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AETCOM in Microbiology
18-01-2023 1
Indian Medical Graduate (IMG)
• Healthcare needs of community keep changing - medical education
system continuously evolves - adapt to changing needs
• To make existing traditional medical education system more relevant-
IMG, MCI- introduced Competency-Based Medical Education (CBME)
from the year 2019- the first major revision to the medical curriculum
since 1997 after 20 long years
18-01-2023 2
Indian Medical Graduate (IMG)
• Overall goal of the undergraduate medical education program-
by MCI, in the revised Graduate Medical Education Regulations-
(2019) create an “Indian Medical Graduate (IMG)”- knowledge,
skills, attitudes, values and responsiveness- function
appropriately and effectively- physician of first contact-
community while being globally relevant
18-01-2023 3
Roles of IMG
1. Clinician who understands, provides preventive care with
compassion
2. Leader & member of the healthcare team & system- capabilities to
collect, analyze, synthesize & communicate health data appropriately
3. Communicator with patients, families, colleagues and community
4. Lifelong learner- continuous improvement of skills and knowledge
5. Professional- committed to excellence, is ethical, responsive and
accountable to patients, community and profession
18-01-2023 4
AETCOM
• Refers to the soft skills ‘Attitude, Ethics & Communication,’
that an IMG needs to learn along with the knowledge and
clinical skills to provide holistic healthcare
• Assessment system in place- ensure that the learning about
AETCOM has actually taken place
18-01-2023 5
AETCOM
Essential components of AETCOM are as follows:
• Attitude
• Ethics: has the 4 pillars-
 Patient autonomy
 Beneficence
 Non-maleficence
 Social justice
• Communication
18-01-2023 6
Need for AETCOM Competencies
• Proficiency in AETCOM competencies- essential in carrying out
routine healthcare activities- doctor-patient interactions, practicing
informed decision making, breaking bad news, communication and
documentation
• Lack of effective communication- medical errors, mistakes in
diagnosis, inaccurate treatment, compromised patient safety, patient
noncompliance- stressful legal and sociocultural issues
18-01-2023 7
AETCOM Learning in Current vs
Previous Curriculum
• AETOM in earlier MBBS curriculum: Before CBME was
introduced, medical education focused- gaining knowledge and
clinical skills
• AETCOM competencies were neither formally taught nor
assessed
• Some learning used to happen by observing seniors (role
modelling) or with self-experience
18-01-2023 8
AETCOM Learning in Current vs
Previous Curriculum
• AETCOM in current curriculum: aim at acquisition of minimum
essential skills- communicating effectively & sympathetically with
patients
Longitudinally spread: AETCOM competencies relevant to each
subject- defined in CBME- taught longitudinally- phases of MBBS
Increasing complexity: topics/competencies are interlinked
18-01-2023 9
T-L Methods for AETCOM Competencies
• Teaching-Learning sessions: students are provided with
opportunities to learn basic essential background
knowledge, opportunities to learn by experiencing (mostly
simulated) & reflect on experiences
• Innovative teaching-learning (TL) methods: more engaging &
effective
18-01-2023 10
Problem-based Learning (PBL)
• Suggested by MCI as main TL method for AETCOM
• Helps students explore various facets of “real life issues” that will
confront them in their careers, develop problem solving skills
• Case discussions promote collaborative learning and team work,
reflection and self-directed learning
18-01-2023 11
Assessment of AETCOM Competencies
• Formative Assessment: done during & along with day-to-day T-L
sessions- to provide feedback to students and help them improve
Based on student participation in small group discussions,
performance in assignments or internal assessment tests
• Summative Assessment: qualifying examination which decides
pass or fail status
Questions on AETCOM competencies in theory & practical exams
conducted at end of each professional year
Student- maintain logbook as record of his performance
18-01-2023 12
Suggested AETCOM Topics in
Microbiology
Following competencies are specified in Microbiology subject:
1. Demonstration of confidentiality pertaining to patient
identity on laboratory results
2. Demonstration of respect for patient samples sent to
laboratory for performance of laboratory tests
18-01-2023 13
Competency-1:
Demonstrate Confidentiality
Pertaining
to Patient Identity on Laboratory
Results
18-01-2023 14
Sample Case Scenarios
Case scenario 1 (Disclosing HIV result):
• A lady aged 20 years admitted for fever & breathlessness jumps from
4th floor of hospital & dies- recently diagnosed to be HIV reactive
(one week ago) and “Retro positive” labels on her case file and bed.
