The Vitek 2 Compact system is an automated microbiology platform for bacterial identification and antimicrobial susceptibility testing, consisting of several key components like a compact workstation, barcode scanner, and test cassettes. Sample preparation involves creating bacterial suspensions and using a DensiChek Plus instrument for turbidity adjustments before placing them into reagent cards for automated analysis. The system features various reagent cards tailored for different organism classes, facilitating both identification and antibiotic susceptibility testing.

1. ADVANCE IDENTIFICATION SYSTEMS:
VITEK 2 COMPACT
Prepared by Abeer Babiker

2. What is VITEK 2 Compact
 The VITEK 2 compact system is
an automated microbiology
bacterial identification and
antimicrobial susceptibility
system.

3. Components
1. VITEK 2 Compact
2. Compact Workstation
3. Barcode Scanner
4. Shared Printer
5. UPS (Uninterruptible
Power Supply)
6. Power Conditioner
7. Test Cassettes
8. DensiCHEK™ Plus

4. VITEK 2 Workflow

5. Material for test
 Culture for patient sample
 Test tube rack
 Slain solution 0.45% PH 4.5-7
 Dispenser
 stick swab
 DensiCHEK plus instrument To measure microorganism
density
 Tube fixed pipetting (red for gram -ve and blue for gram +ve)
 Vortex
 Tissue
 Pastuer pipette
 Powder free gloves
 Biohazard container

6. Sample Preparation
 Bacterial suspensions prepared.
 A sterile swab is used to transfer
a sufficient number of colonies (3)
of a pure culture and to suspend the
microorganism in 3.0 mL of sterile
saline (0.45% NaCl) in a clear plastic
test tube.
 Mix suspension use Vortex until
have homogenous suspension

7.  Put the tube in DensiCHEKplus
then rotate 3600after that
instrument read density
acceptable range 0.5-0.63 for
bacteria and for yeast 1.8-2.2
 The turbidity is adjusted
accordingly Table 1 and
measured by DensiChekTM.
 If the turbidities is less than
range add more bacterial
coloney
 If turbidity is more than range
add more saline 0.45%
 To avoided contamination don't
add solution from dispenser
DensiCHEK™ Plus INSTRUMENT FOR
turbidity adjustment

8. ID and AST pair setup
Tube fixed pipetting (red for gram -ve and
blue for gram +ve) From suspension take
145mm for gram-ve bacteria and 280mm
from gram+ve or yeast Add the above
volume to 3 mm of saline
Vitek Tube Compact Cassette
• The test suspension tube is placed into
the “cassette”, with Reagent card
• Sealed test kits are moved into the
reader/incubator automatically.
• Test kits incubate and analyze
automatically.

9. Reagents Cards
Reagent card – 64 wells with substrates of each test cards.

10.  There are currently four
reagent cards available for
the identification of different
organism classes as follows:
 1. GN - Gram-negative
fermenting and non-
fermenting bacilli.
 2. GP - Gram-positive cocci
and non-spore-forming
bacilli .
 3. YST - yeasts and yeast-like
organisms.
 4. BCL - Gram-positive
spore-forming bacilli.

11.  Antibiotic Susceptibility Testing (AST)
 Gram positive antimicrobial susceptibility testing
(AST) cards.
 Gram negative antimicrobial susceptibility testing
(AST) cards.
 Yeast antimicrobial susceptibility testing (AST) card.

13. VITEK 2 cards

14. Identification cards are inoculated with
microorganism suspensions by vacuum
apparatus