Briefly illustrating the connection between fertility mechanisms and the cannabioid system. Fertiprot is a leader in fertility solutions without needing IVF or any invasive interventions.

1. ©Dr. Ronen Shemesh
Endocannabinoid Signaling in
Reproduction and Fertility
The International Medical
Cannabis Conference
11-13 September, 2016
Dan Panorama Hotel, Tel Aviv, Israel

2. Localization of endocannabinoid system (ECS)
elements in the human sperm cell.
Cinzia Rapino, et al. Human Reproduction Update, Vol.20, No.4 pp. 501–516, 2014

3. Subcellular localization of CB1-R in human ejaculated spermatozoa
Region of the head
Regions of the neck
and mid-piece
Aquila Saveria , et al. THE ANATOMICAL RECORD 293:298–309 (2010)

4. Subcellular localization of CB1-R in human ejaculated spermatozoa
control sections
incubated without
the primary Ab
(b,c,f)
Transverse section
of the mid-piece
(d-e)
Aquila Saveria , et al. THE ANATOMICAL RECORD 293:298–309 (2010)
Sagital section of the
mid-piece and the
tail (a)

5. Δ9‐Tetrahydrocannabinol (Δ9‐THC) attenuates mouse sperm motility and male
fecundity
British Journal of Pharmacology
Volume 165, Issue 8, pages 2575-2583, 23 MAR 2012 DOI: 10.1111/j.1476-5381.2011.01506.x
http://onlinelibrary.wiley.com/doi/10.1111/j.1476-5381.2011.01506.x/full#f2
Basal and bicarbonate-stimulated motility is inhibited by Δ9-
THC. (A) Treatment with 1 µM Δ9-THC or 10 µM Δ9-THC for
15 min progressively reduced the percentage of motile
sperm. (n= 111–138 cells). At least eight cells were
examined from each animal in two to three independent
experiments. #P < 0.05 (untreated vs. 1 µM THC), *P < 0.05
(untreated vs. 10 µM THC).

6. Effect of Meth-AEA and THC on sperm motility.
A.A. Amoako, et al. Human Reproduction, Vol.28, No.8 pp. 2058–2066, 2013
Motile fractions of
human spermatozoa
isolated by direct swim-
up were incubated with
Meth-AEA (1 mM) or
without (0.2% DMSO in
BWW medium) for up
to 90 min (A).
Removal of Meth-
AEA reverses the
inhibitory effect on
sperm motility (B).

7. Effect of MET-F-AEA on sperm viability, Acrosin activity and .
Aquila Saveria , et al. THE ANATOMICAL RECORD 293:298–309 (2010)

8. Effects of meth-AEA on human sperm motility and viability.
A.A. Amoako, et al. Human Reproduction, Vol.28, No.8 pp. 2058–2066, 2013
AM251 reverses
the AEA effect
on Motility and
Viability

9. CB1 receptor blockade by rimonabant influences sperm parameters
Aquila Saveria , et al. British Journal of Pharmacology (2010), 159, 831–841
Rimonabant positively affects sperm motility and viability. Rimonabant positively affects sperm Acrosin activity.
Rim = Rimonabant
URB = FAAH inhibitor URB597

10. LIF and AEA levels are essential for successful pregnancy.
Cinzia Rapino, et al. Human Reproduction Update, Vol.20, No.4 pp. 501–516, 2014

11. THC and control of hormonal cycle
Brents LK. Yale J Biol Med. 2016 Jun; 89(2): 175–191.

12. Cannabinoids in Blastocyst migration and implantation
Brents LK. Yale J Biol Med. 2016 Jun; 89(2): 175–191.
implantation can only
occur in areas that express
low CB1R and AEA.
Adequate levels of CB1R expression are
required in the Fallopian tube to allow
passage of the embryo into the uterus and
prevent ectopic implantation.
Exposure to high concentrations of Δ9-
THC or anandamide will arrest the
development of the blastocyst

13. Cannabinoid signaling impacts various pregnancy events.
Life Sci. 2014 Feb 27; 97(1): 72–77.

14. AEA levels through the menstrual cycle and gestation.
Cinzia Rapino, et al. Human Reproduction Update, Vol.20, No.4 pp. 501–516, 2014
pre-eclampsia
patients
ectopic or non-
viable pregnancies