Family members- know about her HIV status.
They became stressed & shouted at patient. Except her mother, all
other family stopped visiting her
18-01-2023 15
Sample Case Scenarios
Case scenario 2 (Not disclosing HIV result):
• A 33-year man- admitted to emergency ward with multiple
limb fractures. His vitals stabilized, conscious & oriented.
Emergency surgery- planned. His relative comes to lab to
collect investigation reports, given all reports except HIV
results. He is specifically asking if lab is not issuing only HIV
report because it is reactive. Technician gets call from ward
asking for HIV report immediately over phone, as t patient is
being shifted to operating room
18-01-2023 16
Sample Case Scenarios
Case scenario 3 (Patient refuses to provide samples):
• A nurse caring for admitted patient gets needle stick injury
with syringe used for the patient. The patient’s HIV and HBV
infection status unknown. To decide for PEP, nurse wants to
get patient tested. Patient asks the nurse not to worry,
refuses saying he has no such infection, hence no need to
test & has financial constrain for performing the tests. The
nurse is anxious. Nurse decides to use the patient’s blood
sample collected for some other tests for testing for HIV and
HBsAg and bear the cost by herself
18-01-2023 17
Sample Case Scenarios
Case scenario 4 (Social stigma in COVID-19):
• A patient with influenza like illness (ILI) has come to the
screening OPD. He had exposure to a confirmed COVID-19 case
five days back. He wants to get tested and treated, as he is
anxious. However, he does not want to be quarantined or
discriminated, and has a fear of social stigma and losing the job.
He is also the only caretaker of old parents at home
18-01-2023 18
Sample Case Scenarios
Case scenario 5 (Occupational exposure):
• A medical intern comes to the infection control division ,
history of NSI 1 hour back. The source patient’s blood sample
collected and tested for HIV, HBV and HCV. Intern is waiting
in the reception to know about the test result of source. Test
result is reactive for HIV but negative for hepatitis B and C.
