Polymerase chain reaction (PCR) is a process used to replicate DNA in vitro. DNA replication is the biological process by which original DNA molecule replicate in to two identical replicas of DNA. DNA polymerase III replicate the Prokaryotic DNA in 5\' to 3\' at a rate of 1 kb/s but 1-4 kb/min in a typical PCR reaction. Prokaryotic DNA is circular and replication starts at single origin (ori C). It do not occur in PCR. Prokaryotic DNA replication is followed by the three basic step:- 1. initiation, 2. elongation, and 3. termination. The process of PCR is followed by the three basic step:- 1. Denaturation, 2.,Annealing, and 3. Primer Extension 1) the process of forming single stranded DNA, :-In PCR the DNA denatures at high tempreture, at 95oC to form its single-stranded. During the prokaryotic DNA Replication, Ori C unwinds the DNA by DNA B or helicase, and extension of ssDNA for copying during initiation step. 2) priming of the DNA polymerase,:-In PCR primer binds with the complementry strand of DNA during the Annealing step. In prokaryotic replcation, primase form RNA primer extended by DNA polymerase III,during the elongation step. Synthesis ofleading strand and lagging strand takes place in this step. It do not occur in PCR. 3) the DNA polymerase itself:-In prokaryotic DNA replication DNA polymerase III synthesize the segment of DNA for both leading and lagging strand. In PCR, DNA polymerase extends the primer, Taq polymerase, is used in this process and extension is performed at 72oC. 4) the end product:- As a PCR two ds DNA is formed. And in prokaryotic DNA replication 2 circular DNA is formed , which remain interlinked and topoisomerase II make them seprate by making cut over them. Protien involed in prokaryotic DNA replication:- Dna A protein-It initiate DNA replication by the binding with the Dna A boxes. Dna helicase- it seprates the ds DNA. Topoisomerase- From the replication fork, it removes positive supercoiling. Single stranded binding protein-to prevent the reformation of ds DNA, it binds with single stranded structure, primase - synthesis the short RNA primer. DNA polymerase III-In the leading and lagging strands, it synthesize the DNA. DNA polymerase I- Removes RNA primer DNA ligase- it joins the fragments. Tus;-binds with ter sequence. DNA replication different than PCR:--PCR generates large quantities of DNA from very small amount of DNA, PCR is a INVITRO technique, while DNA replication occurs inside the iving system, it is a invivo technique. The whole DNA is replicated, in DNA replication, while in PCR the amplification of segment of DNA takes place. Solution Polymerase chain reaction (PCR) is a process used to replicate DNA in vitro. DNA replication is the biological process by which original DNA molecule replicate in to two identical replicas of DNA. DNA polymerase III replicate the Prokaryotic DNA in 5\' to 3\' at a rate of 1 kb/s but 1-4 kb/min in a typical PCR reaction. Prokaryotic DNA is circular and replication st.

