Good laboratory practices, objective GLP, Mission GLP, Safety practices in laboratory, Happy lab, hazards in laboratory

1. Good Laboratory Practices
(GLPs)
Dr. Priya P. Gurav
Scientist
ICAR- Indian Institute of Soil Science, Bhopal

2. Good Laboratory Practices (GLP)
 GLP embodies a set of principles that
provides a framework within which
laboratory studies are planned performed,
monitored, reported and archived.
 Applies to non-clinical studies.
 GLPs must not be confused with standards
of laboratory safety like wearing lab coats
etc.

3. WHY WAS GLP CREATED?
1. Poor laboratory practice.
2. Equipment not been calibrated to standard form ,therefore
giving wrong measurements.
3. Incorrect/inaccurate accounts of the actual lab study.
4. Inadequate test systems

4. Objectives of GLP
• GLP makes sure that the data submitted are a true reflection of the
results that are obtained during the study.
• GLP also makes sure that data is traceable.
• Promotes international acceptance of tests.

5. To certify that every step of the analysis is
VALID
Goal

6. Mission of GLP
• Test systems
• Standard operating procedures (SOP)
• Personnel and test facility organization
• Archiving of records and materials
• Apparatus, material and reagent facilities
• Quality assurance programs
• Performance of the study
• Reporting of study results

7. Good Laboratory Safety
Practices

9. • When performing potentially hazardous/dangerous operations
always:
– Make sure someone is in the laboratory with you
– Notify someone that you are in the lab and what time you will check
with them when you complete your work.
– Check-in with that person if you exceed your time estimate or if you
leave.
Work as a Team

10. A Clean Lab is a Happy Lab

11. A Cluttered Lab is a Dangerous Lab!
Keep your lab clean!

12. Housekeeping
The Results of Poor housekeeping:
• Accidents
– Messy counter tops are a good source of spills.
– Poorly maintained equipment or apparatus can break
spilling its contents, damage other equipment or flying debris
can cause injury.
– Containers and equipment on the floor can cause hazard.

13. The Results of Poor housekeeping:
• Costs you money
– Overstocking or reordering of
chemicals due to uncontrolled
or unmanaged inventory.
– Replacing spilled chemicals or
damaged equipment.
Housekeeping

14. Housekeeping
The Results of Poor housekeeping:
• Contaminates your research
– Poorly washed containers or equipment
can lead to cross contamination.
– Poorly labeled storage
containers can lead to
the use of a wrong
reagent.

15. Housekeeping
Poor housekeeping:
• Causes accidents:
• Costs you money
• Contaminates your research
• Sets up bad habits for students
entering a more regulated
industrial setting.
• Gets more attention from
inspectors.

16. Don’t Block Access to Electrical Panels, Safety
Showers and Eye Wash Stations

17. Don’t block doors or Fire Extinguishers

18. Old Equipment Can Be Dangerous
Rusty water-bath with exposed wires
Maintain your
Equipment!

19. Improper Chemical Storage
• Ether was stored in
domestic style
refrigerator. Either
the thermostat or the
interior light was the
ignition source that
caused the explosion.
• Flammables have to be
stored in a Flammables
or Explosion proof
fridge.

20. Lack of Attention to Details
Heater/stir plate
being used to stir
only accidentally
was also turned on
as hot plate also!

21. Incomplete reaction placed in
refrigerator
Reaction container was placed into the refrigerator
before the reaction had been completely quenched.
The bottle over pressurized and exploded.

22. Do Not Store Consumables with
Chemicals!

23. Proper Equipment Usage
• There needs to be a written Standard Operating
Procedure for all equipment
• Everyone should be properly trained before using any
equipment
• Improperly used or maintained equipment can be
dangerous to your health or can result in expensive
damages
• Fires are common result with inappropriate equipment
usage

24. Centrifuges- An Example of Improper Usage
and Maintenance

25. Hazards
• Mechanical failure of rotating parts (often violent).
• Contact with rotating parts.
• Sample leaks causing aerosols, stress corrosion,
contamination.
• Sample imbalance causing machine movement / walking (or
stress failure of component parts).
• Fire or explosion.
• Health (contact with contaminated components / vapors).