The infection control officer discloses the source result to the
intern and provides appropriate counselling and PEP. He also
forwards the source result to ICTC. The ICTC calls the source
patient on next day and informs about the test result after
providing counselling
18-01-2023 19
Essential Background Knowledge
1. Principles of medical ethics
2. Medicolegal aspects of confidentiality
3. Confidentiality & privileged communication related to lab.
results
4. Modes of transmission & diagnostic approach for HIV or
COVID-19
5. Sociocultural issues related to sensitive infections like HIV,
COVID-19 or needle stick injury
6. PEP for NSI (needle stick injury) when source turns out to be
positive for HIV or HBV
18-01-2023 20
Specific Learning Objectives
1. Discuss rights and responsibilities of patients (or healthcare
worker in case scenario 5)
2. Discuss rights and responsibilities of laboratory with respect
to confidentiality of laboratory results
3. Analyze ethical issues involved in confidentiality pertaining
to patient identity
18-01-2023 21
Specific Learning Objectives
4. Describe medicolegal consequences of breach in
confidentiality
5. Demonstration of sympathy when breaking through of
result to healthcare workers, providing counselling and
maintaining confidentiality pertaining to occupational hazards
such as needle stick injury (in case scenario 5)
18-01-2023 22
Teaching-Learning Method
1. Introductory session: introduction of paper case in small group discussion,
identification of various aspects involved, framing learning objectives and deciding
assignments along with learning resources
2. SDL: self-directed learning by students
3. Anchoring learning sessions: involves one or more of following depending upon
case scenario:
Interaction with laboratory technician and counsellor of ICTC
Interaction with Microbiology laboratory technician involved in HIV/COVID-19
testing and report dispatch
Interaction with infection control officer involved in management of NSI
18-01-2023 23
Teaching-Learning Method
4. Concluding session: small group discussion of various possible
approaches for case, their pros and cons, and justification for best
approach selected by each student. However, it may be possible that
there may not be single best approach
5. Writing narratives by the students about their learning experiences
18-01-2023 24
Assessment
• Formative: participation in group discussion, assignments, reflection
writing, MCQs to assess relevant background knowledge, OSPE
• Summative (Theory): short notes and short answer questions
• Summative (Practical): OSPE with simulated patient–HIV pre-test/
post-test counselling, counselling following NSI, informing positive
COVID-19 report to patient and informing NSI test result to the HCW
18-01-2023 25
Key Learning Points
1. Confidentiality
2. Informed consent
3. Counselling
4. Privileged communication
5. Method followed in ICTC for HIV testing
6. Method followed for other STDs (Syphilis, Gonorrhea)
7. Method followed for COVID-19
18-01-2023 26
Competency-2
Demonstration of Respect
for Patient Samples
18-01-2023 27
Sample Case Scenarios
Case scenario 1 (Rejection due to improper transport):
• Sequestrum from chronic osteomyelitis case was debrided and sent
for culture and sensitivity.
• The sample was rejected by the laboratory mentioning that it was
received in formalin, hence unsuitable for culture.
• There is no more sample available for culture now
18-01-2023 28
Sample Case Scenarios
Case scenario 2 (Specimen did not reach laboratory):
• A critically ill 5-year-old child’s CSF report is awaited for 3 days. On
enquiry laboratory says it did not receive the sample. On further
probing it was found that the nursing staff had kept the small bottle
with the sample in his pocket and mistakenly taken it outside the
hospital and had dropped it somewhere, and did not submit it to the
laboratory for testing. Now, the baby needs to undergo lumbar
puncture again, results may not be the same as antibiotics are given
and need to wait for some more days for the culture report
18-01-2023 29
Sample Case Scenarios
Case scenario 3 (Misguided report due to inadequate information in
requisition form):
• Urologist calls laboratory to discuss about “Insignificant bacteriuria”
culture report of a pyelonephritis patient. He says it was a
percutaneous nephrostomy sample and asks for the organism and
antimicrobial sensitivity. Microbiologist says it was written as urine
sample on the request form, some gram-negative bacillus had grown
and the count was less than 10,000 CFU/mL, so it was thought to be a
periurethral commensal and the isolate was discarded, and hence
further testing cannot be done
18-01-2023 30
Sample Case Scenarios
Case scenario 4 (Specimen kept at wrong place):
• Junior resident gets angry and yells at the patient on noticing a stool
sample kept on the bedside. The patient’s attendant tries to explain
that the container is covered in a plastic cover and all these days the
junior resident herself used to keep collected blood and swab
samples in that very same place, and he was not informed that stool
sample was not to be kept on the side table
18-01-2023 31
Sample Case Scenarios
Case scenario 5 (Rejection due to improper collection):
• A suspected pulmonary tuberculosis patient, who would travel 30 km
from his village to the private hospital with the attached laboratory in
the city, had submitted spot sputum sample the previous day and an
early morning sample today for acid-fast staining. Reports of both the
samples mentioned “many epithelial cells suggestive of excessive
salivary contamination. Repeat with the proper sample”. Blood
culture was also collected from the patient by the clinical team, the
result of which came as contaminated blood culture specimen with
patient’s skin flora
18-01-2023 32
Sample Case Scenarios
Case scenario 6 (Sample collected for culture and sensitivity in
unsterile container):
• Paired blood specimen (5 mL each) was sent to the laboratory in two
vacutainers for blood culture. The laboratory rejected the specimen.
