreciate mushrooms as a sustainable
livelihood and alternative to meat.
• Apply the technology as you will be
encouraged to grow mushrooms.
Mushrooms
Mushrooms known as “Kabute” in Tagalog,
offer a long list of health benefits. Essentially
it is neither a plant nor an animal. It is a
fungus, hence the group name. Although
mushrooms may not be a number one menu
favorite, you may think twice about pushing
them aside during your next encounter.
White Oyster Mushroom (Ple uro tus
O stre atus)
Most common and
available mushroom in
the Philippines and easy
to cultivate. Oyster
mushrooms are usually
processed into a variety
of delicious food. 
White Oyster Mushroom (Ple uro tus
O stre atus)
• ANTIOXIDANT EFFECTS
Oyster mushrooms contain ergothioneine,
a unique antioxidant exclusively
produced by fungi that fight cancer
tumors
• ANTI-BACTERIAL EFFECTS
• NUTRITIONAL VALUE
Oyster mushrooms are high in nutrients
that contain significant levels of zinc,
iron, potassium, calcium, phosphorus,
vitamin C, folic acid, niacin, and
vitamins B-1 and B-2. Natural source
of Vitamin D.
Agricultural Waste
Preparation of Agar Medium (PDA)
Potato, Dextrose, Agar
Materials:
•Casserole/ 1000 milliliter beaker
•Knife
•Flat bottles or petri dish
•Funnel
•Cotton
•Stirrer
•Stove
•Clean filter cloth
•Pressure cooker
•Heat-protected gloves
•Weighing scale
Ingredients:
•Potato 250 grams
•Dextrose powder 25 grams
•2 Agar bar/Powder 20 grams
•Clean Water 1 liter
Steps in PDA Preparation
Prepare materials
Wash potatoes, peel and cut
into cubes
Boil for 10 minutes Filter or separate potatoes
Add clean water Pour into a boiling pot
Prepare glucose and agar bar/powder Add agar powder/bar
Boil until it is cooked Add in glucose/dextrose powder
Stir well for 10 minutes Add clean water
Pour to 1/4 of a bottle Plug with cotton balls
Bind with rubber bands Put bottles in pressure cooker
Steam under the pressure at
18 PSI for 30 min
Remove from pressure cooker.
Lean the bottles and store.
18 PSI18 PSI
Tissue Culturing (F0 and F1 Sub Cultures)
Clean hands and body
Clean the PDA bottles Select the best mushroom
Clean chamber and equipment
Hold over the fire Tear the mushroom
Cut mushroom tissues Hold the bottle’s opening over flame
Put the tissue in the middle of PDA Plug with cotton balls
Bind paper cap with rubber bands Isolate, store and wait for 14 days
Grain Spawn
Soak sorghum seeds in water for 12 hours Boil the seeds for 15 min
Spread out until half dry Pour into bottles
Plug with cotton balls, wrap with paper and bind with rubber bands
Place bottles in the pressure
cooker
Steam under pressure
of 18 PSI for 1 hour
18 PSI18 PSI
Remove from pressure cooker to cool down Prepare equipment
Clean the needle Unplug the PDA media of Mycelium
Get a small piece of agar. Place
opening of bottle over the flame
Put agar on the seeds, cover with cotton
and bind with paper and rubber
Store in the closet, away from light. Label the bottle with date
and type of mushroom
Once it’s fully colonized, it is ready for grain multiplication and inoculation
Substrate or Fruiting Bag
Spread out sawdust and mix with supplements like lime, sugar, and rice bran
Pour water and mix well Dampness test by hand
Use 6x12 PP plastic Fill with sawdust
Weigh
Compress until firm
Insert PVC around the neck Plug with cotton balls
Cover with paper and rubber band Arrange bags inside the drum
Inoculation or Planting grain
spawn
Materials
Sterilized Substrate or Fruiting
Bag
Planting Grain Spawn
Transfer Needle
Alcohol Lamp
Cotton
Rubber Band
Pour water into the drum
(7 inches high) and steam for 4
hours
Clean picking tool and hands Put opening of bottle and pick
over the flame
Pour 15-20 grain spawn seedsUse needle and shake to
spread spawn seeds
Put cotton stopper back on Incubate bags for 4 to 5 weeks
2 weeks incubation 4 to 5 weeks incubation ready to fruit
Lying on its side, stack the bags vertically in the growing room
Screen Door Area
Wooden Door
3 feet
Walking
space
2 feet walking space
3 feet
Walking
space
1 foot wide gravel
5 Meters (16.4 feet)
8.5 Meters
(25 feet)
Mushroom Barn
Packaging
PROCESSING
• Mushroom Burger Patty
• Dried Mushrooms Powder Salt/Seasoning
• Mushroom Siomai/Dumplings
• Pickled Mushrooms
• Mushroom Siopao, Muffins
• Mushroom Crackers
• Crispy Mushrooms, etc.
