BLAST is a commonly used tool for comparing gene and protein sequences that searches databases for local alignments to a query sequence. It is available on the NCBI website and others. BLAST outputs alignments in formats like HTML and can compare nucleotide or protein sequences. It has applications like identifying species, domains, and phylogeny.
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Blast
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2. Definition
Introduction
NCBI home page
NCBI-BLAST home page
EMBL-EBI BLAST page
BLAST Input & Output
Type of BLAST
BLAST Function
Objectives of BLAST
3. The Basic Local Alignment Search
Tool (BLAST) for comparing gene
and protein sequences against
others in public databases.
BLAST is a set of sequence
comparison algorithms used to
search databases for optimal local
alignments to a query.
4. Today, one of the most commonly used tools
to examine DNA and protein sequences is the
Basic Local Alignment Search Tool, also
known as BLAST.
BLAST is a computer algorithm that is
available for use online at the National Center
for Biotechnology Information (NCBI) website
and many other sites
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8. Input
Input sequences
In FASTA or Genbank format.
Output
BLAST output can be
delivered in a variety of
formats. These formats
include HTML,plain text, and
XML formatting. For NCBI's
web-page, the default format
for output is HTML
9. Nucleotide-nucleotide BLAST (blastn)
Compares nucleotide query
against nucleotide sequence
database
Protein-protein BLAST (blastp)
Compares protein query
against proteins sequence
database.
10. Large numbers of query sequences (megablast) :
When comparing large numbers of input
sequences via the command-line BLAST,
"megablast" is much faster than running
BLAST multiple times.
Of these programs,BLASTn and BLASTp are
the most commonly used because they use
direct comparisons, and do not require
translations.
11. BLAST can be used for several
purposes. These include :
Identifying species,
locating domains,
Establishing phylogeny,
DNA mapping,
Comparison.
12. It is one of the most popular programs for
sequence analysis.
Enables a researcher to compare a query
sequence with a library or database of
sequence.
Alignments of the high-scoring segment
pairs showing identical and similar residues.