The patient screams that he cannot allow to draw another set of
blood specimen for investigation
18-01-2023 33
Sample Case Scenarios
Case scenario 7 (Rejection due to lack of patient informations)
• Microbiology lab rejects a bunch of specimens because one or the
other relevant informations were missing in those specimens -
patient’s name, age or gender, ward, hospital number, sample type,
clinical diagnosis, or treatment history. Clinical team screams at the
lab that they could have called the ward or the patient’s attendant
and verified the details instead of rejecting
18-01-2023 34
Sample Case Scenarios
Case scenario 8 (Rejection due to mismatch of name)
• Microbiology lab rejects a blood culture specimen collected in
BacT/ALERT bottle because the patient’s name written on the bottle
did not match with the requisition form. Clinical team asks the patient
to pay again for a repeat blood culture investigation. The patient
complains that he cannot afford the price for another test and neither
he can give consent to draw another specimen
18-01-2023 35
Sample Case Scenarios
Case scenario 9 (Prioritizing a sample requiring immediate processing
and reporting over the others)
• In the midnight, the Microbiology laboratory receives three
specimens (urine, sputum, CSF) from a patient for culture. The
technician was already processing a huge load of investigations,
therefore he informed the clinical team that these specimens can only
be processed on the next day
18-01-2023 36
Essential Background Knowledge
1. Appropriate sample for the test planned: sample type,
amount, collection procedure, preservative if any, container
type used and its transportation and storage
2. Appropriate labelling for correct sample identification
3. Accompanying clinical information for correlation
18-01-2023 37
Essential Background Knowledge
4. Possible medicolegal issues following incomplete/incorrect sample
identification
5. Sociocultural issues following incomplete/incorrect sample
identification, relevant clinical information for correlation, improper
storage/transportation
6. Ethical issues following incomplete/incorrect sample identification,
relevant clinical information for correlation, improper
storage/transportation
18-01-2023 38
Specific Learning Objectives
1. Choose appropriate container for sample collection
2. Demonstrate appropriate procedure for temporary storage
& transportation of clinical sample
3. Discuss information that shall be written in request form &
sample container, completely and legibly
18-01-2023 39
Specific Learning Objectives
4. Discuss judicious application of sample rejection criteria in best
interest of patient care
5. Discuss importance of prioritizing specimen as relevant to
clinical situation
6. Discuss medical, ethical & socio-economical considerations of
errors in sample collection and submission process
18-01-2023 40
Teaching-Learning Methods
1. Introduction of scenarios with help of paper case/role
plays/videos
2. Small group discussion: identification of clinical, medicolegal,
sociocultural & ethical issues involved
3. Writing learning objectives
4. Writing narratives by students about their learning experiences
18-01-2023 41
Teaching-Learning Methods
5. Anchoring lecture and demonstration of appropriate procedure of
sample collection, transportation and reception at laboratory
Discussion with nursing staff, phlebotomist, laboratory technicians to
gather first-hand information
6. Closing session with small group discussion
7. Writing narratives by students about their learning experiences
18-01-2023 42
Assessment
• Formative
• Summative (Theory)
• Summative (Practical): OSPE can be conducted covering:
Sample collection with care and empathy, instructing
patients on appropriate sample collection (e.g. urine,
sputum, blood culture)
Labelling sample containers and filling request form for
the clinical scenario provided
18-01-2023 43
Key Learning Points
Specimen rejection criteria: Reasons for sample rejection
include:
Improperly labelled or unlabeled sample
Incomplete specimen-related or clinical information on
sample
Sub-optimal sample
Duplicate microbiology samples received on same day
Sample delayed in transit more than accepted limit
18-01-2023 44
Key Learning Points
• Specimen collection
• Prioritizing specimen for processing: certain precious specimens -
CSF and sterile body fluids, ocular specimens, tissue specimens,
suprapubic aspirate and bone specimen- processed immediately
as soon as received, not more than 15 min delay