MARKETING
COLLABORATION
OPPORTUNITIES
Directly
Retail
Distributor
Website
Super market and
Public Market
Restaurants
Processed Goods
Permission to use the picture; granted by Ping Joven of Ping Gu Mushroom fries.
DEMAND
• According to a DA-Central Luzon
Integrated Agricultural Research Center
(CLIARC) report.
The country is estimated to import around
150 metric tons (MT) of mushrooms
yearly, largely from South East Asia—
Taiwan, China, Thailand, and Japan.
Read more at BusinessDiary.com.ph: 
http://businessdiary.com.ph/5522/da-puts-up-p8-million-mushroom-technology-center-in-tarlac-to-boost-production-substitu
Crispy OysterMushrooms
Ingredients:
• Oyster Mushroom
• Flour
• Salt
• Pepper
• Oil
Procedure:
1. Wash the oyster mushroom, remove water, set aside
2. Add flour (season with salt & pepper. You may also try
breading mix like “Crispy Fry”)
3. Heat up the oil on wok
4. Deep fry till golden brown.
5. Remove and let excess oil drip over a strainer or paper
towel.
6. Serve with Thai chili sauce (optional)
Mushroom Siomai
LIKE US!
/PerfectMushrooms
www.perfectmushrooms.ph
@PerfectMushrooms
09177685515 / 02-7766173

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Mr. Marco Lobregat - The Modern AgriBusiness and its Sustainability

  • 1. MARCO LOBREGATMarketing & Communication Strategist Food & Tourism Advocate Hypersocial Entrepreneur facebook.com/marc o.lobregat @ministryshrooms @mlobregat
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  • 5. Objective • Knowledge about Mushrooms and Health Benefits. • Know the step by step Mushroom Culture and Propagation, Bagging, Harvesting, Processing and Marketing • Appreciate mushrooms as a sustainable livelihood and alternative to meat. • Apply the technology as you will be encouraged to grow mushrooms.
  • 6. Mushrooms Mushrooms known as “Kabute” in Tagalog, offer a long list of health benefits. Essentially it is neither a plant nor an animal. It is a fungus, hence the group name. Although mushrooms may not be a number one menu favorite, you may think twice about pushing them aside during your next encounter.
  • 7.
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  • 9. White Oyster Mushroom (Ple uro tus O stre atus) Most common and available mushroom in the Philippines and easy to cultivate. Oyster mushrooms are usually processed into a variety of delicious food. 
  • 10. White Oyster Mushroom (Ple uro tus O stre atus) • ANTIOXIDANT EFFECTS Oyster mushrooms contain ergothioneine, a unique antioxidant exclusively produced by fungi that fight cancer tumors • ANTI-BACTERIAL EFFECTS • NUTRITIONAL VALUE Oyster mushrooms are high in nutrients that contain significant levels of zinc, iron, potassium, calcium, phosphorus, vitamin C, folic acid, niacin, and vitamins B-1 and B-2. Natural source of Vitamin D.
  • 12.
