Preparation and uses of Anti viral principles

1. Antiviral principles (AVP)
N. H. SHANKAR REDDY
Ph.D., Plant Pathology
Annamalai University

2.  Presence of certain compounds in plants leaves possess inhibitory action against
virus are known as Anti-viral principles (AVP) or Anti-viral factors (AVF).
 Eg - The leaf extracts of sorghum, coconut, bougainvillaea, Prosopis juliflora and
Cynodon dactylon are known to possess these properties.

3. Preparation of AVP extract
• Dried sorghum leaves are collected and powdered. 20 kg of leaf powder is mixed in
50 litres of water and heated for 60o C for one hour.
• Filter the solution and make it into 200 lit. This contains 10 % extract. 500 litres of
spray solution are required for 1 ha.
• Two sprays are given at ten and twenty days after sowing.

4. Management of pathogens
 The leaf extracts of sorghum is effective against groundnut bud necrosis virus,
whereas 10 % leaf extracts of Prosopis juliflora and Cynodon dactylon effectively
reduced the tomato spotted wilt virus in tomato.
 The anti-viral protein (AVP) extracts from Bougainvillea spectabilis and Prosopis
chilinesis were found to be effective in reducing the sunflower necrosis virus
(SFNV).
 The leaf extracts are known to contain some proteinaceous substances which induces
viruses in plants.

5. • Mechanism of action – These antiviral proteins extracted from plants are induced
by living cells to interfere with viral replication.
• Inhibits the virus replication in a host cell and movement to other cells.

6. Video available at you tube
Channel – Geeky Researcher