Blood culture bottles should be immediately incubated upon
receipt
18-01-2023 45

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AETCOM.pptx

  • 2. Indian Medical Graduate (IMG) • Healthcare needs of community keep changing - medical education system continuously evolves - adapt to changing needs • To make existing traditional medical education system more relevant- IMG, MCI- introduced Competency-Based Medical Education (CBME) from the year 2019- the first major revision to the medical curriculum since 1997 after 20 long years 18-01-2023 2
  • 3. Indian Medical Graduate (IMG) • Overall goal of the undergraduate medical education program- by MCI, in the revised Graduate Medical Education Regulations- (2019) create an “Indian Medical Graduate (IMG)”- knowledge, skills, attitudes, values and responsiveness- function appropriately and effectively- physician of first contact- community while being globally relevant 18-01-2023 3
  • 4. Roles of IMG 1. Clinician who understands, provides preventive care with compassion 2. Leader & member of the healthcare team & system- capabilities to collect, analyze, synthesize & communicate health data appropriately 3. Communicator with patients, families, colleagues and community 4. Lifelong learner- continuous improvement of skills and knowledge 5. Professional- committed to excellence, is ethical, responsive and accountable to patients, community and profession 18-01-2023 4
  • 5. AETCOM • Refers to the soft skills ‘Attitude, Ethics & Communication,’ that an IMG needs to learn along with the knowledge and clinical skills to provide holistic healthcare • Assessment system in place- ensure that the learning about AETCOM has actually taken place 18-01-2023 5
  • 6. AETCOM Essential components of AETCOM are as follows: • Attitude • Ethics: has the 4 pillars-  Patient autonomy  Beneficence  Non-maleficence  Social justice • Communication 18-01-2023 6
  • 7. Need for AETCOM Competencies • Proficiency in AETCOM competencies- essential in carrying out routine healthcare activities- doctor-patient interactions, practicing informed decision making, breaking bad news, communication and documentation • Lack of effective communication- medical errors, mistakes in diagnosis, inaccurate treatment, compromised patient safety, patient noncompliance- stressful legal and sociocultural issues 18-01-2023 7
  • 8. AETCOM Learning in Current vs Previous Curriculum • AETOM in earlier MBBS curriculum: Before CBME was introduced, medical education focused- gaining knowledge and clinical skills • AETCOM competencies were neither formally taught nor assessed • Some learning used to happen by observing seniors (role modelling) or with self-experience 18-01-2023 8
  • 9. AETCOM Learning in Current vs Previous Curriculum • AETCOM in current curriculum: aim at acquisition of minimum essential skills- communicating effectively & sympathetically with patients Longitudinally spread: AETCOM competencies relevant to each subject- defined in CBME- taught longitudinally- phases of MBBS Increasing complexity: topics/competencies are interlinked 18-01-2023 9
  • 10. T-L Methods for AETCOM Competencies • Teaching-Learning sessions: students are provided with opportunities to learn basic essential background knowledge, opportunities to learn by experiencing (mostly simulated) & reflect on experiences • Innovative teaching-learning (TL) methods: more engaging & effective 18-01-2023 10
  • 11. Problem-based Learning (PBL) • Suggested by MCI as main TL method for AETCOM • Helps students explore various facets of “real life issues” that will confront them in their careers, develop problem solving skills • Case discussions promote collaborative learning and team work, reflection and self-directed learning 18-01-2023 11
  • 12. Assessment of AETCOM Competencies • Formative Assessment: done during & along with day-to-day T-L sessions- to provide feedback to students and help them improve Based on student participation in small group discussions, performance in assignments or internal assessment tests • Summative Assessment: qualifying examination which decides pass or fail status Questions on AETCOM competencies in theory & practical exams conducted at end of each professional year Student- maintain logbook as record of his performance 18-01-2023 12
  • 13. Suggested AETCOM Topics in Microbiology Following competencies are specified in Microbiology subject: 1. Demonstration of confidentiality pertaining to patient identity on laboratory results 2. Demonstration of respect for patient samples sent to laboratory for performance of laboratory tests 18-01-2023 13