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  • 14. Preparation of Agar Medium (PDA) Potato, Dextrose, Agar Materials: •Casserole/ 1000 milliliter beaker •Knife •Flat bottles or petri dish •Funnel •Cotton •Stirrer •Stove •Clean filter cloth •Pressure cooker •Heat-protected gloves •Weighing scale Ingredients: •Potato 250 grams •Dextrose powder 25 grams •2 Agar bar/Powder 20 grams •Clean Water 1 liter
  • 15. Steps in PDA Preparation Prepare materials Wash potatoes, peel and cut into cubes
  • 16. Boil for 10 minutes Filter or separate potatoes Add clean water Pour into a boiling pot
  • 17. Prepare glucose and agar bar/powder Add agar powder/bar Boil until it is cooked Add in glucose/dextrose powder
  • 18. Stir well for 10 minutes Add clean water Pour to 1/4 of a bottle Plug with cotton balls
  • 19. Bind with rubber bands Put bottles in pressure cooker Steam under the pressure at 18 PSI for 30 min Remove from pressure cooker. Lean the bottles and store. 18 PSI18 PSI
  • 20. Tissue Culturing (F0 and F1 Sub Cultures)
  • 21. Clean hands and body Clean the PDA bottles Select the best mushroom Clean chamber and equipment
  • 22. Hold over the fire Tear the mushroom Cut mushroom tissues Hold the bottle’s opening over flame
  • 23. Put the tissue in the middle of PDA Plug with cotton balls Bind paper cap with rubber bands Isolate, store and wait for 14 days
  • 25. Soak sorghum seeds in water for 12 hours Boil the seeds for 15 min Spread out until half dry Pour into bottles
  • 26. Plug with cotton balls, wrap with paper and bind with rubber bands Place bottles in the pressure cooker Steam under pressure of 18 PSI for 1 hour 18 PSI18 PSI
  • 27. Remove from pressure cooker to cool down Prepare equipment Clean the needle Unplug the PDA media of Mycelium
  • 28. Get a small piece of agar. Place opening of bottle over the flame Put agar on the seeds, cover with cotton and bind with paper and rubber Store in the closet, away from light. Label the bottle with date and type of mushroom
  • 29. Once it’s fully colonized, it is ready for grain multiplication and inoculation
  • 31. Spread out sawdust and mix with supplements like lime, sugar, and rice bran Pour water and mix well Dampness test by hand
  • 32. Use 6x12 PP plastic Fill with sawdust Weigh Compress until firm
  • 33. Insert PVC around the neck Plug with cotton balls Cover with paper and rubber band Arrange bags inside the drum
  • 34. Inoculation or Planting grain spawn Materials Sterilized Substrate or Fruiting Bag Planting Grain Spawn Transfer Needle Alcohol Lamp Cotton Rubber Band Pour water into the drum (7 inches high) and steam for 4 hours
  • 35. Clean picking tool and hands Put opening of bottle and pick over the flame Pour 15-20 grain spawn seedsUse needle and shake to spread spawn seeds
  • 36. Put cotton stopper back on Incubate bags for 4 to 5 weeks 2 weeks incubation 4 to 5 weeks incubation ready to fruit
  • 37.
  • 38. Lying on its side, stack the bags vertically in the growing room
  • 39. Screen Door Area Wooden Door 3 feet Walking space 2 feet walking space 3 feet Walking space 1 foot wide gravel 5 Meters (16.4 feet) 8.5 Meters (25 feet)
  • 42. PROCESSING • Mushroom Burger Patty • Dried Mushrooms Powder Salt/Seasoning • Mushroom Siomai/Dumplings • Pickled Mushrooms • Mushroom Siopao, Muffins • Mushroom Crackers • Crispy Mushrooms, etc.
  • 43.
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  • 54.
  • 55. OPPORTUNITIES Directly Retail Distributor Website Super market and Public Market Restaurants Processed Goods Permission to use the picture; granted by Ping Joven of Ping Gu Mushroom fries.
  • 56. DEMAND • According to a DA-Central Luzon Integrated Agricultural Research Center (CLIARC) report. The country is estimated to import around 150 metric tons (MT) of mushrooms yearly, largely from South East Asia— Taiwan, China, Thailand, and Japan. Read more at BusinessDiary.com.ph:  http://businessdiary.com.ph/5522/da-puts-up-p8-million-mushroom-technology-center-in-tarlac-to-boost-production-substitu
  • 57. Crispy OysterMushrooms Ingredients: • Oyster Mushroom • Flour • Salt • Pepper • Oil Procedure: 1. Wash the oyster mushroom, remove water, set aside 2. Add flour (season with salt & pepper. You may also try breading mix like “Crispy Fry”) 3. Heat up the oil on wok 4. Deep fry till golden brown. 5. Remove and let excess oil drip over a strainer or paper towel. 6. Serve with Thai chili sauce (optional)
  • 58.
  • 60.