Autodisplay Biotech GmbH - We Make Screening Easy

•

0 likes•2,494 views

If screening applications resemble a scientific or economic bottleneck in your company, a very unique approach can help: Autodisplay Biotech GmbH's proprietary technologies pMATE and Autodisplay enable the development of cost-efficient screening tools, which can be used for the identification of binding partners such as inhibitors, activators, biomarkers or autoantigens. We use fluorescence marked libraries in order to identify targets. Any binding activity can then be selected through fluorescence activated cell sorting (FACS). Find out how our custom-tailored products and services can assist in bringing your research forward.

Technology Business

are
you

ENGAGED IN
SCREENING ACTIVITIES?

SCREENING CAN BE SO MUCH
EASIER WITH …

bacterial
surface
display

OUR TECHNOLOGY…

OM

PP

IM
CP

SP

PASSENGER

LINKER

β-BARREL

… IS A SELF-SUFFICIENT SYSTEM

OM

PP

IM
CP

SP

PASSENGER

LINKER

β-BARREL

SURFACE DISPLAY ENABLES A
SIMPLE APPROACH TO SCREENING
create
diversity

use a
library

display the
library

display the
library

display the
target

analyse

analyse

ACTIVITY SCREENING

BINDING INTERACTIONS

SPEED UP YOUR SCREENING FOR
BINDING INTERACTION WITH FACS

1

2

PEPTIDE OR PROTEIN

CREATE DIVERSITY

PREPARE SAMPLE

LIBRARY DISPLAY AND USE OF A
FLUORESCING TARGET STRUCTURE
STREPTAVIDIN
FITC

FLUORESCENT
MARKER

INHIBITOR
PRODUCT

WASTE

CATCHER TUBE
LASER

BIOTIN MARKED
TARGET MOLECULE

3

SCREEN WITH FACS
(OPTION A)

PROBE INJECTION

LIBRARY DISPLAY AND USE OF A
DISPLAYED TARGET
FLUORESCENT CELL
DISPLAYING TARGET
INHIBITOR
PRODUCT

WASTE

CATCHER TUBE
LASER

TARGET MOLECULE
PROBE INJECTION

3

SCREEN WITH FACS
(OPTION B)

SIMPLIFY YOUR ENZYME
OPTIMIZATION PROCESSES
NO MORE
PURIFICATION
OF TARGET

&
NO MORE
USE OF CELL
LYSATES
PEPTIDE OR PROTEIN

DETERMINE ACTIVITY
STRAIGHT AWAY

HELP

WE CAN
, WHEN
OTHER SYSTEMS FAIL

ESPECIALLY IN THE CASE OF

CHALLENGING ENZYMES

you
may
ask

WHAT ARE
CHALLENGING
ENZYMES?

ALL ENZYMES WHICH ARE
DIFFICULT TO HANDLE, DUE TO…

inclusion
bodies

incorrect
folding

multimerisation

lack of
stability
co-factors

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1. are you ENGAGED IN SCREENING ACTIVITIES?

2. SCREENING CAN BE SO MUCH EASIER WITH … bacterial surface display

3. OUR TECHNOLOGY… OM PP IM CP SP PASSENGER LINKER β-BARREL

4. … IS A SELF-SUFFICIENT SYSTEM OM PP IM CP SP PASSENGER LINKER β-BARREL

5. SURFACE DISPLAY ENABLES A SIMPLE APPROACH TO SCREENING create diversity use a library display the library display the library display the target analyse analyse ACTIVITY SCREENING BINDING INTERACTIONS

6. WHAT ARE THE BENEFITS

7. SPEED UP YOUR SCREENING FOR BINDING INTERACTION WITH FACS 1 2 PEPTIDE OR PROTEIN CREATE DIVERSITY PREPARE SAMPLE

8. LIBRARY DISPLAY AND USE OF A FLUORESCING TARGET STRUCTURE STREPTAVIDIN FITC FLUORESCENT MARKER INHIBITOR PRODUCT WASTE CATCHER TUBE LASER BIOTIN MARKED TARGET MOLECULE 3 SCREEN WITH FACS (OPTION A) PROBE INJECTION

9. LIBRARY DISPLAY AND USE OF A DISPLAYED TARGET FLUORESCENT CELL DISPLAYING TARGET INHIBITOR PRODUCT WASTE CATCHER TUBE LASER TARGET MOLECULE PROBE INJECTION 3 SCREEN WITH FACS (OPTION B)

10. SIMPLIFY YOUR ENZYME OPTIMIZATION PROCESSES NO MORE PURIFICATION OF TARGET & NO MORE USE OF CELL LYSATES PEPTIDE OR PROTEIN DETERMINE ACTIVITY STRAIGHT AWAY

11. HELP WE CAN , WHEN OTHER SYSTEMS FAIL ESPECIALLY IN THE CASE OF CHALLENGING ENZYMES

12. you may ask WHAT ARE CHALLENGING ENZYMES?

13. ALL ENZYMES WHICH ARE DIFFICULT TO HANDLE, DUE TO… inclusion bodies incorrect folding multimerisation lack of stability co-factors

14. TALK TO US, IF YOU WANT TO KNOW MORE

Autodisplay Biotech GmbH - We Make Screening Easy

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