Hairy root culture is a type of plant tissue culture.

1. HAIRY ROOT CULTURE Transformed root culture

2. DEFINITION
Hairy root culture, also called transformed
root culture, is a type of plant tissue
culture that is used to study plant
metabolic processes or to produce
valuable secondary metabolites or
recombinant proteins, often with plant
genetic engineering.

3. AGROBACTERIUM RHIZOGENES

4. METHODS FOR HAIRY ROOT
CULTURE

5. HAIRY ROOT CULTURE
RESEARCH
Some of the applied research on
utilization of hairy root cultures has been
and is conducted at VTT Technical
Research Centre of Finland Ltd. Other labs
working on hairy roots are the
phytotechnology lab of Amiens University
and the Arkansas Biosciences Institute.

6. PHYTOREMEDIATION
Phytoremediation technologies use living
plants to clean up soil, air, and water
contaminated with hazardous
contaminants.

7. METABOLIC STUDIES
Hairy root cultures can be used for
phytoremediation, and are particularly
valuable for studies of the metabolic
processes involved in phytoremediation.

8. GENETICALLY TRANSFORMED
CULTURES
The Ri plasmids can be engineered to also
contain T-DNA, used for genetic
transformation (biotransformation) of the
plant cells. The resulting genetically
transformed root cultures can produce
high levels of secondary metabolites,
comparable or even higher than those of
intact plants.

9. USE IN PLANT PROPAGATION
Hairy root culture can also be used for
regeneration of whole plants and for
production of artificial seeds.

10. ADVANTAGES OF HAIRY
ROOT CULTURE
Harvesting roots for extracting secondary
metabolites can cause destruction to
whole plants. Therefore, interest in
producing secondary metabolites by
developing hairy root culture has been
raised.
Hairy root culture potentially grows faster
without needing an external supply of
auxins. In certain cases, they do not need
incubation under light.

11. GROWTH
• Yield in hairy root cultures can be
altered by optimizing various factors such
as carbon source and its concentration,
ionic concentration of the medium, pH of
the medium, light, temperature, and
inoculum .

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VIGNESH.V