This document discusses various topics related to DNA replication and genetic diseases. It describes how origin of replication (ORI) proteins recognize the origin of replication site to initiate DNA duplication. It also discusses alternative treatments for diseases caused by parasites like Trypanosoma brucei that cause sleeping sickness. Finally, it mentions how understanding genetic causes of diseases like Meier-Gorlin syndrome could allow for preventative genetic therapies in the future.

1. Carolina Jaramillo Mesa
Medical student UPB
ID: 00012039
Molecular biology
August 6 2012

5. Recognition of ORI (Origin
of replication) Mutation
Helicase
DNA unroll
DNA polymerase
DNA replicate
Reparation of
DNA
Conservation and
transmition of the DNA

7.  Trypanosoma brucei it’s a parasite type
kinetoplastia, wich have the mtDNA encapsulated in
the mithocondrion with many copies of the
mitochondrial genome. This parasite cause African
sleeping sickness.
Taken from: http://www.microbiologyonline.org.uk/about-
microbiology/introducing-microbes/protozoa

8. Taken from:
http://www.parasitemuseum.com/trypanosome/
DNA Copy the Daughter
DNA circles cells
polymerase information

9. Relationship with
replicating kDNA
DNA polymerase ID
molecules
Experiment Localization of DNA
Inmunofluorescence polymerase ID

10.  This parasites cant be eradicate with antibiotics by the
complex mitochondrial genome and the only way to kill
them is using toxics. This research proposed a alternative
treatments more effectivs and less agresives.

12.  Meier-Gorlin
syndrome : it is
characterized for
severe growth
defects, skeletal
abnormalities and
small brain size.
Taken from
http://abbottgenetics.wordpress.com/2009/03/09/
why-the-littlest-angel-was-so-little-by-bryan-cooke/

13.  ORC recognize the ORI to start the DNA
replication and coordinate that the
genetic material duplicate just once. Also
ensure the correct duplication of the
genetic material through centrosome.
Taken from: http://www.intechopen.com/books/dna-replication-current-
advances/the-silencing-face-of-dna-replication-gene-repression-mediated-by-
dna-replication-factors

14. pre-RC Regulation of
DNA the
recognize the replicaction
ORI centrosome
alteration of the
Orc1-encoding Reduplication of
cell cycle and
gene is mutated the centrosome
cell signaling

15.  Knowing the causes of the disease allow us
treat this mutation before its expression,
through genetic therapy with the objective
to prevent the damage and in a future
decrease the number of kids with Meier
Gorlin syndrome.
Taken from: http://www.fedaes.es/FOLLFA/B14-TERA.htm

17.  This research expose us alternative
treatments to diseases as complicated as
those caused by parasites, African sleeping
sickness, Chagas disease and leishmaniasis
and understand the cellular processes that
take place in these diseases.

18.  The genetic diseases have increased with the
pass of the years, they are misunderstood
because the cause of them are unknown in the
most part of cases and the treatment are very
limited, so as a doctors is very important inform
about this diseases and exposed our patients the
alternatives treatments and the explain them all
about this diseases.
Taken from:
http://www.sitiosespana.com/notas/2010/mayo/oligof
renia.htm

19.  MARTINEZ, Lina; Biologia molecular, Vol 6.
ed. UPB, 2011. Paginas 74-81.
 Secrets of Parasites' Replication Unraveled;
Sciencedaily; july 9 2012;
http://www.sciencedaily.com/releases/2012
/07/120710141940.htm
 Protein Involved in DNA Replication,
Centrosome Regulation Linked to Dwarfism,
Small Brain Size; Sciencedaily; July 31 2012;
http://www.sciencedaily.com/releases/2012
/07/120731200628.htm