Not all analytes re non-polar hydrophobic substance, but even those are detected by allowing them to be bound to beads with which they show affinity. The different weights added by the beads allows to monitor their elution fairly one-at-a-time, necessary for a non-chaotic non-contaminated chromatogram. Substances that are amphoteric like proteins, with one ionizable motif, one polar motif, and one hydrophobic motif are detected by their anion and cation exchange pattern, and so are large molecules that are polar and or ionizable. The different affinity to one ion or the next and their tendency to exchange cations or anions is part of the information. If some of those substances are biologically, they can adopt several conformations. The one they adopt in living systems i usually the active one, and in that conformation they interact in nature with the ions and hydrophylic proteins of our bodies\' liquid compartments. Also information is derived from their affinity to the other substances, the beads, the eluents, and the ions, and the overall pH. Solution Not all analytes re non-polar hydrophobic substance, but even those are detected by allowing them to be bound to beads with which they show affinity. The different weights added by the beads allows to monitor their elution fairly one-at-a-time, necessary for a non-chaotic non-contaminated chromatogram. Substances that are amphoteric like proteins, with one ionizable motif, one polar motif, and one hydrophobic motif are detected by their anion and cation exchange pattern, and so are large molecules that are polar and or ionizable. The different affinity to one ion or the next and their tendency to exchange cations or anions is part of the information. If some of those substances are biologically, they can adopt several conformations. The one they adopt in living systems i usually the active one, and in that conformation they interact in nature with the ions and hydrophylic proteins of our bodies\' liquid compartments. Also information is derived from their affinity to the other substances, the beads, the eluents, and the ions, and the overall pH..

1. Not all analytes re non-polar hydrophobic substance, but even those are detected by
allowing them to be bound to beads with which they show affinity. The different weights added
by the beads allows to monitor their elution fairly one-at-a-time, necessary for a non-chaotic
non-contaminated chromatogram. Substances that are amphoteric like proteins, with one
ionizable motif, one polar motif, and one hydrophobic motif are detected by their anion and
cation exchange pattern, and so are large molecules that are polar and or ionizable. The different
affinity to one ion or the next and their tendency to exchange cations or anions is part of the
information. If some of those substances are biologically, they can adopt several conformations.
The one they adopt in living systems i usually the active one, and in that conformation they
interact in nature with the ions and hydrophylic proteins of our bodies' liquid compartments.
Also information is derived from their affinity to the other substances, the beads, the eluents, and
the ions, and the overall pH.
Solution
Not all analytes re non-polar hydrophobic substance, but even those are detected by
allowing them to be bound to beads with which they show affinity. The different weights added
by the beads allows to monitor their elution fairly one-at-a-time, necessary for a non-chaotic
non-contaminated chromatogram. Substances that are amphoteric like proteins, with one
ionizable motif, one polar motif, and one hydrophobic motif are detected by their anion and
cation exchange pattern, and so are large molecules that are polar and or ionizable. The different
affinity to one ion or the next and their tendency to exchange cations or anions is part of the
information. If some of those substances are biologically, they can adopt several conformations.
The one they adopt in living systems i usually the active one, and in that conformation they
interact in nature with the ions and hydrophylic proteins of our bodies' liquid compartments.
Also information is derived from their affinity to the other substances, the beads, the eluents, and
the ions, and the overall pH.