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INSECT RESISTANT
COTTON
Muhammad Waseem
2018-ag-109
Seminar - 720
Introduction
■ Cotton is a soft, fluffy staple fiber.
■ Cash crop- known as “White Gold”.
■ King of fiber crops
■ 4th in ranking (Bakhsh et al., 2009; Sial et al., 2014)
■ Gossypium in the mallow family Malvaceae.
Economic Value
(PBS 2018-19)
■ Agriculture contributes 18.5% to country’s
Gross Domestic Product (GDP).
■ Considered as life line of economy of
Pakistan.
■ 0.8% share in GDP
■ And contributes 4.5% in agriculture value
addition.
Cotton (Bales)
2012-13 2013-14 2014-15 2015-16 2016-17 2017-18 2018-19
13031 12769
13960
9917
10671
11946
9861
ECONOMIC SURVEY 2018-19
Cotton (Bales)
Major Insect Pests
Sucking Pests
•Whitefly
•Thrips
•Jassid
Chewing Pests
•Pink Bollworm
•American Bollworm
•Spotted Bollworms
•Armyworms
Yield losses due to…
■ Unfavorable weather conditions
■ stunting of crop
■ uncontrol of Whitefly, Pink
Bollworms and other insect pests
■ Fluctuating prices
■ 60% to 80% yield losses due to
insect pests.
Insect infections due to…
■ Most susceptible crop
■ Green and succulent foliage
■ Indeterminate growth habit
■ Presence of extra foliar nectarines
Pesticides ratio:
60% pesticides
use in Cotton
and 40%
pesticides use
in other several
crops.
Cotton
60%
Other Crops
40%
PESTICIDES RATIO
Kang (2013)
Basis of Insect Resistance…
There are 3 basic mechanisms in
Cotton
■ Morphological mechanism
e.g. Hairiness- jassid
■ Physiological mechanism
e.g. Osmotic Concentration-
Resist against Jassid.
■ Biochemical mechanism
e.g. Gossypol
Basis of Insect Resistance…
■ Non Preference
e.g. Hairiness, Fregobracts
in Cotton
■ Antibiosis
e.g. High Gossypol level
■ Tolerance
Hybrid varieties- High
potential for insect resistant
■ Avoidance or Escape
e.g. Early maturing varieties
Sources of Insect Resistance
■ Cultivated varieties
■ Germplasm collections
■ Wild species
e.g. G.tomentosum , G.anomalum & G.armourianum are good
sources of jassid resistance in cotton.
■ Mutations
■ Microorganism
e.g. Bacillus thuringiensis (Bt)
Bt Cotton
■ Genetically modified
crop
■ Bacillus Thuregiensis is a
Bacterium, discovered by
Ishiwatari in 1901.
■ Bacterium produces
insecticidal protein- Cry
Protein.
■ Currently the genetic
modified cotton
cultivation consumed for
more than 95% area
under cotton cultivation
Why Bt Cotton?
Insect resistant
crop. e.g. Bollworm
resistant
Reduces the
insecticides
because of
insecticidal protein
in nature
More effective than
other cotton
varieties
How Bt works…
■ Ingestion
■ Solubilization & proteolytic activation
■ Binding to target site
■ Formation of toxic lesion
Achievements
(Insect resistant varieties)
MNH-552
IR-901
FNH-1000
CIM-473
NIBGE-1
Future Outlook
■ Bt varieties should be
grown
■ High gossypol contents on
plant not on seed
■ Hairiness, waxy leaves
■ Frego bracts
■ Early Maturity Varieties
■ Increase the Terpenes
Contents(terpenoids)
■ Okra leaves
References…
■ Government of Pakistan. Economic Survey (2018– 19) of Pakistan. Ministry of Finance,
Islamabad.
■ Shahid. J (2014) Bollworms develop resistance against cotton crop.
http://www.dawn.com. /news/1119078
■ Kang, S.A. 2013. Impact of pesticide use in Pakistan agriculture: there benefits and
hazards.
■ Khan, S.M., I. Saeed, M. Shah, S.F. Shah and H. Mir. 2012. Intergration of tolerance of
Bt. Cotton varieties with insecticides against spotted bollworm, Earias insulana (Boisd.)
and E. virella (Fab.) (Noctuidae: Lepidoptera). Sarhad J. Agric. 28: 57–62.
■ Bravo A., Gill S. S., & Soberon M. (2007). Mode of action of Bacillus thuringiensis Cry
and Cyt toxins and their potential for insect control. Toxicon, 49(4),423-435.
■ Fujimoto H, ltoh K, Yamamoto M, Kyozuka J, Shimamoto K (1993) Insect resistant rice
generated by introduction of a modified a-endotoxin gene of Bacillus thuringiensis,
Bio/Technology 11:1151-1155.

