Antiaging Phytosomal Gel

1. DELHI PHARMACEUTICAL SCIENCES AND
RESEARCH UNIVERSITY
FORMULATION AND EVALUATION OF ANTIAGING PHYTOSOMAL GEL
SUPERVISED BY :- PRESENTED BY :-
Dr. MINAKSHI GARG KUMARI SUPRIYA
ASSOCIATE PROFESSOR M.PHARMA COSMECEUTICS
72/MPH/DPSRU/19

2. IMPACT FACTOR –1.23

3. CONTENT
 ABSTRACT
 INTRODUCTION OF AGING
 PHYTOSOMES
 MATERIAL & METHODS
 PROCEDURE
 COMPOSITION
 EVALUATION
 RESULTS
 CONCLUSION
 REFERENCES

4. ABSTRACT
AIM – To prepare and evaluate antiaging phytosomal gel.
METHOD – Coconut water – Cytokinins, Grape seed Extract – Polyphenols and
proanthocyanidin
Aloe Vera Extract – Vitamin C and phenols,
Vitamin E and Jojoba Oil – Antioxidant and Moisturizing properties
Phytosome was prepared by binding herbal extracts to phosphatidylcholine (acts as a carrier
and nourishes the skin). They had a phospholipid molecular structure which included a water-
soluble head and two fat-soluble tails, and due to this dual solubility, it was better absorbed and
so it was used for the treatment of skin disorders, antiaging.

5. RESULT - F2 containing both tender coconut water and A. Vera extract was chosen as the optimized
formula. Optimization was done on the basis of in vitro antioxidant studies and physicochemical
parameters. F2 acts as a potent free radical scavenger and inhibits oxidation by free radicals.
CONCLUSION - From the present study, it can be concluded that the prepared antiaging phytosomal
gel was safe, convenient, and efficient carrier to deliver the herbal extracts. It also showed better
penetration into the skin. Hence, the desired antiaging property was obtained. Hence, the desired
antiaging property was obtained and is used in skin care cosmetics.

6. INTRODUCTION
 Aging is the process of becoming older. In humans, ageing represents the accumulation of
changes in a human being over time and can encompass physical, psychological, and social
changes.
 It is signified by progressive deterioration and degeneration of cells and organs.
 According to biologists, aging is a sum of all changes that occur in a living being with the
passage of time and lead to a decreasing ability to survive stress, to functional impairment, and
finally to death.

7. AETIOLOGY OF SKIN AGING

8. CLASSIFICATION OF AGING

9. 7 SIGNS OF AGING

10. PATHOPHYSIOLOGY OF AGING

11. PHYTOSOMAL ANTIAGING GEL –
Phytosomes are very recent introduction into herbal formulation as they are better absorbed and have higher
bioavailability. The term phyto means “plant” while “some” means cell like. These are advanced forms of herbal
formulation that contains bioactive phyto constituents of herbal extract surrounded by a lipid. Antiaging cream
reduces the wrinkles and blemishes on the skin. Gel is a semisolid preparation with two interpenetrating phases:
A gelling phase and a liquid.

12. ADVANTAGES OF PHYTOSOMES
1
2
4
3
5
Increases bioavailability
Optimum entrapment efficiency
Efficient nutrient safety
Hepatoprotective
Better stability

13. MATERIALS AND METHODS
MATERIALS - Tender coconut water was obtained from CDB lab, South Ernakulum. Aloe vera extract was
obtained from Elixir Extracts, Nellad Kochi.
METHODS –
i. Phytosomes are prepared by reacting the herbal extract in an aprotic solvent such as methylene chloride,
dioxane, and ethyl acetate with the phospholipid such as phosphatidylcholine, phosphatidyl ethanolamine,
or phosphatidylserine dissolved in the same solvent.

14. ii. After solubilization has completed, the complex compounds are isolated by removing the solvent
under vacuum, by freeze drying or by precipitation with non-solvents such as n-hexane.
iii. The obtained complexes are lipophilic in character and soluble in a polar and aprotic solvent, in
which the individual components of the complex are normally insoluble

15. FORMULATION OF PHYTOSOMES
Phosphatidylcholine
Dissolved in organic solvent containing drug
Hydration
Solution of phospholipid in organic solvent
Solvent evaporation
Formation of thin film
Formation of phytosomal suspension

16. SOLVENT EVAPORATION METHOD
Drug and Soya Lecithin
Concentrated mixture to 5-10 ml
Obtain the precipitate
Filter and collect
Phytosomes obtained
Refluxed with 20 ml of acetone at a temperature 50-60℃ for 24
hr

17. FORMULATION OF PHYTOSOMAL GEL
Carbopol 934 was dissolved in distilled water and stored overnight for
complete hydration
Active ingredients are dissolved in propylene glycol
Preservatives are added slowly with continuous stirring
Prepared phytosomes were incorporated into gel.

