Introduction of Hemocytometry.

1. Mukul Sharma
Faculty of Pharmacy, Medi-caps University
mukul.sharma@medicaps.ac.in
Hemocytometry

2. Introduction to Hemocytometry
Presented by
Mr. Mukul Sharma
Assistant Professor
Faculty of Pharmacy, Medi-Caps University
B. Pharm First Semester
Human Anatomy Physiology-I
PY3CO01
Prof. Mukul Sharma, Medi-Caps University, Indore

3. Objectives
The students will be able to understand
• Scientific Terms and their Meanings
• Principle and application of hemocytometry
• Learning counting of RBCs and WBCs.
Prof. Mukul Sharma, Medi-Caps University, Indore

4. Experiment No.06
Object: Introduction to Hemocytometry.
References:
• Waugh Anne, Grant Allison, Ross & Wilson: Anatomy and physiology in
health & illness 2006, edition 10th, Elsevier publication, 58
• Tortora GJ, Derrickson B, Principles of anatomy & physiology 2007,
edition 11th, John wiley & sons. Inc., 672
• Deshmukh Pradeep, Practical anatomy, physiology & health education –I
2009, 1st edition. Published by Shree Sai Prakashan, 10-13
• Kale SR, Kale RR, Practical human anatomy and physiology 2007,
edition 7th, Nirali Prakashan, 9.
Prof. Mukul Sharma, Medi-Caps University, Indore

5. Terms:
• Hemocytometry: It is a technique in which microscopic cells like
RBCs, WBCs and platelets can be count either manually or
automatically.
• Dilution: It is process by adding excess solvent like water or other in
solution to lower the concentration of solute.
• Dilution factor: It is a notation in which how much of the original
stock solution present in total solution.
Prof. Mukul Sharma, Medi-Caps University, Indore

6. Material Required:
Requirement: Blood lancet, RBC and WBC diluting solutions,
Neubauer Slide, Microscope, Cotton guage, RBC and WBC pipette.
Prof. Mukul Sharma, Medi-Caps University, Indore
Lancet
RBC and WBC pipette
Neubers counting
slide
Compound
Microscope

7. Prof. Mukul Sharma, Medi-Caps University, Indore
Diluting Fluids

8. Hemocytometer
• It is a counting chamber device originally designed and usually used
counting blood cells
Prof. Mukul Sharma, Medi-Caps University, Indore
Neubers slide

9. Prof. Mukul Sharma, Medi-Caps University, Indore
Neubaurs Chamber with coverslip

10. Prof. Mukul Sharma, Medi-Caps University, Indore

11. Prof. Mukul Sharma, Medi-Caps University, Indore
Counting Square for WBC At 10X Counting Squares for RBC At 10X

12. Applications:
• Count RBCs and WBCs in blood samples.
• Find infections and healthy conditions in body.
Prof. Mukul Sharma, Medi-Caps University, Indore

13. Precautions
• Clean the prick area properly using cotton with spirit.
• Don’t overflow the chamber, cover with cover-slip.
• Avoid air-bubbling in pipette.
• Avoid coagulation of blood inside the pipette.
• Clean the pipette and Neubaurs slides after the practical with 0.1 N HCl
solution.
• Spirit is used for cleaning and antiseptic purposes.
• Never use lancet multiple times. Discard immediately after use with proper
capping.
• All waste materials like cotton plucks, blood sample in waste bins.
Prof. Mukul Sharma, Medi-Caps University, Indore

14. Result:
Learnt the concept and principle of Hemocytometer.
Prof. Mukul Sharma, Medi-Caps University, Indore