Activity of isoflavonoids present in seeds of Millettia ferruginea against microorganisms and blood mononuclear cells

1. Activity of isoflavonoids present in seeds
of Millettia ferruginea against
microorganisms and blood mononuclear
cells
Dr. Diganta Dey
Ashok Laboratory Clinical Testing Centre Pvt. Ltd.

2. Millettia ferruginea (Hochst.) Baker
• 25 m high endemic plant of Ethiopia
belonging to Fabaceae (Leguminosae) family
• Traditional usage of seed for controlling
storage pests
• Leaves and shoots taken as fodder for
ruminants
• Rich in pyrano- and C-prenylated isoflavones
with potential pharmacological activity

3. Study Protocol
M. ferruginea seeds
Chloroform/ Methanol/
Water
Antibacterial activity
Antifungal activity
Antitubercular activity
Antileishmanial activity
Barbigerone/ Calopogonium isoflavone-A/
Durmillone
Antibacterial activity
Antifungal activity
Antileishmanial activity
DNA-fragmentation effect on PBMC
Solvent
extraction
Spectroscopic
analysis

4. Antibacterial Activity

5. Antifungal Activity
Antitubercular Activity

6. Antileishmanial Activity

7. DNA-fragmentation Effect of Isoflavonoids
Comet assay for estimation of DNA
damage in peripheral blood mononuclear
cells (PBMC): (A) Untreated; (B) 100 µM
Hydrogen peroxide treated; (C) 1 µM
barbigerone treated; (D) 1 mM
barbigerone treated; (E) 1 µM
calopogonium isoflavone-A treated; (F) 1
mM calopogonium isoflavone-A treated;
(G) 1 µM durmillone treated; (H) 1 mM
durmillone treated cells.
E
A B C
D F
G H

8. DNA-fragmentation Effect of Isoflavonoids
E
A B C
D F
G H
Estimation of the percentage of DNA
present in comet tail. 100 µM Hydrogen
peroxide treated PBMCs were taken as
positive control. Data presented as mean ±
standard error of mean. *p < 0.05 and ***p
< 0.001 considered as significant difference
compared to the untreated control group.

9. Discussion
• Antibacterial efficacy of M. ferruginea seed extracts (water and
methanol) was observed against both Gram-positive and Gram-negative
ATCC strains
• Barbigerone, calopogonium isoflavone-A and durmillone isolated from
the seeds were found to exhibit inhibitory activity against Gram-negative
ATCC strains and L. donovani promastigotes for the first time
• Comet assay was conducted on PBMC and pretreated with the three
isoflavonoids in order to evaluate the possibility of DNA fragmentation
induced by the compounds. The result showed that the compounds were
safe to be considered for further therapeutic application