1. Polymerase chain reaction (PCR) is a process used to replicate DNA in vitro. DNA replication is
the biological process by which original DNA molecule replicate in to two identical replicas of
DNA. DNA polymerase III replicate the Prokaryotic DNA in 5' to 3' at a rate of 1 kb/s but 1-4
kb/min in a typical PCR reaction. Prokaryotic DNA is circular and replication starts at single
origin (ori C). It do not occur in PCR.
Prokaryotic DNA replication is followed by the three basic step:-
1. initiation, 2. elongation, and 3. termination.
The process of PCR is followed by the three basic step:-
1. Denaturation, 2.,Annealing, and 3. Primer Extension
1) the process of forming single stranded DNA, :-In PCR the DNA denatures at high tempreture,
at 95oC to form its single-stranded. During the prokaryotic DNA Replication, Ori C unwinds the
DNA by DNA B or helicase, and extension of ssDNA for copying during initiation step.
2) priming of the DNA polymerase,:-In PCR primer binds with the complementry strand of DNA
during the Annealing step. In prokaryotic replcation, primase form RNA primer extended by
DNA polymerase III,during the elongation step. Synthesis ofleading strand and lagging strand
takes place in this step. It do not occur in PCR.
3) the DNA polymerase itself:-In prokaryotic DNA replication DNA polymerase III synthesize
the segment of DNA for both leading and lagging strand. In PCR, DNA polymerase extends the
primer, Taq polymerase, is used in this process and extension is performed at 72oC.
4) the end product:- As a PCR two ds DNA is formed. And in prokaryotic DNA replication 2
circular DNA is formed , which remain interlinked and topoisomerase II make them seprate by
making cut over them.
Protien involed in prokaryotic DNA replication:-
Dna A protein-It initiate DNA replication by the binding with the Dna A boxes.
Dna helicase- it seprates the ds DNA.
Topoisomerase- From the replication fork, it removes positive supercoiling.
Single stranded binding protein-to prevent the reformation of ds DNA, it binds with single
stranded structure,
primase - synthesis the short RNA primer.
DNA polymerase III-In the leading and lagging strands, it synthesize the DNA.
DNA polymerase I- Removes RNA primer
DNA ligase- it joins the fragments.
Tus;-binds with ter sequence.
DNA replication different than PCR:--PCR generates large quantities of DNA from very small
amount of DNA, PCR is a INVITRO technique, while DNA replication occurs inside the iving

2. system, it is a invivo technique. The whole DNA is replicated, in DNA replication, while in PCR
the amplification of segment of DNA takes place.
Solution
Polymerase chain reaction (PCR) is a process used to replicate DNA in vitro. DNA replication is
the biological process by which original DNA molecule replicate in to two identical replicas of
DNA. DNA polymerase III replicate the Prokaryotic DNA in 5' to 3' at a rate of 1 kb/s but 1-4
kb/min in a typical PCR reaction. Prokaryotic DNA is circular and replication starts at single
origin (ori C). It do not occur in PCR.
Prokaryotic DNA replication is followed by the three basic step:-
1. initiation, 2. elongation, and 3. termination.
The process of PCR is followed by the three basic step:-
1. Denaturation, 2.,Annealing, and 3. Primer Extension
1) the process of forming single stranded DNA, :-In PCR the DNA denatures at high tempreture,
at 95oC to form its single-stranded. During the prokaryotic DNA Replication, Ori C unwinds the
DNA by DNA B or helicase, and extension of ssDNA for copying during initiation step.
2) priming of the DNA polymerase,:-In PCR primer binds with the complementry strand of DNA
during the Annealing step. In prokaryotic replcation, primase form RNA primer extended by
DNA polymerase III,during the elongation step. Synthesis ofleading strand and lagging strand
takes place in this step. It do not occur in PCR.
3) the DNA polymerase itself:-In prokaryotic DNA replication DNA polymerase III synthesize
the segment of DNA for both leading and lagging strand. In PCR, DNA polymerase extends the
primer, Taq polymerase, is used in this process and extension is performed at 72oC.
4) the end product:- As a PCR two ds DNA is formed. And in prokaryotic DNA replication 2
circular DNA is formed , which remain interlinked and topoisomerase II make them seprate by
making cut over them.
Protien involed in prokaryotic DNA replication:-
Dna A protein-It initiate DNA replication by the binding with the Dna A boxes.
Dna helicase- it seprates the ds DNA.
Topoisomerase- From the replication fork, it removes positive supercoiling.
Single stranded binding protein-to prevent the reformation of ds DNA, it binds with single
stranded structure,
primase - synthesis the short RNA primer.
DNA polymerase III-In the leading and lagging strands, it synthesize the DNA.
DNA polymerase I- Removes RNA primer

3. DNA ligase- it joins the fragments.
Tus;-binds with ter sequence.
DNA replication different than PCR:--PCR generates large quantities of DNA from very small
amount of DNA, PCR is a INVITRO technique, while DNA replication occurs inside the iving
system, it is a invivo technique. The whole DNA is replicated, in DNA replication, while in PCR
the amplification of segment of DNA takes place.