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Please answer all the questions. Please answer only if you know how to it. Don't just answer 1
question and submit it. This is one part with multiple questions. If you can, please explain how
you get the answer if possible. If you are refering to question 10, skip part a Class Dala YEPD
plati Strains of S ra in r op muti, muting type type pln) Plate Strui, r o mutiny Struts of muting t
pe ple al a 2 a a a a
Solution
YEPD do not show any complementation. Ym plate shows complementation. - And – is no
complementation. On the same gene. + and – is complementation. On the different gene. - +- and
+-+ is complementation. -+- and -+- is no complementation. Thus on Ym plate four
complementation groups observed. a1 shows -+, a1 complement a2,a4,a5,a6 and a3. So, a1 is on
different gene than a2,a4,a6 and a3. a2 and a5 shows +-+, a2 do not complement a5, so they are
on same gene. a3,a4 and a6 shows ++-, a4 do not complement a6, so they are on same gene. a3
do not complement a4 and a6 shows all the three mutations are on the same gene. Thus three
complementation groups have been observed. There are at least three genes have been observed
in Ym. Complementation test thus helpful in finding whether the mutations in these strains are in
the same gene or in diffrent genes. If the mutations are in the same genes, the complementation
will not occur. Thus a1 complement a5 shows these mutations have been on diffrent genes.

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Please answer all the questions. Please answer only if you know how .pdf

  • 1. Please answer all the questions. Please answer only if you know how to it. Don't just answer 1 question and submit it. This is one part with multiple questions. If you can, please explain how you get the answer if possible. If you are refering to question 10, skip part a Class Dala YEPD plati Strains of S ra in r op muti, muting type type pln) Plate Strui, r o mutiny Struts of muting t pe ple al a 2 a a a a Solution YEPD do not show any complementation. Ym plate shows complementation. - And – is no complementation. On the same gene. + and – is complementation. On the different gene. - +- and +-+ is complementation. -+- and -+- is no complementation. Thus on Ym plate four complementation groups observed. a1 shows -+, a1 complement a2,a4,a5,a6 and a3. So, a1 is on different gene than a2,a4,a6 and a3. a2 and a5 shows +-+, a2 do not complement a5, so they are on same gene. a3,a4 and a6 shows ++-, a4 do not complement a6, so they are on same gene. a3 do not complement a4 and a6 shows all the three mutations are on the same gene. Thus three complementation groups have been observed. There are at least three genes have been observed in Ym. Complementation test thus helpful in finding whether the mutations in these strains are in the same gene or in diffrent genes. If the mutations are in the same genes, the complementation will not occur. Thus a1 complement a5 shows these mutations have been on diffrent genes.