Electron microscope

2. ELECTRON MICROSCOPE
SHIBU MEHER
17MM01019
School of Minerals, Metallurgical and Materials Engineering

3. 3
Ernst RuskaMax Knoll

4. CONTENTS
4
1) Introduction
2) Types and working
3) Application
4) Recent advancement
5) Disadvantage
6) Conclusion
7) Reference

5. ELECTRON MICROSCOPE
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• Uses electron beam instead of light
• Has higher resolution than light
microscope (wavelength of electron is
100,000 times smaller than light)
• Uses electron optical lenses
• 10,000,000x magnification and 50pm
resolution

6. TYPES AND WORKING
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1) Transmission electron microscope
(TEM)
2) Scanning electron microscope (SEM)
3) Reflection electron microscope (REM)
4) Scanning transmission electron
microscope (STEM)

7. Applications
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• Circuit edit
• Defect analysis
• Electron tomography
• Virology, biochemistry
• Failure analysis and detection
• Protein localization
• Nanometrology
• Material qualification
• Chemical and Petrochemical
• Food science
• Forensics
• Mineral liberation analysis
(List continues to all those field
which is connected with analysis
of small particle )

8. Cool microscope technology revolutionize
biochemistry
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• Cryo-electron microscopy – higher
resolution structure
determination of biomolecules in
solution
• Freezing the mid-movement of
biomolecule and visualize
• 3D image of protein molecule
• Nobel Prize in Chemistry 2017
Protein complex A sensor Zika Virus

9. 9
TRPV6 channel in closed and open state

10. Naked molecules dancing in liquid
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• Movements of molecules are seen
inside graphene pockets without
staining
• Paves way to dynamics of life
molecule like protein, DNA etc

11. A glimpse inside atom
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• Imaging with electron microscope
requires complex calculation
• Looking inside atom to image
individual electron orbital
• Use of Energy Filtered Transmission
Electron Microscope

12. Electron tomography
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• Record of 3487 images in 3.5 seconds to
construct 3D electron tomogram by using
Transmission Electron Microscope (TEM)
• Previously – 10 to 60 minutes and ten fold
higher dose of electron
• Examining –
- Cell, bacteria and viruses
- Dynamic processes such as
chemical reaction
- Electron switching

13. 13
Electron tomography

14. DISADVANTAGES
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• Expensive to build and maintain
• For higher resolution – needs to be housed in
stabled building with magnetic field cancelling
system
• Specimen needs to observed in vacuum (Except
some exception)
• Non-conductive specimen needs to be coated
with conducting material (only for high voltage
EM)

15. CONCLUSION
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• Use of electron microscope has increase the area of our knowledge in
various field.
• Electron microscopy has contributed a lot to different field and its
contribution is increasing day by day.

16. REFERENCES
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• https://en.wikipedia.org/wiki/Electron_microscope
• Google image
• https://www.sciencedaily.com/releases/2017/12/171220131718.htm

17. THANK YOU
17