Microbial Analysis on Some Coordination Compound of Metals with Ampicillin Original Research Article Journal of Chemistry and Materials Research Vol. 1 (2), 2014, 40–44 Pranay Guru*

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Journal of Chemistry and Materials Research
Vol. 1 (2), 2014, 40–44
JCMR
Journal of Chemistry and
Materials Research
ORICPublications
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Original Research
Microbial Analysis on Some Coordination Compound of Metals with
Ampicillin
Pranay Guru*
Department of Applied Sciences & Humanities, Bansal Institute of Research & Technology, Bhopal (M.P.) India
Received 22 Jun 2014; received in revised form 07 August 2014; accepted 09 August 2014
Abstract
The present paper deals with the microbial studies of the complex Co(II) and Zn(II) with antibiotic drug ampicillin, a formula
Co(C16H19N3O4S)2MoO4, 3H2O and Zn(C16H19N3O4S)2MoO4, 4H2O has been suggested on the basis of elemental analysis and molar
conductance for the newly synthesized complex. The microbial studies of synthesized complex were studied on pathogenic bacteria using gram
+ ve (Bacillus subtilis and Staphylococcus aureus) and gram – ve (Shigella flexneri, Salmonella typhosa, Escherichia coli) and some fungi
(Aspergillus flavus, Fusarium oxysporum, Chrysosporium pannicale, Alternaria solani, Candida albicans ).
Keywords: Bacillus subtilis; Staphylococcus aureus; Aspergillus flavus; Fusarium oxysporum; Chrysosporium pannicale; Alternaria solani;
Candida albicans.
1. Introduction
Co-ordination compounds exhibit different characteristic
properties which depend on the metal ion to which they are
bound. On the basis of nature of the metal as well as the type
of ligand, these metal complexes have extensive applications
in various fields of human interest [1–4]. Chelation or compl-
exation observes more potent antibacterial effect against some
microorganisms than the respective drug [5–10].
In continuation of the work being carried out in our
laboratory on the metal molybdate/ tungstate/ vanadate with
organic ligand [11–15], the present communication describes
microbial studies of Co(II) and Zn(II) with antibiotic drug
ampicillin (C16H19N3O4S) having Molybdate as anion.
* Corresponding author. Tel.: + 91-9826313501.
E-mail address: pranayguru@rediffmail.com (P. Guru).
All rights reserved. No part of contents of this paper may be reproduced or
transmitted in any form or by any means without the written permission of
ORIC Publications, www.oricpub.com.
2. Experimental and methods
Microbial studies of the synthesized complexes were perfo-
rmed at Department of Microbiology, Dr H.S. Gour University
Sagar (M.P.) and Govt. Veterinary college Jabalpur (M.P.)
using paper disc method Gupta et al [16], on the following
pathogenic bacteria using gram + ve (Bacillus subtilis and
Staphylococcus aureus) and gram – ve (Shigella flexneri,
Salmonella typhosa, Escherichia coli) and some fungi
(Aspergillus flavus, Fusarium oxysporum, Chrysosporium
pannicale, Alternaria solani, Candida albicans ).
3. Results and discussion
The synthesized complexes were screened for the antibact-
erial and antifungal activity using standard paper disc method
[17–20] against gram posative bacterial viz. Bacillus subtils
and Staphyloccus aureus and gram negative bacteria viz. Esch-
erichia coli and Salmonella typhosa and fungi Aspergillus
flavus, Alterneria solani, Candid albicans, Fusarium oxyspor-
um and Chrysosporium pannicle. In general, all the tested
complexes showed higher toxicity against bacterial and fungi
under study.

2. Pranay Guru / Journal of Chemistry and Materials Research 1 (2014) 40–44 41
Table 1 Complexes of different metals were marked has S1 and S2 as follows
S1- Co(C16H19N3O4S)2MoO43H2O
S2- Zn(C16H19N3O4S)2MoO44H2O
Table 2 Antibacterial activity of synthesized complexes.
S.
No
(a) B
a
(i)C
n
Stain of Bacteria/ Zone of Inhibition (mm*)
S1 S2
1
Shigella flexneri
0.01 M 14 17
0.5 M 22 25
0.1 M 26 30
2 Salmonella typhosa 0.01 M 13 16
0.5 M 18 22
0.1 M 24 29
3 Escherichia- coli 0.01M 13 17
0.5M 19 24
0.1M 25 29
4 Bacillus subtilis 0.01M 14 13
0.5 M 14 18
0.1 M 25 29
5 Staphylococcus aureus 0.01 M 14 18
0.5 M 19 23
0.1 M 24 29
Including diameter of filter-paper disc (6 mm)
S1 = Co(C16H19N3O4S)2MoO43H2O and S2 = Zn(C16H19N3O4S)2MoO44H2O
Table 3 Antifungal activity of synthesized complexes.
S.
No
(a) (i)Stain of Fungi/ Zone of Inhibition (mm*)
S1 S2
1 Aspergillus flavus 0.01 M 13 17
0.5 M 18 24
0.1 M 22 30
2 Candida albicans 0.01 M 12 15
0.5 M 17 22
0.1 M 24 29
3 Alternaria solani 0.01M 11 14
0.5M 16 26
0.1M 27 30
4 Fusarium oxysporum 0.01M 10 14
0.5 M 14 19
0.1 M 23 29
5 Chrysosporium pannicale 0.01 M 11 14
0.5 M 15 20
0.1 M 21 30
Including diameter of filter-paper disc (6mm)
S1 = Co(C16H19N3O4S)2MoO43H2O and S2 = Zn(C16H19N3O4S)2MoO44H2O