18. COMPOSITION
S.No
.
Ingredients F1 F2 F3 F4
1 Carbopol 934 1gram 1gram 1gram 1gram
2 Methyl Paraben 0.2ml 0.2ml 0.2ml 0.2ml
3 Propyl Paraben 0.1ml 0.1ml 0.1ml 0.1ml
4 Propylene Glycol
400
5ml 5ml 5ml 5ml
5 Triethanolamine 1.2ml 1.2ml 1.2ml 1.2ml
6 Tender Coconut
Water
10ml 10ml 10ml 10ml
7 Aloe vera extract 1ml 1ml - -
8 Grape seed extract 0.2gram - 0.2gram -
9 Vitamin E 0.1ml - - 0.1ml
10 Jojoba Oil 0.05ml 0.05ml 0.05ml 0.05ml

19. EVALUATION
 General Characterisation
I. In-vitro antioxidant studies
II. pH
III. Viscosity
IV. Spreadibility
V. Extrudability
VI. Vesicle Size and Zeta potential
VII. Stability Studies

20. I. In Vitro Antioxidant Studies
20
mcg/ml
40
mcg/ml
60
mcg/ml
80
mcg/ml
100
Mcg/ml
In Methanol
0.002% DPPH in methanol was used as free radical
Equal volume of different concentrations and
DPPH was mixed

21. Incubated at room temperature in dark for 30 min
Absorbance was measured at 517 nm
DPPH DPPHH
Yellow indicate scavenging efficiency
Percentage inhibition = [(Abs control – Abs sample) * 100 / (Abs control)]

22. II. pH of formulation –
1 % solution was made and pH was measured

23. III. Viscosity
Viscosity was determined by using Brookfield
viscometer at 100 rpm.

24. IV. Spreadalibity
Two glass slides of 20 cm × 20 cm were selected
A small amount of sample was sandwiched between the two glass slides.
A 100 gram of gel was placed uniformly between two slides
The weight was removed without any disturbance and upper slide was
removed slowly
Time taken in separation of two slides was observed using stop clock

25. CALCULATION –
S = m × L / T
Where,
S = Spreadability
m = Weight tied to the upper slide
L = Length of the glass
T = Time taken in seconds

26. V. Extrudability
The gel was filled in collapsible tube and sealed, weight recorded
Weight was done of extruded gel and percentage was calculated
500 gram weight was placed on tube and amount to gel extrude was
collected

27. VI. Vesicle Size & Zeta Potential
The particle size and zeta potential can be determined by dynamic light scattering (DLS) using a computerized
inspection system and photon correlation spectroscopy.

28. VII. Stability Studies A/c to ICH Guidelines for Cosmetics
8℃±1℃ 25℃±1℃ 45℃±1℃ 60℃±1℃
75% Relative Humidity
Observed for 60 days

29. RESULTS
I. In vitro Antioxidant Studies –

30. II. pH of phytosomal gel –
S.No. Formulations pH
1 F1 6.5
2 F2 6.4
3 F3 6.2
4 F4 6.3

31. III. Viscosity

32. IV. Spreadability

33. V. Extrudability

34. VII. Vesicle Size & Zeta Potential

36. VII. Stability Studies
Days Temperature Formulat-
ion
Parameters
pH Viscosity Spreadability Extrudability
0 8 F1 6.5 23108± 32.4±0.2 90.7±0.6
25 F2 6.4 27634± 35.7±0.2 90.9±0.9
45 F3 6.2 30192± 31.2±0.1 92.8±1.1
60 F4 6.3 35628± 35±0.8 92.9±1.3
20 8 F1 6.7 23106± 32.9±0.2 91.7±0.6
25 F2 6.4 27634± 35.8±0.2 90.2±0.9
45 F3 6.4 30195± 31.9±0.1 92.6±1.1
60 F4 6.5 35629± 35±5.8 93.6±1.3
40 8 F1 6.6 23108± 32.4±0.2 90.9±0.6
25 F2 6.4 27634± 35.7±0.2 90.8±0.9
45 F3 6.4 30195± 31.2±0.1 92.9±1.1
60 F4 6.5 35626± 35±0.8 92.1±1.3
60 8 F1 6.6 23109± 32.4±0.2 90.9±0.6
25 F2 6.4 27634± 35.7±0.2 90.9±0.9
45 F3 6.5 30195± 31.2±0.1 93.1±1.1
60 F4 6.5 35628± 35±0.8 93.5±1.3

37. CONCLUSION
 Antioxidants are the major ingredients present in antiaging cosmetics. The antioxidants
chosen for this study were tender coconut water, A. vera extract, grape seed extract, and
Vitamin E.
 F2 containing both tender coconut water and A. vera extract was chosen as the optimized
formula.
 Characterization studies of the optimized formulation such as vesicle size, size
distribution, and stability were performed, and it was identified that the prepared
antiaging phytosomal gel had all the parameters as optimum.
 Phytosomal gel having coconut water and aloe vera extract has better absorption and
bioavailability in skin.

38. REFERENCES
 https://www.researchgate.net/publication/323659309_Formulation_and_evaluation_of_antiaging_phyt
osomal_gel.
 https://link.springer.com/article/10.1208/s12249-015-0386-x
 http://repository-tnmgrmu.ac.in/6486/1/260107917_261510901.pdf
 https://rjptonline.org/HTMLPaper.aspx?Journal=Research%20Journal%20of%20Pharmacy%20and%2
0Technology;PID=2013-6-11-15.
 https://ijpsr.com/bft-article/phytosomes-a-novel-drug-delivery-for-herbal-extracts/?view=fulltext
 https://www.longdom.org/open-access/phytosomes-a-modernistic-approach-for-novel-herbal-drug-
delivery-enhancing-bioavailability-and-revealing-endless-frontier-ofphytop-44058.html
 https://www.longdom.org/open-access/phytosomes-a-modernistic-approach-for-novel-herbal-drug-
delivery-enhancing-bioavailability-and-revealing-endless-frontier-ofphytop-44058.html