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Seminar 720

  • 2. Introduction ■ Cotton is a soft, fluffy staple fiber. ■ Cash crop- known as “White Gold”. ■ King of fiber crops ■ 4th in ranking (Bakhsh et al., 2009; Sial et al., 2014) ■ Gossypium in the mallow family Malvaceae.
  • 3. Economic Value (PBS 2018-19) ■ Agriculture contributes 18.5% to country’s Gross Domestic Product (GDP). ■ Considered as life line of economy of Pakistan. ■ 0.8% share in GDP ■ And contributes 4.5% in agriculture value addition.
  • 4. Cotton (Bales) 2012-13 2013-14 2014-15 2015-16 2016-17 2017-18 2018-19 13031 12769 13960 9917 10671 11946 9861 ECONOMIC SURVEY 2018-19 Cotton (Bales)
  • 5. Major Insect Pests Sucking Pests •Whitefly •Thrips •Jassid Chewing Pests •Pink Bollworm •American Bollworm •Spotted Bollworms •Armyworms
  • 6. Yield losses due to… ■ Unfavorable weather conditions ■ stunting of crop ■ uncontrol of Whitefly, Pink Bollworms and other insect pests ■ Fluctuating prices ■ 60% to 80% yield losses due to insect pests.
  • 7. Insect infections due to… ■ Most susceptible crop ■ Green and succulent foliage ■ Indeterminate growth habit ■ Presence of extra foliar nectarines
  • 8. Pesticides ratio: 60% pesticides use in Cotton and 40% pesticides use in other several crops. Cotton 60% Other Crops 40% PESTICIDES RATIO Kang (2013)
  • 9. Basis of Insect Resistance… There are 3 basic mechanisms in Cotton ■ Morphological mechanism e.g. Hairiness- jassid ■ Physiological mechanism e.g. Osmotic Concentration- Resist against Jassid. ■ Biochemical mechanism e.g. Gossypol
  • 10. Basis of Insect Resistance… ■ Non Preference e.g. Hairiness, Fregobracts in Cotton ■ Antibiosis e.g. High Gossypol level ■ Tolerance Hybrid varieties- High potential for insect resistant ■ Avoidance or Escape e.g. Early maturing varieties
  • 11. Sources of Insect Resistance ■ Cultivated varieties ■ Germplasm collections ■ Wild species e.g. G.tomentosum , G.anomalum & G.armourianum are good sources of jassid resistance in cotton. ■ Mutations ■ Microorganism e.g. Bacillus thuringiensis (Bt)
  • 12. Bt Cotton ■ Genetically modified crop ■ Bacillus Thuregiensis is a Bacterium, discovered by Ishiwatari in 1901. ■ Bacterium produces insecticidal protein- Cry Protein. ■ Currently the genetic modified cotton cultivation consumed for more than 95% area under cotton cultivation
  • 13. Why Bt Cotton? Insect resistant crop. e.g. Bollworm resistant Reduces the insecticides because of insecticidal protein in nature More effective than other cotton varieties
  • 14. How Bt works… ■ Ingestion ■ Solubilization & proteolytic activation ■ Binding to target site ■ Formation of toxic lesion
  • 15.
  • 17. Future Outlook ■ Bt varieties should be grown ■ High gossypol contents on plant not on seed ■ Hairiness, waxy leaves ■ Frego bracts ■ Early Maturity Varieties ■ Increase the Terpenes Contents(terpenoids) ■ Okra leaves
  • 18. References… ■ Government of Pakistan. Economic Survey (2018– 19) of Pakistan. Ministry of Finance, Islamabad. ■ Shahid. J (2014) Bollworms develop resistance against cotton crop. http://www.dawn.com. /news/1119078 ■ Kang, S.A. 2013. Impact of pesticide use in Pakistan agriculture: there benefits and hazards. ■ Khan, S.M., I. Saeed, M. Shah, S.F. Shah and H. Mir. 2012. Intergration of tolerance of Bt. Cotton varieties with insecticides against spotted bollworm, Earias insulana (Boisd.) and E. virella (Fab.) (Noctuidae: Lepidoptera). Sarhad J. Agric. 28: 57–62. ■ Bravo A., Gill S. S., & Soberon M. (2007). Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon, 49(4),423-435. ■ Fujimoto H, ltoh K, Yamamoto M, Kyozuka J, Shimamoto K (1993) Insect resistant rice generated by introduction of a modified a-endotoxin gene of Bacillus thuringiensis, Bio/Technology 11:1151-1155.

Editor's Notes

  1. Hot season going prolong, Cotton Crop also affect due to this condition
  2. Physiological; Resist at protein level, Produce special group of proteins phenolic compound which reduce insect population.
  3. Non-preference; Plant resistant mechanism- in which pest may occur but they will not damage the plant. Antibiosis; refers to the adverse effect of host plant on the development and reproduction of insect pests which feed on resistant plant.