3. 42 Pranay Guru/ Journal of Chemistry and Materials Research 1 (2014) 40–44
a b c
d e
S1 = Co(C16H19N3O4S)2MoO43H2O S2 = Zn(C16H19N3O4S)2MoO44H2O
Fig. 1. Comparative antibacterial activity of complexes against: (a) Shigella flexneri, (b) Salmonella typhosa, (c) Escehrichia-coli, (d)
Bacillus subtilis and (e) Staphylococcus aureus.
3.1.Antibacterial activity
From the table 2 it is concluded that complex S2 has shown
maximum zone of inhibition against Shigella flexneri at the
concentration of 0.1 M even at the concentration of 0.01 M it
has shown good zone of inhibition in compression to other
tested complexes.
Against Salmonella typhosa good antibacterial activity was
observed against almost all the tested complexes. Complex S2
individually shown maximum zone of inhibition against this
organism. Against Escherichia–coli maximum inhibitory effect
were produced by complex S2.
Bacillus subtilis was found to be more susceptible against
complex S2 maximum zone of inhibition were recorded aga-
inst these tested complexes.
Complexes S2 found to be more active and shown higher
zone of inhibition against Staphylococcus aureus in compare-
ison to S1.
On comparing the anti-bacterial efficacy of these tested
complexes, it is concluded that though most of the complexes
reported satisfactory results for their antibacterial property but
complexes S2 in particular gave promising results. From the
above study it is observed that complex of Co(II) and Zn(II)
with titled ampicillin found to most active against the tested
microorganisms. It is found that all the tested complexes
exhibit good antibacterial activity at the concentration of 0.1
M and. it is interesting to note that inhibitory power of compl-
exes decrease with the increase of their concentration.
For the comparison of the antibacterial properties of these
tested complexes against bacteria Shigella flexneri, Salmonella
typhosa, Escherichia- coli, Bacillus subtilis, Staphylococcus
aureus the zone of inhibition have been graphically represe-
nted in Figs. 1a to e.
3.2.Anti fungal activity
Study of anti-fungal activity of complexes S1, S2 was
carried out against selected five fungi namely Aspergillus

4. Pranay Guru / Journal of Chemistry and Materials Research 1 (2014) 40–44 43
a b c
d e
S1 = Co(C16H19N3O4S)2MoO43H2O S2 = Zn(C16H19N3O4S)2MoO44H2O
Fig. 2. Comparative antibacterial activity of complexes against: (a) Aspergillus flavus, (b) Candida albicans, (c) Alternaria solani, (d)
Fusarium oxysporum and (e) Chrysporium pannicale.
flavus, Candida albicans, Alternaria solani, Fusarium oxysp-
orum and Chrysosporium pannicale. At varying concentration
of complexes 0.1M, 0.5 M and 0.01 M respectively and the
result are recorded in terms of zone of inhibition which also
includes the diameter of filter paper disc (6 mm).
From the table 3 it is observed that at the concentration of
0.1M of complex S2 shown maximum zone of inhibition was
recorded against Aspergillus flavus similarly good inhibitory
efficacy was also observed at the same concentration of
complexes S1 against Aspergillus flavus.
Against Candida albicans at the concentration of 0.1M
complex S2 have shown maximum activity but similarly,
considerable zone of inhibition were also recorded in case of
complexes S2. It is evident from the result even at the
concentration of 0.01 M all the complexes were found to be
active against Candida albicans.
Maximum zone of inhibition were recorded by all the
complexes at the concentration of 0.1M against Alternaria
solani. All the complexes at the concentration of 0.5 M have
also gave promising results.
The complex S2 at the concentration of 0.1 M produced
maximum zone of inhibition against Fusarium oxysporum..
Microorganism Chrysosporium pannicale was found susce-
ptible against all the complexes tested at their concentration of
0.1 and 0.5 Complex S2 was found to posse’s good antifungal
activity at 0.1 M concentration.
For the comparison of the antifungal properties of these
tested complexes against bacteria Aspergillus flavus, Candida
albicans, Alternaria solani, Fusarium oxysporum and Chryso-
sporium pannicale the zone of inhibition have been graphically
represented in Figs. 2a to e.
Antimicrobial properties of the original drug against select-
ed microorganism were also compared. It could be observed
that synthesized complex have shown promising result
compared to commercial original drug ampicillin [21–28].

5. 44 Pranay Guru/ Journal of Chemistry and Materials Research 1 (2014) 40–44
4. CONCLUSION
The results revealed that the synthesized complexes have
shown promising antibacterial activity. However, the zone of
inhibition of complex varies with organisms as well as metal
ions. Thus, it can be concluded that most of complexes possess
antimicrobial activities.
ACKNOWLEDGMENT
The author are thankful to Dr. A. K. Guru, Ex Director,
State Forensic Science Laboratory, Sagar (M.P.), India and
Head, Department of Chemistry & Head, Department of
Microbiology Dr. H. S. Gour University, Sagar (M.P.) for
providing necessary laboratory facilities and valuable
suggestions. The author is also thankful to HOD, Govt.
veterinary college Jabalpur (M.P.)
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