cDNA library construction using mRNA which are derived from DNA. cDNA is formed from the reverse transcription of single stranded mRNA. cDNA contains only the exons, it donot not contains introns. The mRNA consists of poly A tail in which the tRNA and rRNA donot contains poly A tail. A short oligo nucleotide of Poly T is used to isolate mRNA seperately thereby single stranded mRNA is then converted into cDNA by using reverse transcriptase enzyme.

1. CONSTRUCTION OFCONSTRUCTION OF
cDNA LIBRARYcDNA LIBRARY
-SAMSUDEEN. SSAMSUDEEN. S
MSc. MicrobiologyMSc. Microbiology
AYYA NADAR JANAKI AMMAL COLLEGEAYYA NADAR JANAKI AMMAL COLLEGE
DEPARTMENT OF MICROBIOLOGYDEPARTMENT OF MICROBIOLOGY

4. WHAT IS cDNA ?WHAT IS cDNA ?

14. THANKTHANK
YOUYOU
AYYA NADAR JANAKI AMMAL COLLEGEAYYA NADAR JANAKI AMMAL COLLEGE
DEPARTMENT OF MICROBIOLOGYDEPARTMENT OF MICROBIOLOGY