1. Career Objective I strongly desire to acquire knowledge and skills to build a successful
career in life science.
Experiences Research Assistant
I2IT-IGNOU CoE for Advanced Education and Research, Pune
- Supervise students in area of Developmental Biology, Cancer
Biology and Stem Cell Biology.
- Worked on research projects.
Assistant Professor
MGM’s Institute of Biosciences, Aurangabad
- Conduct lectures and practical sessions for graduates and post
graduate students in Biomolecules, Cell Biology, Molecular
Genetics, General Virology.
Expertise (Research Interest) - Cancer Biology
- Stem cell and Developmental Biology
- Recombinant DNA Technology
Technical Skills Wet Lab Techniques
- Cell Culture: maintenance of cells, revival, passage, cryo-
preservation, established 3D cell culture, MTT assay, Cell
migration assay, Chick fibroblast culture.
- Molecular Biology: DNA and RNA isolation, restriction
digestion of DNA, ligation, Competent cell preparation, Cloning,
DNA fragmentation assay, c-DNA, PCR, Primer optimization,
SDS-PAGE, Western Blot, 2D-gel electrophoresis
- Chromatography: ion exchange, gel filtration, affinity.
- Instrumentation: Light microscope, Leica phase contrast
microscope, spectrophotometer, Iinvitrogen’s Qubit 2.0
fluorometer, ELISA plate reader, PCR.
Dry Lab Techniques
- Basic Bioinformatics: BLAST, FASTA, SPDB viewer, KEGG,
Primer designing.
PUJA SARKAR
MS -Biotechnology specialization in Stem Cell Biology
pujasarkar1401@gmail.com
Mob: +91-7304083469
January 2013-June 2014
July 2014- December 2014
2. Projects - Title: “Niche induces de-differentiation”
Duration: 1year (Aug 2011- Aug 2012)
Place: IGNOU-I2IT CoE for Advanced Education and
Research, Pune.
- Title: “Effect of microtubule associated proteins on microtubule
nucleation”
Duration: 3 months (20 December2009 to 19 March 2010)
Place: Rajiv Gandhi Centre For Biotechnology, Trivandrum.
Publications - Calcium sensing receptor modulation for cancer therapy.
Sarkar P, Kumar S.Asian Pac J Cancer Prev.2012;13(8):3561-8.
- Niche Induces Dedifferentiation. Sarkar P, Kapoor A, Yadav
JN, Kumar S (2013) J Bioanal Biomed 5: 102-107.
- Tissue Specific Role of Calcium Sensing Receptor. Sarkar P,
Kumar S. (2013) Atlas Genet Cytogenet Oncol Haematol.
- Venoms can be a Boon for Cancer Patients. Kumar S, Sarkar
P, Jain R.(2014)/ForumImmunDisTher.
- Induced Pluripotent Stem Cells: A New Hope for Treatment
of Degenerative Diseases. Sarkar P, Desai S, Yadav JN, Kumar
S. Current Pharmaceutical Biotechnology (under review).
Poster Presentations - Presented a poster titled “Effect of extract of plant parts of
Curcumin longa on HeLa” in Annual Biotechnology
Conference for Students (ABCS), 2011.
- Presented a poster titled “Effect of extract of leaves of
Azadirachta indica on HeLa and K562 cell lines” in Annual
Biotechnology Conference for Students (ABCS), 2011.
In Plant Training 1 month (June 2008) training in molecular and rDNA technology
at Department of Biotechnology, Bengal Institute of Technology,
Kolkata during 2nd year of B.Tech.
Education IGNOU – I2IT CoE for Advanced Education and Research, Pune, India
MS in Biotechnology with specialization in Stem Cell Biology
Percentage: 79%
SRM University, Chennai, India.
B. Tech in Genetic Engineering
CGPA: 8.87/10
Kendriya Vidyalaya No.2, Kolkata, West Bengal, India.
Percentage: 70%
2006-2010
2010-2012
2005-2006
3. Honours and Awards - Has been awarded entry level scholarship for Master’s program in
the year 2010.
- Awarded with merit scholarship for I and II semesters in Master
program for the year 2011.
Extra Curricular Activity - Event co-coordinator in International Conference on Stem cells
and Cancer (ICSCC), 2010, held at International Institute of
Information Technology, Pune.
- Event co-coordinator in Annual Biotechnology Conference for
Students 2010 and Annual Biotechnology Conference for
Students 2011 held at International Institute of Information
Technology, Pune.
- Industrial visit to Vansant Dada Sugar institute during 1st year
MS
- Industrial visit to Biocon and Metahelix during 3rd year B.tech
- Volunteer at National Conference on Recent Advances in
Bioengineering held at SRM University, Chennai.
- Volunteer at GATC-07, a National Conference held at SRM
University, Chennai.
Referees Dr. Sudhir Kumar.
Email: sudhir2003@gmail.com
Associate Professor,MGM's, Institute of Biosciences & Technology,
N-6 CIDCO,MGM Hospital Campus, Aurangabad,India.
Dr Rajeev Jain.
Email: rageevjain@gmail.com
Postdoctoral Researcher, Department of Pharmacology
University of Tartu, Ravila 19, Tartu 51014. Estonia.
Dr. Prasad Burra VLS.
Email: dr.prasad.bvls@gmail.com
Professor, MGM's, Institute of Biosciences & Technology,
N-6 CIDCO,MGM Hospital Campus, Aurangabad, India
Dr. Sangita Venkataraman
Email: dr.sangita.b@gmail.com
Associate Professor,MGM's, Institute of Biosciences & Technology,
N-6 CIDCO,MGM Hospital Campus, Aurangabad,India.
I hereby certify that the information are true and correct to the best of my knowledge and belief.
Puja Sarkar