  • 14. Competency-1: Demonstrate Confidentiality Pertaining to Patient Identity on Laboratory Results 18-01-2023 14
  • 15. Sample Case Scenarios Case scenario 1 (Disclosing HIV result): • A lady aged 20 years admitted for fever & breathlessness jumps from 4th floor of hospital & dies- recently diagnosed to be HIV reactive (one week ago) and “Retro positive” labels on her case file and bed. Family members- know about her HIV status. They became stressed & shouted at patient. Except her mother, all other family stopped visiting her 18-01-2023 15
  • 16. Sample Case Scenarios Case scenario 2 (Not disclosing HIV result): • A 33-year man- admitted to emergency ward with multiple limb fractures. His vitals stabilized, conscious & oriented. Emergency surgery- planned. His relative comes to lab to collect investigation reports, given all reports except HIV results. He is specifically asking if lab is not issuing only HIV report because it is reactive. Technician gets call from ward asking for HIV report immediately over phone, as t patient is being shifted to operating room 18-01-2023 16
  • 17. Sample Case Scenarios Case scenario 3 (Patient refuses to provide samples): • A nurse caring for admitted patient gets needle stick injury with syringe used for the patient. The patient’s HIV and HBV infection status unknown. To decide for PEP, nurse wants to get patient tested. Patient asks the nurse not to worry, refuses saying he has no such infection, hence no need to test & has financial constrain for performing the tests. The nurse is anxious. Nurse decides to use the patient’s blood sample collected for some other tests for testing for HIV and HBsAg and bear the cost by herself 18-01-2023 17
  • 18. Sample Case Scenarios Case scenario 4 (Social stigma in COVID-19): • A patient with influenza like illness (ILI) has come to the screening OPD. He had exposure to a confirmed COVID-19 case five days back. He wants to get tested and treated, as he is anxious. However, he does not want to be quarantined or discriminated, and has a fear of social stigma and losing the job. He is also the only caretaker of old parents at home 18-01-2023 18
  • 19. Sample Case Scenarios Case scenario 5 (Occupational exposure): • A medical intern comes to the infection control division , history of NSI 1 hour back. The source patient’s blood sample collected and tested for HIV, HBV and HCV. Intern is waiting in the reception to know about the test result of source. Test result is reactive for HIV but negative for hepatitis B and C. The infection control officer discloses the source result to the intern and provides appropriate counselling and PEP. He also forwards the source result to ICTC. The ICTC calls the source patient on next day and informs about the test result after providing counselling 18-01-2023 19
  • 20. Essential Background Knowledge 1. Principles of medical ethics 2. Medicolegal aspects of confidentiality 3. Confidentiality & privileged communication related to lab. results 4. Modes of transmission & diagnostic approach for HIV or COVID-19 5. Sociocultural issues related to sensitive infections like HIV, COVID-19 or needle stick injury 6. PEP for NSI (needle stick injury) when source turns out to be positive for HIV or HBV 18-01-2023 20
  • 21. Specific Learning Objectives 1. Discuss rights and responsibilities of patients (or healthcare worker in case scenario 5) 2. Discuss rights and responsibilities of laboratory with respect to confidentiality of laboratory results 3. Analyze ethical issues involved in confidentiality pertaining to patient identity 18-01-2023 21
  • 22. Specific Learning Objectives 4. Describe medicolegal consequences of breach in confidentiality 5. Demonstration of sympathy when breaking through of result to healthcare workers, providing counselling and maintaining confidentiality pertaining to occupational hazards such as needle stick injury (in case scenario 5) 18-01-2023 22
  • 23. Teaching-Learning Method 1. Introductory session: introduction of paper case in small group discussion, identification of various aspects involved, framing learning objectives and deciding assignments along with learning resources 2. SDL: self-directed learning by students 3. Anchoring learning sessions: involves one or more of following depending upon case scenario: Interaction with laboratory technician and counsellor of ICTC Interaction with Microbiology laboratory technician involved in HIV/COVID-19 testing and report dispatch Interaction with infection control officer involved in management of NSI 18-01-2023 23
