Introduction to dissolution apparatus and HPLC instrument

1. Industrial approach on organic chemistry & introduction of HPLC and
dissolution
A WEBINAR REPORT
Submitted to
M.G. SCIENCE INSTITUTE
Department of Chemistry
Affliation : Gujarat university
Submitted in partial fulfilment of the
Requirement for the award of the degree of
MASTER OF SCIENCE
In
Organic Chemistry
Submitted By:-
Roll No:
M.G. Science Institute , Ahmedabad
University Exam No:
Enrollment No:

3. About Workshop
This workshop arranged by Novatoz Scientific LLP. It is a consultancy firm which is limited
liability patnership firm by the the team of pharmaceutical profession established in year
2020.
Industrial approach to organic chemistry involves the application of chemical principles to
the design and production of chemical compounds on a large scale.
Organic chemistry is a key component in the development of many everyday products,
including medicines, plastics, and textiles.
 Industrial chemists focus on optimizing synthetic routes, improving process efficiency, and
minimizing waste to produce high-quality products at a competitive cost.
 This seminar will explore the industrial approach to organic chemistry, including the
various techniques and strategies employed in the synthesis of organic compounds.
It will also examine the challenges and opportunities in this field and its potential impact
on the global economy.

4. TITLE :
Industrial approach on organic chemistry & introduction of
HPLC and dissolution
The speakers of the respective session days introduced their topics and the rest of the
program was started. The speakers and their respective session chairs are as under:
Speakers
Day 1 : Mr. Sandip Patel
Head of Novatoz Scientific LLP.
Day 2 : DR.JANMEJAY DAVE
Head, R&D department, Novatoz scientific. R&D consultant

5. Day1: 19 th Nov 2022
Theme: Introduction of HPLC and dissolution
Speaker : Mr. Sandip Patel
Automated HPLC
1. Automated method scouting techniques allow for efficient development of
(U)HPLC methods.
2. These techniques combine automated multi-column and solvent screening
with intelligent run analysis software.
3. They enable chromatographers to develop effective methods more
rapidly, saving time and resources.
4. Automated method scouting techniques leverage the ultra-fast analytical
speeds of UHPLC.
5. They provide a solution to problems such as lengthy method development
times and high solvent consumption.

6. Dissolution tester
Dissolution testing characterizes the performance of oral solid dosage forms
Rate and extent of drug absorption determined by dissolution from dosage form
Dissolution medium is placed in vessels of a dissolution unit
Medium should be water-based with pH 5-7 at 37ºC and chosen as per product
Degassing the medium is important to avoid affecting results
Drug is placed in medium after it has reached sufficient temperature
Sample solutions collected from dissolution testing are commonly
analyzed by HPLC and UV-visible spectroscopy

7. HPLC systerm Calibration
Column C18 , 150 x 4.6 mm , 5 mm
Mobile Phase Water , Methanol (70.30)
Flow Rate 1.0 ml/min
Injection Volume 20,30,40,50,60 ml
Detector 272 nm
Run Time 7 minutes (at least 1.2 timesof retention of analyte)
Diluent Mobile Phase
1. A 20 g sample of caffeine is dissolved in a 100 ml volumetric flask with 10 ml of mobile phase
and made up to 100 ml
2. 5 ml of the resulting solution is further diluted with mobile phase
3. The HPLC system is stabilized for a constant baseline
4. 20, 30, 40, 50, and 60 ml of a 20 ppm caffeine solution are injected into the system
5. The system solubility criteria are not less than 2000 theoretical plates and not more than 2 for
the tailing factor
6. The obtained result is 0.955, which does not meet the acceptance criteria of not less than
0.999.

8. PREPARATION OF MOBILE PHASE :
Take a mobile phase bottle and wash edit with sterile water
~ Then wash the bottle again with methanol
~ Add 800ml ACN (Acetonitrile)+200ml distilled water in mobile phase
bottle with ratio of 80:20
~Then mix it well
~ Put it in sonicate for 3 to 5min.
~ Then stable it at room temp. for 5 to10min.
~ Here mobile phase solution also used at diluent
PREPARATION OF SAMPLE :
~ Preparation of napthelene
~ Take 10mg of napthelene in a 100ml volumetric flask
~ Add 25ml of diluent for dissolve napthelene
~ Sonicating mixture for proper dissolving
~ After dissolving powder of napthelene make up the volume up to 100ml
with diluent
~ Mix well
Now 1000ppm stock solution of napthelene is ready.

9. Day2: 26th Nov 2022
Theme: Industrial approach on organic chemistry
Speaker : DR.JANMEJAY DAVE
 Research And Development In The Pharmaceutical Industry
1. Pharmaceutical R&D refers to the pharmaceutical research and
development of new medicines. The process begins with under standing
the disease and selecting a target (usually a receptor it on a cell) that can
potentially be affected by a drug molecule.
2.
The regulatory affairs (RA) department of a pharmaceutical company is
responsible for obtaining approved for new pharmaceutical products and
ensuring that approved is maintained for as long as the company wants to
keep the product on the market.

11. Active pharmaceutical ingredient : API

12. Intermidiate synthesis
1. Intermediate synthesis involves synthesizing intermediate compounds in organic chemistry.
2. These intermediates are used as building blocks to synthesize more complex molecules.
3. The target molecule is often too complex to be synthesized in a single step.
4. Intermediate synthesis involves techniques such as protection and deprotection of functional
groups, oxidation and reduction reactions, and the use of various reagents and catalysts.
5. The intermediates are purified and characterized using analytical techniques such as NMR
spectroscopy and mass spectrometry.
6. Optimizing the synthesis of intermediates improves the efficiency and yield of the synthesis.
7. Intermediate synthesis is critical in the development of new drugs, materials, and other
useful compounds.

13. What I Learnt from this workshop ?
1. Understanding the importance of organic chemistry in the development of industrial products
and processes.
2. Understanding the role of HPLC in the analysis and purification of organic compounds in
industrial settings.
3. Familiarity with the principles and techniques of dissolution testing, which is used to measure
the rate at which a drug substance dissolves in a particular medium.
4. Learning about the regulatory requirements and guidelines governing the use of HPLC and
dissolution testing in the pharmaceutical industry.
5. Understanding how to interpret and analyze HPLC and dissolution test results, and how these
results can inform decision-making in industrial organic chemistry.
6. Knowledge of the latest advances and trends in the field of industrial organic chemistry and the
use of HPLC and dissolution testing.
Overall, attendees of such a seminar would gain a better understanding of the crucial role that
organic chemistry plays in industry, and would be better equipped to apply HPLC and dissolution
testing techniques to their own work.