 A spectrophotometer is an instrument that measures the amount of photons absorbed by a sample after it is passed through its solution.  UV-Visible spectrophotometer uses UV and visible range of electromagnetic radiation spectrum.

1. UV-VISIBLE
SPECTRO
PHOTOMETER

2. INDEX
 Introduction
 Principle
 Instrumentation
 Types
 Application

3. INTRODUCTION
 A spectrophotometer is an instrument that measures the amount of
photons absorbed by a sample after it is passed through its solution.
 UV-Visible spectrophotometer uses UV and visible range of
electromagnetic radiation spectrum.
 Electromagnetic spectrum:

4. PRINCIPLE
 BEER – LAMBERT LAW
It states that the absorbance of light by a material in a solution is directly
proportional to its concentration in that solution.
A = ϵlc
Where,
 A – absorbance
 ϵ - molar absorptivity
 l – length of solution
 c – concentration

5.  Transmittance (T) = It / Io
 A = log1/ T = log Io / It

6. INSTRUMENTATION

7. TYPES
Single beam

8. Double beam

9. APPLICATIONS
 1. Concentration measurement
 2. Detection of impurities
 3. Chemical kinetics
 4. Detection of functional group

10. Thank you