Proteomic study of leaves by Puja Ghosh

1. PROTEOMIC
STUDY OF PLANT
LEAVES
PRESENTED BY
PUJA GHOSH
M.PHARM IN PHARMACOGNOSY
SUPERVISOR
DR. MAHENDRA RANA

2. OMICS STUDY
 Omics refers to a field of study in biology ending in -omics,
such as genomics, proteomics, metabolomics.
 Omics aims at the collective characterization and quantification
of pools of biological molecules that translate into the structure,
function, and dynamics of an organism or organisms.

3. OMICS STUDY

4. TECHNIQUES IN OMICS STUDY

5.  Diagnosing and screening of the disease.
 To understand the normal physiological process.
 To discover biomarker in multiple molecules for the future prospective.
 Helpful in the discovery process of drug and in toxicity of drug.
 Pharmacogenomic which mainly defines the role of the genome in
response to the drug.
 Pharmacogenomic role in cancer therapy is very important in toxicity and
efficacy of drug.
 Obesity, cancer and cardiovascular disease.
APPLICATION OF OMICS STUDY

6.  Total protein which is expressed in a tissue or cell of an organism is known
as proteome. Proteomic study deals with the protein in the cell, tissue, or
organism and function of such proteins and the study of protein of an
organism is called proteomic study.
 Immunoproteomics: study of large sets of proteins (proteomics)
involved in the immune response
 Nutriproteomics: Identifying the molecular targets and Uses mass
spectrometry data for protein expression studies
 Proteogenomic: Intersection of proteomics and genomics and used for
gene annotations.
 Structural genomics: Study of 3-dimensional structure of every protein.
PROTEOMICS STUDY

7. TYPES OF PROTEOMICS

8. APPLICATION OF PROTEOMICS
Proteomics
Tumor
Metastasis
Neurotrauma
Discovery Of
Protein
Biomarkers
Neurology
Disease
Diagnosis
Cardiovascular &
Diabetes
Research
Autoantibody
Profiling
Treatment Of
Autoimmune
Disease
Urological
Cancer Research
Fetal & Maternal
Medicine
Nutrition
Research
Protein
Expression
Profiling

9. LEAF Liquid nitrogen added to plant leaves to
turned into fine powdered
Extracting buffer / Lysis buffer added to100mg
of leaf powder in Eppendorf tube
Incubated at 37 temp, for 15 min
Kept for centrifugation for 10 min
Supernatant collected and kept for
centrifugation again with extraction buffer for
purification purpose
Upper phase (supernatant) collected,
which is protein
Stored at 4 temp

10. EXTRACTION OF PROTEIN

11. ELECTROPHORESIS
 The migration of charged ions in an electric field, The gel
itself is composed of either agarose or polyacrylamide.
 In contrast to proteins, which can have either a net
positive or net negative charge, nucleic acids have a
consistent negative charge imparted by their phosphate
backbone, and migrate toward the anode.

12. ELECTROPHORESIS

13. PROTEIN FRACTINATION
 Protein to accelerate towards either the cathode or the
anode, depending on the sign of its charge.
 Sodium Dodecyl Sulphate –Polyacrylamide Gel
Electrophoresis (SDS-PAGE) is used for Protein
fractionation.

14. PROTEIN FRACTINATION

15. PROTEIN FRACTINATION

16. TECHNIQUES IN PROTEOMICS
Protein
identification
Post translation
modification
Gel Electrophoresis
Mass Spectroscopy
X-ray
crystallography
Protein
sequencing
Protein
Mapping

17. TECHNIQUES IN PROTEOMICS