Research and potential impact of the development of an effective lure for Queensland Fruit fly (Q-fly), Bactrocera dorsalis.

1. biosecurity built on science
Development of a female Qfly lure
Andrew Finlay, Summerfruit Australia
&
Paul Cunningham
Research Leader, AgriBio, DEDJTR, VIC.

2. biosecurity built on science
Female Qfly Lures
Feeding
Egg laying
Bactrocera tryoni

3. biosecurity built on science
Phase 1: Proof of concept
Blends containing fruit ripening
volatiles attract female Qfly
Proof of concept 
11V
• analytical chemistry
• blend formulation
• electrophysiology
• cage trials
A SYNTHETIC FRUIT ODOUR TO
ATTRACT MATED FEMALE FLIES

4. biosecurity built on science
Phase 2: Formulations & Field studies
Finding the right mix

5. biosecurity built on science
Phase 2: Formulations & Field studies
Finding the right mix
FEMALE PHEROMONES
Day 12 mated
Day 3 virgin
Deterrent?
• Solvent extraction
• Rectal gland extracts
• Insect emissions

6. biosecurity built on science
Phase 2: Formulation & Field studies
Finding the right mix
YEASTS Cryptococcus flavescens
Aureobasidium pullulans
Rhodotorula sp (2)
Hannaella sp (3)
Pichia fermentans
Sydowia eucalypti
C. flavescens

7. biosecurity built on science
Phase 2: Formulation & Field studies
Finding the right mix
Deterrents
• Females avoid larval infested fruit
• Acetoin emissions

8. biosecurity built on science
Phase 2: New technologies
Applying calcium imaging of the antennal
lobe to insect lure development
Volatile-evoked responses
in the Qfly antennal lobe
Qfly brain
Improved methods for
attractant formulation
across insect pests
• blend synergy
• volatile detection
• valence

9. biosecurity built on science
Fieldwork 2016/17
Field trials:
2. Mated vs unmated capture
rates
• feeding vs egg laying lures
1. Trial lure formulations
• x 4 formulations
• New dispensers
Tatura (VIC)
Stanthorpe (QLD)

10. biosecurity built on science
Benefits of female lure research
Commercial fruit growers / consultants
 Monitoring / surveillance
 Reducing Qfly damage
 Lowering within / between season populations
 Reducing urban Qfly populations
 Improved market access
 Reduction in production losses
Sterile Insect Technique
 Assessment of success
 Lowering populations
Scientists
 Olfactory research
 Developing new technologies for pest management

11. biosecurity built on science
Thank you
Project Team
Jane Royer (QDAF)
Pawel Sadowski (QUT/CARF)
Mikael Carlsson (Stockholm University, AL imaging)
Tony Clarke (QUT)
Tommaso Villa (female pheromones)
Owen Webb (acetoin emissions)
Alex Piper (yeast analysis)
Summerfruit Australia
Andrew Finlay (Chairman)
Rowan Berecry