Prokaryotic DNA replication begins at the origin of replication site where the replication fork is formed. DnaA and other proteins recognize the origin site and unwind the DNA double helix. RNA primers are synthesized which allows DNA polymerase III to begin bidirectional DNA synthesis of the leading and lagging strands. Okazaki fragments are formed and ligated on the lagging strand. Replication terminates when the replication forks meet at the termination site and the daughter chromosomes are separated.