You are working In a lab with several different macromolecules from Francisella tulameria a bacterial species that causes an illness similar to bubonic plague. You drop the lest tube rack containing purified metabolic enzymes, plasma membranes, chromosomes, and cell walls Unfortunately your labels also fell off when the tubes hit the floor. Your lab can detect pentose, hexose, adenine, cytosine, cysteine. and glycerol (AH your other testing equipment is beyond your training and you don\'t want your boss to know you dropped test tubes In lab (bad call, especially r a lab. while working with a biohazardous organism)) What test would you use to differentiate between the tube with DMA and the tube with proteins? What test would you use to differentiate between tubes with carbohydrates and those with DNA? What test would you use to identify the test tube with the plasma membranes\' Fill in the cell structures with an A-D Metabolic enzymes Plasma membranes Chromosomes Cell Walls In the last 20 years, there has been a dramatic increase in the incidence of fluoroquinolone resistant gram negative bacteria in patient samples. Fluoroquinolones are antibiotics (like Cipro and Levaquin) that bind to and inactivate gyrase. Gyrase is an enzyme that is needed to relieve the tension formed by opening a replication fork on both sides of a replication bubble DNA replication of circular DNA. Without functional gyrase. DNA cannot be copied in the affected cells. Researchers have isolated and sequenced the DNA coding part of the gyrase enzyme (gyrA) and found that resistant strains of bacteria typically have mutations that cause them to substitute one amino acid for another. Based on your reading of section A1.3 and review of Module 3 s notes and study guide, predict which alterations would lead to resistance and which would lead to cell death. Explain. Solution We use adenine +cytosine and cystiene detection test perform for DNA and protein. Adenine and cytosine are found in DNA and cystiene is found in protein as making disulphide bridges. So we take 10-15 ul of each sample in different vials and add detection test enzymes of both on each vial. Then we can detect via observing optical density. For carbohydrate and DNA we take hexose (for detecting carbohydrate) and adenine +cytosine for DNA. For plasma membrane, glycerol detection test is required. Glycerol is a key constitute of membrane..

1. You are working In a lab with several different macromolecules from Francisella tulameria a
bacterial species that causes an illness similar to bubonic plague. You drop the lest tube rack
containing purified metabolic enzymes, plasma membranes, chromosomes, and cell walls
Unfortunately your labels also fell off when the tubes hit the floor. Your lab can detect pentose,
hexose, adenine, cytosine, cysteine. and glycerol (AH your other testing equipment is beyond
your training and you don't want your boss to know you dropped test tubes In lab (bad call,
especially r a lab. while working with a biohazardous organism)) What test would you use to
differentiate between the tube with DMA and the tube with proteins? What test would you use
to differentiate between tubes with carbohydrates and those with DNA? What test would you
use to identify the test tube with the plasma membranes' Fill in the cell structures with an A-D
Metabolic enzymes Plasma membranes Chromosomes Cell Walls In the last 20 years, there
has been a dramatic increase in the incidence of fluoroquinolone resistant gram negative bacteria
in patient samples. Fluoroquinolones are antibiotics (like Cipro and Levaquin) that bind to and
inactivate gyrase. Gyrase is an enzyme that is needed to relieve the tension formed by opening a
replication fork on both sides of a replication bubble DNA replication of circular DNA. Without
functional gyrase. DNA cannot be copied in the affected cells. Researchers have isolated and
sequenced the DNA coding part of the gyrase enzyme (gyrA) and found that resistant strains of
bacteria typically have mutations that cause them to substitute one amino acid for another. Based
on your reading of section A1.3 and review of Module 3 s notes and study guide, predict which
alterations would lead to resistance and which would lead to cell death. Explain.
Solution
We use adenine +cytosine and cystiene detection test perform for DNA and protein. Adenine and
cytosine are found in DNA and cystiene is found in protein as making disulphide bridges. So we
take 10-15 ul of each sample in different vials and add detection test enzymes of both on each
vial. Then we can detect via observing optical density.
For carbohydrate and DNA we take hexose (for detecting carbohydrate) and adenine +cytosine
for DNA.
For plasma membrane, glycerol detection test is required. Glycerol is a key constitute of
membrane.