Selective Media for Bacterial Growth

1. Bangabandhu Sheikh Mujibur Rahman
Science & Technology University
Md. Golam Rabbani
Student ID: 16PHR052
Department of Pharmacy
BSMRSTU
Topic: Selective Media for Bacterial Growth

3. Contents
Contents
 Bacteriological Media
 Types of Media
 Selective Media
 Types of Selective Media
Eosin Methylene Blue(EMB) agar based Selective Media
 MacConkey agar based Selective Media
 Mannitol salt agar based selective Media
 Phenyl Ethyl Alcohol (PEA) agar based Selective Media

4. In bacteriological medium where describe,
discuss, analyze about nutritional
requirements for bacterial growth, cultivation
and reproduction.
Bacteriological Media

5. Types of Media
Selective Media
Differential Media
Assay Media
Media for Enumeration of bacteria
Media for Characterization of Bacteria
Maintenance Media
Solid and Semisolid Media

6. Selective Media
These media provide nutrients that
enhance the growth and predominance of a
particular type of bacterium but do not
enhance other types of organism that may
be present.

7. Types of Selective Media
There are some types of selective media-
Eosin Methylene Blue(EMB) agar based Selective Media
MacConkey agar based Selective Media
Mannitol salt agar based selective Media
 Phenyl Ethyl Alcohol (PEA) agar based Selective Media

8. EMB agar based Selective Media
Eosin Methylene Blue agar (EMB agar) contains the dyes
eosin and methylene blue. They inhibit Gram-positive
organisms. Such a medium is selective for Gram-negative
species. Lactose-fermenting organisms such as S.
enterica produce a black precipitate on EMB.
http://www2.highlands.edu/academics/d
ivisions/scipe/biology/labs/rome/selecti
vedifferential.htm

9. MacConkey agar based Selective Media
 MacConkey agar is similar to EMB agar in that it is also
selective for Gram-negative species and differential with
respect to lactose fermentation. MacConkey agar is used
for the detection of coliforms and enteric pathogens based
on their ability to ferment lactose.
http://www2.highlands.edu/academi
cs/divisions/scipe/biology/labs/rom
e/selectivedifferential.htm

10. Mannitol agar based Selective Media
Mannitol salt agar (7.5% NaCl) medium is a selective for
staphylococci and differential with respect to mannitol
fermentation. Growth of most bacteria without
staphylococci, is inhibited by the high concentration of salt
in the medium.
http://www2.highlands.edu/academics/d
ivisions/scipe/biology/labs/rome/selecti
vedifferential.htm

11. PEA agar based Selective Media
Phenyl ethyl Alcohol (PEA) agar is a selective medium
which inhibits the growth of most Gram negative
organisms. Phenyl ethyl alcohol acts to disrupt lipid
structure in the membrane of Gram negative organisms as
well as inhibiting protein synthesis.
http://www2.highlands.edu/academics/d
ivisions/scipe/biology/labs/rome/selecti
vedifferential.htm