  • 24. Teaching-Learning Method 4. Concluding session: small group discussion of various possible approaches for case, their pros and cons, and justification for best approach selected by each student. However, it may be possible that there may not be single best approach 5. Writing narratives by the students about their learning experiences 18-01-2023 24
  • 25. Assessment • Formative: participation in group discussion, assignments, reflection writing, MCQs to assess relevant background knowledge, OSPE • Summative (Theory): short notes and short answer questions • Summative (Practical): OSPE with simulated patient–HIV pre-test/ post-test counselling, counselling following NSI, informing positive COVID-19 report to patient and informing NSI test result to the HCW 18-01-2023 25
  • 26. Key Learning Points 1. Confidentiality 2. Informed consent 3. Counselling 4. Privileged communication 5. Method followed in ICTC for HIV testing 6. Method followed for other STDs (Syphilis, Gonorrhea) 7. Method followed for COVID-19 18-01-2023 26
  • 27. Competency-2 Demonstration of Respect for Patient Samples 18-01-2023 27
  • 28. Sample Case Scenarios Case scenario 1 (Rejection due to improper transport): • Sequestrum from chronic osteomyelitis case was debrided and sent for culture and sensitivity. • The sample was rejected by the laboratory mentioning that it was received in formalin, hence unsuitable for culture. • There is no more sample available for culture now 18-01-2023 28
  • 29. Sample Case Scenarios Case scenario 2 (Specimen did not reach laboratory): • A critically ill 5-year-old child’s CSF report is awaited for 3 days. On enquiry laboratory says it did not receive the sample. On further probing it was found that the nursing staff had kept the small bottle with the sample in his pocket and mistakenly taken it outside the hospital and had dropped it somewhere, and did not submit it to the laboratory for testing. Now, the baby needs to undergo lumbar puncture again, results may not be the same as antibiotics are given and need to wait for some more days for the culture report 18-01-2023 29
  • 30. Sample Case Scenarios Case scenario 3 (Misguided report due to inadequate information in requisition form): • Urologist calls laboratory to discuss about “Insignificant bacteriuria” culture report of a pyelonephritis patient. He says it was a percutaneous nephrostomy sample and asks for the organism and antimicrobial sensitivity. Microbiologist says it was written as urine sample on the request form, some gram-negative bacillus had grown and the count was less than 10,000 CFU/mL, so it was thought to be a periurethral commensal and the isolate was discarded, and hence further testing cannot be done 18-01-2023 30
  • 31. Sample Case Scenarios Case scenario 4 (Specimen kept at wrong place): • Junior resident gets angry and yells at the patient on noticing a stool sample kept on the bedside. The patient’s attendant tries to explain that the container is covered in a plastic cover and all these days the junior resident herself used to keep collected blood and swab samples in that very same place, and he was not informed that stool sample was not to be kept on the side table 18-01-2023 31
  • 32. Sample Case Scenarios Case scenario 5 (Rejection due to improper collection): • A suspected pulmonary tuberculosis patient, who would travel 30 km from his village to the private hospital with the attached laboratory in the city, had submitted spot sputum sample the previous day and an early morning sample today for acid-fast staining. Reports of both the samples mentioned “many epithelial cells suggestive of excessive salivary contamination. Repeat with the proper sample”. Blood culture was also collected from the patient by the clinical team, the result of which came as contaminated blood culture specimen with patient’s skin flora 18-01-2023 32
  • 33. Sample Case Scenarios Case scenario 6 (Sample collected for culture and sensitivity in unsterile container): • Paired blood specimen (5 mL each) was sent to the laboratory in two vacutainers for blood culture. The laboratory rejected the specimen. The patient screams that he cannot allow to draw another set of blood specimen for investigation 18-01-2023 33