26. Rotor Failure

27. Rotor Failure

28. Rotor Failure

29. Dancing Centrifuge
This can be caused by an improperly balanced rotor.

31. • Conduct yourself in a responsible manner at all times in the
laboratory
• Follow all written and verbal instructions carefully. If you
do not understand a direction or part of a procedure, ASK
YOUR TEACHER BEFORE PROCEEDING WITH THE
ACTIVITY
• Never work alone in the laboratory. No student may work in
the science classroom without the presence of the teacher
• When first entering a science room, do not touch any
equipment, chemicals, or other materials in the laboratory
area until you are instructed to do so
• Perform only those experiments authorized by your
teacher. Carefully follow all instructions, both written and
oral. Unauthorized experiments are not allowed

32. • Do not eat food, drink beverages, or chew gum in
the laboratory. Do not use laboratory glassware as
containers for food or beverages
• Be prepared for your work in the laboratory. Read
all procedures thoroughly before entering the
laboratory. Never fool around in the laboratory.
Horseplay, practical jokes, and pranks are
dangerous and prohibited
• Always work in a well-ventilated area
• Observe good housekeeping practices. Work areas
should be kept clean and tidy at all times
• Be alert and proceed with caution at all times in the
laboratory. Notify the teacher immediately of any
unsafe conditions you observe

33. • Dispose of all chemical waste properly. Never
mix chemicals in sink drains. Sinks are to be
used only for water. Check with your teacher
for disposal of chemicals and solutions
• Labels and equipment instructions must be
read carefully before use. Set up and use the
equipment as directed by your teacher
• Keep hands away from face, eyes, mouth, and
body while using chemicals or lab
equipment. Wash your hands with soap and
water after performing all experiments.

34. • Experiments must be personally monitored at all
times. Do not wander around the room, distract
other students, startle other students or interfere
with the laboratory experiments of others
• Know the locations and operating procedures of
all safety equipment including: first aid kit(s), and
fire extinguisher. Know where the fire alarm and
the exits are located
• Know what to do if there is a fire drill during a
laboratory period; containers must be closed, and
any electrical equipment turned off
• Any time chemicals, heat, or glassware are used,
students will wear safety goggles. NO
EXCEPTIONS TO THIS RULE!

35. • Contact lenses may be not be worn in the laboratory
• Dress properly during a laboratory activity. Long hair,
dangling jewelry, and loose or baggy clothing are a hazard in
the laboratory. Long hair must be tied back, and dangling
jewelry and baggy clothing must be secured. Shoes must
completely cover the foot. No sandals allowed on lab days

36. • A lab coat or smock should be worn during
laboratory experiments
• Report any accident (spill, breakage, etc.) or
injury (cut, burn, etc.) to the teacher immediately,
no matter how trivial it seems. Do not panic
• If a chemical should splash in your eye(s) or on
your skin, immediately flush with running water
for at least 20 minutes. Immediately (and loudly)
yell out the teacher's name to get the teacher's
attention.

37. • All chemicals in the laboratory are to be considered
dangerous. Avoid handling chemicals with fingers. Always
use a tweezers. When making an observation, keep at least 1
foot away from the specimen. Do not taste, or smell any
chemicals.
tweezers
• Check the label on all chemical bottles twice before
removing any of the contents. Take only as much chemical
as you need
•Never return unused chemicals to their
original container

38. Never remove chemicals or other materials from
the laboratory area.
Never handle broken glass with your bare
hands. Use a brush and dustpan to clean up
broken glass. Place broken glass in the
designated glass disposal container
Examine glassware before each use. Never
use chipped, cracked, or dirty glassware
If you do not understand how to use a piece of
equipment, ASK THE TEACHER FOR
HELP!
Do not immerse hot
glassware in cold
water. The glassware
may shatter.

39. • Do not operate a hot plate by yourself. Take care that hair,
clothing, and hands are a safe distance from the hot plate at all
times. Use of hot plate is only allowed in the presence of the
teacher
• Heated glassware remain very hot for a long time. They should
be set aside in a designated place to cool, and picked up with
caution. Use tongs or heat protective gloves if necessary
• Never look into a container that is being heated
• Do not place hot apparatus directly on the
laboratory desk. Always use an insulated
pad. Allow plenty of time for hot apparatus to
cool before touching it

42. Remember Good Practices
• Never work alone.
• Commit to good housekeeping
• Do not block access to doors, electrical or emergency
equipment
• Store chemicals and wastes properly
• Maintain equipment