Application of proteomic sciences

1. Applications of Proteomics
Science
Sukanya m. Korewar
M.Pharma ( pharmacology )

3. Proteomics
 Is the study of composition ,structure , function and interaction of the
proteins directing the activity of each living cell .
 Types of proteomics
1. Interaction proteomics : protein –protein interaction Used to
identify binary proteins interaction ,protein complexes and interactome .
2. Expression proteomics : protein quantification . The level of any protein in
the cell at any given time is controlled by –
 Rate of transcription of the gene .
 Rate of degradation of protein
 Efficiency of translation of mRNA into the protein

6. Application of proteomics
 Manufacturing and production of potential new drug for treatment of diseases
Eg: Diabetes ,cancer
 This Depends upon genome an information in the form of protein expression .
 Manufactured drug to inactive protein involved in disease
 Computer software used as target for new drug discovery
 Competitive molecule that inactivates the enzyme
 Forms basic new drug discovery tool

8. Genomics
 Is an interdisciplinary field of science focusing on the structure ,function
,evolution ,mapping and editing of genomes .
 A genome is an organism’s complete set of DNA ,including all of its genes .
 Its involve the study of all genes at the DNA and proteome level as well as the
cellular tissue level .

9. Applications of genomics
 Molecular medicine
 Genes for disease succeptibility
 Microbial genomics
 Risk assessment
 Bioarchaelogy ,anthropology ,evolution
 DNA identification, forensic
 Agriculture ,breeding bioprocessing
 Increased rate of genetic improvement
 Detect abnormalities
 Animal cloning
 Transgenic animals

11. Metabolomics
 It is the large scale study of small molecule
 Metabolites within the cells , biofluids ,tissue ,or organisms .
 Collectively this small molecules and their interactions within a biological
system are known as metabolome

13. Applications of metabolomics
 Pharmacology and pre-clinical drug trials
 Toxicology
 Transplant monitoring
 New born screening
 clinical chemistry
 Tool for functional genomis

15. Nutrigenomics
 It is the branch of nutritional genomic and is the study of effect of the food
constituents on gene expression .
 It will identify the gene involved in physiological responses to diet and the
gene in which small changes ,called polymorphism and the influence of
environmental factors on gene expression .

18. Application of Nutrigenomics
 Cancer treatment
 Obesity
 Ageing
Cancer
 Nurtigenomics may be able to supplement current oncology .
 That prevent cell abnormalities linked to cancer and certain nutrients that
play a role as cofactors .
 Genome damaged caused by micronutrient deficiency may be just as severe
damage to exposure to certain environmental carcinogens .
 If these micronutrients can be identified, with evidence ,risk of cancer can be
significantlly reduced individually.
 Eg –Folate different conc. Less damage to chromosome
due to heavy amount of radiations .

19.  AGEING
 Ageing of cell occur because of the accumulation of excess free radicals due
to lack of nutrition to the cell an external factors such as UV rays ,pollution
,stress ,etc .
 DNA analysis is instrumental in identifying the right nutrient needed to
eliminate excess free radicals .
 OBESITY
 Due to genetic variations among individuals each person could respond to diet
diffentaly .
 By exploring the interactions between dietary pattern and genetic factors
 Nutrigenomics aims to suggest prevention measures and/ or treatment to
obesity in personal nutrition