  • 34. Sample Case Scenarios Case scenario 7 (Rejection due to lack of patient informations) • Microbiology lab rejects a bunch of specimens because one or the other relevant informations were missing in those specimens - patient’s name, age or gender, ward, hospital number, sample type, clinical diagnosis, or treatment history. Clinical team screams at the lab that they could have called the ward or the patient’s attendant and verified the details instead of rejecting 18-01-2023 34
  • 35. Sample Case Scenarios Case scenario 8 (Rejection due to mismatch of name) • Microbiology lab rejects a blood culture specimen collected in BacT/ALERT bottle because the patient’s name written on the bottle did not match with the requisition form. Clinical team asks the patient to pay again for a repeat blood culture investigation. The patient complains that he cannot afford the price for another test and neither he can give consent to draw another specimen 18-01-2023 35
  • 36. Sample Case Scenarios Case scenario 9 (Prioritizing a sample requiring immediate processing and reporting over the others) • In the midnight, the Microbiology laboratory receives three specimens (urine, sputum, CSF) from a patient for culture. The technician was already processing a huge load of investigations, therefore he informed the clinical team that these specimens can only be processed on the next day 18-01-2023 36
  • 37. Essential Background Knowledge 1. Appropriate sample for the test planned: sample type, amount, collection procedure, preservative if any, container type used and its transportation and storage 2. Appropriate labelling for correct sample identification 3. Accompanying clinical information for correlation 18-01-2023 37
  • 38. Essential Background Knowledge 4. Possible medicolegal issues following incomplete/incorrect sample identification 5. Sociocultural issues following incomplete/incorrect sample identification, relevant clinical information for correlation, improper storage/transportation 6. Ethical issues following incomplete/incorrect sample identification, relevant clinical information for correlation, improper storage/transportation 18-01-2023 38
  • 39. Specific Learning Objectives 1. Choose appropriate container for sample collection 2. Demonstrate appropriate procedure for temporary storage & transportation of clinical sample 3. Discuss information that shall be written in request form & sample container, completely and legibly 18-01-2023 39
  • 40. Specific Learning Objectives 4. Discuss judicious application of sample rejection criteria in best interest of patient care 5. Discuss importance of prioritizing specimen as relevant to clinical situation 6. Discuss medical, ethical & socio-economical considerations of errors in sample collection and submission process 18-01-2023 40
  • 41. Teaching-Learning Methods 1. Introduction of scenarios with help of paper case/role plays/videos 2. Small group discussion: identification of clinical, medicolegal, sociocultural & ethical issues involved 3. Writing learning objectives 4. Writing narratives by students about their learning experiences 18-01-2023 41
  • 42. Teaching-Learning Methods 5. Anchoring lecture and demonstration of appropriate procedure of sample collection, transportation and reception at laboratory Discussion with nursing staff, phlebotomist, laboratory technicians to gather first-hand information 6. Closing session with small group discussion 7. Writing narratives by students about their learning experiences 18-01-2023 42
  • 43. Assessment • Formative • Summative (Theory) • Summative (Practical): OSPE can be conducted covering: Sample collection with care and empathy, instructing patients on appropriate sample collection (e.g. urine, sputum, blood culture) Labelling sample containers and filling request form for the clinical scenario provided 18-01-2023 43
  • 44. Key Learning Points Specimen rejection criteria: Reasons for sample rejection include: Improperly labelled or unlabeled sample Incomplete specimen-related or clinical information on sample Sub-optimal sample Duplicate microbiology samples received on same day Sample delayed in transit more than accepted limit 18-01-2023 44
  • 45. Key Learning Points • Specimen collection • Prioritizing specimen for processing: certain precious specimens - CSF and sterile body fluids, ocular specimens, tissue specimens, suprapubic aspirate and bone specimen- processed immediately as soon as received, not more than 15 min delay Blood culture bottles should be immediately incubated upon receipt 18-01-2023 45