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the initiation phase of eukaryotic transcription via RNA polymerase ll is considered an assembly
and disassembly C24. The initiation phase of eukaryotic transcription via RNA poly- merase II is
considered an assembly and disassembly process. Which types of biochemical interactions-
hydrogen bonding, ionic bonding, covalent bonding, and/or hydrophobic interactions would you
expect to drive the assembly and disassembly process? How would temperature and salt
concentration affect assembly and disassembly?
Solution
During the assembly and disassembly the Hydrogen bond between the DNA strand and the RNA
polymerase II is predominantly involved. The ionic and hydrophobic interactions may occur
depending on the sequence and the supercoiling of the template DNA. Since assembly and
disassembly are dynamic process, the stronger covalent bonds are not involved, which are
difficult to break.
The hydrogen bonds are broken down easily in high temperature and high salt concentrations.
Hence these conditions would inhibit the process of RNApol assembly and disassembly.

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the initiation phase of eukaryotic transcription via RNA polymerase .pdf

  • 1. the initiation phase of eukaryotic transcription via RNA polymerase ll is considered an assembly and disassembly C24. The initiation phase of eukaryotic transcription via RNA poly- merase II is considered an assembly and disassembly process. Which types of biochemical interactions- hydrogen bonding, ionic bonding, covalent bonding, and/or hydrophobic interactions would you expect to drive the assembly and disassembly process? How would temperature and salt concentration affect assembly and disassembly? Solution During the assembly and disassembly the Hydrogen bond between the DNA strand and the RNA polymerase II is predominantly involved. The ionic and hydrophobic interactions may occur depending on the sequence and the supercoiling of the template DNA. Since assembly and disassembly are dynamic process, the stronger covalent bonds are not involved, which are difficult to break. The hydrogen bonds are broken down easily in high temperature and high salt concentrations. Hence these conditions would inhibit the process of RNApol assembly and disassembly.